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plasmid psp72  (Promega)

 
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    Structured Review

    Promega plasmid psp72
    Plasmid Psp72, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+psp72/psp72/pmc10945814__pnas__2321722121__sapp-107-0-9
    Average 90 stars, based on 1 article reviews
    plasmid psp72 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Supporting Information for An Unusual Aromatase/Cyclase Programs the Formation of the Phenyldimethylanthrone Framework in Anthrabenzoxocinones and Fasamycin
    Article Snippet: Lab storage pSP72 Plasmid providing the bla template, Ampicillin Promega pACYCDuet-1 Plasmid providing the cmr template, chloramphenicol Lab storage pET21a Plasmid for protein overexpression in E. coli, Ampicillin Novagen pET28a Plasmid for protein overexpression in E. coli, kanamycin Novagen pWY45 pXY200 derivative containing a cassette of oriT and acc(3) IV from pIB139, apramycin and Thiostrepton Lab storage pIJ778 Plasmid providing the aada template (4) pIB139 Integrative plasmid for gene complementation, apramycin (5) pJF Plasmid for protein overexpression in E. coli, containing tac promoter, Ampicillin (6) 4E10 Cosmid containing entire (−)-abx cluster Lab storage pJTU84 pET21a derived plasmid for Abx(+)S expression This study pJTU85 pJF derived plasmid for Abx(+)C expression This study pJTU86 pJF derived plasmid for Abx(+)D expression This study pJTU87 pJF derived plasmid for Abx(+)R expression This study pJTU88 pJF derived plasmid for Abx(+)M expression This study pJTU89 pET28a derived plasmid for FabD expression This study pJTU90 pET28a derived plasmid for MatB expression This study pJTU91 pET28a derived plasmid for SAM synthetase expression This study pJTU92 pET28a derived plasmid for Abx(-)S expression This study pJTU93 pET28a derived plasmid for Abx(-)C expression This study pJTU94 pET28a derived plasmid for Abx(-)D expression This study pJTU95 pET28a derived plasmid for Abx(-)R expression This study pJTU96 pET28a derived plasmid for Abx(-)M expression This study pJTU97 pJF derived plasmid for FasC expression This study pJTU98 pJF derived plasmid for FasD expression This study pJTU99 pJF derived plasmid for FasL expression This study pJTU100 pJF derived plasmid for FasR expression This study pJTU101 pJF derived plasmid for FasT expression This study pJTU102 pWY45 derived plasmid for FasA and FasB expression This study pJTU103 pJTU2554 derative, containing abx(+)P, K, S, C, D, M, R as well as a few of other flanking genes This study pJTU104 pJTU103 derivative, abx(+)C was deleted This study pJTU105 pJTU103 derivative, abx(+)D was deleted This study pJTU106 pJTU103 derivative, abx(+)M was deleted This study pJTU107 4E10 derivative, abx(-)DH was deleted This study Table S5.

    Article Title: The Developmental Shift of NMDA Receptor Composition Proceeds Independently of GluN2 Subunit-Specific GluN2 C-Terminal Sequences
    Article Snippet: An artificial polylinker containing Xho1 and HindIII restriction sites was inserted into the NotI and SalI sites of pSP72 (Promega), resulting in pSP72-L. A Diphtheria Toxin A (DT-A) fragment driven by an MC1 promoter with a mouse SV40 polyadenylation signal was digested with HindIII and XhoI, and inserted into the HindIII and SalI sites of pSP72-L, creating pSP72-L-DT-ApA ( ).

    Clone Assay:

    Article Title: Capsular polysaccharide production and serum survival of Vibrio vulnificus are dependent on antitermination control by RfaH.
    Article Snippet: .. Following digestion with XbaI and PstI, the resulting fragment was cloned into these same sites on plasmid pSP72 (Promega, Madison, WI, USA) to create pVV37. .. The insert region of pVV37 was confirmed by sequencing with relevant primers and BigDye v3.1 (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s protocol.

    Plasmid Preparation:

    Article Title: Capsular polysaccharide production and serum survival of Vibrio vulnificus are dependent on antitermination control by RfaH.
    Article Snippet: .. Following digestion with XbaI and PstI, the resulting fragment was cloned into these same sites on plasmid pSP72 (Promega, Madison, WI, USA) to create pVV37. .. The insert region of pVV37 was confirmed by sequencing with relevant primers and BigDye v3.1 (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s protocol.

    Article Title: Human Cytomegalovirus Tropism Modulator UL148 Interacts with SEL1L, a Cellular Factor That Governs Endoplasmic Reticulum-Associated Degradation of the Viral Envelope Glycoprotein gO
    Article Snippet: .. An intermediate BAC, TB_148bla, was first constructed by replacing the UL148 open reading frame (ORF) of TB_148 HA with a beta-lactamase ( bla ) cassette PCR amplified from plasmid pSP72 (Promega, Inc., Madison, WI) using primers TB_148bla_FWD and TB_148bla_REV. ..

    Article Title: Comparison of actinomycin peptide synthetase formation in Streptomyces chrysomallus and Streptomyces antibioticus.
    Article Snippet: Funding information Deutsche Forschungsgemeinschaft, Grant number: Ke 452/8-4; Cluster of Excellence “Unifying Concepts in Catalysis” at the Technische Universität Berlin; German Research Foundation (DFG Rückkehrstipendium) Actinomycin peptide synthetase genes constitute two oppositely oriented transcriptional units, acmADR, andacmBC, separated by a non-coding intergenic region.Gene constructs of the intergenic region together with its adjoining gene acmA or acmB from the actinomycin biosynthetic gene cluster of Streptomyces chrysomallus were transferred into Streptomyces lividansTK64.. Each construct expressed the respective synthetase indicating divergent promoters.. Primer extension revealed for both directions−10 and−35 boxes similar toσ-dependent promoters from Streptomyces and E. coli.

    Article Title: Mechanisms underlying Myosin 10′s contribution to the maintenance of mitotic spindle bipolarity
    Article Snippet: Myosin 10 (Myo10) couples microtubules and integrin-based adhesions to movement along actin filaments via its microtubule-binding MyTH4 domain and integrin-binding FERM domain, respectively.. Here we show that Myo10 depleted HeLa cells and mouse embryo fibroblasts (MEFs) both exhibit a pronounced increase in the frequency of multipolar spindles.. Staining of unsynchronized metaphase cells showed that the primary driver of spindle multipolarity in Myo10 depleted MEFs and in Myo10 depleted HeLa cells lacking supernumerary centrosomes is pericentriolar material (PCM) fragmentation, which creates y-tubulin-positive acentriolar foci that serve as extra spindle poles.

    Article Title: Mechanisms underlying Myosin 10′s contribution to the maintenance of mitotic spindle bipolarity
    Article Snippet: .. For cloning convenience, ∼20 bp taken from the backbone of plasmid pSP72 (Promega, P2191) was added onto the outside end of both 5′ and 3′HDRs. ..

    BAC Assay:

    Article Title: Human Cytomegalovirus Tropism Modulator UL148 Interacts with SEL1L, a Cellular Factor That Governs Endoplasmic Reticulum-Associated Degradation of the Viral Envelope Glycoprotein gO
    Article Snippet: .. An intermediate BAC, TB_148bla, was first constructed by replacing the UL148 open reading frame (ORF) of TB_148 HA with a beta-lactamase ( bla ) cassette PCR amplified from plasmid pSP72 (Promega, Inc., Madison, WI) using primers TB_148bla_FWD and TB_148bla_REV. ..

    Construct:

    Article Title: Human Cytomegalovirus Tropism Modulator UL148 Interacts with SEL1L, a Cellular Factor That Governs Endoplasmic Reticulum-Associated Degradation of the Viral Envelope Glycoprotein gO
    Article Snippet: .. An intermediate BAC, TB_148bla, was first constructed by replacing the UL148 open reading frame (ORF) of TB_148 HA with a beta-lactamase ( bla ) cassette PCR amplified from plasmid pSP72 (Promega, Inc., Madison, WI) using primers TB_148bla_FWD and TB_148bla_REV. ..

    Polymerase Chain Reaction:

    Article Title: Human Cytomegalovirus Tropism Modulator UL148 Interacts with SEL1L, a Cellular Factor That Governs Endoplasmic Reticulum-Associated Degradation of the Viral Envelope Glycoprotein gO
    Article Snippet: .. An intermediate BAC, TB_148bla, was first constructed by replacing the UL148 open reading frame (ORF) of TB_148 HA with a beta-lactamase ( bla ) cassette PCR amplified from plasmid pSP72 (Promega, Inc., Madison, WI) using primers TB_148bla_FWD and TB_148bla_REV. ..

    Amplification:

    Article Title: Human Cytomegalovirus Tropism Modulator UL148 Interacts with SEL1L, a Cellular Factor That Governs Endoplasmic Reticulum-Associated Degradation of the Viral Envelope Glycoprotein gO
    Article Snippet: .. An intermediate BAC, TB_148bla, was first constructed by replacing the UL148 open reading frame (ORF) of TB_148 HA with a beta-lactamase ( bla ) cassette PCR amplified from plasmid pSP72 (Promega, Inc., Madison, WI) using primers TB_148bla_FWD and TB_148bla_REV. ..

    Cloning:

    Article Title: Mechanisms underlying Myosin 10′s contribution to the maintenance of mitotic spindle bipolarity
    Article Snippet: Myosin 10 (Myo10) couples microtubules and integrin-based adhesions to movement along actin filaments via its microtubule-binding MyTH4 domain and integrin-binding FERM domain, respectively.. Here we show that Myo10 depleted HeLa cells and mouse embryo fibroblasts (MEFs) both exhibit a pronounced increase in the frequency of multipolar spindles.. Staining of unsynchronized metaphase cells showed that the primary driver of spindle multipolarity in Myo10 depleted MEFs and in Myo10 depleted HeLa cells lacking supernumerary centrosomes is pericentriolar material (PCM) fragmentation, which creates y-tubulin-positive acentriolar foci that serve as extra spindle poles.

    Article Title: Mechanisms underlying Myosin 10′s contribution to the maintenance of mitotic spindle bipolarity
    Article Snippet: .. For cloning convenience, ∼20 bp taken from the backbone of plasmid pSP72 (Promega, P2191) was added onto the outside end of both 5′ and 3′HDRs. ..



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    Image Search Results


    A) Quantification of BORC KD TDP-43 immunofluorescence microscopy. Both BORC genes tested (S2 and S6) show significant decreases in untagged TDP-43 levels compared to a NT guide, indicated by blue dots. N=4 wells per genotype, 9 images per well (small gray dots). Significant p-values indicated on graph. B) Representative images of TDP-43 immunofluorescence imaging with BORCS6 KD. sgRNA plasmids contain a cytoplasmic BFP, enabling identification of cells expressing the sgRNA. Scale bar represents 20 µm. C) Schematic of pLentiCRISPR plasmid. pLentiCRISPR enables lentiviral delivery of a plasmid expressing an sgRNA of interest under a U6 promoter and Cas9 under an Ef1alpha promoter. This enables knockout of a gene of interest targeted by an sgRNA. D) Representative images of BORCS7 KO TDP-43 IF with lysosomes stained with anti-H4A3 antibody. Scale bar is 20 µm. E) Quantification of TDP-43 immunofluorescence on BORCS7 knockout neurons. Both BORCS7 guides significantly reduced the amount of TDP-43 IF signal compared to a NT guide, light blue dots. N=10 wells, 9 images per well (light gray dots). Significant p-values indicated on graph.

    Journal: bioRxiv

    Article Title: Maintenance of neuronal TDP-43 expression requires axonal lysosome transport

    doi: 10.1101/2024.09.30.615241

    Figure Lengend Snippet: A) Quantification of BORC KD TDP-43 immunofluorescence microscopy. Both BORC genes tested (S2 and S6) show significant decreases in untagged TDP-43 levels compared to a NT guide, indicated by blue dots. N=4 wells per genotype, 9 images per well (small gray dots). Significant p-values indicated on graph. B) Representative images of TDP-43 immunofluorescence imaging with BORCS6 KD. sgRNA plasmids contain a cytoplasmic BFP, enabling identification of cells expressing the sgRNA. Scale bar represents 20 µm. C) Schematic of pLentiCRISPR plasmid. pLentiCRISPR enables lentiviral delivery of a plasmid expressing an sgRNA of interest under a U6 promoter and Cas9 under an Ef1alpha promoter. This enables knockout of a gene of interest targeted by an sgRNA. D) Representative images of BORCS7 KO TDP-43 IF with lysosomes stained with anti-H4A3 antibody. Scale bar is 20 µm. E) Quantification of TDP-43 immunofluorescence on BORCS7 knockout neurons. Both BORCS7 guides significantly reduced the amount of TDP-43 IF signal compared to a NT guide, light blue dots. N=10 wells, 9 images per well (light gray dots). Significant p-values indicated on graph.

    Article Snippet: TDP-43 coding sequence and 3’UTR were cloned into a plasmid expressing 24 SunTag repeats under an Ef1alpha promoter followed by 24 PP7 stem loops (modified from Addgene 74928).

    Techniques: Immunofluorescence, Microscopy, Imaging, Expressing, Plasmid Preparation, Knock-Out, Staining