pmdl (Addgene inc)
98
Structured Review
Addgene inc
pmdl
Pmdl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 14739 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmdl/pMD2%2EG+(Plasmid+%2312259)/pm41923618-224-13-14
Average 98 stars, based on 14739 article reviews
Pmdl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 14739 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmdl/pMD2%2EG+(Plasmid+%2312259)/pm41923618-224-13-14
Average 98 stars, based on 14739 article reviews
pmdl - by Bioz Stars,
2026-09
98/100 stars
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Transfection:Article Title: Lentiviral Dendritic Cell Vaccine Targeting Claudin-18.2 Elicits Potent Antitumor Immunity Against Gastric Cancer Article Snippet: .. Cells were transfected with pLVX-CLDN18.2 (or empty vector), Article Title: Estrogen Production in Pancreatic Cancer Shapes a Tumor-Suppressive Stromal Microenvironment Article Snippet: The scrambled shc002 (RRID: Addgene_1864) was used as a control. .. Lentivirus was generated by transfecting HEK293T cells (RRID: CVCL_0063) with the MISSION TRC library pLKO.1 plasmid (RRID: Addgene_10878) containing the appropriate TRC clone (mentioned above) and the envelope/packaging plasmids pMD2.G (RRID: Addgene_12259), Article Title: Lentiviral Dendritic Cell Vaccine Targeting Claudin-18.2 Elicits Potent Antitumor Immunity Against Gastric Cancer. Article Snippet: .. Cells were transfected with pLVXCLDN18.2 (or empty vector), Produced:Article Title: Induced Proprioceptor and Low‐Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00‐4555‐56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co‐transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW‐M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg Article Title: Induced Proprioceptor and Low-Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties. Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00-4555-56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co-transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW-M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg Plasmid Preparation:Article Title: Induced Proprioceptor and Low‐Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00‐4555‐56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co‐transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW‐M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg Article Title: Estrogen Production in Pancreatic Cancer Shapes a Tumor-Suppressive Stromal Microenvironment Article Snippet: The scrambled shc002 (RRID: Addgene_1864) was used as a control. .. Lentivirus was generated by transfecting HEK293T cells (RRID: CVCL_0063) with the MISSION TRC library pLKO.1 plasmid (RRID: Addgene_10878) containing the appropriate TRC clone (mentioned above) and the envelope/packaging plasmids pMD2.G (RRID: Addgene_12259), Article Title: Induced Proprioceptor and Low-Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties. Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00-4555-56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co-transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW-M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg Expressing:Article Title: Induced Proprioceptor and Low‐Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00‐4555‐56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co‐transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW‐M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg Article Title: Induced Proprioceptor and Low-Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties. Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00-4555-56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co-transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW-M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg Generated:Article Title: Estrogen Production in Pancreatic Cancer Shapes a Tumor-Suppressive Stromal Microenvironment Article Snippet: The scrambled shc002 (RRID: Addgene_1864) was used as a control. .. Lentivirus was generated by transfecting HEK293T cells (RRID: CVCL_0063) with the MISSION TRC library pLKO.1 plasmid (RRID: Addgene_10878) containing the appropriate TRC clone (mentioned above) and the envelope/packaging plasmids pMD2.G (RRID: Addgene_12259), Article Title: IAP-based biodegraders can convert necroptosis to apoptosis and eliminate cancer driving protein complexes. Article Snippet: .. Lentivirus was generated by transfecting DNA and helper plasmids pMD2.G (Addgene, Cat# 12259), other:Article Title: Protocol for efficient CRISPRi-mediated silencing of retrotransposons in human pluripotent stem cells Article Snippet: pMDL , Addgene; |