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pmcherry n1 vector  (TaKaRa)


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    Structured Review

    TaKaRa pmcherry n1 vector
    Pmcherry N1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3394 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmcherry+n1+vector/pmCherry-N1+Vector/10__1038_slash_s41598___026___74621___1-133-18-20
    Average 96 stars, based on 3394 article reviews
    pmcherry n1 vector - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: The relationship between Connexin 43 (Cx43) and partner protein, human discs large homologue-1 (Dlg1) during wound closure in keratinocytes.
    Article Snippet: Cells were detached from plates using 0.05% trypsin–EDTA (Thermo Fisher Scientific, UK, catalogue # 25,300–054) and passaged three times a week at either a 1:5 or a 1:10 dilution depending on growth rate. .. To generate a plasmid containing the sequence of human Cx43 with a C-terminal mCherry tag, we designed primers to clone human Cx43 into the pmCherry-N1 vector (Clontech) (primer sequences shown in Table 1). .. Primers were synthesised by Eurofins, UK, and used to amplify Cx43 from pCR3.1Cx43 using a High Fidelity PCR mix (Roche, UK Catalogue # 03310256103).

    Article Title: The S-depalmitoylase ABHD10 is essential for sperm mitochondrial sheath formation and male fertility.
    Article Snippet: .. Briefly, the endogenous Abhd10 promoter was amplified from mouse genomic DNA and used to replace the CMV promoter in the pmCherry-N1 vector (Clontech, 632523). ..

    Article Title: Divergent regulatory element programs steer sex-specific supporting cell differentiation along mouse gonadal development
    Article Snippet: .. Enh8 (mm9: 111,805,552-111,806,224; 672 bp long, located 838 kb upstream of the Sox9 gene) was amplified by PCR and cloned into the AseI restriction sites of the pmCherry-N1 vector (Kan resistance) reporter vector (Takara Cat No. 632523) using In- Fusion HD (Clontech). .. The hsp68 sequence was amplified from the pSfi-Hsp68-LacZ reporter vector (Addgene #33351) via In- Fusion HD and cloned into the AseI and AgeI restriction sites of the pmCherry-N1 vector.

    Article Title: The S-depalmitoylase ABHD10 is essential for sperm mitochondrial sheath formation and male fertility
    Article Snippet: .. Briefly, the endogenous Abhd10 promoter was amplified from mouse genomic DNA and used to replace the CMV promoter in the pmCherry-N1 vector (Clontech, 632523). ..

    Article Title: Role of calcineurin B homologous protein 3 in C2C12 myogenic differentiation
    Article Snippet: Transient overexpression of CHP3 in C2C12 cells Total RNA was extracted from C2C12 cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and reversetranscribed into cDNA with ReverTra Ace -α- (Toyobo, Osaka, Japan). .. The coding sequence of mouse CHP3 was amplified by PCR and subcloned in-frame upstream of mCherry in the pmCherry-N1 vector (Takara Bio, Shiga, Japan) using the In-Fusion HD Cloning Kit (Takara Bio). ..

    Article Title: The relationship between Connexin 43 (Cx43) and partner protein, human discs large homologue-1 (Dlg1) during wound closure in keratinocytes
    Article Snippet: Cells were detached from plates using 0.05% trypsin–EDTA (Thermo Fisher Scientific, UK, catalogue # 25,300–054) and passaged three times a week at either a 1:5 or a 1:10 dilution depending on growth rate. .. To generate a plasmid containing the sequence of human Cx43 with a C-terminal mCherry tag, we designed primers to clone human Cx43 into the pmCherry-N1 vector (Clontech) (primer sequences shown in Table ). .. Primers were synthesised by Eurofins, UK, and used to amplify Cx43 from pCR3.1Cx43 using a High Fidelity PCR mix (Roche, UK Catalogue # 03310256103).

    Sequencing:

    Article Title: The relationship between Connexin 43 (Cx43) and partner protein, human discs large homologue-1 (Dlg1) during wound closure in keratinocytes.
    Article Snippet: Cells were detached from plates using 0.05% trypsin–EDTA (Thermo Fisher Scientific, UK, catalogue # 25,300–054) and passaged three times a week at either a 1:5 or a 1:10 dilution depending on growth rate. .. To generate a plasmid containing the sequence of human Cx43 with a C-terminal mCherry tag, we designed primers to clone human Cx43 into the pmCherry-N1 vector (Clontech) (primer sequences shown in Table 1). .. Primers were synthesised by Eurofins, UK, and used to amplify Cx43 from pCR3.1Cx43 using a High Fidelity PCR mix (Roche, UK Catalogue # 03310256103).

    Article Title: Role of calcineurin B homologous protein 3 in C2C12 myogenic differentiation
    Article Snippet: Transient overexpression of CHP3 in C2C12 cells Total RNA was extracted from C2C12 cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and reversetranscribed into cDNA with ReverTra Ace -α- (Toyobo, Osaka, Japan). .. The coding sequence of mouse CHP3 was amplified by PCR and subcloned in-frame upstream of mCherry in the pmCherry-N1 vector (Takara Bio, Shiga, Japan) using the In-Fusion HD Cloning Kit (Takara Bio). ..

    Article Title: The relationship between Connexin 43 (Cx43) and partner protein, human discs large homologue-1 (Dlg1) during wound closure in keratinocytes
    Article Snippet: Cells were detached from plates using 0.05% trypsin–EDTA (Thermo Fisher Scientific, UK, catalogue # 25,300–054) and passaged three times a week at either a 1:5 or a 1:10 dilution depending on growth rate. .. To generate a plasmid containing the sequence of human Cx43 with a C-terminal mCherry tag, we designed primers to clone human Cx43 into the pmCherry-N1 vector (Clontech) (primer sequences shown in Table ). .. Primers were synthesised by Eurofins, UK, and used to amplify Cx43 from pCR3.1Cx43 using a High Fidelity PCR mix (Roche, UK Catalogue # 03310256103).

    Amplification:

    Article Title: The S-depalmitoylase ABHD10 is essential for sperm mitochondrial sheath formation and male fertility.
    Article Snippet: .. Briefly, the endogenous Abhd10 promoter was amplified from mouse genomic DNA and used to replace the CMV promoter in the pmCherry-N1 vector (Clontech, 632523). ..

    Article Title: Divergent regulatory element programs steer sex-specific supporting cell differentiation along mouse gonadal development
    Article Snippet: .. Enh8 (mm9: 111,805,552-111,806,224; 672 bp long, located 838 kb upstream of the Sox9 gene) was amplified by PCR and cloned into the AseI restriction sites of the pmCherry-N1 vector (Kan resistance) reporter vector (Takara Cat No. 632523) using In- Fusion HD (Clontech). .. The hsp68 sequence was amplified from the pSfi-Hsp68-LacZ reporter vector (Addgene #33351) via In- Fusion HD and cloned into the AseI and AgeI restriction sites of the pmCherry-N1 vector.

    Article Title: The S-depalmitoylase ABHD10 is essential for sperm mitochondrial sheath formation and male fertility
    Article Snippet: .. Briefly, the endogenous Abhd10 promoter was amplified from mouse genomic DNA and used to replace the CMV promoter in the pmCherry-N1 vector (Clontech, 632523). ..

    Article Title: Role of calcineurin B homologous protein 3 in C2C12 myogenic differentiation
    Article Snippet: Transient overexpression of CHP3 in C2C12 cells Total RNA was extracted from C2C12 cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and reversetranscribed into cDNA with ReverTra Ace -α- (Toyobo, Osaka, Japan). .. The coding sequence of mouse CHP3 was amplified by PCR and subcloned in-frame upstream of mCherry in the pmCherry-N1 vector (Takara Bio, Shiga, Japan) using the In-Fusion HD Cloning Kit (Takara Bio). ..

    Polymerase Chain Reaction:

    Article Title: Divergent regulatory element programs steer sex-specific supporting cell differentiation along mouse gonadal development
    Article Snippet: .. Enh8 (mm9: 111,805,552-111,806,224; 672 bp long, located 838 kb upstream of the Sox9 gene) was amplified by PCR and cloned into the AseI restriction sites of the pmCherry-N1 vector (Kan resistance) reporter vector (Takara Cat No. 632523) using In- Fusion HD (Clontech). .. The hsp68 sequence was amplified from the pSfi-Hsp68-LacZ reporter vector (Addgene #33351) via In- Fusion HD and cloned into the AseI and AgeI restriction sites of the pmCherry-N1 vector.

    Article Title: Role of calcineurin B homologous protein 3 in C2C12 myogenic differentiation
    Article Snippet: Transient overexpression of CHP3 in C2C12 cells Total RNA was extracted from C2C12 cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and reversetranscribed into cDNA with ReverTra Ace -α- (Toyobo, Osaka, Japan). .. The coding sequence of mouse CHP3 was amplified by PCR and subcloned in-frame upstream of mCherry in the pmCherry-N1 vector (Takara Bio, Shiga, Japan) using the In-Fusion HD Cloning Kit (Takara Bio). ..

    Article Title: Sorting nexin 10 regulates lysosomal ionic homeostasis via ClC-7 by controlling PI(3,5)P2.
    Article Snippet: Mutations or ablation of Snx10 are associated with neurodegeneration, blindness, and osteopetrosis.. The similarities between osteoclasts and macrophages prompted us to analyze the role of Snx10 in phagocytosis.. Deletion of Snx10 impaired phagosome resolution.

    Clone Assay:

    Article Title: Divergent regulatory element programs steer sex-specific supporting cell differentiation along mouse gonadal development
    Article Snippet: .. Enh8 (mm9: 111,805,552-111,806,224; 672 bp long, located 838 kb upstream of the Sox9 gene) was amplified by PCR and cloned into the AseI restriction sites of the pmCherry-N1 vector (Kan resistance) reporter vector (Takara Cat No. 632523) using In- Fusion HD (Clontech). .. The hsp68 sequence was amplified from the pSfi-Hsp68-LacZ reporter vector (Addgene #33351) via In- Fusion HD and cloned into the AseI and AgeI restriction sites of the pmCherry-N1 vector.

    Cloning:

    Article Title: Role of calcineurin B homologous protein 3 in C2C12 myogenic differentiation
    Article Snippet: Transient overexpression of CHP3 in C2C12 cells Total RNA was extracted from C2C12 cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and reversetranscribed into cDNA with ReverTra Ace -α- (Toyobo, Osaka, Japan). .. The coding sequence of mouse CHP3 was amplified by PCR and subcloned in-frame upstream of mCherry in the pmCherry-N1 vector (Takara Bio, Shiga, Japan) using the In-Fusion HD Cloning Kit (Takara Bio). ..

    Construct:

    Article Title: Sorting nexin 10 regulates lysosomal ionic homeostasis via ClC-7 by controlling PI(3,5)P2.
    Article Snippet: Mutations or ablation of Snx10 are associated with neurodegeneration, blindness, and osteopetrosis.. The similarities between osteoclasts and macrophages prompted us to analyze the role of Snx10 in phagocytosis.. Deletion of Snx10 impaired phagosome resolution.

    Over Expression:

    Article Title: Apoptotic and proinflammatory caspases orchestrate necrotic cell death by cleaving three gasdermin orthologs in the highly adaptable killifish
    Article Snippet: The coding sequences of killifish GSDMEa/b/c and caspases were synthesized by Sangon Biotech (Shanghai, China). .. For overexpression of GSDMEa/b/c and its truncated proteins, the coding sequences of GSDMEa/b/c, N-terminal, and C-terminal domain were subcloned into the pmCherry-N1 vector (Clontech, Mountain View, CA) with C-terminal mCherry tag. .. Site-directedmutations were performed using the Hieff Mut Site-Directed Mutagenesis Kit (Yeasen, Shanghai, China).



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