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human pericytes  (PromoCell)


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    PromoCell human pericytes
    Human Pericytes, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/placenta+pericytes/Human+Pericytes+from+Placenta/pm41650956-926-2-7
    Average 95 stars, based on 96 article reviews
    human pericytes - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Pericyte plasticity - comparative investigation of the angiogenic and multilineage potential of pericytes from different human tissues.
    Article Snippet: .. Cell culture of retinal and placenta pericytes and HUVEC Two different commercially available pericyte types have been investigated as a source of mature pericytes: human primary retinal pericytes (R-PC, Cell Systems, Kirkland, WA, USA) and human primary placental-derived pericytes (PL-PC, Promocell, Heidelberg, Germany). .. Pericytes were cultured in pericyte growth medium (PGM, Promocell) at a seeding density of 3,000 cells/cm2 and subcultured upon 80 % confluency.

    Recombinant:

    Article Title: Placental pericytes and cytomegalovirus infectivity: implications for HCMV placental pathology and congenital disease
    Article Snippet: .. All cell types supported lytic replication of a HCMV-GFP recombinant virus, as demonstrated by phase/fluorescent imaging ( ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 A Placenta pericytes from Promocell cultivated in PM medium from ScienCell were allowed to develop as monolayers in chamber slides. ..

    Virus:

    Article Title: Placental pericytes and cytomegalovirus infectivity: implications for HCMV placental pathology and congenital disease
    Article Snippet: .. All cell types supported lytic replication of a HCMV-GFP recombinant virus, as demonstrated by phase/fluorescent imaging ( ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 A Placenta pericytes from Promocell cultivated in PM medium from ScienCell were allowed to develop as monolayers in chamber slides. ..

    Imaging:

    Article Title: Placental pericytes and cytomegalovirus infectivity: implications for HCMV placental pathology and congenital disease
    Article Snippet: .. All cell types supported lytic replication of a HCMV-GFP recombinant virus, as demonstrated by phase/fluorescent imaging ( ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 A Placenta pericytes from Promocell cultivated in PM medium from ScienCell were allowed to develop as monolayers in chamber slides. ..



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    TGF β -1 signalling via canonical receptor ALK5 in placental <t>pericytes.</t> ( a ) Representative image of ALK5/TGF β R1 expression (ICC/IF; green), WGA plasma membrane staining (red), nuclear stain (blue). Scale bar 150 μm; ( b ) RT-PCR analysis of Type I ALK5 TGFBR1 (91 bp) and Type II receptor, TGFBR2 (99 bp) in placental pericytes (n = 3); ( c ) pSMAD2 (52kD) and GAPDH (37kD) Western Blot. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( d ) Western Blot densitometry analysis; one-way ANOVA. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).
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    TGF β -1 signalling via canonical receptor ALK5 in placental <t>pericytes.</t> ( a ) Representative image of ALK5/TGF β R1 expression (ICC/IF; green), WGA plasma membrane staining (red), nuclear stain (blue). Scale bar 150 μm; ( b ) RT-PCR analysis of Type I ALK5 TGFBR1 (91 bp) and Type II receptor, TGFBR2 (99 bp) in placental pericytes (n = 3); ( c ) pSMAD2 (52kD) and GAPDH (37kD) Western Blot. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( d ) Western Blot densitometry analysis; one-way ANOVA. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).
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    Image Search Results


    TGF β -1 signalling via canonical receptor ALK5 in placental pericytes. ( a ) Representative image of ALK5/TGF β R1 expression (ICC/IF; green), WGA plasma membrane staining (red), nuclear stain (blue). Scale bar 150 μm; ( b ) RT-PCR analysis of Type I ALK5 TGFBR1 (91 bp) and Type II receptor, TGFBR2 (99 bp) in placental pericytes (n = 3); ( c ) pSMAD2 (52kD) and GAPDH (37kD) Western Blot. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( d ) Western Blot densitometry analysis; one-way ANOVA. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

    Journal: Scientific Reports

    Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

    doi: 10.1038/s41598-025-20432-9

    Figure Lengend Snippet: TGF β -1 signalling via canonical receptor ALK5 in placental pericytes. ( a ) Representative image of ALK5/TGF β R1 expression (ICC/IF; green), WGA plasma membrane staining (red), nuclear stain (blue). Scale bar 150 μm; ( b ) RT-PCR analysis of Type I ALK5 TGFBR1 (91 bp) and Type II receptor, TGFBR2 (99 bp) in placental pericytes (n = 3); ( c ) pSMAD2 (52kD) and GAPDH (37kD) Western Blot. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( d ) Western Blot densitometry analysis; one-way ANOVA. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

    Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

    Techniques: Expressing, Clinical Proteomics, Membrane, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

    ELISA analysis of angiogenic factors secreted from placental pericytes. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in; ( a ) VEGF secretion (Kruskal–Wallis analysis); ( b ) MMP-2 secretion (one-way ANOVA). ( c ) ANG-1 secretion (one-way ANOVA). ELISA results normalized to cell number. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

    Journal: Scientific Reports

    Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

    doi: 10.1038/s41598-025-20432-9

    Figure Lengend Snippet: ELISA analysis of angiogenic factors secreted from placental pericytes. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in; ( a ) VEGF secretion (Kruskal–Wallis analysis); ( b ) MMP-2 secretion (one-way ANOVA). ( c ) ANG-1 secretion (one-way ANOVA). ELISA results normalized to cell number. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

    Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

    Techniques: Enzyme-linked Immunosorbent Assay, Control

    Collagen IV, fibronectin and laminin staining in placental pericytes. Representative ICC/IF images (VEH-treated placental pericytes); ( a ) Collagen IV (red), nuclear stain (blue); data expressed as area and mean fluorescent intensity (MFI); one-way ANOVA, normalized to cell number; ( b ) Fibronectin (red), nuclear stain (blue), data expressed as area and MFI; one-way ANOVA, normalized to cell number; ( c ) Laminin (red), nuclear stain (blue); data expressed as area; Kruskal–Wallis analysis and MFI; one-way ANOVA, normalized to cell number. Scale Bars = 150 μm. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

    Journal: Scientific Reports

    Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

    doi: 10.1038/s41598-025-20432-9

    Figure Lengend Snippet: Collagen IV, fibronectin and laminin staining in placental pericytes. Representative ICC/IF images (VEH-treated placental pericytes); ( a ) Collagen IV (red), nuclear stain (blue); data expressed as area and mean fluorescent intensity (MFI); one-way ANOVA, normalized to cell number; ( b ) Fibronectin (red), nuclear stain (blue), data expressed as area and MFI; one-way ANOVA, normalized to cell number; ( c ) Laminin (red), nuclear stain (blue); data expressed as area; Kruskal–Wallis analysis and MFI; one-way ANOVA, normalized to cell number. Scale Bars = 150 μm. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

    Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

    Techniques: Staining

    Placental pericyte secretion of inflammatory factors. Analysis of inflammatory secretion (ELISA). Treatment groups: VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( a ) IL-6 secretion; one-way ANOVA; ( b ) MCP-1 secretion; one-way ANOVA; ( c ) sVCAM-1 secretion; one-way ANOVA; ( d ) CX3CL1 secretion; one-way ANOVA. ( e ) IL-8 secretion; Kruskal–Wallis analysis. Data (n = 3); stars indicate statistical significance between treatment groups ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

    Journal: Scientific Reports

    Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

    doi: 10.1038/s41598-025-20432-9

    Figure Lengend Snippet: Placental pericyte secretion of inflammatory factors. Analysis of inflammatory secretion (ELISA). Treatment groups: VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( a ) IL-6 secretion; one-way ANOVA; ( b ) MCP-1 secretion; one-way ANOVA; ( c ) sVCAM-1 secretion; one-way ANOVA; ( d ) CX3CL1 secretion; one-way ANOVA. ( e ) IL-8 secretion; Kruskal–Wallis analysis. Data (n = 3); stars indicate statistical significance between treatment groups ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

    Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

    Techniques: Enzyme-linked Immunosorbent Assay, Control

    Alternate TGF β -1 signalling pathway in placental pericytes: Type I receptor ACVRL1 (ALK1, 94 bp) expression in placental pericytes, as assessed by RT-PCR; original image shown.

    Journal: Scientific Reports

    Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

    doi: 10.1038/s41598-025-20432-9

    Figure Lengend Snippet: Alternate TGF β -1 signalling pathway in placental pericytes: Type I receptor ACVRL1 (ALK1, 94 bp) expression in placental pericytes, as assessed by RT-PCR; original image shown.

    Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    Phagocytosis capacity of placental pericytes. Treatment groups: VEH control and TGF β -1 (10 ng/mL) with and without FluoSphere incubation; ( a ) Representative flow cytometry histograms to identify the percentage of the population with and without phagocytosed FluoSpheres based on fluorescence; ( b ) Analysis of phagocytosis (Mann–Whitney). Data (n = 3) expressed as mean ± SEM. Stars indicate statistical significance, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001; c Fluorescent and phase contrast images of pericyte phagocytosis, nuclei (blue), FluoSphere (green). Yellow arrows identify pericytes with phagocytosed beads, and red arrows identify beads that have not been phagocytosed. Scale bar = 150 μm.

    Journal: Scientific Reports

    Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

    doi: 10.1038/s41598-025-20432-9

    Figure Lengend Snippet: Phagocytosis capacity of placental pericytes. Treatment groups: VEH control and TGF β -1 (10 ng/mL) with and without FluoSphere incubation; ( a ) Representative flow cytometry histograms to identify the percentage of the population with and without phagocytosed FluoSpheres based on fluorescence; ( b ) Analysis of phagocytosis (Mann–Whitney). Data (n = 3) expressed as mean ± SEM. Stars indicate statistical significance, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001; c Fluorescent and phase contrast images of pericyte phagocytosis, nuclei (blue), FluoSphere (green). Yellow arrows identify pericytes with phagocytosed beads, and red arrows identify beads that have not been phagocytosed. Scale bar = 150 μm.

    Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

    Techniques: Control, Incubation, Flow Cytometry, Fluorescence, MANN-WHITNEY