plasmid egfp-centrin2 (Promega)
90
Structured Review
Promega
plasmid egfp-centrin2
Plasmid Egfp Centrin2, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+egfp-centrin2/plasmid+egfp+centrin2/pmc06864509-146-72-81
Average 90 stars, based on 1 article reviews
Plasmid Egfp Centrin2, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+egfp-centrin2/plasmid+egfp+centrin2/pmc06864509-146-72-81
Average 90 stars, based on 1 article reviews
plasmid egfp-centrin2 - by Bioz Stars,
2026-09
90/100 stars
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Incubation:Article Title: Scalable and Isotropic Expansion of Tissues with Simply Tunable Expansion Ratio Article Snippet: .. HeLa cells were then seeded into the wells with the density of 1 00 000 cells per well and incubated for 24 h at 37 °C in DMEM containing 10% v/v FBS and 0.01% w/v kanamycin at 37 °C in the presence of 5% CO 2 . eGFP‐centrin‐expressing HeLa cells were prepared by transfection of HeLa cells (2.4 × 10 5 cells on a 60 mm dish) with 2.5 μg of the plasmid (eGFP‐Centrin2, Accession number NM_004344.2, pLVX‐IRES‐Puro) using Fugene HD (Promega, E2311). .. One day after transfection, the cells were transferred to a 100 mm dish and treated with 1 mg mL −1 puromycin (Millipor sigma, 540222) for 2–3 weeks and then monoclonal cell lines were established with the dilution cloning method. Transfection:Article Title: Scalable and Isotropic Expansion of Tissues with Simply Tunable Expansion Ratio Article Snippet: .. HeLa cells were then seeded into the wells with the density of 1 00 000 cells per well and incubated for 24 h at 37 °C in DMEM containing 10% v/v FBS and 0.01% w/v kanamycin at 37 °C in the presence of 5% CO 2 . eGFP‐centrin‐expressing HeLa cells were prepared by transfection of HeLa cells (2.4 × 10 5 cells on a 60 mm dish) with 2.5 μg of the plasmid (eGFP‐Centrin2, Accession number NM_004344.2, pLVX‐IRES‐Puro) using Fugene HD (Promega, E2311). .. One day after transfection, the cells were transferred to a 100 mm dish and treated with 1 mg mL −1 puromycin (Millipor sigma, 540222) for 2–3 weeks and then monoclonal cell lines were established with the dilution cloning method. Plasmid Preparation:Article Title: Scalable and Isotropic Expansion of Tissues with Simply Tunable Expansion Ratio Article Snippet: .. HeLa cells were then seeded into the wells with the density of 1 00 000 cells per well and incubated for 24 h at 37 °C in DMEM containing 10% v/v FBS and 0.01% w/v kanamycin at 37 °C in the presence of 5% CO 2 . eGFP‐centrin‐expressing HeLa cells were prepared by transfection of HeLa cells (2.4 × 10 5 cells on a 60 mm dish) with 2.5 μg of the plasmid (eGFP‐Centrin2, Accession number NM_004344.2, pLVX‐IRES‐Puro) using Fugene HD (Promega, E2311). .. One day after transfection, the cells were transferred to a 100 mm dish and treated with 1 mg mL −1 puromycin (Millipor sigma, 540222) for 2–3 weeks and then monoclonal cell lines were established with the dilution cloning method. |
