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sh trc  (Addgene inc)


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    Structured Review

    Addgene inc sh trc
    Sh Trc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 378 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+control/pLKO%2E1+-+TRC+control+(Plasmid+%2310879)/pm41862009-117-2-3
    Average 96 stars, based on 378 article reviews
    sh trc - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    shRNA:

    Article Title: Niche-derived laminin-511 promotes midbrain dopaminergic neuron survival and differentiation through YAP.
    Article Snippet: Parkinson’s disease (PD) is a neurodegenerative disorder in which the loss of dopaminergic neurons in the midbrain (mDA neurons) causes progressive loss of motor control and function.. Using embryonic and mDA neurons, midbrain tissue from mice, and differentiated human neural stem cells, we investigated the mechanisms controlling the survival ofmDAneurons.We found that the extracellularmatrix protein laminin-511 (LM511)promoted the survival and differentiation of mDA neurons.. LM511 bound to integrin a3b1 and activated the transcriptional cofactor YAP.

    Clone Assay:

    Article Title: Niche-derived laminin-511 promotes midbrain dopaminergic neuron survival and differentiation through YAP.
    Article Snippet: Parkinson’s disease (PD) is a neurodegenerative disorder in which the loss of dopaminergic neurons in the midbrain (mDA neurons) causes progressive loss of motor control and function.. Using embryonic and mDA neurons, midbrain tissue from mice, and differentiated human neural stem cells, we investigated the mechanisms controlling the survival ofmDAneurons.We found that the extracellularmatrix protein laminin-511 (LM511)promoted the survival and differentiation of mDA neurons.. LM511 bound to integrin a3b1 and activated the transcriptional cofactor YAP.

    Cloning:

    Article Title: Niche-derived laminin-511 promotes midbrain dopaminergic neuron survival and differentiation through YAP.
    Article Snippet: Parkinson’s disease (PD) is a neurodegenerative disorder in which the loss of dopaminergic neurons in the midbrain (mDA neurons) causes progressive loss of motor control and function.. Using embryonic and mDA neurons, midbrain tissue from mice, and differentiated human neural stem cells, we investigated the mechanisms controlling the survival ofmDAneurons.We found that the extracellularmatrix protein laminin-511 (LM511)promoted the survival and differentiation of mDA neurons.. LM511 bound to integrin a3b1 and activated the transcriptional cofactor YAP.

    Plasmid Preparation:

    Article Title: Niche-derived laminin-511 promotes midbrain dopaminergic neuron survival and differentiation through YAP.
    Article Snippet: Parkinson’s disease (PD) is a neurodegenerative disorder in which the loss of dopaminergic neurons in the midbrain (mDA neurons) causes progressive loss of motor control and function.. Using embryonic and mDA neurons, midbrain tissue from mice, and differentiated human neural stem cells, we investigated the mechanisms controlling the survival ofmDAneurons.We found that the extracellularmatrix protein laminin-511 (LM511)promoted the survival and differentiation of mDA neurons.. LM511 bound to integrin a3b1 and activated the transcriptional cofactor YAP.

    Article Title: Combination of galectin inhibitor GCS-100 and BH3 mimetics eliminates both p53 wild type and p53 null AML cells
    Article Snippet: .. As negative control we used pLKO.1 control (plasmid 10879, Addgene, Cambridge, MA, USA). .. As negative control we used pLKO.1 control (plasmid 10879, Addgene, Cambridge, MA, USA).

    Article Title: LGALS3 is connected to CD74 in a previously unknown protein network that is associated with poor survival in patients with AML
    Article Snippet: .. LGALS3 clone TRCN0000029308 targeting residues 606–626 on RefSeq NM_002306.3 was used. pLKO.1 control (plasmid 10879, Addgene, Cambridge, MA, USA) was used as negative control. .. pLKO.1 control (plasmid 10879, Addgene, Cambridge, MA, USA) was used as negative control.

    Article Title: CIP2A regulates cell proliferation via the AKT signaling pathway in human lung cancer
    Article Snippet: .. Lentivirus was produced by co-transfection of pLKO.1 control (ligated with scramble sequence) or other pLKO.1-derived vector with pMD2.G and pCMV-VSVG (both from Addgene, Cambridge, MA, USA) into HEK293T packaging cell lines. ..

    Control:

    Article Title: Niche-derived laminin-511 promotes midbrain dopaminergic neuron survival and differentiation through YAP.
    Article Snippet: Parkinson’s disease (PD) is a neurodegenerative disorder in which the loss of dopaminergic neurons in the midbrain (mDA neurons) causes progressive loss of motor control and function.. Using embryonic and mDA neurons, midbrain tissue from mice, and differentiated human neural stem cells, we investigated the mechanisms controlling the survival ofmDAneurons.We found that the extracellularmatrix protein laminin-511 (LM511)promoted the survival and differentiation of mDA neurons.. LM511 bound to integrin a3b1 and activated the transcriptional cofactor YAP.

    Article Title: CIP2A regulates cancer metabolism and CREB phosphorylation in non-small cell lung cancer
    Article Snippet: .. Lentivirus was produced by co-transfection of pLKO.1 control (ligated with scramble sequence) or other pLKO.1-derived vector with pMD2.G (Addgene, Cambridge, MA) and pCMV-VSVG (Addgene, Cambridge, MA) into HEK293T packaging cell lines. ..

    Article Title: Combination of galectin inhibitor GCS-100 and BH3 mimetics eliminates both p53 wild type and p53 null AML cells
    Article Snippet: .. As negative control we used pLKO.1 control (plasmid 10879, Addgene, Cambridge, MA, USA). .. As negative control we used pLKO.1 control (plasmid 10879, Addgene, Cambridge, MA, USA).

    Article Title: CIP2A regulates cancer metabolism and CREB phosphorylation in non-small cell lung cancer.
    Article Snippet: .. Lentivirus was produced by co-transfection of pLKO.1 control (ligated with scramble sequence) or other pLKO.1-derived vector with pMD2.G (Addgene, Cambridge, MA) and pCMV-VSVG (Addgene, Cambridge, MA) into HEK293T packaging cell lines. ..

    Article Title: LGALS3 is connected to CD74 in a previously unknown protein network that is associated with poor survival in patients with AML
    Article Snippet: .. LGALS3 clone TRCN0000029308 targeting residues 606–626 on RefSeq NM_002306.3 was used. pLKO.1 control (plasmid 10879, Addgene, Cambridge, MA, USA) was used as negative control. .. pLKO.1 control (plasmid 10879, Addgene, Cambridge, MA, USA) was used as negative control.

    Article Title: CIP2A regulates cell proliferation via the AKT signaling pathway in human lung cancer
    Article Snippet: .. Lentivirus was produced by co-transfection of pLKO.1 control (ligated with scramble sequence) or other pLKO.1-derived vector with pMD2.G and pCMV-VSVG (both from Addgene, Cambridge, MA, USA) into HEK293T packaging cell lines. ..

    Produced:

    Article Title: CIP2A regulates cancer metabolism and CREB phosphorylation in non-small cell lung cancer
    Article Snippet: .. Lentivirus was produced by co-transfection of pLKO.1 control (ligated with scramble sequence) or other pLKO.1-derived vector with pMD2.G (Addgene, Cambridge, MA) and pCMV-VSVG (Addgene, Cambridge, MA) into HEK293T packaging cell lines. ..

    Article Title: CIP2A regulates cancer metabolism and CREB phosphorylation in non-small cell lung cancer.
    Article Snippet: .. Lentivirus was produced by co-transfection of pLKO.1 control (ligated with scramble sequence) or other pLKO.1-derived vector with pMD2.G (Addgene, Cambridge, MA) and pCMV-VSVG (Addgene, Cambridge, MA) into HEK293T packaging cell lines. ..

    Article Title: CIP2A regulates cell proliferation via the AKT signaling pathway in human lung cancer
    Article Snippet: .. Lentivirus was produced by co-transfection of pLKO.1 control (ligated with scramble sequence) or other pLKO.1-derived vector with pMD2.G and pCMV-VSVG (both from Addgene, Cambridge, MA, USA) into HEK293T packaging cell lines. ..

    Sequencing:

    Article Title: CIP2A regulates cancer metabolism and CREB phosphorylation in non-small cell lung cancer
    Article Snippet: .. Lentivirus was produced by co-transfection of pLKO.1 control (ligated with scramble sequence) or other pLKO.1-derived vector with pMD2.G (Addgene, Cambridge, MA) and pCMV-VSVG (Addgene, Cambridge, MA) into HEK293T packaging cell lines. ..

    Article Title: CIP2A regulates cancer metabolism and CREB phosphorylation in non-small cell lung cancer.
    Article Snippet: .. Lentivirus was produced by co-transfection of pLKO.1 control (ligated with scramble sequence) or other pLKO.1-derived vector with pMD2.G (Addgene, Cambridge, MA) and pCMV-VSVG (Addgene, Cambridge, MA) into HEK293T packaging cell lines. ..

    Article Title: CIP2A regulates cell proliferation via the AKT signaling pathway in human lung cancer
    Article Snippet: .. Lentivirus was produced by co-transfection of pLKO.1 control (ligated with scramble sequence) or other pLKO.1-derived vector with pMD2.G and pCMV-VSVG (both from Addgene, Cambridge, MA, USA) into HEK293T packaging cell lines. ..

    Negative Control:

    Article Title: Combination of galectin inhibitor GCS-100 and BH3 mimetics eliminates both p53 wild type and p53 null AML cells
    Article Snippet: .. As negative control we used pLKO.1 control (plasmid 10879, Addgene, Cambridge, MA, USA). .. As negative control we used pLKO.1 control (plasmid 10879, Addgene, Cambridge, MA, USA).

    Article Title: LGALS3 is connected to CD74 in a previously unknown protein network that is associated with poor survival in patients with AML
    Article Snippet: .. LGALS3 clone TRCN0000029308 targeting residues 606–626 on RefSeq NM_002306.3 was used. pLKO.1 control (plasmid 10879, Addgene, Cambridge, MA, USA) was used as negative control. .. pLKO.1 control (plasmid 10879, Addgene, Cambridge, MA, USA) was used as negative control.

    other:

    Article Title: CIP2A regulates cancer metabolism and CREB phosphorylation in non-small cell lung cancer
    Article Snippet: Five CIP2A short hairpin RNAs, ligated in pLKO.1 vector, were obtained from Open Biosystems (Open Biosystems, Huntsville, AL).



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    Image Search Results


    Pathological TDP-43 elicits glycolytic impairment in cells. a , b Agilent seahorse glycolytic stress test was performed on n = 3 independent biological repeats. Glycolysis and Glycolytic Capacity were measured according to manufacturer’s protocol. ECAR values were normalized to total protein then plotted against time. Individual glycolysis and glycolytic capacity values were normalized to total protein level then to individual control from each biological repeat. a Stable HEK293 cells were overexpressed with either YFP (control), TDP-43 WT or TDP-43 ΔNLS . Data were analyzed by one-way ANOVA followed by post hoc Tukey’s test. b TDP-43 G298S patient iPSC and control iPSC were differentiated into motor neurons with our established protocol. On day 26 following differentiation, cells were treated with MG-132 (1 μM) for 24 h. Data were analyzed by Student’s t-test. c YFP (control), TDP-43 WT or TDP-43 ΔNLS stable cells were transiently transfected with various glycolysis shRNAs. Cell viability was measured via MTT assay. Decreased cell viability in all TDP-43 WT and TDP-43 ΔNLS with various glycolysis shRNA (n = 3). Data were analyzed by one-way ANOVA followed by post hoc Tukey’s test. All data are mean ± SE

    Journal: Acta Neuropathologica

    Article Title: TDP-43 impairs glycolysis by sequestering hexokinase 1 in amyotrophic lateral sclerosis

    doi: 10.1007/s00401-026-02996-6

    Figure Lengend Snippet: Pathological TDP-43 elicits glycolytic impairment in cells. a , b Agilent seahorse glycolytic stress test was performed on n = 3 independent biological repeats. Glycolysis and Glycolytic Capacity were measured according to manufacturer’s protocol. ECAR values were normalized to total protein then plotted against time. Individual glycolysis and glycolytic capacity values were normalized to total protein level then to individual control from each biological repeat. a Stable HEK293 cells were overexpressed with either YFP (control), TDP-43 WT or TDP-43 ΔNLS . Data were analyzed by one-way ANOVA followed by post hoc Tukey’s test. b TDP-43 G298S patient iPSC and control iPSC were differentiated into motor neurons with our established protocol. On day 26 following differentiation, cells were treated with MG-132 (1 μM) for 24 h. Data were analyzed by Student’s t-test. c YFP (control), TDP-43 WT or TDP-43 ΔNLS stable cells were transiently transfected with various glycolysis shRNAs. Cell viability was measured via MTT assay. Decreased cell viability in all TDP-43 WT and TDP-43 ΔNLS with various glycolysis shRNA (n = 3). Data were analyzed by one-way ANOVA followed by post hoc Tukey’s test. All data are mean ± SE

    Article Snippet: The following constructs pcDNA3.2-YFP (addgene #84910), pcDNA3.2-TDP-43 WT -YFP (human) (addgene #84911), pcDNA3.2-TDP-43 ΔNLS -YFP (human) (addgene #84912), pLD-puro-Cc-TARDBP-A315T_VA Plasmid (human TDP-43 A315T) (addgene #141329), pLD-puro-Cc-TARDBP-WT_VA Plasmid (human TDP-43 WT) (addgene #141327), and shRNA control (addgene: #8453) were purchased from addgene.

    Techniques: Control, Transfection, MTT Assay, shRNA