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phosphate buffered saline dpbs  (Bio-Rad)


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    Bio-Rad phosphate buffered saline dpbs
    Phosphate Buffered Saline Dpbs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 2900 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphate+buffer+saline+pbs/Phosphate+Buffered+Saline+(PBS)/pmc13634633-83-17-36
    Average 96 stars, based on 2900 article reviews
    phosphate buffered saline dpbs - by Bioz Stars, 2026-10
    96/100 stars

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    Saline:

    Article Title: Pharmaceutical composition of S1PR modulators
    Article Snippet: The samples were subjected to SDS-polyacrylamide 10-12% (SDS-PAGE) gel electrophoresis and to Western blotting on Amersham Hybond p 0.45 PVDF membrane (GE Healthcare, Buckinghamshire, UK). .. The membranes, after sufficient washings with phosphate buffer saline PBS have been initially blocked with PBST buffer (150 mM NaCl, 0.05% Tween-20 and 20 mM Tris-HCl, pH 7.4) containing 5% of non-fat dry milk (Bio-Rad, Hercules, CA) for 1 hour at room temperature. ..

    Article Title: Immunological Response of Interleukin-6 on Cantang Hybrid Grouper (Ephinephelus sp.) By Induction Of Protein Brachionus sp.
    Article Snippet: .. The materials used in this study were Cantang Grouper (7-10 cm, ±15 g), Antimouse antibody IL-6, Heparin sodium, Freshwater and Marine, Brachionus sp., Whole Cell Protein Brachionus sp., Protein extract Brachionus sp. VNN positive grouper, aquadest, seawater, 70% alcohol, Phosphate Buffer Saline/PBS (bio-Rad), separating gel, stacking gel, lower gel buffer, upper gel buffer, PBS solution, T-acryl, ddH2O, Tetra Methyl Diamine (TEMED)(bio-Rad), ammonium persulfate, Tris (hydroxymethyl), HCl (Merck) pH=8.8 and 6.5, detergent Sodium Dodecyl Sulphate (SDS), and Comassie Brilliant Blue dye. ..

    Article Title: Apigenin, a natural flavonoid, promotes autophagy and ferroptosis in human endometrial carcinoma Ishikawa cells in vitro and in vivo
    Article Snippet: Apigenin, a natural flavonoid has been reported against a variety of cancer types.. However, it is unclear whether apigenin can promote autophagy and ferroptosis in Ishikawa cells.. There are few reports on the mechanism of apigenin on autophagy and ferroptosis of endometrial cancer Ishikawa cells.

    Article Title: Expression and purification of recombinant tilapia lake virus segment 4 protein and its in-vitro biological activity for potential use in vaccine development
    Article Snippet: The protein concentration in the supernatant was quantified using a bicinchoninic acid assay (Thermo Fisher Scientific, USA), and the recombinant TiLV S4 protein expression was analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). .. The proteins were then transferred to a nitrocellulose membrane, which was subsequently blocked in a blocking buffer comprising 5% (w/v) skim milk in phosphate buffer saline (PBS) at 4 °C for 16 h. The membrane was then washed three times with PBS containing 0.1% (v/v) Tween 20 (PBS-T) and incubated with a 1:4000 dilution of horseradish peroxidase (HRP)-conjugated anti-His antibody (Bio-Rad, USA) in the blocking buffer for 1 h. Following incubation, the membrane was washed three times with PBS-T and incubated with an enhanced chemiluminescence (ECL) substrate. .. The developed protein bands were observed using the iBrightTM CL1500 Imaging System (Invitrogen, USA).

    Article Title: Gastric mucosal CD8 + T RM cells are recruited through CXCR5-CXCL13 axis in Helicobacter pylori infected subjects.
    Article Snippet: Antigen retrieval was performed in a microwave oven using Tris-EDTA Antigen Retrieval Solution (C1038–2; Solarbio, CHN). .. For CD8+TRM cells staining, slides were blocked with 5 % Bovine Serum Albumin (BSA) in phosphate buffer saline (PBS) containing 0.5 % tween-20 (PBST) and then incubated with primary antibody against CD8 (MCA351GT, 1:50; Bio-Rad, USA), CD69 (sc-373799, 1:50; Santa Cruz Biotechnology, USA), and CD103 (ab129202, 1:1000; Abcam, USA) at 4 ◦C overnight. .. Slides were washed with PBST five times and incubated with Alexa Fluor 568 conjugated goat anti-rat IgG H&L (ab175476, 1:1000; Abcam), Alexa Fluor 488 conjugated goat antimouse IgG H&L (ab150113, 1:1000; Abcam) and Alexa Fluor 647 conjugated goat anti-rabbit IgG H&L (ab150079, 1:1000; Abcam) at room temperature for 1 h. After washing with PBST for five times, slides were counterstained with DAPI (C0065; Solarbio).

    Article Title: Manzamine A reduces androgen receptor transcription and synthesis by blocking E2F8-DNA interactions and effectively inhibits prostate tumor growth in mice.
    Article Snippet: .. On day 15, cells were washed with phosphate buffer saline (PBS), fixed in ice-cold Methanol for 15 min at 20 °C, and stained in QC Colloidal Coomassie blue (Cat. no. 1610803; BioRad, Hercules, CA, USA) overnight at room temperature and washed with water. .. Developed cell colonies were analyzed and counted using IMAGEJ software (National Institute of Health, Bethesda, MD, USA).

    Article Title: Manzamine A reduces androgen receptor transcription and synthesis by blocking E 2 F 8‐ DNA interactions and effectively inhibits prostate tumor growth in mice
    Article Snippet: .. On day 15, cells were washed with phosphate buffer saline (PBS), fixed in ice‐cold Methanol for 15 min at −20 °C, and stained in QC Colloidal Coomassie blue (Cat. no. 1610803; BioRad, Hercules, CA, USA) overnight at room temperature and washed with water. .. Developed cell colonies were analyzed and counted using imagej software (National Institute of Health, Bethesda, MD, USA).

    Staining:

    Article Title: Apigenin, a natural flavonoid, promotes autophagy and ferroptosis in human endometrial carcinoma Ishikawa cells in vitro and in vivo
    Article Snippet: Apigenin, a natural flavonoid has been reported against a variety of cancer types.. However, it is unclear whether apigenin can promote autophagy and ferroptosis in Ishikawa cells.. There are few reports on the mechanism of apigenin on autophagy and ferroptosis of endometrial cancer Ishikawa cells.

    Article Title: Gastric mucosal CD8 + T RM cells are recruited through CXCR5-CXCL13 axis in Helicobacter pylori infected subjects.
    Article Snippet: Antigen retrieval was performed in a microwave oven using Tris-EDTA Antigen Retrieval Solution (C1038–2; Solarbio, CHN). .. For CD8+TRM cells staining, slides were blocked with 5 % Bovine Serum Albumin (BSA) in phosphate buffer saline (PBS) containing 0.5 % tween-20 (PBST) and then incubated with primary antibody against CD8 (MCA351GT, 1:50; Bio-Rad, USA), CD69 (sc-373799, 1:50; Santa Cruz Biotechnology, USA), and CD103 (ab129202, 1:1000; Abcam, USA) at 4 ◦C overnight. .. Slides were washed with PBST five times and incubated with Alexa Fluor 568 conjugated goat anti-rat IgG H&L (ab175476, 1:1000; Abcam), Alexa Fluor 488 conjugated goat antimouse IgG H&L (ab150113, 1:1000; Abcam) and Alexa Fluor 647 conjugated goat anti-rabbit IgG H&L (ab150079, 1:1000; Abcam) at room temperature for 1 h. After washing with PBST for five times, slides were counterstained with DAPI (C0065; Solarbio).

    Article Title: Manzamine A reduces androgen receptor transcription and synthesis by blocking E2F8-DNA interactions and effectively inhibits prostate tumor growth in mice.
    Article Snippet: .. On day 15, cells were washed with phosphate buffer saline (PBS), fixed in ice-cold Methanol for 15 min at 20 °C, and stained in QC Colloidal Coomassie blue (Cat. no. 1610803; BioRad, Hercules, CA, USA) overnight at room temperature and washed with water. .. Developed cell colonies were analyzed and counted using IMAGEJ software (National Institute of Health, Bethesda, MD, USA).

    Article Title: Manzamine A reduces androgen receptor transcription and synthesis by blocking E 2 F 8‐ DNA interactions and effectively inhibits prostate tumor growth in mice
    Article Snippet: .. On day 15, cells were washed with phosphate buffer saline (PBS), fixed in ice‐cold Methanol for 15 min at −20 °C, and stained in QC Colloidal Coomassie blue (Cat. no. 1610803; BioRad, Hercules, CA, USA) overnight at room temperature and washed with water. .. Developed cell colonies were analyzed and counted using imagej software (National Institute of Health, Bethesda, MD, USA).

    Membrane:

    Article Title: Expression and purification of recombinant tilapia lake virus segment 4 protein and its in-vitro biological activity for potential use in vaccine development
    Article Snippet: The protein concentration in the supernatant was quantified using a bicinchoninic acid assay (Thermo Fisher Scientific, USA), and the recombinant TiLV S4 protein expression was analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). .. The proteins were then transferred to a nitrocellulose membrane, which was subsequently blocked in a blocking buffer comprising 5% (w/v) skim milk in phosphate buffer saline (PBS) at 4 °C for 16 h. The membrane was then washed three times with PBS containing 0.1% (v/v) Tween 20 (PBS-T) and incubated with a 1:4000 dilution of horseradish peroxidase (HRP)-conjugated anti-His antibody (Bio-Rad, USA) in the blocking buffer for 1 h. Following incubation, the membrane was washed three times with PBS-T and incubated with an enhanced chemiluminescence (ECL) substrate. .. The developed protein bands were observed using the iBrightTM CL1500 Imaging System (Invitrogen, USA).

    Blocking Assay:

    Article Title: Expression and purification of recombinant tilapia lake virus segment 4 protein and its in-vitro biological activity for potential use in vaccine development
    Article Snippet: The protein concentration in the supernatant was quantified using a bicinchoninic acid assay (Thermo Fisher Scientific, USA), and the recombinant TiLV S4 protein expression was analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). .. The proteins were then transferred to a nitrocellulose membrane, which was subsequently blocked in a blocking buffer comprising 5% (w/v) skim milk in phosphate buffer saline (PBS) at 4 °C for 16 h. The membrane was then washed three times with PBS containing 0.1% (v/v) Tween 20 (PBS-T) and incubated with a 1:4000 dilution of horseradish peroxidase (HRP)-conjugated anti-His antibody (Bio-Rad, USA) in the blocking buffer for 1 h. Following incubation, the membrane was washed three times with PBS-T and incubated with an enhanced chemiluminescence (ECL) substrate. .. The developed protein bands were observed using the iBrightTM CL1500 Imaging System (Invitrogen, USA).

    Incubation:

    Article Title: Expression and purification of recombinant tilapia lake virus segment 4 protein and its in-vitro biological activity for potential use in vaccine development
    Article Snippet: The protein concentration in the supernatant was quantified using a bicinchoninic acid assay (Thermo Fisher Scientific, USA), and the recombinant TiLV S4 protein expression was analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). .. The proteins were then transferred to a nitrocellulose membrane, which was subsequently blocked in a blocking buffer comprising 5% (w/v) skim milk in phosphate buffer saline (PBS) at 4 °C for 16 h. The membrane was then washed three times with PBS containing 0.1% (v/v) Tween 20 (PBS-T) and incubated with a 1:4000 dilution of horseradish peroxidase (HRP)-conjugated anti-His antibody (Bio-Rad, USA) in the blocking buffer for 1 h. Following incubation, the membrane was washed three times with PBS-T and incubated with an enhanced chemiluminescence (ECL) substrate. .. The developed protein bands were observed using the iBrightTM CL1500 Imaging System (Invitrogen, USA).

    Article Title: Gastric mucosal CD8 + T RM cells are recruited through CXCR5-CXCL13 axis in Helicobacter pylori infected subjects.
    Article Snippet: Antigen retrieval was performed in a microwave oven using Tris-EDTA Antigen Retrieval Solution (C1038–2; Solarbio, CHN). .. For CD8+TRM cells staining, slides were blocked with 5 % Bovine Serum Albumin (BSA) in phosphate buffer saline (PBS) containing 0.5 % tween-20 (PBST) and then incubated with primary antibody against CD8 (MCA351GT, 1:50; Bio-Rad, USA), CD69 (sc-373799, 1:50; Santa Cruz Biotechnology, USA), and CD103 (ab129202, 1:1000; Abcam, USA) at 4 ◦C overnight. .. Slides were washed with PBST five times and incubated with Alexa Fluor 568 conjugated goat anti-rat IgG H&L (ab175476, 1:1000; Abcam), Alexa Fluor 488 conjugated goat antimouse IgG H&L (ab150113, 1:1000; Abcam) and Alexa Fluor 647 conjugated goat anti-rabbit IgG H&L (ab150079, 1:1000; Abcam) at room temperature for 1 h. After washing with PBST for five times, slides were counterstained with DAPI (C0065; Solarbio).

    other:

    Article Title: Toxic, genotoxic, mutagenic, and bioaccumulative effects of metal mixture from settleable particulate matter on American bullfrog tadpoles (Lithobates catesbeianus).
    Article Snippet: Amphibians are more susceptible to environmental stressors than other vertebrates due to their semipermeable skin and physiological adaptations to living in very specific microhabitats.. Therefore, the aim of the present study was to investigate the effects of a metal mixture from settleable particulate matter (SePM) released from metallurgical industries on Lithobates catesbeianus tadpoles.. Endpoints analyzed included metal bioconcentration, morphological (biometrical indices), hematological parameters (hemoglobin and blood cell count), and erythrocyte DNA damage (genotoxicity and mutagenicity).



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    Methodological workflow from sample preparation to measuring the cellular response capacity (CRC). Following blood collection, samples are prepared for flow cytometric analysis either unstimulated (PBS as buffer control) or stimulated with an inflammatory cocktail (cocktail of N-formylmethionyl-leucyl-phenylalanine, platelet-activating factor, and tumor necrosis factor). The CRC is calculated as the ratio of median fluorescence intensity (MFI) between stimulated and unstimulated neutrophils. Three approaches, classic, simple, and kinetic CRC, offer distinct advantages and limitations based on technical aspects such as manual processing steps (e.g., centrifugation) and incubation time. n =13–14. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing patients with sepsis at all time points (shown in the figure: 0 h, not shown in the figure: 24, 72, 120 h) with healthy volunteers (HV). P -values are indicated above the respective data points. Asterisks indicate significant differences between HV and patients at 0 h only. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. AUC. Area under the curve; AU. Arbitrary units; PBS. Phosphate-buffered saline; E. coli Escherichia coli .

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Methodological workflow from sample preparation to measuring the cellular response capacity (CRC). Following blood collection, samples are prepared for flow cytometric analysis either unstimulated (PBS as buffer control) or stimulated with an inflammatory cocktail (cocktail of N-formylmethionyl-leucyl-phenylalanine, platelet-activating factor, and tumor necrosis factor). The CRC is calculated as the ratio of median fluorescence intensity (MFI) between stimulated and unstimulated neutrophils. Three approaches, classic, simple, and kinetic CRC, offer distinct advantages and limitations based on technical aspects such as manual processing steps (e.g., centrifugation) and incubation time. n =13–14. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing patients with sepsis at all time points (shown in the figure: 0 h, not shown in the figure: 24, 72, 120 h) with healthy volunteers (HV). P -values are indicated above the respective data points. Asterisks indicate significant differences between HV and patients at 0 h only. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. AUC. Area under the curve; AU. Arbitrary units; PBS. Phosphate-buffered saline; E. coli Escherichia coli .

    Article Snippet: Subsequently, the monovettes were exposed to either phosphate-buffered saline (PBS) with calcium and magnesium (PBS +/+ , #14080055, Gibco, Thermo Fisher Scientific, Waltham, USA) and the bacterial culture media [buffer control of Escherichia coli ( E. coli ) suspension, hereafter referred to as BuC], viable E. coli bacteria (ATCC line 25922, DSMZ, Braunschweig, Germany), or LPS (100 ng/ml, from E. coli O55:B5, #L2637, Sigma Aldrich, Steinheim, Germany).

    Techniques: Sample Prep, Control, Fluorescence, Centrifugation, Incubation, Saline

    Comparison of different approaches to measure the cellular response capacity (CRC) for CD11b on neutrophil granulocytes. Blood from healthy volunteers was incubated with either PBS (Control) or 100 ng/ml LPS for 60 min in the ex vivo whole blood model. a Analysis of the median fluorescence intensity (MFI). b Evaluation of the CRC as determined by different approaches (classic, simple, and kinetic CRC). c, d Change in fluorescence intensity and change in CRC using the kinetic CRC approach, comparing blood with previous exposure to LPS or PBS (buffer control) from the ex vivo whole blood model. In both c and d , the single-cell values measured by flow cytometry were condensed using a moving median with a window of 9 cells. This moving median was then approximated with a 5 th -degree polynomial function. The plots display these polynomial functions together with the baseline (median before stimulation) and a connecting line from the baseline to the polynomial function for 30 s after stimulation. Values are shown as median and interquartile range. n= 10. Statistical analysis was performed using the Mann-Whitney U test. ⁎⁎⁎ P <0.001. AU. Arbitrary units; LPS. Lipopolysaccharide; PBS. Phosphate-buffered saline.

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Comparison of different approaches to measure the cellular response capacity (CRC) for CD11b on neutrophil granulocytes. Blood from healthy volunteers was incubated with either PBS (Control) or 100 ng/ml LPS for 60 min in the ex vivo whole blood model. a Analysis of the median fluorescence intensity (MFI). b Evaluation of the CRC as determined by different approaches (classic, simple, and kinetic CRC). c, d Change in fluorescence intensity and change in CRC using the kinetic CRC approach, comparing blood with previous exposure to LPS or PBS (buffer control) from the ex vivo whole blood model. In both c and d , the single-cell values measured by flow cytometry were condensed using a moving median with a window of 9 cells. This moving median was then approximated with a 5 th -degree polynomial function. The plots display these polynomial functions together with the baseline (median before stimulation) and a connecting line from the baseline to the polynomial function for 30 s after stimulation. Values are shown as median and interquartile range. n= 10. Statistical analysis was performed using the Mann-Whitney U test. ⁎⁎⁎ P <0.001. AU. Arbitrary units; LPS. Lipopolysaccharide; PBS. Phosphate-buffered saline.

    Article Snippet: Subsequently, the monovettes were exposed to either phosphate-buffered saline (PBS) with calcium and magnesium (PBS +/+ , #14080055, Gibco, Thermo Fisher Scientific, Waltham, USA) and the bacterial culture media [buffer control of Escherichia coli ( E. coli ) suspension, hereafter referred to as BuC], viable E. coli bacteria (ATCC line 25922, DSMZ, Braunschweig, Germany), or LPS (100 ng/ml, from E. coli O55:B5, #L2637, Sigma Aldrich, Steinheim, Germany).

    Techniques: Comparison, Incubation, Control, Ex Vivo, Fluorescence, Single Cell, Flow Cytometry, MANN-WHITNEY, Saline

    Characterization of extracellular vesicles (EVs) derived from conditioned medium (CM). (a) Particle size distribution and concentration of EVs measured by nanoparticle tracking analysis. Median particle diameter: 143.6 nm; particle concentration: 1.6 × 10 9 particles/mL per 1 mL of unpurified CM prior to EV isolation. (b) Zeta potential of EVs measured in phosphate-buffered saline, showing a negatively charged surface of −36.38 mV. (c) Quantification of CD63-positive EVs using a Tim4–CD63 sandwich enzyme-linked immunosorbent assay. EV concentration is expressed per 1 mL of pre-purified CM. Statistical analysis using Welch's t -test demonstrated a significant difference between CM and EV samples ( n = 3 per group, ∗ p < 0.001).

    Journal: Regenerative Therapy

    Article Title: Dental pulp cell–derived conditioned culture media promotes periodontal repair through immunomodulation and bone regeneration

    doi: 10.1016/j.reth.2026.101144

    Figure Lengend Snippet: Characterization of extracellular vesicles (EVs) derived from conditioned medium (CM). (a) Particle size distribution and concentration of EVs measured by nanoparticle tracking analysis. Median particle diameter: 143.6 nm; particle concentration: 1.6 × 10 9 particles/mL per 1 mL of unpurified CM prior to EV isolation. (b) Zeta potential of EVs measured in phosphate-buffered saline, showing a negatively charged surface of −36.38 mV. (c) Quantification of CD63-positive EVs using a Tim4–CD63 sandwich enzyme-linked immunosorbent assay. EV concentration is expressed per 1 mL of pre-purified CM. Statistical analysis using Welch's t -test demonstrated a significant difference between CM and EV samples ( n = 3 per group, ∗ p < 0.001).

    Article Snippet: For subsequent passaging, the medium was removed and the dishes were rinsed twice with phosphate-buffered saline (Nacalai Tesque).

    Techniques: Derivative Assay, Concentration Assay, Isolation, Zeta Potential Analyzer, Saline, Sandwich ELISA, Purification