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Image Search Results
Journal: Redox Biology
Article Title: EFHD2 contributes to non-small cell lung cancer cisplatin resistance by the activation of NOX4-ROS-ABCC1 axis
doi: 10.1016/j.redox.2020.101571
Figure Lengend Snippet: Ibuprofen suppresses EFHD2 and sensitizes lung cancer cells to cisplatin. A) NSAIDs, aspirin 500 μM, diclofenac 40 μM, ibuprofen 600 μM, ketorolac 10 μM, mefenamic acid 2 μM, piroxicam 10 μM, and sulindac 20 μM, were used to treat both H1299 and F4 cells for 24 h. EFHD2 levels were determined by Western blot assay. B) H1299 and F4 cells were treated with indicated dose of ibuprofen. The relative EFHD2 levels in each sample were normalized to the control cell. β-actin, loading control. C) After 24 h pretreatment with indicated doses of ibuprofen, migration ability of H1299 and F4 cells was analyzed by real time quantification using the IncuCyte system. D) After 24 h pretreatment with indicated doses of ibuprofen, invasion ability of H1299 and F4 cells was analyzed by matrigel transwell system. Invaded cell number was normalized with cell viability and the relative invasion was normalized with the control cells. E) EFHD2-KD and the control H1299 cells and F) EFHD2-OE and the control A549 cells were pretreated with or without 600 μM ibuprofen for 24 h, and then indicated doses of cisplatin were added into culture media for another 24 h. Cell viability were determined by MTT assay. ★★ , <0.01.
Article Snippet: I4883) were purchased from the Sigma-Aldrich; bafilomycin A1 (Baf-A1; Cat.No. sc-201550), aspirin (Cat.No. sc-202471), diclofenac (Cat.No. sc-357332), ketorolac (Cat.No. sc-205360), mefenamic acid (Cat.No. sc-205380),
Techniques: Western Blot, Control, Migration, MTT Assay