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plc/prf/5  (ATCC)


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    Structured Review

    ATCC plc/prf/5
    Plc/Prf/5, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1245 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plc+prf+5/PLC%2FPRF%2F5/custom%40crl-8024%4042615099
    Average 96 stars, based on 1245 article reviews
    plc/prf/5 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Planar Chromatography:

    Article Title: ISX-TWIST1 reprograms CD47-inflammasome signaling to reshape the immune microenvironment in liver cancer.
    Article Snippet: .. Human hepatocellular carcinoma (HCC) cell lines, including HepG2 (RRID:CVCL_0027), Hep3B (RRID:CVCL_0326), SNU-423 (RRID:CVCL_0366), Huh7 (RRID:CVCL_0336), PLC/PRF/5 (RRID:CVCL_0485), HA22T (RRID:CVCL_7046), and HCC36 (RRID:CVCL_VI190), were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) or the Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan) between January and March 2023. ..

    Article Title: CCNA2, MAD2L1, AURKA, and PTTG1 promote proliferation and migration in hepatocellular carcinoma.
    Article Snippet: .. Cell lines and culture conditions LIHC cell lines, including HepG2 (HB-8065), Hep3B (HB-8064), and PLC/PRF/5 (CRL-8024) were obtained from the American Type Culture Collection (ATCC, USA), while Huh7, JHH-1, JHH-7, Li-7, and HLF were obtained from the JCRB Cell Bank (Japan). .. The SNU series cell lines (SNU182, SNU-387, SNU-398, SNU-423, SNU-449, and SNU-475) were obtained from the Korean Cell Line Bank (KCLB).

    Article Title: Impeding hypoxia-driven tumor progression in hepatocellular carcinoma: clinical and pre-clinical analyses using 2D and 3D in vitro models.
    Article Snippet: .. 165 Cell culture and reagents 166 AR TIC LE IN PR ES S Human HCC cell lines HepG2, Hep3B, PLC/PRF/5, SNU-387, SNU-423, SNU-167 449 and SNU-475 were obtained from the American Type Culture Collection 168 (Manassas, VA, USA), while Huh-7 cell line was obtained from the Japanese 169 Collection of Research Bioresources Cell Bank (Tokyo, Japan). .. HIF1A knockout 170 (KO) cells were generated by Cyagen Biosciences (Santa Clara, CA, USA) 171 using CRISPR/Cas9 technology.

    Article Title: Loss of SALL1 Promotes Hepatocellular Carcinoma Growth and Is Associated with Poor Clinical Outcome.
    Article Snippet: .. HepG2, Hep3B, PLC/PRF/5 and SK-Hep-1 cells were obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA). .. 293FT cells were purchased from Thermo-Fisher Scientific (Waltham, MA, USA), and Huh7 cells were provided by the Laboratory of Human Carcinogenesis, National Cancer Institute (Bethesda, MD, USA).

    Article Title: Lactate-driven pyrimidine synthesis promotes ferroptosis resistance in hepatocellular carcinoma
    Article Snippet: The human hepatic stellate cell line LX-2 (Sigma, St. Louis, MO, USA) was cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (P/S). .. The human liver cancer cell lines Huh7 (Korean Cell Line Bank, Seoul, Korea) and PLC/PRF/5 (ATCC, Manassas, VA, USA) were cultured in DMEM and Roswell Park Memorial Institute (RPMI) 1640 media containing 10% FBS and 1% P/S. .. The human monocytic cell line THP-1 (Korean Cell Line Bank) was cultured in RPMI 1640 medium containing 10% FBS and 1% P/S.

    Article Title: H4K5 lactylation - ENO2 loop drives glycolysis and HCC progression
    Article Snippet: .. Three human HCC cell lines, HLF (RRID: CVCL_2947), PLC/PRF/5 (RRID: CVCL_0485), and SNU475 (RRID: CVCL_0497), were purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan) and the American Type Culture Collection (Manassas, VA, USA). ..

    Article Title: Lactate-driven pyrimidine synthesis promotes ferroptosis resistance in hepatocellular carcinoma.
    Article Snippet: Cell line culture and preparation of Conditioned medium (CM) from liver cancer cells The human hepatic stellate cell line LX-2 (Sigma, St. Louis, MO, USA) was cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (P/S). .. The human liver cancer cell lines Huh7 (Korean Cell Line Bank, Seoul, Korea) and PLC/PRF/5 (ATCC, Manassas, VA, USA) were cultured in DMEM and Roswell Park Memorial Institute (RPMI) 1640 media containing 10% FBS and 1% P/S. .. The human monocytic cell line THP-1 (Korean Cell Line Bank) was cultured in RPMI 1640 medium containing 10% FBS and 1% P/S.

    Article Title: Enteroviral epitope mimicry enables NK cell-mediated targeting of ASPH in hepatocellular carcinoma
    Article Snippet: Following incubation with HRP-conjugated secondary antibody (Sigma) and TMB substrate (Thermo Fisher), antibody binding signal was measured at 450 nm using a spectrophotometer (VANTAstar, BMG LABTECH). .. Hep3B, HepG2, PLC/PRF/5, SNU182, SNU387, SNU398, SNU449 and SNU475 were obtained from ATCC, Huh1, Huh4, Huh7, and HLE were obtained from HSRRB/JCRB, SNU761 was obtained from KCLB, MHCC-97 and SMMC-7721 were obtained from Dr. Qin (Fudan University, Shanghai, China), and Hep-40 and KMCH-1 were obtained from Dr. Conner (NCI, Bethesda, USA) and Dr. Ho (NCI, Bethesda, USA), respectively. .. Huh7, Huh4, PLC/PRF/5, KMCH-1, and MHCC-97 cells were maintained in Dulbecco’s modified Eagle Medium (Thermo Fisher) supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine (Gibco), 1X penicillin and streptomycin (Gibco).

    Cell Culture:

    Article Title: Impeding hypoxia-driven tumor progression in hepatocellular carcinoma: clinical and pre-clinical analyses using 2D and 3D in vitro models.
    Article Snippet: .. 165 Cell culture and reagents 166 AR TIC LE IN PR ES S Human HCC cell lines HepG2, Hep3B, PLC/PRF/5, SNU-387, SNU-423, SNU-167 449 and SNU-475 were obtained from the American Type Culture Collection 168 (Manassas, VA, USA), while Huh-7 cell line was obtained from the Japanese 169 Collection of Research Bioresources Cell Bank (Tokyo, Japan). .. HIF1A knockout 170 (KO) cells were generated by Cyagen Biosciences (Santa Clara, CA, USA) 171 using CRISPR/Cas9 technology.

    Article Title: Lactate-driven pyrimidine synthesis promotes ferroptosis resistance in hepatocellular carcinoma
    Article Snippet: The human hepatic stellate cell line LX-2 (Sigma, St. Louis, MO, USA) was cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (P/S). .. The human liver cancer cell lines Huh7 (Korean Cell Line Bank, Seoul, Korea) and PLC/PRF/5 (ATCC, Manassas, VA, USA) were cultured in DMEM and Roswell Park Memorial Institute (RPMI) 1640 media containing 10% FBS and 1% P/S. .. The human monocytic cell line THP-1 (Korean Cell Line Bank) was cultured in RPMI 1640 medium containing 10% FBS and 1% P/S.

    Article Title: Lactate-driven pyrimidine synthesis promotes ferroptosis resistance in hepatocellular carcinoma.
    Article Snippet: Cell line culture and preparation of Conditioned medium (CM) from liver cancer cells The human hepatic stellate cell line LX-2 (Sigma, St. Louis, MO, USA) was cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (P/S). .. The human liver cancer cell lines Huh7 (Korean Cell Line Bank, Seoul, Korea) and PLC/PRF/5 (ATCC, Manassas, VA, USA) were cultured in DMEM and Roswell Park Memorial Institute (RPMI) 1640 media containing 10% FBS and 1% P/S. .. The human monocytic cell line THP-1 (Korean Cell Line Bank) was cultured in RPMI 1640 medium containing 10% FBS and 1% P/S.



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