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plustiptracker matlab software package  (MathWorks Inc)


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    MathWorks Inc plustiptracker matlab software package
    Kif18B knockdown causes an increase in the lifetime of astral MTs and a decrease in MT growth rate in general. (A, B) First frame of EB1-GFP image series of spindles in PtK1 cells transfected with (A) control or (B) Kif18B-2 siRNA. Scale bar, 10 μm. (C, D) The <t>plusTipTracker</t> software was used to detect and link EB1 comets from time-lapse images of spindles in A and B. Tracked comets were divided into three subspindle regions and color coded accordingly: astral MTs to the left and right of spindle poles (aster_lr) are in magenta, astral MTs in the top and bottom region of the spindle body (aster_tb) are in green, and MTs in the central region of the spindle body are in cyan (Spindle_midzone). (E) Lifetime of EB1 tracks in the three subspindle regions was fit with a one-phase exponential decay to determine the rate constant K (s −1 ), and the average lifetime (τ) was determined from K −1 . Data represent mean ± 95% confidence interval from the fit (see also Supplemental Figure S2) for 11 spindles for the control with 1045 tracks in aster_lr region, 1063 tracks in aster_tb region, and 4030 tracks in spindle_midzone region and 16 Kif18B RNAi spindles with 3605 tracks in aster_lr region, 2295 tracks in aster_tb region, and 7828 tracks in spindle_midzone region. (F) Histograms of EB1 track velocity in the entire spindle of control and Kif18B knockdowns graphed with the fitting curve of a Gaussian distribution. The mean and SD derived from each fit is indicated. Control RNAi, 11 spindles with 7782 tracks; Kif18B RNAi, 16 spindles with 16414 tracks.
    Plustiptracker Matlab Software Package, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plustiptracker+matlab+software+package/pmc05063611-199-10-11
    Average 90 stars, based on 1 article reviews
    plustiptracker matlab software package - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells"

    Article Title: Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells

    Journal: Molecular Biology of the Cell

    doi: 10.1091/mbc.E16-04-0254

    Kif18B knockdown causes an increase in the lifetime of astral MTs and a decrease in MT growth rate in general. (A, B) First frame of EB1-GFP image series of spindles in PtK1 cells transfected with (A) control or (B) Kif18B-2 siRNA. Scale bar, 10 μm. (C, D) The plusTipTracker software was used to detect and link EB1 comets from time-lapse images of spindles in A and B. Tracked comets were divided into three subspindle regions and color coded accordingly: astral MTs to the left and right of spindle poles (aster_lr) are in magenta, astral MTs in the top and bottom region of the spindle body (aster_tb) are in green, and MTs in the central region of the spindle body are in cyan (Spindle_midzone). (E) Lifetime of EB1 tracks in the three subspindle regions was fit with a one-phase exponential decay to determine the rate constant K (s −1 ), and the average lifetime (τ) was determined from K −1 . Data represent mean ± 95% confidence interval from the fit (see also Supplemental Figure S2) for 11 spindles for the control with 1045 tracks in aster_lr region, 1063 tracks in aster_tb region, and 4030 tracks in spindle_midzone region and 16 Kif18B RNAi spindles with 3605 tracks in aster_lr region, 2295 tracks in aster_tb region, and 7828 tracks in spindle_midzone region. (F) Histograms of EB1 track velocity in the entire spindle of control and Kif18B knockdowns graphed with the fitting curve of a Gaussian distribution. The mean and SD derived from each fit is indicated. Control RNAi, 11 spindles with 7782 tracks; Kif18B RNAi, 16 spindles with 16414 tracks.
    Figure Legend Snippet: Kif18B knockdown causes an increase in the lifetime of astral MTs and a decrease in MT growth rate in general. (A, B) First frame of EB1-GFP image series of spindles in PtK1 cells transfected with (A) control or (B) Kif18B-2 siRNA. Scale bar, 10 μm. (C, D) The plusTipTracker software was used to detect and link EB1 comets from time-lapse images of spindles in A and B. Tracked comets were divided into three subspindle regions and color coded accordingly: astral MTs to the left and right of spindle poles (aster_lr) are in magenta, astral MTs in the top and bottom region of the spindle body (aster_tb) are in green, and MTs in the central region of the spindle body are in cyan (Spindle_midzone). (E) Lifetime of EB1 tracks in the three subspindle regions was fit with a one-phase exponential decay to determine the rate constant K (s −1 ), and the average lifetime (τ) was determined from K −1 . Data represent mean ± 95% confidence interval from the fit (see also Supplemental Figure S2) for 11 spindles for the control with 1045 tracks in aster_lr region, 1063 tracks in aster_tb region, and 4030 tracks in spindle_midzone region and 16 Kif18B RNAi spindles with 3605 tracks in aster_lr region, 2295 tracks in aster_tb region, and 7828 tracks in spindle_midzone region. (F) Histograms of EB1 track velocity in the entire spindle of control and Kif18B knockdowns graphed with the fitting curve of a Gaussian distribution. The mean and SD derived from each fit is indicated. Control RNAi, 11 spindles with 7782 tracks; Kif18B RNAi, 16 spindles with 16414 tracks.

    Techniques Used: Knockdown, Transfection, Control, Software, Derivative Assay

    Related Articles

    Knockdown:

    Article Title: Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells
    Article Snippet: microscope was controlled using Nikon Elements software. .. EB1 comets were automatically detected and frame-to-frame linked using the plusTipTracker MATLAB software package (MathWorks, Natick, MA; Applegate et al. , 2011 ). .. A region of interest was drawn along the cell boundary to ex

    Transfection:

    Article Title: Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells
    Article Snippet: microscope was controlled using Nikon Elements software. .. EB1 comets were automatically detected and frame-to-frame linked using the plusTipTracker MATLAB software package (MathWorks, Natick, MA; Applegate et al. , 2011 ). .. A region of interest was drawn along the cell boundary to ex

    Control:

    Article Title: Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells
    Article Snippet: microscope was controlled using Nikon Elements software. .. EB1 comets were automatically detected and frame-to-frame linked using the plusTipTracker MATLAB software package (MathWorks, Natick, MA; Applegate et al. , 2011 ). .. A region of interest was drawn along the cell boundary to ex

    Software:

    Article Title: Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells
    Article Snippet: microscope was controlled using Nikon Elements software. .. EB1 comets were automatically detected and frame-to-frame linked using the plusTipTracker MATLAB software package (MathWorks, Natick, MA; Applegate et al. , 2011 ). .. A region of interest was drawn along the cell boundary to ex

    Derivative Assay:

    Article Title: Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells
    Article Snippet: microscope was controlled using Nikon Elements software. .. EB1 comets were automatically detected and frame-to-frame linked using the plusTipTracker MATLAB software package (MathWorks, Natick, MA; Applegate et al. , 2011 ). .. A region of interest was drawn along the cell boundary to ex



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    plustiptracker matlab software package  (MathWorks Inc)
    90
    MathWorks Inc plustiptracker matlab software package
    Kif18B knockdown causes an increase in the lifetime of astral MTs and a decrease in MT growth rate in general. (A, B) First frame of EB1-GFP image series of spindles in PtK1 cells transfected with (A) control or (B) Kif18B-2 siRNA. Scale bar, 10 μm. (C, D) The <t>plusTipTracker</t> software was used to detect and link EB1 comets from time-lapse images of spindles in A and B. Tracked comets were divided into three subspindle regions and color coded accordingly: astral MTs to the left and right of spindle poles (aster_lr) are in magenta, astral MTs in the top and bottom region of the spindle body (aster_tb) are in green, and MTs in the central region of the spindle body are in cyan (Spindle_midzone). (E) Lifetime of EB1 tracks in the three subspindle regions was fit with a one-phase exponential decay to determine the rate constant K (s −1 ), and the average lifetime (τ) was determined from K −1 . Data represent mean ± 95% confidence interval from the fit (see also Supplemental Figure S2) for 11 spindles for the control with 1045 tracks in aster_lr region, 1063 tracks in aster_tb region, and 4030 tracks in spindle_midzone region and 16 Kif18B RNAi spindles with 3605 tracks in aster_lr region, 2295 tracks in aster_tb region, and 7828 tracks in spindle_midzone region. (F) Histograms of EB1 track velocity in the entire spindle of control and Kif18B knockdowns graphed with the fitting curve of a Gaussian distribution. The mean and SD derived from each fit is indicated. Control RNAi, 11 spindles with 7782 tracks; Kif18B RNAi, 16 spindles with 16414 tracks.
    Plustiptracker Matlab Software Package, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plustiptracker+matlab+software+package/pmc05063611-199-10-11
    Average 90 stars, based on 1 article reviews
    plustiptracker matlab software package - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Kif18B knockdown causes an increase in the lifetime of astral MTs and a decrease in MT growth rate in general. (A, B) First frame of EB1-GFP image series of spindles in PtK1 cells transfected with (A) control or (B) Kif18B-2 siRNA. Scale bar, 10 μm. (C, D) The plusTipTracker software was used to detect and link EB1 comets from time-lapse images of spindles in A and B. Tracked comets were divided into three subspindle regions and color coded accordingly: astral MTs to the left and right of spindle poles (aster_lr) are in magenta, astral MTs in the top and bottom region of the spindle body (aster_tb) are in green, and MTs in the central region of the spindle body are in cyan (Spindle_midzone). (E) Lifetime of EB1 tracks in the three subspindle regions was fit with a one-phase exponential decay to determine the rate constant K (s −1 ), and the average lifetime (τ) was determined from K −1 . Data represent mean ± 95% confidence interval from the fit (see also Supplemental Figure S2) for 11 spindles for the control with 1045 tracks in aster_lr region, 1063 tracks in aster_tb region, and 4030 tracks in spindle_midzone region and 16 Kif18B RNAi spindles with 3605 tracks in aster_lr region, 2295 tracks in aster_tb region, and 7828 tracks in spindle_midzone region. (F) Histograms of EB1 track velocity in the entire spindle of control and Kif18B knockdowns graphed with the fitting curve of a Gaussian distribution. The mean and SD derived from each fit is indicated. Control RNAi, 11 spindles with 7782 tracks; Kif18B RNAi, 16 spindles with 16414 tracks.

    Journal: Molecular Biology of the Cell

    Article Title: Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells

    doi: 10.1091/mbc.E16-04-0254

    Figure Lengend Snippet: Kif18B knockdown causes an increase in the lifetime of astral MTs and a decrease in MT growth rate in general. (A, B) First frame of EB1-GFP image series of spindles in PtK1 cells transfected with (A) control or (B) Kif18B-2 siRNA. Scale bar, 10 μm. (C, D) The plusTipTracker software was used to detect and link EB1 comets from time-lapse images of spindles in A and B. Tracked comets were divided into three subspindle regions and color coded accordingly: astral MTs to the left and right of spindle poles (aster_lr) are in magenta, astral MTs in the top and bottom region of the spindle body (aster_tb) are in green, and MTs in the central region of the spindle body are in cyan (Spindle_midzone). (E) Lifetime of EB1 tracks in the three subspindle regions was fit with a one-phase exponential decay to determine the rate constant K (s −1 ), and the average lifetime (τ) was determined from K −1 . Data represent mean ± 95% confidence interval from the fit (see also Supplemental Figure S2) for 11 spindles for the control with 1045 tracks in aster_lr region, 1063 tracks in aster_tb region, and 4030 tracks in spindle_midzone region and 16 Kif18B RNAi spindles with 3605 tracks in aster_lr region, 2295 tracks in aster_tb region, and 7828 tracks in spindle_midzone region. (F) Histograms of EB1 track velocity in the entire spindle of control and Kif18B knockdowns graphed with the fitting curve of a Gaussian distribution. The mean and SD derived from each fit is indicated. Control RNAi, 11 spindles with 7782 tracks; Kif18B RNAi, 16 spindles with 16414 tracks.

    Article Snippet: EB1 comets were automatically detected and frame-to-frame linked using the plusTipTracker MATLAB software package (MathWorks, Natick, MA; Applegate et al. , 2011 ).

    Techniques: Knockdown, Transfection, Control, Software, Derivative Assay