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plvx puro  (TaKaRa)


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    Structured Review

    TaKaRa plvx puro
    Plvx Puro, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3516 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plvx/Puromycin/bio_rxiv__64898__2026__04__22__720056-320-24-25
    Average 96 stars, based on 3516 article reviews
    plvx puro - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Peroxisomal lipid metabolism inhibits Pimozide-induced cancer cell death by regulating ATP homeostasis.
    Article Snippet: .. EGFP-PTS1 was inserted into the pLVX (Clontech) vector through the EcoRI and BamHI sites. .. For plasmids with overexpression of GFP-H2B, GFP-H2B was inserted into the pLVX (Clontech) vector through the EcoRI and BamHI sites.

    Article Title: TCRζ-Driven Pre-Signaling Organization of Lck in Rab11 + Endosomes Shapes TCR Activation
    Article Snippet: .. All Lck constructs were cloned in the pLVX-Tight-Puro vector (Clontech Laboratories), between 5’ NotI and 3’ EcoRI restriction sites. .. The lentiviral packaging plasmids pVSVG (Plasmid #8454) and pSPAX2 (Plasmid #12260) were purchased from Addgene.

    Article Title: Targeting PRDX6-dependent localization and function of GPX4 enhances ferroptosis-mediated tumor suppression.
    Article Snippet: In brief Hu et al. discovered that peroxiredoxin 6 (PRDX6) inhibits lipid peroxidation via dual mechanisms: hydrolyzing hydroperoxy-phospholipids into lysophospholipids and facilitating GPX4 membrane translocation by forming disulfide bonds.. Combining PRDX6 inhibitors with ferroptosis inducers enhances ferroptosis, effectively slowing tumor growth.. This finding opens new avenues for targeted cancer therapy.

    Article Title: Peroxisomal lipid metabolism inhibits Pimozide-induced cancer cell death by regulating ATP homeostasis.
    Article Snippet: EGFP-PTS1 was inserted into the pLVX (Clontech) vector through the EcoRI and BamHI sites. .. For plasmids with overexpression of GFP-H2B, GFP-H2B was inserted into the pLVX (Clontech) vector through the EcoRI and BamHI sites. ..

    shRNA:

    Article Title: NAB2-STAT6 drives an EGR1-dependent neuroendocrine program in solitary fibrous tumors
    Article Snippet: Gene blocks containing three fragments of the long isoform of NAB2-STAT6 (exons 1–4 of NAB2 and exons 2–22 of STAT6) with a C-terminal FLAG tag were ordered from GenScript. .. Using Gibson Assembly Master Mix (NEB, cat#E2611), gene blocks were assembled with a digested pLVX-Tight-Puro (ClonTech) or pLENTI-CMV-GFP-Puro (Addgene, cat#17448) vectors. shRNA oligos were ordered from IDT and cloned into digested Tet-pLKO-puro (21915) vector by ligation. ..

    Clone Assay:

    Article Title: NAB2-STAT6 drives an EGR1-dependent neuroendocrine program in solitary fibrous tumors
    Article Snippet: Gene blocks containing three fragments of the long isoform of NAB2-STAT6 (exons 1–4 of NAB2 and exons 2–22 of STAT6) with a C-terminal FLAG tag were ordered from GenScript. .. Using Gibson Assembly Master Mix (NEB, cat#E2611), gene blocks were assembled with a digested pLVX-Tight-Puro (ClonTech) or pLENTI-CMV-GFP-Puro (Addgene, cat#17448) vectors. shRNA oligos were ordered from IDT and cloned into digested Tet-pLKO-puro (21915) vector by ligation. ..

    Article Title: TCRζ-Driven Pre-Signaling Organization of Lck in Rab11 + Endosomes Shapes TCR Activation
    Article Snippet: .. All Lck constructs were cloned in the pLVX-Tight-Puro vector (Clontech Laboratories), between 5’ NotI and 3’ EcoRI restriction sites. .. The lentiviral packaging plasmids pVSVG (Plasmid #8454) and pSPAX2 (Plasmid #12260) were purchased from Addgene.

    Ligation:

    Article Title: NAB2-STAT6 drives an EGR1-dependent neuroendocrine program in solitary fibrous tumors
    Article Snippet: Gene blocks containing three fragments of the long isoform of NAB2-STAT6 (exons 1–4 of NAB2 and exons 2–22 of STAT6) with a C-terminal FLAG tag were ordered from GenScript. .. Using Gibson Assembly Master Mix (NEB, cat#E2611), gene blocks were assembled with a digested pLVX-Tight-Puro (ClonTech) or pLENTI-CMV-GFP-Puro (Addgene, cat#17448) vectors. shRNA oligos were ordered from IDT and cloned into digested Tet-pLKO-puro (21915) vector by ligation. ..

    Construct:

    Article Title: TCRζ-Driven Pre-Signaling Organization of Lck in Rab11 + Endosomes Shapes TCR Activation
    Article Snippet: .. All Lck constructs were cloned in the pLVX-Tight-Puro vector (Clontech Laboratories), between 5’ NotI and 3’ EcoRI restriction sites. .. The lentiviral packaging plasmids pVSVG (Plasmid #8454) and pSPAX2 (Plasmid #12260) were purchased from Addgene.

    Software:

    Article Title: Targeting PRDX6-dependent localization and function of GPX4 enhances ferroptosis-mediated tumor suppression.
    Article Snippet: In brief Hu et al. discovered that peroxiredoxin 6 (PRDX6) inhibits lipid peroxidation via dual mechanisms: hydrolyzing hydroperoxy-phospholipids into lysophospholipids and facilitating GPX4 membrane translocation by forming disulfide bonds.. Combining PRDX6 inhibitors with ferroptosis inducers enhances ferroptosis, effectively slowing tumor growth.. This finding opens new avenues for targeted cancer therapy.

    Over Expression:

    Article Title: Peroxisomal lipid metabolism inhibits Pimozide-induced cancer cell death by regulating ATP homeostasis.
    Article Snippet: EGFP-PTS1 was inserted into the pLVX (Clontech) vector through the EcoRI and BamHI sites. .. For plasmids with overexpression of GFP-H2B, GFP-H2B was inserted into the pLVX (Clontech) vector through the EcoRI and BamHI sites. ..

    Virus:

    Article Title: Allogeneic CD19 CAR T cells armed with an anti-rejection CD70 CAR overcome antigen escape and evade alloimmune responses.
    Article Snippet: .. Construction of lentiviral and adeno-associated virus vectors encoding anti-CD70 and anti-CD19 CARs The pLVX (Takara Bio, 632164) lentiviral vector (LVV) was employed for cloning and expression of the CD70 CAR or CD70 CAR variants. .. An adeno-associated virus (AAV) plasmid (Cell biolabs, Inc., VPK-411) containing two inverted terminal repeats was modified to incorporate two homology arms, each 300-400 bp in length corresponding to the genomic integration site at the TRAC locus, which flank the nuclease target site.

    Cloning:

    Article Title: Allogeneic CD19 CAR T cells armed with an anti-rejection CD70 CAR overcome antigen escape and evade alloimmune responses.
    Article Snippet: .. Construction of lentiviral and adeno-associated virus vectors encoding anti-CD70 and anti-CD19 CARs The pLVX (Takara Bio, 632164) lentiviral vector (LVV) was employed for cloning and expression of the CD70 CAR or CD70 CAR variants. .. An adeno-associated virus (AAV) plasmid (Cell biolabs, Inc., VPK-411) containing two inverted terminal repeats was modified to incorporate two homology arms, each 300-400 bp in length corresponding to the genomic integration site at the TRAC locus, which flank the nuclease target site.

    Expressing:

    Article Title: Allogeneic CD19 CAR T cells armed with an anti-rejection CD70 CAR overcome antigen escape and evade alloimmune responses.
    Article Snippet: .. Construction of lentiviral and adeno-associated virus vectors encoding anti-CD70 and anti-CD19 CARs The pLVX (Takara Bio, 632164) lentiviral vector (LVV) was employed for cloning and expression of the CD70 CAR or CD70 CAR variants. .. An adeno-associated virus (AAV) plasmid (Cell biolabs, Inc., VPK-411) containing two inverted terminal repeats was modified to incorporate two homology arms, each 300-400 bp in length corresponding to the genomic integration site at the TRAC locus, which flank the nuclease target site.

    Recombinant:

    Article Title: NAB2-STAT6 drives an EGR1-dependent neuroendocrine program in solitary fibrous tumors
    Article Snippet: Sequence-based reagent , INTS10-R , This paper , PCR primers , CGTCTTCCTTTATCCATCTGCC. .. Recombinant DNA reagent , pLVX-Tight-Puro , Clontech , , . .. Recombinant DNA reagent , pLVX-Tet-On Advanced , Clontech , , .



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