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plg1 backbone  (Addgene inc)


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    Structured Review

    Addgene inc plg1 backbone
    Plg1 Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plg1+backbone/pLG1-puro+Non-targeting+sgRNA+3+(Plasmid+%23109003)/pm41366211-475-18-20
    Average 93 stars, based on 6 article reviews
    plg1 backbone - by Bioz Stars, 2026-10
    93/100 stars

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    Cloning:

    Article Title: Chemically modified CRISPR-Cas9 enables targeting of individual G-quadruplex and i-motif structures, revealing ligand-dependent transcriptional perturbation.
    Article Snippet: Positive clones were then sequenced using the Genewiz service. .. AR TI CL E IN P RE SS ARTICLE IN PRESS Cloning of sgRNA in pLG1 plasmid The pLG1 backbone (Addgene #109003) was digested with BstXI and BlpI (FastDigest, ThermoFisher) for 1 hour at 37°C. .. Oligos containing the sgRNA sequence (Supplementary Table 4) were ordered from IDT and annealed in water at 10 μM in a thermocycler (37°C, 30 minutes- 95°C, 5 minutes and ramp down 5 degrees/minute to 25°C).

    Plasmid Preparation:

    Article Title: Chemically modified CRISPR-Cas9 enables targeting of individual G-quadruplex and i-motif structures, revealing ligand-dependent transcriptional perturbation.
    Article Snippet: Positive clones were then sequenced using the Genewiz service. .. AR TI CL E IN P RE SS ARTICLE IN PRESS Cloning of sgRNA in pLG1 plasmid The pLG1 backbone (Addgene #109003) was digested with BstXI and BlpI (FastDigest, ThermoFisher) for 1 hour at 37°C. .. Oligos containing the sgRNA sequence (Supplementary Table 4) were ordered from IDT and annealed in water at 10 μM in a thermocycler (37°C, 30 minutes- 95°C, 5 minutes and ramp down 5 degrees/minute to 25°C).



    Similar Products

    plg1 backbone  (Addgene inc)
    93
    Addgene inc plg1 backbone
    Plg1 Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plg1+backbone/pLG1-puro+Non-targeting+sgRNA+3+(Plasmid+%23109003)/pm41366211-475-18-20
    Average 93 stars, based on 1 article reviews
    plg1 backbone - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    plg1 lentiviral backbone  (Addgene inc)
    96
    Addgene inc plg1 lentiviral backbone
    ( A ) Human lung adenocarcinoma (A549) cells were genetically engineered with the lineage tracing components by <t>lentiviral</t> transduction with (1) Luciferase-Neomycin R and antibiotic-selected, (2) Cas9-mCherry and fluorescence-sorted, (3) serial, high-titer TargetSite-GFP and fluorescence-sorted, and finally (4) triple-sgRNA BFP-Puromycin R and fluorescence-sorted, thus producing lineage tracing-competent A549-LT cells. ( B ) Serial, high-titer TargetSite-GFP lentiviral transduction strategy to achieve high copy-number. ( C ) Cells with high-shifted GFP fluorescence after successive TargetSite-GFP infections, indicating increasing copy-number of the Target Site. ( D ) Sample of 5,000 A549-LT cells prior to injection to estimate initial clonal diversity. Shown is a heat-map of the fraction of shared intBCs in all cell–cell comparisons. On average, approximately 22,000 unique, high-quality intBCs were identified per random sample of 5,000 cells; thus, we estimate approximately 2,150 distinct clones per 5,000 cells (assuming 10.3 intBCs per clonal population; ) at the beginning of the experiment. ( E ) Comparison of the size of each clonal population observed in mouse M5k in a pre-implantation sample of 5,000 cells ( in vitro ) and post-sacrifice ( in vivo ). There is no correlation between the in vitro and in vivo population sizes (Spearman’s ρ =-0.026).
    Plg1 Lentiviral Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plg1+backbone/pCRISPRia-v2+(Plasmid+%2384832)/bio_rxiv__2020__04__16__045245-187-25-28
    Average 96 stars, based on 1 article reviews
    plg1 lentiviral backbone - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Human lung adenocarcinoma (A549) cells were genetically engineered with the lineage tracing components by lentiviral transduction with (1) Luciferase-Neomycin R and antibiotic-selected, (2) Cas9-mCherry and fluorescence-sorted, (3) serial, high-titer TargetSite-GFP and fluorescence-sorted, and finally (4) triple-sgRNA BFP-Puromycin R and fluorescence-sorted, thus producing lineage tracing-competent A549-LT cells. ( B ) Serial, high-titer TargetSite-GFP lentiviral transduction strategy to achieve high copy-number. ( C ) Cells with high-shifted GFP fluorescence after successive TargetSite-GFP infections, indicating increasing copy-number of the Target Site. ( D ) Sample of 5,000 A549-LT cells prior to injection to estimate initial clonal diversity. Shown is a heat-map of the fraction of shared intBCs in all cell–cell comparisons. On average, approximately 22,000 unique, high-quality intBCs were identified per random sample of 5,000 cells; thus, we estimate approximately 2,150 distinct clones per 5,000 cells (assuming 10.3 intBCs per clonal population; ) at the beginning of the experiment. ( E ) Comparison of the size of each clonal population observed in mouse M5k in a pre-implantation sample of 5,000 cells ( in vitro ) and post-sacrifice ( in vivo ). There is no correlation between the in vitro and in vivo population sizes (Spearman’s ρ =-0.026).

    Journal: bioRxiv

    Article Title: Single-cell lineages reveal the rates, routes, and drivers of metastasis in cancer xenografts

    doi: 10.1101/2020.04.16.045245

    Figure Lengend Snippet: ( A ) Human lung adenocarcinoma (A549) cells were genetically engineered with the lineage tracing components by lentiviral transduction with (1) Luciferase-Neomycin R and antibiotic-selected, (2) Cas9-mCherry and fluorescence-sorted, (3) serial, high-titer TargetSite-GFP and fluorescence-sorted, and finally (4) triple-sgRNA BFP-Puromycin R and fluorescence-sorted, thus producing lineage tracing-competent A549-LT cells. ( B ) Serial, high-titer TargetSite-GFP lentiviral transduction strategy to achieve high copy-number. ( C ) Cells with high-shifted GFP fluorescence after successive TargetSite-GFP infections, indicating increasing copy-number of the Target Site. ( D ) Sample of 5,000 A549-LT cells prior to injection to estimate initial clonal diversity. Shown is a heat-map of the fraction of shared intBCs in all cell–cell comparisons. On average, approximately 22,000 unique, high-quality intBCs were identified per random sample of 5,000 cells; thus, we estimate approximately 2,150 distinct clones per 5,000 cells (assuming 10.3 intBCs per clonal population; ) at the beginning of the experiment. ( E ) Comparison of the size of each clonal population observed in mouse M5k in a pre-implantation sample of 5,000 cells ( in vitro ) and post-sacrifice ( in vivo ). There is no correlation between the in vitro and in vivo population sizes (Spearman’s ρ =-0.026).

    Article Snippet: Guide RNAs for CRISPRi and CRISPRa experiments were chosen from the human CRISPRi/a v.2 libraries (Addgene #83969 and #83978, respectively) and were cloned into the pLG1 lentiviral backbone (Addgene #84832) using the annealing and ligation as previously described ( ).

    Techniques: Transduction, Luciferase, Fluorescence, Injection, Clone Assay, Comparison, In Vitro, In Vivo