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Promega pmirglo vectors (wt or mut)
Pmirglo Vectors (Wt Or Mut), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmirglo+vectors/pmirglo+vector/pm40639725-237-1-15
Average 90 stars, based on 1 article reviews
pmirglo vectors (wt or mut) - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Mutagenesis:

Article Title: tRF ‐34‐ P4R8YP9LON4VHM Promotes Hepatocellular Carcinoma Progression and Tumour Cell‐Induced Angiogenesis via the MEK / ERK Pathway
Article Snippet: .. As DAB2IP was the target of tRF‐34‐P4R8YP9LON4VHM, we used the mutant (MUT) and wild type (WT) of the DAB2IP 3′ UTR to treat PmirGLO vectors (Promega, Madison, WI, USA). ..

Article Title: tRF-34-P4R8YP9LON4VHM Promotes Hepatocellular Carcinoma Progression and Tumour Cell-Induced Angiogenesis via the MEK/ERK Pathway.
Article Snippet: .. As DAB2IP was the target of tRF- 34- P4R8YP9LON4VHM, we used the mutant (MUT) and wild type (WT) of the DAB2IP 3′ UTR to treat PmirGLO vectors (Promega, Madison, WI, USA). ..

Article Title: LncRNA BACE1-AS delays the propagation of Cryptosporidium parvum through regulating cell apoptosis by targeting the miR-6805-5p/IRF3 axis
Article Snippet: .. In this study, the luciferase reporter plasmids of wild-type BACE1-AS (BACE1-AS -WT), mutant BACE1-AS (BACE1-AS -MUT), wild-type IRF3 ( IRF3 -WT), and mutant IRF3 ( IRF3 -MUT) were constructed by cloning BACE1-AS, the miR-6805-5p binding site-mutated BACE1-AS, 3′ UTR region of IRF3 , and the miR-6805-5p binding site-mutated 3′ UTR region of IRF3 into pmirGLO vectors (Promega, Madison, WI, USA), respectively. .. Each abovementioned recombinant plasmid was co-transfected with miR-6805-5p mimics or control mimics into HCT-8 cells by applying Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: LINC00704 facilitates cell proliferation, migration, and invasion via miR-323a-3p/SLC44A1 axis in epithelial ovarian cancer.
Article Snippet: .. Then, the wide-type (Wt) and mutant (Mut) sequences of LINC00704 or SLC44A1 3ʹUTR were amplified and subcloned into pmirGLO vectors (Promega, Madison, WI, USA) to construct LINC00704-Wt/Mut and SLC44A1-Wt/Mut. .. These constructs were then co-transfected with NC mimics, miR-323a-3p mimics, or miR-323a-3p mimics plus pcDNA3.1/LINC00704 into A2780 and SKOV3 cells using Lipofectamine 2000 (Invitrogen).

Article Title: LncRNA XIST enhances gastric cancer cell function by regulating STAT3/PD-L1 axis as a competing endogenous RNA for miR-124
Article Snippet: .. The predicted wild-type (WT) and mutant (Mut) sequences of the XIST binding site for miR-124 were cloned into pmirGLO vectors (Promega, USA). .. MGC803 cells were co-transfected with 10 μg of either pmirGLO-XIST-WT or pmirGLO-XIST-Mut and miR-124 mimics or inhibitors (RiboBio, China) using Lipofectamine 2000.

Article Title: LINC00265 Serves as a Diagnostic and Prognostic Marker for Acute Ischemic Stroke and INHIBITS Disease Progression Through the miR- 155 - 5p/TRIM32 Axis.
Article Snippet: Acute ischemic stroke (AIS) is a medical emergency stemming from a reduction in cerebral blood supply, leading to neuronal injury.. LINC00265 may emerge as a highly promising biomarker for stroke.. The aim of this investigation was to investigate the clinical and prognostic significance of LINC00265 in AIS, as well as to elucidate the molecular mechanisms through which LINC00265 influences AIS.

Clone Assay:

Article Title: Cancer-associated fibroblasts-derived exosomal piR-35462 promotes the progression of oral squamous cell carcinoma via FTO/Twist1 pathway.
Article Snippet: Inhibitor, mimic, as well as plasmid construction The cDNA encoding Fat Mass and Obesity-Associated Protein (FTO) was inserted into the pcDNA3.1-Myc/ His vector (Invitrogen, US) and its sequence was confirmed. .. Wild type (WT) FTO 3’-UTR sequences were cloned into pmiRGLO vectors (Promega, US) for luciferase assays. .. Mutated (MUT) luciferase reporters were created employing PCR-based site-directed mutagenesis. piR-35462 mimics, inhibitors, FTO shRNA, and control sequences were produced by Shanghai GenePharma (China), as outlined in Table 1.

Article Title: LncRNA XIST enhances gastric cancer cell function by regulating STAT3/PD-L1 axis as a competing endogenous RNA for miR-124
Article Snippet: .. The predicted wild-type (WT) and mutant (Mut) sequences of the XIST binding site for miR-124 were cloned into pmirGLO vectors (Promega, USA). .. MGC803 cells were co-transfected with 10 μg of either pmirGLO-XIST-WT or pmirGLO-XIST-Mut and miR-124 mimics or inhibitors (RiboBio, China) using Lipofectamine 2000.

Article Title: LINC01088 Targets miR-195-5p to Promote Proliferation and Migration and Reduce Apoptosis in the Inhibition of Carotid Artery Stenosis.
Article Snippet: .. The partial sequences of LINC0108 containing the putative binding sites of miR-195-5p cloned into the pmirGLO vectors (Promega, Madison, WI, USA). .. The transfection was performed using Lipofectamine 2000 (Invitrogen) and assessed by the dual-luciferase reporter assay system (Promega).

Article Title: LINC00265 Serves as a Diagnostic and Prognostic Marker for Acute Ischemic Stroke and INHIBITS Disease Progression Through the miR- 155 - 5p/TRIM32 Axis.
Article Snippet: Acute ischemic stroke (AIS) is a medical emergency stemming from a reduction in cerebral blood supply, leading to neuronal injury.. LINC00265 may emerge as a highly promising biomarker for stroke.. The aim of this investigation was to investigate the clinical and prognostic significance of LINC00265 in AIS, as well as to elucidate the molecular mechanisms through which LINC00265 influences AIS.

Luciferase:

Article Title: Cancer-associated fibroblasts-derived exosomal piR-35462 promotes the progression of oral squamous cell carcinoma via FTO/Twist1 pathway.
Article Snippet: Inhibitor, mimic, as well as plasmid construction The cDNA encoding Fat Mass and Obesity-Associated Protein (FTO) was inserted into the pcDNA3.1-Myc/ His vector (Invitrogen, US) and its sequence was confirmed. .. Wild type (WT) FTO 3’-UTR sequences were cloned into pmiRGLO vectors (Promega, US) for luciferase assays. .. Mutated (MUT) luciferase reporters were created employing PCR-based site-directed mutagenesis. piR-35462 mimics, inhibitors, FTO shRNA, and control sequences were produced by Shanghai GenePharma (China), as outlined in Table 1.

Article Title: LncRNA BACE1-AS delays the propagation of Cryptosporidium parvum through regulating cell apoptosis by targeting the miR-6805-5p/IRF3 axis
Article Snippet: .. In this study, the luciferase reporter plasmids of wild-type BACE1-AS (BACE1-AS -WT), mutant BACE1-AS (BACE1-AS -MUT), wild-type IRF3 ( IRF3 -WT), and mutant IRF3 ( IRF3 -MUT) were constructed by cloning BACE1-AS, the miR-6805-5p binding site-mutated BACE1-AS, 3′ UTR region of IRF3 , and the miR-6805-5p binding site-mutated 3′ UTR region of IRF3 into pmirGLO vectors (Promega, Madison, WI, USA), respectively. .. Each abovementioned recombinant plasmid was co-transfected with miR-6805-5p mimics or control mimics into HCT-8 cells by applying Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

Construct:

Article Title: LncRNA BACE1-AS delays the propagation of Cryptosporidium parvum through regulating cell apoptosis by targeting the miR-6805-5p/IRF3 axis
Article Snippet: .. In this study, the luciferase reporter plasmids of wild-type BACE1-AS (BACE1-AS -WT), mutant BACE1-AS (BACE1-AS -MUT), wild-type IRF3 ( IRF3 -WT), and mutant IRF3 ( IRF3 -MUT) were constructed by cloning BACE1-AS, the miR-6805-5p binding site-mutated BACE1-AS, 3′ UTR region of IRF3 , and the miR-6805-5p binding site-mutated 3′ UTR region of IRF3 into pmirGLO vectors (Promega, Madison, WI, USA), respectively. .. Each abovementioned recombinant plasmid was co-transfected with miR-6805-5p mimics or control mimics into HCT-8 cells by applying Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: LINC00704 facilitates cell proliferation, migration, and invasion via miR-323a-3p/SLC44A1 axis in epithelial ovarian cancer.
Article Snippet: .. Then, the wide-type (Wt) and mutant (Mut) sequences of LINC00704 or SLC44A1 3ʹUTR were amplified and subcloned into pmirGLO vectors (Promega, Madison, WI, USA) to construct LINC00704-Wt/Mut and SLC44A1-Wt/Mut. .. These constructs were then co-transfected with NC mimics, miR-323a-3p mimics, or miR-323a-3p mimics plus pcDNA3.1/LINC00704 into A2780 and SKOV3 cells using Lipofectamine 2000 (Invitrogen).

Cloning:

Article Title: LncRNA BACE1-AS delays the propagation of Cryptosporidium parvum through regulating cell apoptosis by targeting the miR-6805-5p/IRF3 axis
Article Snippet: .. In this study, the luciferase reporter plasmids of wild-type BACE1-AS (BACE1-AS -WT), mutant BACE1-AS (BACE1-AS -MUT), wild-type IRF3 ( IRF3 -WT), and mutant IRF3 ( IRF3 -MUT) were constructed by cloning BACE1-AS, the miR-6805-5p binding site-mutated BACE1-AS, 3′ UTR region of IRF3 , and the miR-6805-5p binding site-mutated 3′ UTR region of IRF3 into pmirGLO vectors (Promega, Madison, WI, USA), respectively. .. Each abovementioned recombinant plasmid was co-transfected with miR-6805-5p mimics or control mimics into HCT-8 cells by applying Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

Binding Assay:

Article Title: LncRNA BACE1-AS delays the propagation of Cryptosporidium parvum through regulating cell apoptosis by targeting the miR-6805-5p/IRF3 axis
Article Snippet: .. In this study, the luciferase reporter plasmids of wild-type BACE1-AS (BACE1-AS -WT), mutant BACE1-AS (BACE1-AS -MUT), wild-type IRF3 ( IRF3 -WT), and mutant IRF3 ( IRF3 -MUT) were constructed by cloning BACE1-AS, the miR-6805-5p binding site-mutated BACE1-AS, 3′ UTR region of IRF3 , and the miR-6805-5p binding site-mutated 3′ UTR region of IRF3 into pmirGLO vectors (Promega, Madison, WI, USA), respectively. .. Each abovementioned recombinant plasmid was co-transfected with miR-6805-5p mimics or control mimics into HCT-8 cells by applying Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: LncRNA XIST enhances gastric cancer cell function by regulating STAT3/PD-L1 axis as a competing endogenous RNA for miR-124
Article Snippet: .. The predicted wild-type (WT) and mutant (Mut) sequences of the XIST binding site for miR-124 were cloned into pmirGLO vectors (Promega, USA). .. MGC803 cells were co-transfected with 10 μg of either pmirGLO-XIST-WT or pmirGLO-XIST-Mut and miR-124 mimics or inhibitors (RiboBio, China) using Lipofectamine 2000.

Article Title: LINC01088 Targets miR-195-5p to Promote Proliferation and Migration and Reduce Apoptosis in the Inhibition of Carotid Artery Stenosis.
Article Snippet: .. The partial sequences of LINC0108 containing the putative binding sites of miR-195-5p cloned into the pmirGLO vectors (Promega, Madison, WI, USA). .. The transfection was performed using Lipofectamine 2000 (Invitrogen) and assessed by the dual-luciferase reporter assay system (Promega).

Amplification:

Article Title: LINC00704 facilitates cell proliferation, migration, and invasion via miR-323a-3p/SLC44A1 axis in epithelial ovarian cancer.
Article Snippet: .. Then, the wide-type (Wt) and mutant (Mut) sequences of LINC00704 or SLC44A1 3ʹUTR were amplified and subcloned into pmirGLO vectors (Promega, Madison, WI, USA) to construct LINC00704-Wt/Mut and SLC44A1-Wt/Mut. .. These constructs were then co-transfected with NC mimics, miR-323a-3p mimics, or miR-323a-3p mimics plus pcDNA3.1/LINC00704 into A2780 and SKOV3 cells using Lipofectamine 2000 (Invitrogen).



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Image Search Results


Sponging relationship between BACE1-AS and miR-6805-5p in HCT-8 cells during C. parvum infection. (A) Subcellular location of BACE1-AS in HCT-8 cells. HCT-8 cells were seeded on a coverslip and were infected with C. parvum for 24 h and then applied for FISH analysis of BACE1-AS using probes conjugated with cy3, with 18S localized in the cytoplasm as a control. The nucleus was visualized by DAPI staining. (B) The mRNA levels of miR-6805-5p in HCT-8 cells transfected with pcDNA3.1(+)-BACE1-AS plasmid or si-BACE1-AS following C. parvum infection at 24 hpi by using RT-qPCR. (C) Putative binding site between BACE1-AS and miR-6805-5p predicted by LncBook. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and pmirGLO-BACE1-AS-WT or pmirGLO-BACE1-AS-MUT. Statistical analysis was conducted by using a non-parametric t -test. * P < 0.05, ** P < 0.01. WT, wild type.

Journal: Microbiology Spectrum

Article Title: LncRNA BACE1-AS delays the propagation of Cryptosporidium parvum through regulating cell apoptosis by targeting the miR-6805-5p/IRF3 axis

doi: 10.1128/spectrum.02022-24

Figure Lengend Snippet: Sponging relationship between BACE1-AS and miR-6805-5p in HCT-8 cells during C. parvum infection. (A) Subcellular location of BACE1-AS in HCT-8 cells. HCT-8 cells were seeded on a coverslip and were infected with C. parvum for 24 h and then applied for FISH analysis of BACE1-AS using probes conjugated with cy3, with 18S localized in the cytoplasm as a control. The nucleus was visualized by DAPI staining. (B) The mRNA levels of miR-6805-5p in HCT-8 cells transfected with pcDNA3.1(+)-BACE1-AS plasmid or si-BACE1-AS following C. parvum infection at 24 hpi by using RT-qPCR. (C) Putative binding site between BACE1-AS and miR-6805-5p predicted by LncBook. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and pmirGLO-BACE1-AS-WT or pmirGLO-BACE1-AS-MUT. Statistical analysis was conducted by using a non-parametric t -test. * P < 0.05, ** P < 0.01. WT, wild type.

Article Snippet: In this study, the luciferase reporter plasmids of wild-type BACE1-AS (BACE1-AS -WT), mutant BACE1-AS (BACE1-AS -MUT), wild-type IRF3 ( IRF3 -WT), and mutant IRF3 ( IRF3 -MUT) were constructed by cloning BACE1-AS, the miR-6805-5p binding site-mutated BACE1-AS, 3′ UTR region of IRF3 , and the miR-6805-5p binding site-mutated 3′ UTR region of IRF3 into pmirGLO vectors (Promega, Madison, WI, USA), respectively.

Techniques: Infection, Control, Staining, Transfection, Plasmid Preparation, Quantitative RT-PCR, Binding Assay, Luciferase, Activity Assay

Target relationship between miR-6805-5p and IRF3 in HCT-8 cells following C. parvum infection. The mRNA (A) and protein (B) levels of IRF3 in HCT-8 cells transfected with miR-6805-5p mimics or inhibitor following C. parvum infection at 24 hpi by RT-qPCR and Western blot. ImageJ was used to calculate the protein level normalized to GAPDH by densitometry. (C) Putative binding site between the 3′-UTR of IRF3 and miR-6805-5p predicted by miRWalk. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and pmirGLO- IRF3 -WT or pmirGLO- IRF3 -MUT. Three independent experiments were performed. Statistical analysis was conducted by using a non-parametric t- test. * P < 0.05.

Journal: Microbiology Spectrum

Article Title: LncRNA BACE1-AS delays the propagation of Cryptosporidium parvum through regulating cell apoptosis by targeting the miR-6805-5p/IRF3 axis

doi: 10.1128/spectrum.02022-24

Figure Lengend Snippet: Target relationship between miR-6805-5p and IRF3 in HCT-8 cells following C. parvum infection. The mRNA (A) and protein (B) levels of IRF3 in HCT-8 cells transfected with miR-6805-5p mimics or inhibitor following C. parvum infection at 24 hpi by RT-qPCR and Western blot. ImageJ was used to calculate the protein level normalized to GAPDH by densitometry. (C) Putative binding site between the 3′-UTR of IRF3 and miR-6805-5p predicted by miRWalk. (D) The luciferase activity in HCT-8 cells co-transfected with miR-6805-5p mimics and pmirGLO- IRF3 -WT or pmirGLO- IRF3 -MUT. Three independent experiments were performed. Statistical analysis was conducted by using a non-parametric t- test. * P < 0.05.

Article Snippet: In this study, the luciferase reporter plasmids of wild-type BACE1-AS (BACE1-AS -WT), mutant BACE1-AS (BACE1-AS -MUT), wild-type IRF3 ( IRF3 -WT), and mutant IRF3 ( IRF3 -MUT) were constructed by cloning BACE1-AS, the miR-6805-5p binding site-mutated BACE1-AS, 3′ UTR region of IRF3 , and the miR-6805-5p binding site-mutated 3′ UTR region of IRF3 into pmirGLO vectors (Promega, Madison, WI, USA), respectively.

Techniques: Infection, Transfection, Quantitative RT-PCR, Western Blot, Binding Assay, Luciferase, Activity Assay