Review



mruby2 n1  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc mruby2 n1
    Mruby2 N1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+mruby2+n1/mRuby2-N1+(Plasmid+%2354614)/us12351877-394-25-31
    Average 93 stars, based on 18 article reviews
    mruby2 n1 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Transfection:

    Article Title: Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma.
    Article Snippet: In vitro and in vivo grade siRNA against SH3GL2 (si-SH3GL2) and nonspecific control siRNA (si-NC) were obtained from RiboBio (Guangzhou, China) and performed according to the manufacturer’s protocol. .. The Y79 cells were transfected with plasmid mRuby2-N1 (empty vector, OE-NC, cat. no. 54614; Addgene, Watertown, MA, USA) or plasmid EndophilinA1-mRuby2 (overexpression SH3GL2, OESH3GL2, cat. no. 171945; Addgene) by Lipofectamine 3000 transfection reagent (Invitrogen) according to the manufacturer’s protocol. ..

    Article Title: Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma
    Article Snippet: In vitro and in vivo grade siRNA against SH3GL2 (si-SH3GL2) and nonspecific control siRNA (si-NC) were obtained from RiboBio (Guangzhou, China) and performed according to the manufacturer's protocol. .. The Y79 cells were transfected with plasmid mRuby2-N1 (empty vector, OE-NC, cat. no. 54614; Addgene, Watertown, MA, USA) or plasmid EndophilinA1-mRuby2 (overexpression SH3GL2, OE-SH3GL2, cat. no. 171945; Addgene) by Lipofectamine 3000 transfection reagent (Invitrogen) according to the manufacturer's protocol. ..

    Plasmid Preparation:

    Article Title: Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma.
    Article Snippet: In vitro and in vivo grade siRNA against SH3GL2 (si-SH3GL2) and nonspecific control siRNA (si-NC) were obtained from RiboBio (Guangzhou, China) and performed according to the manufacturer’s protocol. .. The Y79 cells were transfected with plasmid mRuby2-N1 (empty vector, OE-NC, cat. no. 54614; Addgene, Watertown, MA, USA) or plasmid EndophilinA1-mRuby2 (overexpression SH3GL2, OESH3GL2, cat. no. 171945; Addgene) by Lipofectamine 3000 transfection reagent (Invitrogen) according to the manufacturer’s protocol. ..

    Article Title: Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma
    Article Snippet: In vitro and in vivo grade siRNA against SH3GL2 (si-SH3GL2) and nonspecific control siRNA (si-NC) were obtained from RiboBio (Guangzhou, China) and performed according to the manufacturer's protocol. .. The Y79 cells were transfected with plasmid mRuby2-N1 (empty vector, OE-NC, cat. no. 54614; Addgene, Watertown, MA, USA) or plasmid EndophilinA1-mRuby2 (overexpression SH3GL2, OE-SH3GL2, cat. no. 171945; Addgene) by Lipofectamine 3000 transfection reagent (Invitrogen) according to the manufacturer's protocol. ..

    Over Expression:

    Article Title: Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma.
    Article Snippet: In vitro and in vivo grade siRNA against SH3GL2 (si-SH3GL2) and nonspecific control siRNA (si-NC) were obtained from RiboBio (Guangzhou, China) and performed according to the manufacturer’s protocol. .. The Y79 cells were transfected with plasmid mRuby2-N1 (empty vector, OE-NC, cat. no. 54614; Addgene, Watertown, MA, USA) or plasmid EndophilinA1-mRuby2 (overexpression SH3GL2, OESH3GL2, cat. no. 171945; Addgene) by Lipofectamine 3000 transfection reagent (Invitrogen) according to the manufacturer’s protocol. ..

    Article Title: Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma
    Article Snippet: In vitro and in vivo grade siRNA against SH3GL2 (si-SH3GL2) and nonspecific control siRNA (si-NC) were obtained from RiboBio (Guangzhou, China) and performed according to the manufacturer's protocol. .. The Y79 cells were transfected with plasmid mRuby2-N1 (empty vector, OE-NC, cat. no. 54614; Addgene, Watertown, MA, USA) or plasmid EndophilinA1-mRuby2 (overexpression SH3GL2, OE-SH3GL2, cat. no. 171945; Addgene) by Lipofectamine 3000 transfection reagent (Invitrogen) according to the manufacturer's protocol. ..



    Similar Products

    mruby2 n1  (Addgene inc)
    93
    Addgene inc mruby2 n1
    Mruby2 N1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+mruby2+n1/mRuby2-N1+(Plasmid+%2354614)/us12351877-394-25-31
    Average 93 stars, based on 1 article reviews
    mruby2 n1 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    mruby2  (Addgene inc)
    94
    Addgene inc mruby2
    Comparison of in‐gel fluorescence (IGF) of tagRFP‐T‐, <t>mRuby2‐,</t> mCherry‐, and mKO‐κ‐tagged proteins. (a) Yeast expressing Bmh1‐tagRFP‐T, Bmh1‐mRuby2, Bmh1‐mCherry, or Bmh1‐mKO‐κ were lysed in native conditions, samples were resuspended in LDS sample buffer and incubated for 5 min at the indicated temperatures. After migration on a commercial precast 4%–20%TGX gel (Bio‐Rad), gels were imaged for red fluorescence using a Typhoon or a Chemidoc MP, and total proteins were visualized by the stain‐free technology on a Chemidoc MP. Proteins were then transferred to a nitrocellulose membrane and immunoblotted with anti‐Bmh1 antibodies and then with anti‐rabbit antibodies coupled to HRP, and revealed by chemiluminescence on a Chemidoc MP. * indicates the fluorescent species. (b) Yeast expressing Bmh1‐mCherry were lysed in native conditions, samples were resuspended in LDS sample buffer and incubated for 5 min at the indicated temperatures in a gradient thermocycler. After migration on a commercial precast 4–20% TGX gel (Bio‐Rad), gels were imaged for red fluorescence using a Typhoon, and total proteins were visualized by the stain‐free technology on a Chemidoc. Proteins were then transferred to a nitrocellulose membrane and immunoblotted with anti‐Bmh1 antibodies and then with anti‐rabbit antibodies coupled to HRP, and revealed by chemiluminescence on a Chemidoc MP. (c) Same as (b) on lysates of yeast expressing Bmh1‐mKO‐κ. (d) Quantification of the fluorescence signal as a function of the denaturation temperature for mCherry‐tagged and mKO‐κ‐tagged Bmh1 ( n = 3; ±SD). Solid line: sigmoidal fit of the data (GraphPad Prism), dotted line: 95% confidence interval of the fit. HRP, horseradish peroxidase; LDS, lithium dodecyl sulfate.
    Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+mruby2+n1/mRuby2-N1+2xMyc+(Plasmid+%23118981)/pmc11808229-320-6-22
    Average 94 stars, based on 1 article reviews
    mruby2 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    plasmid mruby2 n1  (Addgene inc)
    93
    Addgene inc plasmid mruby2 n1
    Comparison of in‐gel fluorescence (IGF) of tagRFP‐T‐, <t>mRuby2‐,</t> mCherry‐, and mKO‐κ‐tagged proteins. (a) Yeast expressing Bmh1‐tagRFP‐T, Bmh1‐mRuby2, Bmh1‐mCherry, or Bmh1‐mKO‐κ were lysed in native conditions, samples were resuspended in LDS sample buffer and incubated for 5 min at the indicated temperatures. After migration on a commercial precast 4%–20%TGX gel (Bio‐Rad), gels were imaged for red fluorescence using a Typhoon or a Chemidoc MP, and total proteins were visualized by the stain‐free technology on a Chemidoc MP. Proteins were then transferred to a nitrocellulose membrane and immunoblotted with anti‐Bmh1 antibodies and then with anti‐rabbit antibodies coupled to HRP, and revealed by chemiluminescence on a Chemidoc MP. * indicates the fluorescent species. (b) Yeast expressing Bmh1‐mCherry were lysed in native conditions, samples were resuspended in LDS sample buffer and incubated for 5 min at the indicated temperatures in a gradient thermocycler. After migration on a commercial precast 4–20% TGX gel (Bio‐Rad), gels were imaged for red fluorescence using a Typhoon, and total proteins were visualized by the stain‐free technology on a Chemidoc. Proteins were then transferred to a nitrocellulose membrane and immunoblotted with anti‐Bmh1 antibodies and then with anti‐rabbit antibodies coupled to HRP, and revealed by chemiluminescence on a Chemidoc MP. (c) Same as (b) on lysates of yeast expressing Bmh1‐mKO‐κ. (d) Quantification of the fluorescence signal as a function of the denaturation temperature for mCherry‐tagged and mKO‐κ‐tagged Bmh1 ( n = 3; ±SD). Solid line: sigmoidal fit of the data (GraphPad Prism), dotted line: 95% confidence interval of the fit. HRP, horseradish peroxidase; LDS, lithium dodecyl sulfate.
    Plasmid Mruby2 N1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+mruby2+n1/mRuby2-N1+(Plasmid+%2354614)/pm36315123-92-6-14
    Average 93 stars, based on 1 article reviews
    plasmid mruby2 n1 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Comparison of in‐gel fluorescence (IGF) of tagRFP‐T‐, mRuby2‐, mCherry‐, and mKO‐κ‐tagged proteins. (a) Yeast expressing Bmh1‐tagRFP‐T, Bmh1‐mRuby2, Bmh1‐mCherry, or Bmh1‐mKO‐κ were lysed in native conditions, samples were resuspended in LDS sample buffer and incubated for 5 min at the indicated temperatures. After migration on a commercial precast 4%–20%TGX gel (Bio‐Rad), gels were imaged for red fluorescence using a Typhoon or a Chemidoc MP, and total proteins were visualized by the stain‐free technology on a Chemidoc MP. Proteins were then transferred to a nitrocellulose membrane and immunoblotted with anti‐Bmh1 antibodies and then with anti‐rabbit antibodies coupled to HRP, and revealed by chemiluminescence on a Chemidoc MP. * indicates the fluorescent species. (b) Yeast expressing Bmh1‐mCherry were lysed in native conditions, samples were resuspended in LDS sample buffer and incubated for 5 min at the indicated temperatures in a gradient thermocycler. After migration on a commercial precast 4–20% TGX gel (Bio‐Rad), gels were imaged for red fluorescence using a Typhoon, and total proteins were visualized by the stain‐free technology on a Chemidoc. Proteins were then transferred to a nitrocellulose membrane and immunoblotted with anti‐Bmh1 antibodies and then with anti‐rabbit antibodies coupled to HRP, and revealed by chemiluminescence on a Chemidoc MP. (c) Same as (b) on lysates of yeast expressing Bmh1‐mKO‐κ. (d) Quantification of the fluorescence signal as a function of the denaturation temperature for mCherry‐tagged and mKO‐κ‐tagged Bmh1 ( n = 3; ±SD). Solid line: sigmoidal fit of the data (GraphPad Prism), dotted line: 95% confidence interval of the fit. HRP, horseradish peroxidase; LDS, lithium dodecyl sulfate.

    Journal: Biology of the Cell

    Article Title: Direct observation of fluorescent proteins in gels: A rapid, cost‐efficient, and quantitative alternative to immunoblotting

    doi: 10.1111/boc.202400161

    Figure Lengend Snippet: Comparison of in‐gel fluorescence (IGF) of tagRFP‐T‐, mRuby2‐, mCherry‐, and mKO‐κ‐tagged proteins. (a) Yeast expressing Bmh1‐tagRFP‐T, Bmh1‐mRuby2, Bmh1‐mCherry, or Bmh1‐mKO‐κ were lysed in native conditions, samples were resuspended in LDS sample buffer and incubated for 5 min at the indicated temperatures. After migration on a commercial precast 4%–20%TGX gel (Bio‐Rad), gels were imaged for red fluorescence using a Typhoon or a Chemidoc MP, and total proteins were visualized by the stain‐free technology on a Chemidoc MP. Proteins were then transferred to a nitrocellulose membrane and immunoblotted with anti‐Bmh1 antibodies and then with anti‐rabbit antibodies coupled to HRP, and revealed by chemiluminescence on a Chemidoc MP. * indicates the fluorescent species. (b) Yeast expressing Bmh1‐mCherry were lysed in native conditions, samples were resuspended in LDS sample buffer and incubated for 5 min at the indicated temperatures in a gradient thermocycler. After migration on a commercial precast 4–20% TGX gel (Bio‐Rad), gels were imaged for red fluorescence using a Typhoon, and total proteins were visualized by the stain‐free technology on a Chemidoc. Proteins were then transferred to a nitrocellulose membrane and immunoblotted with anti‐Bmh1 antibodies and then with anti‐rabbit antibodies coupled to HRP, and revealed by chemiluminescence on a Chemidoc MP. (c) Same as (b) on lysates of yeast expressing Bmh1‐mKO‐κ. (d) Quantification of the fluorescence signal as a function of the denaturation temperature for mCherry‐tagged and mKO‐κ‐tagged Bmh1 ( n = 3; ±SD). Solid line: sigmoidal fit of the data (GraphPad Prism), dotted line: 95% confidence interval of the fit. HRP, horseradish peroxidase; LDS, lithium dodecyl sulfate.

    Article Snippet: Tagging with yeast codon‐optimized Tag‐RFP‐T or mRuby2 was achieved using the pFa6‐link‐yoTag‐RFP‐T‐CaURA3 and pFA6a‐link‐yomRuby2‐SpHIS5, respectively (gifts from Kurt Thorn and Wendell Lim, Addgene plasmids #44877 and #44843).

    Techniques: Comparison, Fluorescence, Expressing, Incubation, Migration, Staining, Membrane