pml iv (Addgene inc)
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Pml Iv, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plpc+flag/pLPC-Flag-PML-IV+(Plasmid+%2362804)/pm41712379-352-4-5
Average 93 stars, based on 6 article reviews
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Construct:Article Title: Transcriptional regulation of telomeric repeat-containing RNA by the G-quadruplex-binding Ewing sarcoma protein. Article Snippet: We cloned EWS cDNA into pGEX6P-1 (Cytiva, Tokyo, JP, 27-1540-01#) between EcoRI and XhoI to express an N-terminal GST fusion protein (pGEX6P-1-EWS) using EWS forward and reverse primers36,37. pGEX6P-1-RGG3 was constructed by PCR using pGEX6P-1-EWS as a template and contained amino acids 545–656 of EWS cloned into pGEX6P-1 using RGG3 forward and reverse primers. .. Flag-tagged EWS (1–656; Flag-EWS) and EWS lacking the N-terminal region (287–656; Flag-ΔN-term) were constructed by PCR using pGEX6P-1-EWS as the template, Article Title: Transcriptional regulation of telomeric repeat-containing RNA by the G-quadruplex-binding Ewing sarcoma protein Article Snippet: We cloned EWS cDNA into pGEX6P-1 (Cytiva, Tokyo, JP, 27-1540-01#) between Eco RI and Xho I to express an N-terminal GST fusion protein (pGEX6P-1-EWS) using EWS forward and reverse primers , . pGEX6P-1-RGG3 was constructed by PCR using pGEX6P-1-EWS as a template and contained amino acids 545–656 of EWS cloned into pGEX6P-1 using RGG3 forward and reverse primers. .. Flag-tagged EWS (1–656; Flag-EWS) and EWS lacking the N-terminal region (287–656; Flag-ΔN-term) were constructed by PCR using pGEX6P-1-EWS as the template, Polymerase Chain Reaction:Article Title: Transcriptional regulation of telomeric repeat-containing RNA by the G-quadruplex-binding Ewing sarcoma protein. Article Snippet: We cloned EWS cDNA into pGEX6P-1 (Cytiva, Tokyo, JP, 27-1540-01#) between EcoRI and XhoI to express an N-terminal GST fusion protein (pGEX6P-1-EWS) using EWS forward and reverse primers36,37. pGEX6P-1-RGG3 was constructed by PCR using pGEX6P-1-EWS as a template and contained amino acids 545–656 of EWS cloned into pGEX6P-1 using RGG3 forward and reverse primers. .. Flag-tagged EWS (1–656; Flag-EWS) and EWS lacking the N-terminal region (287–656; Flag-ΔN-term) were constructed by PCR using pGEX6P-1-EWS as the template, Article Title: Transcriptional regulation of telomeric repeat-containing RNA by the G-quadruplex-binding Ewing sarcoma protein Article Snippet: We cloned EWS cDNA into pGEX6P-1 (Cytiva, Tokyo, JP, 27-1540-01#) between Eco RI and Xho I to express an N-terminal GST fusion protein (pGEX6P-1-EWS) using EWS forward and reverse primers , . pGEX6P-1-RGG3 was constructed by PCR using pGEX6P-1-EWS as a template and contained amino acids 545–656 of EWS cloned into pGEX6P-1 using RGG3 forward and reverse primers. .. Flag-tagged EWS (1–656; Flag-EWS) and EWS lacking the N-terminal region (287–656; Flag-ΔN-term) were constructed by PCR using pGEX6P-1-EWS as the template, Plasmid Preparation:Article Title: Transcriptional regulation of telomeric repeat-containing RNA by the G-quadruplex-binding Ewing sarcoma protein. Article Snippet: We cloned EWS cDNA into pGEX6P-1 (Cytiva, Tokyo, JP, 27-1540-01#) between EcoRI and XhoI to express an N-terminal GST fusion protein (pGEX6P-1-EWS) using EWS forward and reverse primers36,37. pGEX6P-1-RGG3 was constructed by PCR using pGEX6P-1-EWS as a template and contained amino acids 545–656 of EWS cloned into pGEX6P-1 using RGG3 forward and reverse primers. .. Flag-tagged EWS (1–656; Flag-EWS) and EWS lacking the N-terminal region (287–656; Flag-ΔN-term) were constructed by PCR using pGEX6P-1-EWS as the template, Article Title: Progerin induces a phenotypic switch in vascular smooth muscle cells and triggers replication stress and an aging-associated secretory signature Article Snippet: After selection, cells were sorted using EDTA, to ensure that the cells are in a single cell suspension, and filtering using 1/2–1/3 the diameter to sort them using BD FACSCanto II. .. AoSMC-Flag/Flag-progerin: Retroviral particles containing Article Title: Transcriptional regulation of telomeric repeat-containing RNA by the G-quadruplex-binding Ewing sarcoma protein Article Snippet: We cloned EWS cDNA into pGEX6P-1 (Cytiva, Tokyo, JP, 27-1540-01#) between Eco RI and Xho I to express an N-terminal GST fusion protein (pGEX6P-1-EWS) using EWS forward and reverse primers , . pGEX6P-1-RGG3 was constructed by PCR using pGEX6P-1-EWS as a template and contained amino acids 545–656 of EWS cloned into pGEX6P-1 using RGG3 forward and reverse primers. .. Flag-tagged EWS (1–656; Flag-EWS) and EWS lacking the N-terminal region (287–656; Flag-ΔN-term) were constructed by PCR using pGEX6P-1-EWS as the template, Retroviral:Article Title: Progerin induces a phenotypic switch in vascular smooth muscle cells and triggers replication stress and an aging-associated secretory signature Article Snippet: After selection, cells were sorted using EDTA, to ensure that the cells are in a single cell suspension, and filtering using 1/2–1/3 the diameter to sort them using BD FACSCanto II. .. AoSMC-Flag/Flag-progerin: Retroviral particles containing Infection:Article Title: Progerin induces a phenotypic switch in vascular smooth muscle cells and triggers replication stress and an aging-associated secretory signature Article Snippet: After selection, cells were sorted using EDTA, to ensure that the cells are in a single cell suspension, and filtering using 1/2–1/3 the diameter to sort them using BD FACSCanto II. .. AoSMC-Flag/Flag-progerin: Retroviral particles containing |
