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Yeasen Biotechnology picogreen dsdna quantitation
Picogreen Dsdna Quantitation, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/picogreen/dsdna+picogreen+quantitation+reagent/10__1096_slash_fj__202601242r-43-0-7
Average 86 stars, based on 1 article reviews
picogreen dsdna quantitation - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Staining:

Article Title: P2X7 receptor-mediated cGAS-STING pathway activation underlies chronic stress-induced depressive-like behaviors.
Article Snippet: .. To stain dsDNA and mitochondria, cells were incubated with 250 nM PK Mito Orange (a mitochondrial label, #PKMO-1, Genvivo Biotech, China) and 500 ng/ml Picogreen (#12641ES, Yeasen Biotechnology, China) in medium at 37 °C for 20 min, washed with PBS for 30 min. A Nikon N-SIM Structured Illumination microscope (Nikon, Japan) was used to obtain images. .. ImageJ software was used to observe colocalization between dsDNA and mitochondria.

Article Title: Identification of mitochondrial-related genes to evaluate the immune infiltration and prognosis of lung adenocarcinoma
Article Snippet: .. Cells were stained with Mitotracker (Invitrogen, Waltham, MA, USA), PicoGreen (Yeasen, Shanghai, China), and Hoechst 33342 (Yeasen, Shanghai, China) at final concentrations of 200 nM for 30 min, 3 μL/mL for 1 h, and 0.5 μg/mL for 10 min, respectively. ..

Article Title: P2X7 receptor-mediated cGAS-STING pathway activation underlies chronic stress-induced depressive-like behaviors
Article Snippet: .. To stain dsDNA and mitochondria, cells were incubated with 250 nM PK Mito Orange (a mitochondrial label, #PKMO-1, Genvivo Biotech, China) and 500 ng/ml Picogreen (#12641ES, Yeasen Biotechnology, China) in medium at 37 °C for 20 min, washed with PBS for 30 min. A Nikon N-SIM Structured Illumination microscope (Nikon, Japan) was used to obtain images. .. ImageJ software was used to observe colocalization between dsDNA and mitochondria.

Article Title: HSP90α facilitates the dephosphorylation of DRP1 at the Ser637 residue, thereby promoting NLRP3 inflammasome activation and exacerbating brain injury in sepsis.
Article Snippet: Sepsis-associated encephalopathy (SAE) is a common and serious complication of sepsis, characterized by neuroinflammation and cognitive dysfunction, yet its molecular mechanisms remain unclear.. This study aimed to investigate the role of heat shock protein 90α (HSP90α) in microglial NLRP3 inflammasome activation and cognitive impairment in SAE, and to explore the therapeutic potential of a small molecule compound, nicotinamide N-oxide (NAMO).. SAE models were established using cecal ligation and puncture (CLP) in mice and LPS/ ATP stimulated primary microglial cells/BV-2 cells.

Incubation:

Article Title: P2X7 receptor-mediated cGAS-STING pathway activation underlies chronic stress-induced depressive-like behaviors.
Article Snippet: .. To stain dsDNA and mitochondria, cells were incubated with 250 nM PK Mito Orange (a mitochondrial label, #PKMO-1, Genvivo Biotech, China) and 500 ng/ml Picogreen (#12641ES, Yeasen Biotechnology, China) in medium at 37 °C for 20 min, washed with PBS for 30 min. A Nikon N-SIM Structured Illumination microscope (Nikon, Japan) was used to obtain images. .. ImageJ software was used to observe colocalization between dsDNA and mitochondria.

Article Title: P2X7 receptor-mediated cGAS-STING pathway activation underlies chronic stress-induced depressive-like behaviors
Article Snippet: .. To stain dsDNA and mitochondria, cells were incubated with 250 nM PK Mito Orange (a mitochondrial label, #PKMO-1, Genvivo Biotech, China) and 500 ng/ml Picogreen (#12641ES, Yeasen Biotechnology, China) in medium at 37 °C for 20 min, washed with PBS for 30 min. A Nikon N-SIM Structured Illumination microscope (Nikon, Japan) was used to obtain images. .. ImageJ software was used to observe colocalization between dsDNA and mitochondria.

Article Title: The low-dose CHK1 inhibitor prexasertib triggers VDAC1 dephosphorylation to activate mtDNA-STING signaling and synergize immunotherapy.
Article Snippet: In brief Fu et al. find that low-dose prexasertib, a CHK1 inhibitor, enhances tumor sensitivity while mitigating on-target toxicity during immunotherapy through mtDNA-mediated STING pathway activation.. Mechanistically, prexasertib at therapeutic doses blocks Nek1-mediated phosphorylation of VDAC1 at the threonine 107 residue, showing a phosphorylation-dependent regulatory mechanism of mitochondrial-nuclear crosstalk.

Article Title: HSP90α facilitates the dephosphorylation of DRP1 at the Ser637 residue, thereby promoting NLRP3 inflammasome activation and exacerbating brain injury in sepsis.
Article Snippet: Sepsis-associated encephalopathy (SAE) is a common and serious complication of sepsis, characterized by neuroinflammation and cognitive dysfunction, yet its molecular mechanisms remain unclear.. This study aimed to investigate the role of heat shock protein 90α (HSP90α) in microglial NLRP3 inflammasome activation and cognitive impairment in SAE, and to explore the therapeutic potential of a small molecule compound, nicotinamide N-oxide (NAMO).. SAE models were established using cecal ligation and puncture (CLP) in mice and LPS/ ATP stimulated primary microglial cells/BV-2 cells.

Microscopy:

Article Title: P2X7 receptor-mediated cGAS-STING pathway activation underlies chronic stress-induced depressive-like behaviors.
Article Snippet: .. To stain dsDNA and mitochondria, cells were incubated with 250 nM PK Mito Orange (a mitochondrial label, #PKMO-1, Genvivo Biotech, China) and 500 ng/ml Picogreen (#12641ES, Yeasen Biotechnology, China) in medium at 37 °C for 20 min, washed with PBS for 30 min. A Nikon N-SIM Structured Illumination microscope (Nikon, Japan) was used to obtain images. .. ImageJ software was used to observe colocalization between dsDNA and mitochondria.

Article Title: P2X7 receptor-mediated cGAS-STING pathway activation underlies chronic stress-induced depressive-like behaviors
Article Snippet: .. To stain dsDNA and mitochondria, cells were incubated with 250 nM PK Mito Orange (a mitochondrial label, #PKMO-1, Genvivo Biotech, China) and 500 ng/ml Picogreen (#12641ES, Yeasen Biotechnology, China) in medium at 37 °C for 20 min, washed with PBS for 30 min. A Nikon N-SIM Structured Illumination microscope (Nikon, Japan) was used to obtain images. .. ImageJ software was used to observe colocalization between dsDNA and mitochondria.

Article Title: HSP90α facilitates the dephosphorylation of DRP1 at the Ser637 residue, thereby promoting NLRP3 inflammasome activation and exacerbating brain injury in sepsis.
Article Snippet: Sepsis-associated encephalopathy (SAE) is a common and serious complication of sepsis, characterized by neuroinflammation and cognitive dysfunction, yet its molecular mechanisms remain unclear.. This study aimed to investigate the role of heat shock protein 90α (HSP90α) in microglial NLRP3 inflammasome activation and cognitive impairment in SAE, and to explore the therapeutic potential of a small molecule compound, nicotinamide N-oxide (NAMO).. SAE models were established using cecal ligation and puncture (CLP) in mice and LPS/ ATP stimulated primary microglial cells/BV-2 cells.

other:

Article Title: Fibroblast-derived exosomes induce macrophage pyroptosis to mediate orthodontic bone remodeling through the mtDNA/NLRP3 axis.
Article Snippet: Following three washes with PBS, the cells were immediately imaged via confocal microscopy (FV3000, Olympus, Japan).

Labeling:

Article Title: Fibroblast-derived exosomes induce macrophage pyroptosis to mediate orthodontic bone remodeling through the mtDNA/NLRP3 axis.
Article Snippet: .. Tracking of exosome uptake by macrophages Exosome suspensions were dual labeled with PKH26 (MedChemExpress, USA, Cat. No. HY-D1451) and PicoGreen (Yeasen, China, Cat. No. 12641ES01) according to the manufacturers' recommended concentrations. .. Administration of exosomes The protein concentration of the isolated exosomes was quantified using a BCA protein assay (Solarbio, China, Cat. No. PC0020).

Fluorescence:

Article Title: HSP90α facilitates the dephosphorylation of DRP1 at the Ser637 residue, thereby promoting NLRP3 inflammasome activation and exacerbating brain injury in sepsis.
Article Snippet: Sepsis-associated encephalopathy (SAE) is a common and serious complication of sepsis, characterized by neuroinflammation and cognitive dysfunction, yet its molecular mechanisms remain unclear.. This study aimed to investigate the role of heat shock protein 90α (HSP90α) in microglial NLRP3 inflammasome activation and cognitive impairment in SAE, and to explore the therapeutic potential of a small molecule compound, nicotinamide N-oxide (NAMO).. SAE models were established using cecal ligation and puncture (CLP) in mice and LPS/ ATP stimulated primary microglial cells/BV-2 cells.



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The standard curve was generated by plotting the fluorescence intensity against the <t>dsDNA</t> concentration (ng/mL). Due to the wide dynamic range of the assay, both axes are presented with a break to clearly display the low-concentration region (0–50 ng/mL) and the high-concentration region (50–1,000 ng/mL) on a single graph. Data points represent the mean <t>PicoGreen</t> fluorescence from a single independent experiment, performed with at least three technical replicates per dsDNA concentration. The dsDNA concentrations shown are consistent with those listed in . Standard deviations (SD) were calculated for each concentration, and the coefficients of variation were consistently below 5% across all concentrations tested. The linear regression equation is y = 15.9x + 78.831 (R 2 = 1), where y is PicoGreen fluorescence intensity and x is dsDNA concentration in ng/mL. Unknown concentrations can be calculated as x = (y - 78.831)/15.92. The dsDNA detection range is 1–1,000 ng/mL.
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The standard curve was generated by plotting the fluorescence intensity against the <t>dsDNA</t> concentration (ng/mL). Due to the wide dynamic range of the assay, both axes are presented with a break to clearly display the low-concentration region (0–50 ng/mL) and the high-concentration region (50–1,000 ng/mL) on a single graph. Data points represent the mean <t>PicoGreen</t> fluorescence from a single independent experiment, performed with at least three technical replicates per dsDNA concentration. The dsDNA concentrations shown are consistent with those listed in . Standard deviations (SD) were calculated for each concentration, and the coefficients of variation were consistently below 5% across all concentrations tested. The linear regression equation is y = 15.9x + 78.831 (R 2 = 1), where y is PicoGreen fluorescence intensity and x is dsDNA concentration in ng/mL. Unknown concentrations can be calculated as x = (y - 78.831)/15.92. The dsDNA detection range is 1–1,000 ng/mL.
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Image Search Results


The standard curve was generated by plotting the fluorescence intensity against the dsDNA concentration (ng/mL). Due to the wide dynamic range of the assay, both axes are presented with a break to clearly display the low-concentration region (0–50 ng/mL) and the high-concentration region (50–1,000 ng/mL) on a single graph. Data points represent the mean PicoGreen fluorescence from a single independent experiment, performed with at least three technical replicates per dsDNA concentration. The dsDNA concentrations shown are consistent with those listed in . Standard deviations (SD) were calculated for each concentration, and the coefficients of variation were consistently below 5% across all concentrations tested. The linear regression equation is y = 15.9x + 78.831 (R 2 = 1), where y is PicoGreen fluorescence intensity and x is dsDNA concentration in ng/mL. Unknown concentrations can be calculated as x = (y - 78.831)/15.92. The dsDNA detection range is 1–1,000 ng/mL.

Journal: Bio-protocol

Article Title: A Cell-Based Protocol to Assess Manganese Content and Relative Transport Activity of Manganese Transporters

doi: 10.21769/BioProtoc.5680

Figure Lengend Snippet: The standard curve was generated by plotting the fluorescence intensity against the dsDNA concentration (ng/mL). Due to the wide dynamic range of the assay, both axes are presented with a break to clearly display the low-concentration region (0–50 ng/mL) and the high-concentration region (50–1,000 ng/mL) on a single graph. Data points represent the mean PicoGreen fluorescence from a single independent experiment, performed with at least three technical replicates per dsDNA concentration. The dsDNA concentrations shown are consistent with those listed in . Standard deviations (SD) were calculated for each concentration, and the coefficients of variation were consistently below 5% across all concentrations tested. The linear regression equation is y = 15.9x + 78.831 (R 2 = 1), where y is PicoGreen fluorescence intensity and x is dsDNA concentration in ng/mL. Unknown concentrations can be calculated as x = (y - 78.831)/15.92. The dsDNA detection range is 1–1,000 ng/mL.

Article Snippet: Picogreen dsDNA quantitation reagent (YEASEN, catalog number: 12641ES04) Western blotting 17.

Techniques: Generated, Fluorescence, Concentration Assay