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plenticrisprv2 plasmid encoding guide rna targeting cypa  (Addgene inc)


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    Structured Review

    Addgene inc plenticrisprv2 plasmid encoding guide rna targeting cypa
    Plenticrisprv2 Plasmid Encoding Guide Rna Targeting Cypa, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 15695 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenticrisprv2/psPAX2+(Plasmid+%2312260)/bio_rxiv__64898__2026__10__07__757322-55-4-19
    Average 98 stars, based on 15695 article reviews
    plenticrisprv2 plasmid encoding guide rna targeting cypa - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Degraders of the dengue virus capsid protein exhibit differentiated pharmacology relative to capsid inhibitors
    Article Snippet: To generate plasmids for CRBN knockout, the guide RNA sequences were designed using Benchling, and oligos were synthesized from Integrated DNA Technology (IDT). .. The oligos 5’-caccgCAGGACGCTGCGCACAACAT-3’ and 5’-aaacATGTTGTGCGCAGCGTCCTGc-3’ were cloned into pLentiCRISPRV2 (Addgene, Plasmid #52961). ..

    Article Title: Degraders of the dengue virus capsid protein exhibit differentiated pharmacology relative to capsid inhibitors.
    Article Snippet: To generate plasmids for CRBN knockout, the guide RNA sequences were designed using Benchling, and oligos were synthesized from Integrated DNA Technology (IDT). .. The oligos 5’- caccgCAGGACGCTGCGCACAACAT -3’ and 5’- aaacATGTTGTGCGCAGCGTCCTGc -3’ were cloned into pLentiCRISPRV2 (Addgene, Plasmid #52961). ..

    Article Title: The PAXT connection is a modifier of rare codon-enriched protein expression
    Article Snippet: .. Oligonucleotides encoding these sgRNAs were synthesizedby IntegratedDNATechnologies (IDT) and separately cloned into pLentiCRISPRv2 (a gift from Feng Zhang; Addgene vector # 52961)71 vector. ..

    Article Title: Gα12/13 deficiency attenuates obesity-associated adipose tissue inflammation and promotes energy expenditure
    Article Snippet: .. Each sgRNAsequences forGna12 (TGTTGTCGAAGATGGTGTCG) and Gna13 (CTTGGATAAACTTGGAGTAC) were cloned into pLentiCRISPRv2 (Addgene, #52961) and pLentiCRISPRv2-Blast (Addgene, #83480) using BsmBIv2 (NEB). .. For Gα12 and Gα13 overexpression, a codon-optimized murine Gα12 and Gα13, their constitutively active mutants (Gα12_ Q229L and Gα13_ Q226L)19,23,40, as well as human Gα12 and Gα13 constructs, were synthesized and subcloned into pcDNA3.1 (Thermo-Fisher), pCW57.1 (Addgene #41393) or pCW57-MCS1-P2A-MCS2 (Blast) (Addgene #80921).

    Plasmid Preparation:

    Article Title: Degraders of the dengue virus capsid protein exhibit differentiated pharmacology relative to capsid inhibitors
    Article Snippet: To generate plasmids for CRBN knockout, the guide RNA sequences were designed using Benchling, and oligos were synthesized from Integrated DNA Technology (IDT). .. The oligos 5’-caccgCAGGACGCTGCGCACAACAT-3’ and 5’-aaacATGTTGTGCGCAGCGTCCTGc-3’ were cloned into pLentiCRISPRV2 (Addgene, Plasmid #52961). ..

    Article Title: In vivo CRISPR/Cas9 screens identify new regulators of B cell activation and plasma cell differentiation
    Article Snippet: .. LV sgRNA expression vectors were generated using pLentiCRISPRv1 (Addgene plasmid no. 49535) or pLentiCRISPRv2 (Addgene plasmid no. 52961). ..

    Article Title: Degraders of the dengue virus capsid protein exhibit differentiated pharmacology relative to capsid inhibitors.
    Article Snippet: To generate plasmids for CRBN knockout, the guide RNA sequences were designed using Benchling, and oligos were synthesized from Integrated DNA Technology (IDT). .. The oligos 5’- caccgCAGGACGCTGCGCACAACAT -3’ and 5’- aaacATGTTGTGCGCAGCGTCCTGc -3’ were cloned into pLentiCRISPRV2 (Addgene, Plasmid #52961). ..

    Article Title: The PAXT connection is a modifier of rare codon-enriched protein expression
    Article Snippet: .. Oligonucleotides encoding these sgRNAs were synthesizedby IntegratedDNATechnologies (IDT) and separately cloned into pLentiCRISPRv2 (a gift from Feng Zhang; Addgene vector # 52961)71 vector. ..

    Expressing:

    Article Title: In vivo CRISPR/Cas9 screens identify new regulators of B cell activation and plasma cell differentiation
    Article Snippet: .. LV sgRNA expression vectors were generated using pLentiCRISPRv1 (Addgene plasmid no. 49535) or pLentiCRISPRv2 (Addgene plasmid no. 52961). ..

    Generated:

    Article Title: In vivo CRISPR/Cas9 screens identify new regulators of B cell activation and plasma cell differentiation
    Article Snippet: .. LV sgRNA expression vectors were generated using pLentiCRISPRv1 (Addgene plasmid no. 49535) or pLentiCRISPRv2 (Addgene plasmid no. 52961). ..

    Article Title: TMEM106B mediates ACE2-independent replication of the SARS-CoV-2 S-E484D variant in airway-derived cell models
    Article Snippet: .. pLentiCRISPRv2 (Addgene ID #98290) derived vector targeting ACE2 has been described before ( ). pLentiCRISPRv2 targeting TMEM106B was similarly generated. .. pLentiCRISPRv2 (Addgene ID #98290) derived vector targeting ACE2 has been described before ( ).

    other:

    Article Title: In vivo CRISPR/Cas9 screens identify new regulators of B cell activation and plasma cell differentiation.
    Article Snippet: Finally, the sections were incubated with donkey anti-rabbit IgG (H+L) Alexa Fluor 568 (A10042; Invitrogen) diluted in PBS containing 2 μg/ml antimouse CD16/32 and 1% BSA and mounted with ProLong Diamond Antifade Mountant (Invitrogen).

    Derivative Assay:

    Article Title: TMEM106B mediates ACE2-independent replication of the SARS-CoV-2 S-E484D variant in airway-derived cell models
    Article Snippet: .. pLentiCRISPRv2 (Addgene ID #98290) derived vector targeting ACE2 has been described before ( ). pLentiCRISPRv2 targeting TMEM106B was similarly generated. .. pLentiCRISPRv2 (Addgene ID #98290) derived vector targeting ACE2 has been described before ( ).



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