pmirglo vectors (wt or mut) (Promega)
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Pmirglo Vectors (Wt Or Mut), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmirglo+vectors/pmirglo+vector/pm40639725-237-1-15
Average 90 stars, based on 1 article reviews
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Mutagenesis:Article Title: tRF ‐34‐ P4R8YP9LON4VHM Promotes Hepatocellular Carcinoma Progression and Tumour Cell‐Induced Angiogenesis via the MEK / ERK Pathway Article Snippet: .. As DAB2IP was the target of tRF‐34‐P4R8YP9LON4VHM, we used the mutant (MUT) and wild type (WT) of the DAB2IP 3′ UTR to treat Article Title: tRF-34-P4R8YP9LON4VHM Promotes Hepatocellular Carcinoma Progression and Tumour Cell-Induced Angiogenesis via the MEK/ERK Pathway. Article Snippet: .. As DAB2IP was the target of tRF- 34- P4R8YP9LON4VHM, we used the mutant (MUT) and wild type (WT) of the DAB2IP 3′ UTR to treat Article Title: LncRNA BACE1-AS delays the propagation of Cryptosporidium parvum through regulating cell apoptosis by targeting the miR-6805-5p/IRF3 axis Article Snippet: .. In this study, the luciferase reporter plasmids of wild-type BACE1-AS (BACE1-AS -WT), mutant BACE1-AS (BACE1-AS -MUT), wild-type IRF3 ( IRF3 -WT), and mutant IRF3 ( IRF3 -MUT) were constructed by cloning BACE1-AS, the miR-6805-5p binding site-mutated BACE1-AS, 3′ UTR region of IRF3 , and the miR-6805-5p binding site-mutated 3′ UTR region of IRF3 into Article Title: LINC00704 facilitates cell proliferation, migration, and invasion via miR-323a-3p/SLC44A1 axis in epithelial ovarian cancer. Article Snippet: .. Then, the wide-type (Wt) and mutant (Mut) sequences of LINC00704 or SLC44A1 3ʹUTR were amplified and subcloned into Article Title: LncRNA XIST enhances gastric cancer cell function by regulating STAT3/PD-L1 axis as a competing endogenous RNA for miR-124 Article Snippet: .. The predicted wild-type (WT) and mutant (Mut) sequences of the XIST binding site for miR-124 were cloned into Article Title: LINC00265 Serves as a Diagnostic and Prognostic Marker for Acute Ischemic Stroke and INHIBITS Disease Progression Through the miR- 155 - 5p/TRIM32 Axis. Article Snippet: Acute ischemic stroke (AIS) is a medical emergency stemming from a reduction in cerebral blood supply, leading to neuronal injury.. LINC00265 may emerge as a highly promising biomarker for stroke.. The aim of this investigation was to investigate the clinical and prognostic significance of LINC00265 in AIS, as well as to elucidate the molecular mechanisms through which LINC00265 influences AIS. Clone Assay:Article Title: Cancer-associated fibroblasts-derived exosomal piR-35462 promotes the progression of oral squamous cell carcinoma via FTO/Twist1 pathway. Article Snippet: Inhibitor, mimic, as well as plasmid construction The cDNA encoding Fat Mass and Obesity-Associated Protein (FTO) was inserted into the pcDNA3.1-Myc/ His vector (Invitrogen, US) and its sequence was confirmed. .. Wild type (WT) FTO 3’-UTR sequences were cloned into Article Title: LncRNA XIST enhances gastric cancer cell function by regulating STAT3/PD-L1 axis as a competing endogenous RNA for miR-124 Article Snippet: .. The predicted wild-type (WT) and mutant (Mut) sequences of the XIST binding site for miR-124 were cloned into Article Title: LINC01088 Targets miR-195-5p to Promote Proliferation and Migration and Reduce Apoptosis in the Inhibition of Carotid Artery Stenosis. Article Snippet: .. The partial sequences of LINC0108 containing the putative binding sites of miR-195-5p cloned into the Article Title: LINC00265 Serves as a Diagnostic and Prognostic Marker for Acute Ischemic Stroke and INHIBITS Disease Progression Through the miR- 155 - 5p/TRIM32 Axis. Article Snippet: Acute ischemic stroke (AIS) is a medical emergency stemming from a reduction in cerebral blood supply, leading to neuronal injury.. LINC00265 may emerge as a highly promising biomarker for stroke.. The aim of this investigation was to investigate the clinical and prognostic significance of LINC00265 in AIS, as well as to elucidate the molecular mechanisms through which LINC00265 influences AIS. Luciferase:Article Title: Cancer-associated fibroblasts-derived exosomal piR-35462 promotes the progression of oral squamous cell carcinoma via FTO/Twist1 pathway. Article Snippet: Inhibitor, mimic, as well as plasmid construction The cDNA encoding Fat Mass and Obesity-Associated Protein (FTO) was inserted into the pcDNA3.1-Myc/ His vector (Invitrogen, US) and its sequence was confirmed. .. Wild type (WT) FTO 3’-UTR sequences were cloned into Article Title: LncRNA BACE1-AS delays the propagation of Cryptosporidium parvum through regulating cell apoptosis by targeting the miR-6805-5p/IRF3 axis Article Snippet: .. In this study, the luciferase reporter plasmids of wild-type BACE1-AS (BACE1-AS -WT), mutant BACE1-AS (BACE1-AS -MUT), wild-type IRF3 ( IRF3 -WT), and mutant IRF3 ( IRF3 -MUT) were constructed by cloning BACE1-AS, the miR-6805-5p binding site-mutated BACE1-AS, 3′ UTR region of IRF3 , and the miR-6805-5p binding site-mutated 3′ UTR region of IRF3 into Construct:Article Title: LncRNA BACE1-AS delays the propagation of Cryptosporidium parvum through regulating cell apoptosis by targeting the miR-6805-5p/IRF3 axis Article Snippet: .. In this study, the luciferase reporter plasmids of wild-type BACE1-AS (BACE1-AS -WT), mutant BACE1-AS (BACE1-AS -MUT), wild-type IRF3 ( IRF3 -WT), and mutant IRF3 ( IRF3 -MUT) were constructed by cloning BACE1-AS, the miR-6805-5p binding site-mutated BACE1-AS, 3′ UTR region of IRF3 , and the miR-6805-5p binding site-mutated 3′ UTR region of IRF3 into Article Title: LINC00704 facilitates cell proliferation, migration, and invasion via miR-323a-3p/SLC44A1 axis in epithelial ovarian cancer. Article Snippet: .. Then, the wide-type (Wt) and mutant (Mut) sequences of LINC00704 or SLC44A1 3ʹUTR were amplified and subcloned into Cloning:Article Title: LncRNA BACE1-AS delays the propagation of Cryptosporidium parvum through regulating cell apoptosis by targeting the miR-6805-5p/IRF3 axis Article Snippet: .. In this study, the luciferase reporter plasmids of wild-type BACE1-AS (BACE1-AS -WT), mutant BACE1-AS (BACE1-AS -MUT), wild-type IRF3 ( IRF3 -WT), and mutant IRF3 ( IRF3 -MUT) were constructed by cloning BACE1-AS, the miR-6805-5p binding site-mutated BACE1-AS, 3′ UTR region of IRF3 , and the miR-6805-5p binding site-mutated 3′ UTR region of IRF3 into Binding Assay:Article Title: LncRNA BACE1-AS delays the propagation of Cryptosporidium parvum through regulating cell apoptosis by targeting the miR-6805-5p/IRF3 axis Article Snippet: .. In this study, the luciferase reporter plasmids of wild-type BACE1-AS (BACE1-AS -WT), mutant BACE1-AS (BACE1-AS -MUT), wild-type IRF3 ( IRF3 -WT), and mutant IRF3 ( IRF3 -MUT) were constructed by cloning BACE1-AS, the miR-6805-5p binding site-mutated BACE1-AS, 3′ UTR region of IRF3 , and the miR-6805-5p binding site-mutated 3′ UTR region of IRF3 into Article Title: LncRNA XIST enhances gastric cancer cell function by regulating STAT3/PD-L1 axis as a competing endogenous RNA for miR-124 Article Snippet: .. The predicted wild-type (WT) and mutant (Mut) sequences of the XIST binding site for miR-124 were cloned into Article Title: LINC01088 Targets miR-195-5p to Promote Proliferation and Migration and Reduce Apoptosis in the Inhibition of Carotid Artery Stenosis. Article Snippet: .. The partial sequences of LINC0108 containing the putative binding sites of miR-195-5p cloned into the Amplification:Article Title: LINC00704 facilitates cell proliferation, migration, and invasion via miR-323a-3p/SLC44A1 axis in epithelial ovarian cancer. Article Snippet: .. Then, the wide-type (Wt) and mutant (Mut) sequences of LINC00704 or SLC44A1 3ʹUTR were amplified and subcloned into |
