prs314 (ATCC)

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ATCC prs314
Prs314, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 20065 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+prs314/Plasmid/pmc12738073-141-16-17
Average 99 stars, based on 20065 article reviews

prs314 - by Bioz Stars, 2026-10

99/100 stars

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Expressing:

Article Title: PDI Improves Secretion of Redox-Inactive ?-glucosidase
Article Snippet: .. Plasmids The single copy expression plasmid pRS314-β was constructed from a secretion-modified full-length β-glucosidase with an N-terminal synthetic pre-pro secretion signal sequence and a 10-amino-acid C-terminal c- myc detection tag under the control of a GAL1-10 promoter and the plasmid pRS314 (ATCC) containing the TRP1 marker as reported previously 5 . .. Plasmids The single copy expression plasmid pRS314-β was constructed from a secretion-modified full-length β-glucosidase with an N-terminal synthetic pre-pro secretion signal sequence and a 10-amino-acid C-terminal c- myc detection tag under the control of a GAL1-10 promoter and the plasmid pRS314 (ATCC) containing the TRP1 marker as reported previously 5 .

Article Title: PDI Improves Secretion of Redox-Inactive ?-glucosidase
Article Snippet: Images were quantitated using ImageQuant software Version 5.2 (Molecular Dynamics). .. The single copy expression plasmid pRS314-β was constructed from a secretion-modified full-length β-glucosidase with an N-terminal synthetic pre-pro secretion signal sequence and a 10-amino-acid C-terminal c- myc detection tag under the control of a GAL1-10 promoter and the plasmid pRS314 (ATCC) containing the TRP1 marker as reported previously 5 . .. Site-directed mutagenesis was performed using the QuikChange Site-Directed Mutagenesis kit (Stratagene) according to manufacturer instructions using plasmid pRS314-β to create pRS314-βC75S containing the C75S mutation.

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Article Title: PDI Improves Secretion of Redox-Inactive ?-glucosidase
Article Snippet: Plasmids The single copy expression plasmid pRS314-β was constructed from a secretion-modified full-length β-glucosidase with an N-terminal synthetic pre-pro secretion signal sequence and a 10-amino-acid C-terminal c- myc detection tag under the control of a GAL1-10 promoter and the plasmid pRS314 (ATCC) containing the TRP1 marker as reported previously 5 . .. Plasmids The single copy expression plasmid pRS314-β was constructed from a secretion-modified full-length β-glucosidase with an N-terminal synthetic pre-pro secretion signal sequence and a 10-amino-acid C-terminal c- myc detection tag under the control of a GAL1-10 promoter and the plasmid pRS314 (ATCC) containing the TRP1 marker as reported previously 5 . .. Site-directed mutagenesis was performed using the QuikChange Site-Directed Mutagenesis kit (Stratagene) according to manufacturer instructions using plasmid pRS314-β to create pRS314-βC75S containing the C75S mutation.

other:

Article Title: PDI Improves Secretion of Redox-Inactive ?-glucosidase
Article Snippet: Images were quantitated using ImageQuant software Version 5.2 (Molecular Dynamics).

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