Journal: PLOS One

Article Title: Lactic acid regulates antitumor immunity in canine invasive urothelial carcinoma

doi: 10.1371/journal.pone.0332825

Figure Lengend Snippet: (A) Cell proliferation assay results using NSAIDs (aspirin, piroxicam, diclofenac, and robenacoxib). The iUC cell lines were seeded in triplicate in the presence of NSAIDs at the indicated concentrations for 48 h, and cell proliferation rates were determined using the Cell Counting Kit-8 assay. The proliferation rate of NSAID-treated cells at each concentration is expressed relative to DMSO-treated control cells, set at 100%. The results were evaluated in three independent experiments. Mean ± SD is shown. Statistically significant differences compared to the DMSO-treated control group were first observed at a certain concentration, and all higher concentrations were also considered significantly different. Dunnett test, p < 0.05. These concentrations are indicated in the figure: *aspirin, † piroxicam, ‡ diclofenac, § robenacoxib. (B) Results of extracellular lactic acid levels using NSAIDs. The iUC cell lines were seeded in the presence of NSAIDs at concentrations of 100 or 200 μM for 24 h, and extracellular lactic acid levels was measured using a lactic acid assay kit. The results were evaluated in three independent experiments. Mean ± SD is shown. Tukey-Kramer test, * p < 0.05, ** p < 0.01. NS, not significant.

Article Snippet: The cells were treated with the indicated concentrations (6.25–200 μM) of NSAIDs, including aspirin, piroxicam, diclofenac (all from Selleck, Houston, USA), and robenacoxib (LGC, Middlesex, UK), for 48 hours.

Techniques: Proliferation Assay, Cell Counting, Concentration Assay, Control, Acid Assay

Journal: Gut Microbes

Article Title: Human gut commensal Alistipes timonensis modulates the host lipidome and delivers anti-inflammatory outer membrane vesicles to suppress colitis in an Il10 -deficient mouse model

doi: 10.1080/19490976.2025.2517380

Figure Lengend Snippet: A. timonensis colonization delays piroxicam-accelerated colitis development in IL10 KO mice. (a) Representative H&E images of uncolonized (uncol.) and A. timonensis -colonized ( A. tim .) mouse colons showing less inflammation in colonized mice. One-sided student’s t -test confirmed significantly decreased gross pathology and histology scores in uncol. ( n = 9 mice) compared to A. tim . ( n = 9 mice) mice. (b-e) The expression of pro-inflammatory factors Il1b , Tnfa , Il6 , and Nos2 were significantly decreased in the distal colons of A. timonensis -colonized ( A. tim., n = 9 mice) mice compared to uncolonized (Uncol., n = 9 mice) mice, supporting that Alistipes colonization mediated a suppressive effect against colitis progression. Significance was determined using two-sided student’s t -test. For all p -values, * 0.05 > p > 0.0 and **** 0.0001 > p .

Article Snippet: Briefly, on day 0, all mice were switched to a diet supplemented with 100 ppm of piroxicam (Cayman Chemical 13,368; Inotiv Teklad, TD.210442).

Techniques: Expressing