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Addgene inc pmdl
Pmdl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 14739 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmdl/pMD2%2EG+(Plasmid+%2312259)/pm41923618-224-13-14
Average 98 stars, based on 14739 article reviews
pmdl - by Bioz Stars, 2026-09
98/100 stars

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Transfection:

Article Title: Lentiviral Dendritic Cell Vaccine Targeting Claudin-18.2 Elicits Potent Antitumor Immunity Against Gastric Cancer
Article Snippet: .. Cells were transfected with pLVX-CLDN18.2 (or empty vector), pMDL (Addgene, 12253), pVSVG and pRSV-Rev using PEI transfection reagent (Yeasen, 40816ES01) (Shanghai, China). .. Supernatants were harvested 72 h later, cleared (500 g , 10 min, 4 °C), filtered (0.45 μm PVDF; Millipore, HAWP04700) (Burlington, MA, USA) and precipitated with 1/4 volume ice-cold 5× PEG-6000/NaCl overnight at 4 °C.

Article Title: Estrogen Production in Pancreatic Cancer Shapes a Tumor-Suppressive Stromal Microenvironment
Article Snippet: The scrambled shc002 (RRID: Addgene_1864) was used as a control. .. Lentivirus was generated by transfecting HEK293T cells (RRID: CVCL_0063) with the MISSION TRC library pLKO.1 plasmid (RRID: Addgene_10878) containing the appropriate TRC clone (mentioned above) and the envelope/packaging plasmids pMD2.G (RRID: Addgene_12259), pMDL (RRID: Addgene_12251), and pRSV-REV (RRID: Addgene_12253) using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific), as described previously ( ). .. The virus-containing supernatant was subsequently harvested (at 48 and 72 hours) and filtered through 0.45-μm filters (Millipore).

Article Title: Lentiviral Dendritic Cell Vaccine Targeting Claudin-18.2 Elicits Potent Antitumor Immunity Against Gastric Cancer.
Article Snippet: .. Cells were transfected with pLVXCLDN18.2 (or empty vector), pMDL (Addgene, 12253), pVSVG and pRSV-Rev using PEI transfection reagent (Yeasen, 40816ES01) (Shanghai, China). .. Supernatants were harvested 72 h later, cleared (500 g, 10 min, 4 ◦C), filtered (0.45 μm PVDF; Millipore, HAWP04700) https://doi.org/10.3390/cancers18030441 (Burlington, MA, USA) and precipitated with 1/4 volume ice-cold 5× PEG-6000/NaCl overnight at 4 ◦C.

Produced:

Article Title: Induced Proprioceptor and Low‐Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties
Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00‐4555‐56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co‐transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW‐M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg pMDL (#12251, Addgene), 3 μg vSVG (#8454, Addgene), and 3 μg RSV (#12253, Addgene), using polyethyleneimine (PEI) (408727, Sigma) at a ratio of 3:1 PEI:DNA in Opti‐MEM (#31985062, Life technologies). ..

Article Title: Induced Proprioceptor and Low-Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties.
Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00-4555-56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co-transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW-M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg pMDL (#12251, Addgene), 3 μg vSVG (#8454, Addgene), and 3 μg RSV (#12253, Addgene), using polyethyleneimine (PEI) (408727, Sigma) at a ratio of 3:1 PEI:DNA in Opti-MEM (#31985062, Life technologies). ..

Plasmid Preparation:

Article Title: Induced Proprioceptor and Low‐Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties
Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00‐4555‐56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co‐transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW‐M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg pMDL (#12251, Addgene), 3 μg vSVG (#8454, Addgene), and 3 μg RSV (#12253, Addgene), using polyethyleneimine (PEI) (408727, Sigma) at a ratio of 3:1 PEI:DNA in Opti‐MEM (#31985062, Life technologies). ..

Article Title: Estrogen Production in Pancreatic Cancer Shapes a Tumor-Suppressive Stromal Microenvironment
Article Snippet: The scrambled shc002 (RRID: Addgene_1864) was used as a control. .. Lentivirus was generated by transfecting HEK293T cells (RRID: CVCL_0063) with the MISSION TRC library pLKO.1 plasmid (RRID: Addgene_10878) containing the appropriate TRC clone (mentioned above) and the envelope/packaging plasmids pMD2.G (RRID: Addgene_12259), pMDL (RRID: Addgene_12251), and pRSV-REV (RRID: Addgene_12253) using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific), as described previously ( ). .. The virus-containing supernatant was subsequently harvested (at 48 and 72 hours) and filtered through 0.45-μm filters (Millipore).

Article Title: Induced Proprioceptor and Low-Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties.
Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00-4555-56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co-transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW-M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg pMDL (#12251, Addgene), 3 μg vSVG (#8454, Addgene), and 3 μg RSV (#12253, Addgene), using polyethyleneimine (PEI) (408727, Sigma) at a ratio of 3:1 PEI:DNA in Opti-MEM (#31985062, Life technologies). ..

Expressing:

Article Title: Induced Proprioceptor and Low‐Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties
Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00‐4555‐56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co‐transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW‐M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg pMDL (#12251, Addgene), 3 μg vSVG (#8454, Addgene), and 3 μg RSV (#12253, Addgene), using polyethyleneimine (PEI) (408727, Sigma) at a ratio of 3:1 PEI:DNA in Opti‐MEM (#31985062, Life technologies). ..

Article Title: Induced Proprioceptor and Low-Threshold Mechanoreceptor Neurons Derived from Human Pluripotent Stem Cells Exhibit Distinct Functional Mechanosensory Properties.
Article Snippet: For lentiviral production, HEK293T cells were passaged with Accutase (#00-4555-56, ThermoFisher) and were seeded at a density of 5000 000 cells/T75 flask. .. Lentiviral particles were produced 24 h after seeding (90–100% confluence) by co-transfecting 12 μg of the plasmid encoding for the expression vector of interest, or 12 μg of the reverse tetracycline transactivator vector FUW-M2rtTA (#20342, Addgene), with the lentiviral packaging plasmids: 6 μg pMDL (#12251, Addgene), 3 μg vSVG (#8454, Addgene), and 3 μg RSV (#12253, Addgene), using polyethyleneimine (PEI) (408727, Sigma) at a ratio of 3:1 PEI:DNA in Opti-MEM (#31985062, Life technologies). ..

Generated:

Article Title: Estrogen Production in Pancreatic Cancer Shapes a Tumor-Suppressive Stromal Microenvironment
Article Snippet: The scrambled shc002 (RRID: Addgene_1864) was used as a control. .. Lentivirus was generated by transfecting HEK293T cells (RRID: CVCL_0063) with the MISSION TRC library pLKO.1 plasmid (RRID: Addgene_10878) containing the appropriate TRC clone (mentioned above) and the envelope/packaging plasmids pMD2.G (RRID: Addgene_12259), pMDL (RRID: Addgene_12251), and pRSV-REV (RRID: Addgene_12253) using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific), as described previously ( ). .. The virus-containing supernatant was subsequently harvested (at 48 and 72 hours) and filtered through 0.45-μm filters (Millipore).

Article Title: IAP-based biodegraders can convert necroptosis to apoptosis and eliminate cancer driving protein complexes.
Article Snippet: .. Lentivirus was generated by transfecting DNA and helper plasmids pMD2.G (Addgene, Cat# 12259), pMDL (Addgene, Cat# 12251) and pRSV-REV (Addgene, Cat# 12253) into HEK293T cells using Lipofectamine 3000 (Thermo Fisher, Cat# L3000008). .. Supernatants containing lentiviral particles were collected at 48 h and 72 h after transfection and filtered through 0.45 μm pore-size filter caps (Sartorius).

other:

Article Title: Protocol for efficient CRISPRi-mediated silencing of retrotransposons in human pluripotent stem cells
Article Snippet: pMDL , Addgene; Zufferey et al . , RRID: Addgene _12251.



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