plex307 lentiviral vector (Addgene inc)
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plex307 lentiviral vector
Plex307 Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plex307+lentiviral+vector/pLEX_307+(Plasmid+%2341392)/pmc12374242-429-20-28
Average 96 stars, based on 188 article reviews
Plex307 Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plex307+lentiviral+vector/pLEX_307+(Plasmid+%2341392)/pmc12374242-429-20-28
Average 96 stars, based on 188 article reviews
plex307 lentiviral vector - by Bioz Stars,
2026-11
96/100 stars
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other:Article Title: Treatment-induced tumor dormancy through YAP-mediated transcriptional reprogramming of the apoptotic pathway Article Snippet: All YAP1 cDNAs were subsequently shuttled into JP1722 expression vector using the BD Creator System (BD Biosciences). Article Title: Treatment-induced tumor dormancy through YAP-mediated transcriptional reprogramming of the apoptotic pathway Article Snippet: Animal models Xenograft studies: Female NCr nude mice, 7-weeks old (for PC-9 studies) and female NSG mice, 6-weeks old (HCC4006 and DFCI243) were purchased from Taconic Biosciences, Inc. and The Jackson Laboratory, respectively. Mutagenesis:Article Title: Treatment-induced tumor dormancy through YAP-mediated transcriptional reprogramming of the apoptotic pathway Article Snippet: The TEAD1 C359C mutation was generated into pRK5-myc- TEAD1 backbone (a gift from Kunliang Guan, Addgene plasmid #33109) by PCR using primers 5’-TCCCCAATGAGTGAATATATGATCAAC-3’ and 5’-GCGGTTTATTCGGTATACAAATCG-3’. .. Both wild-type myc-TEAD1 and the myc-TEAD1 C359S mutant cDNAs were amplified from the pRK5-backbone using primers 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGCCACCATGGAGCAAAAGCTCATCTCAG-3’ and 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTCAGTCCTTTACAAGCCTGTAAATATG-3’ and shuttled into the Amplification:Article Title: Treatment-induced tumor dormancy through YAP-mediated transcriptional reprogramming of the apoptotic pathway Article Snippet: The TEAD1 C359C mutation was generated into pRK5-myc- TEAD1 backbone (a gift from Kunliang Guan, Addgene plasmid #33109) by PCR using primers 5’-TCCCCAATGAGTGAATATATGATCAAC-3’ and 5’-GCGGTTTATTCGGTATACAAATCG-3’. .. Both wild-type myc-TEAD1 and the myc-TEAD1 C359S mutant cDNAs were amplified from the pRK5-backbone using primers 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGCCACCATGGAGCAAAAGCTCATCTCAG-3’ and 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTCAGTCCTTTACAAGCCTGTAAATATG-3’ and shuttled into the Article Title: Phosphorylation of TFCP2L1 by CDK1 is required for stem cell pluripotency and bladder carcinogenesis Article Snippet: .. The amplified Tfcp2l1 ORF constructs were cloned into pENTR4 plasmid (Invitrogen) modified by including CMV early enhancer/chicken β actin (CAG) promoter, pENTR4‐CAG (Heo et al , ), and then cloned into the Article Title: Eradicating Drug Tolerant Persister Cells in EGFR -Mutated Non-Small Cell Lung Cancer by Targeting TROP2 with CAR-T Cellular Therapy Article Snippet: .. Human TROP2 cDNA was PCR amplified from PC-9 cDNA using the primers 5’-GTGAGGCTAGCATCGTGCAGACCATCCCAGACG-3’ and 5’- TGTCGACTTAACGCGGACGATACCGAAATCCGGTAC-3’ and shuttled into the Plasmid Preparation:Article Title: Treatment-induced tumor dormancy through YAP-mediated transcriptional reprogramming of the apoptotic pathway Article Snippet: The TEAD1 C359C mutation was generated into pRK5-myc- TEAD1 backbone (a gift from Kunliang Guan, Addgene plasmid #33109) by PCR using primers 5’-TCCCCAATGAGTGAATATATGATCAAC-3’ and 5’-GCGGTTTATTCGGTATACAAATCG-3’. .. Both wild-type myc-TEAD1 and the myc-TEAD1 C359S mutant cDNAs were amplified from the pRK5-backbone using primers 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGCCACCATGGAGCAAAAGCTCATCTCAG-3’ and 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTCAGTCCTTTACAAGCCTGTAAATATG-3’ and shuttled into the Article Title: Phosphorylation of TFCP2L1 by CDK1 is required for stem cell pluripotency and bladder carcinogenesis Article Snippet: .. The amplified Tfcp2l1 ORF constructs were cloned into pENTR4 plasmid (Invitrogen) modified by including CMV early enhancer/chicken β actin (CAG) promoter, pENTR4‐CAG (Heo et al , ), and then cloned into the Article Title: Ascorbic Acid 2-Glucoside Stably Promotes the Primitiveness of Embryonic and Mesenchymal Stem Cells Through Ten-Eleven Translocation- and cAMP-Responsive Element-Binding Protein-1-Dependent Mechanisms. Article Snippet: Page 1 of 58 Antioxidants and Redox Signaling © Mary Ann Liebert, Inc. DOI: 10.1089/ars.2019.7743 1 A n ti o xi d an ts a n d R ed o x Si gn al in g A sc o rb ic a ci d 2 -g lu co si d e st ab ly p ro m o te s th e p ri m it iv e n es s o f e m b ry o n ic a n d m es en ch ym al s te m c el ls t h ro u gh T ET - an d C R EB 1 -d ep en d en t m ec h an is m s ( D O I: 1 0 .1 0 8 9 /a rs .2 0 1 9 .7 7 4 3 ) Th is p ap er h as b e e n p ee r- re vi e w ed a n d a cc ep te d f o r p u b lic at io n , b u t h as y et t o u n d er go c o p ye d it in g an d p ro o f co rr e ct io n . T h e fi n al p u b lis h ed v er si o n m ay d if fe r fr o m t h is p ro o f. Ascorbic acid 2-glucoside stably promotes the primitiveness of embryonic and mesenchymal stem cells through TET- and CREB1- dependent mechanisms Seungun Lee1,2,§, Jisun Lim1,2,§, Ji-Heon Lee3, Hyein Ju1,2, Jinbeom Heo1,2, YongHwan Kim1,2, Sujin Kim1,2, Hwan Yeul Yu1,2, Chae-Min Ryu1,2, So-Yeon Lee1, Jung-Min Han3, Yeon-Mok Oh4, Ho Lee5, Hyonchol Jang5, Tae-Joong Yoon6, Hee‐Sung Ahn7, Kyunggon Kim7, Hwa- Ryeon Kim8, Jae-Seok Roe8, Hyung-Min Chung3, Jaekyoung Son1,*, Jong Soo Kim3,*, Dong- Myung Shin1,2,* 1Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, Korea 2Department of Physiology, University of Ulsan College of Medicine, Seoul, 05505, Korea 3Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, 05029, Korea 4Department of Pulmonary and Critical Care Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, Korea 5Research Institute, Graduate School of Cancer Science and Policy, National Cancer Center, Goyang, Gyeonggi, 10408, Korea 6JJ.. MADIN Inc, Seoul, 08512, Korea 7 Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, Korea 8Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea §These authors equally contributed to this work.. Short title: Effect of AA2G on preserving primitive stem cells *Corresponding authors: Dong-Myung Shin, Ph.D. Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Pungnap-2 dong, Songpa-gu, Seoul, 05505, Korea Tel: 82-2-3010-2086; Fax: 82-2-3010-8493; Email: d0shin03@amc.seoul.kr D ow nl oa de d by U cs f L ib ra ry U ni ve rs ity o f C al if or ni a Sa n Fr an ci sc o fr om w w w .li eb er tp ub .c om a t 1 1/ 08 /1 9. Article Title: Eradicating Drug Tolerant Persister Cells in EGFR -Mutated Non-Small Cell Lung Cancer by Targeting TROP2 with CAR-T Cellular Therapy Article Snippet: .. Human TROP2 cDNA was PCR amplified from PC-9 cDNA using the primers 5’-GTGAGGCTAGCATCGTGCAGACCATCCCAGACG-3’ and 5’- TGTCGACTTAACGCGGACGATACCGAAATCCGGTAC-3’ and shuttled into the Clone Assay:Article Title: Treatment-induced tumor dormancy through YAP-mediated transcriptional reprogramming of the apoptotic pathway Article Snippet: The TEAD1 C359C mutation was generated into pRK5-myc- TEAD1 backbone (a gift from Kunliang Guan, Addgene plasmid #33109) by PCR using primers 5’-TCCCCAATGAGTGAATATATGATCAAC-3’ and 5’-GCGGTTTATTCGGTATACAAATCG-3’. .. Both wild-type myc-TEAD1 and the myc-TEAD1 C359S mutant cDNAs were amplified from the pRK5-backbone using primers 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGCCACCATGGAGCAAAAGCTCATCTCAG-3’ and 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTCAGTCCTTTACAAGCCTGTAAATATG-3’ and shuttled into the Article Title: Phosphorylation of TFCP2L1 by CDK1 is required for stem cell pluripotency and bladder carcinogenesis Article Snippet: .. The amplified Tfcp2l1 ORF constructs were cloned into pENTR4 plasmid (Invitrogen) modified by including CMV early enhancer/chicken β actin (CAG) promoter, pENTR4‐CAG (Heo et al , ), and then cloned into the Article Title: Ascorbic Acid 2-Glucoside Stably Promotes the Primitiveness of Embryonic and Mesenchymal Stem Cells Through Ten-Eleven Translocation- and cAMP-Responsive Element-Binding Protein-1-Dependent Mechanisms. Article Snippet: Page 1 of 58 Antioxidants and Redox Signaling © Mary Ann Liebert, Inc. DOI: 10.1089/ars.2019.7743 1 A n ti o xi d an ts a n d R ed o x Si gn al in g A sc o rb ic a ci d 2 -g lu co si d e st ab ly p ro m o te s th e p ri m it iv e n es s o f e m b ry o n ic a n d m es en ch ym al s te m c el ls t h ro u gh T ET - an d C R EB 1 -d ep en d en t m ec h an is m s ( D O I: 1 0 .1 0 8 9 /a rs .2 0 1 9 .7 7 4 3 ) Th is p ap er h as b e e n p ee r- re vi e w ed a n d a cc ep te d f o r p u b lic at io n , b u t h as y et t o u n d er go c o p ye d it in g an d p ro o f co rr e ct io n . T h e fi n al p u b lis h ed v er si o n m ay d if fe r fr o m t h is p ro o f. Ascorbic acid 2-glucoside stably promotes the primitiveness of embryonic and mesenchymal stem cells through TET- and CREB1- dependent mechanisms Seungun Lee1,2,§, Jisun Lim1,2,§, Ji-Heon Lee3, Hyein Ju1,2, Jinbeom Heo1,2, YongHwan Kim1,2, Sujin Kim1,2, Hwan Yeul Yu1,2, Chae-Min Ryu1,2, So-Yeon Lee1, Jung-Min Han3, Yeon-Mok Oh4, Ho Lee5, Hyonchol Jang5, Tae-Joong Yoon6, Hee‐Sung Ahn7, Kyunggon Kim7, Hwa- Ryeon Kim8, Jae-Seok Roe8, Hyung-Min Chung3, Jaekyoung Son1,*, Jong Soo Kim3,*, Dong- Myung Shin1,2,* 1Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, Korea 2Department of Physiology, University of Ulsan College of Medicine, Seoul, 05505, Korea 3Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, 05029, Korea 4Department of Pulmonary and Critical Care Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, Korea 5Research Institute, Graduate School of Cancer Science and Policy, National Cancer Center, Goyang, Gyeonggi, 10408, Korea 6JJ.. MADIN Inc, Seoul, 08512, Korea 7 Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, Korea 8Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea §These authors equally contributed to this work.. Short title: Effect of AA2G on preserving primitive stem cells *Corresponding authors: Dong-Myung Shin, Ph.D. Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Pungnap-2 dong, Songpa-gu, Seoul, 05505, Korea Tel: 82-2-3010-2086; Fax: 82-2-3010-8493; Email: d0shin03@amc.seoul.kr D ow nl oa de d by U cs f L ib ra ry U ni ve rs ity o f C al if or ni a Sa n Fr an ci sc o fr om w w w .li eb er tp ub .c om a t 1 1/ 08 /1 9. Construct:Article Title: Phosphorylation of TFCP2L1 by CDK1 is required for stem cell pluripotency and bladder carcinogenesis Article Snippet: .. The amplified Tfcp2l1 ORF constructs were cloned into pENTR4 plasmid (Invitrogen) modified by including CMV early enhancer/chicken β actin (CAG) promoter, pENTR4‐CAG (Heo et al , ), and then cloned into the Modification:Article Title: Phosphorylation of TFCP2L1 by CDK1 is required for stem cell pluripotency and bladder carcinogenesis Article Snippet: .. The amplified Tfcp2l1 ORF constructs were cloned into pENTR4 plasmid (Invitrogen) modified by including CMV early enhancer/chicken β actin (CAG) promoter, pENTR4‐CAG (Heo et al , ), and then cloned into the Over Expression:Article Title: Ascorbic Acid 2-Glucoside Stably Promotes the Primitiveness of Embryonic and Mesenchymal Stem Cells Through Ten-Eleven Translocation- and cAMP-Responsive Element-Binding Protein-1-Dependent Mechanisms. Article Snippet: Page 1 of 58 Antioxidants and Redox Signaling © Mary Ann Liebert, Inc. DOI: 10.1089/ars.2019.7743 1 A n ti o xi d an ts a n d R ed o x Si gn al in g A sc o rb ic a ci d 2 -g lu co si d e st ab ly p ro m o te s th e p ri m it iv e n es s o f e m b ry o n ic a n d m es en ch ym al s te m c el ls t h ro u gh T ET - an d C R EB 1 -d ep en d en t m ec h an is m s ( D O I: 1 0 .1 0 8 9 /a rs .2 0 1 9 .7 7 4 3 ) Th is p ap er h as b e e n p ee r- re vi e w ed a n d a cc ep te d f o r p u b lic at io n , b u t h as y et t o u n d er go c o p ye d it in g an d p ro o f co rr e ct io n . T h e fi n al p u b lis h ed v er si o n m ay d if fe r fr o m t h is p ro o f. Ascorbic acid 2-glucoside stably promotes the primitiveness of embryonic and mesenchymal stem cells through TET- and CREB1- dependent mechanisms Seungun Lee1,2,§, Jisun Lim1,2,§, Ji-Heon Lee3, Hyein Ju1,2, Jinbeom Heo1,2, YongHwan Kim1,2, Sujin Kim1,2, Hwan Yeul Yu1,2, Chae-Min Ryu1,2, So-Yeon Lee1, Jung-Min Han3, Yeon-Mok Oh4, Ho Lee5, Hyonchol Jang5, Tae-Joong Yoon6, Hee‐Sung Ahn7, Kyunggon Kim7, Hwa- Ryeon Kim8, Jae-Seok Roe8, Hyung-Min Chung3, Jaekyoung Son1,*, Jong Soo Kim3,*, Dong- Myung Shin1,2,* 1Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, Korea 2Department of Physiology, University of Ulsan College of Medicine, Seoul, 05505, Korea 3Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, 05029, Korea 4Department of Pulmonary and Critical Care Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, Korea 5Research Institute, Graduate School of Cancer Science and Policy, National Cancer Center, Goyang, Gyeonggi, 10408, Korea 6JJ.. MADIN Inc, Seoul, 08512, Korea 7 Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, Korea 8Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea §These authors equally contributed to this work.. Short title: Effect of AA2G on preserving primitive stem cells *Corresponding authors: Dong-Myung Shin, Ph.D. Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Pungnap-2 dong, Songpa-gu, Seoul, 05505, Korea Tel: 82-2-3010-2086; Fax: 82-2-3010-8493; Email: d0shin03@amc.seoul.kr D ow nl oa de d by U cs f L ib ra ry U ni ve rs ity o f C al if or ni a Sa n Fr an ci sc o fr om w w w .li eb er tp ub .c om a t 1 1/ 08 /1 9. Polymerase Chain Reaction:Article Title: Eradicating Drug Tolerant Persister Cells in EGFR -Mutated Non-Small Cell Lung Cancer by Targeting TROP2 with CAR-T Cellular Therapy Article Snippet: .. Human TROP2 cDNA was PCR amplified from PC-9 cDNA using the primers 5’-GTGAGGCTAGCATCGTGCAGACCATCCCAGACG-3’ and 5’- TGTCGACTTAACGCGGACGATACCGAAATCCGGTAC-3’ and shuttled into the Cloning:Article Title: Eradicating Drug Tolerant Persister Cells in EGFR -Mutated Non-Small Cell Lung Cancer by Targeting TROP2 with CAR-T Cellular Therapy Article Snippet: .. Human TROP2 cDNA was PCR amplified from PC-9 cDNA using the primers 5’-GTGAGGCTAGCATCGTGCAGACCATCCCAGACG-3’ and 5’- TGTCGACTTAACGCGGACGATACCGAAATCCGGTAC-3’ and shuttled into the |
