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phkcs  (Lonza)


Bioz Verified Symbol Lonza is a verified supplier
Bioz Manufacturer Symbol Lonza manufactures this product  
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    Lonza phkcs
    Phkcs, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phkcs/10__1096_slash_fj__202200079r-26-0-1?v=Lonza
    Average 90 stars, based on 1 article reviews
    phkcs - by Bioz Stars, 2026-07
    90/100 stars

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    TLR2 is linked to AD disease severity; sensory TLR2 is implicated in itch‐related gene transcription and OSM itch. (A) TLR2 transcription in human LAD and NLAD versus HC skin. FSL‐1 and Pam3CSK4 induce itch‐related transcripts in mTGNs (B). (C) Pam3CSK4 induces OSM transcription, but not OSMR. (D) OSMR and OSM transcription in human LAD, NLAD versus HC skin. Scratching bouts (E) after intradermal injection of OSM versus vehicle in mouse cheek models and time course (F) ( n = 9 mice/group). OSM induces AD‐related cytokine release (G), and dose‐dependently triggered calcium influx (H) <t>in</t> <t>cultured</t> <t>phKCs.</t> F∆, the change of stimulated fluorescence; (F∆/F0) max, maximum intensity of fluorescence over basal; Time max , time at which maximum fluorescence was achieved. Only cells showing a 20% increase over baseline were deemed as the responders. For (A–E), ***FDR < 0.001, **0.001 < FDR < 0.01, *0.01 < FDR < 0.05, ns FDR > 0.05. For (E–G), means ± SEMs ( n ≥ 3); N.S., * p > 0.05, and *** p < .001, student t ‐test.
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    TLR2 is linked to AD disease severity; sensory TLR2 is implicated in itch‐related gene transcription and OSM itch. (A) TLR2 transcription in human LAD and NLAD versus HC skin. FSL‐1 and Pam3CSK4 induce itch‐related transcripts in mTGNs (B). (C) Pam3CSK4 induces OSM transcription, but not OSMR. (D) OSMR and OSM transcription in human LAD, NLAD versus HC skin. Scratching bouts (E) after intradermal injection of OSM versus vehicle in mouse cheek models and time course (F) ( n = 9 mice/group). OSM induces AD‐related cytokine release (G), and dose‐dependently triggered calcium influx (H) in cultured phKCs. F∆, the change of stimulated fluorescence; (F∆/F0) max, maximum intensity of fluorescence over basal; Time max , time at which maximum fluorescence was achieved. Only cells showing a 20% increase over baseline were deemed as the responders. For (A–E), ***FDR < 0.001, **0.001 < FDR < 0.01, *0.01 < FDR < 0.05, ns FDR > 0.05. For (E–G), means ± SEMs ( n ≥ 3); N.S., * p > 0.05, and *** p < .001, student t ‐test.

    Journal: The FASEB Journal

    Article Title: The PLAUR signaling promotes chronic pruritus

    doi: 10.1096/fj.202200079R

    Figure Lengend Snippet: TLR2 is linked to AD disease severity; sensory TLR2 is implicated in itch‐related gene transcription and OSM itch. (A) TLR2 transcription in human LAD and NLAD versus HC skin. FSL‐1 and Pam3CSK4 induce itch‐related transcripts in mTGNs (B). (C) Pam3CSK4 induces OSM transcription, but not OSMR. (D) OSMR and OSM transcription in human LAD, NLAD versus HC skin. Scratching bouts (E) after intradermal injection of OSM versus vehicle in mouse cheek models and time course (F) ( n = 9 mice/group). OSM induces AD‐related cytokine release (G), and dose‐dependently triggered calcium influx (H) in cultured phKCs. F∆, the change of stimulated fluorescence; (F∆/F0) max, maximum intensity of fluorescence over basal; Time max , time at which maximum fluorescence was achieved. Only cells showing a 20% increase over baseline were deemed as the responders. For (A–E), ***FDR < 0.001, **0.001 < FDR < 0.01, *0.01 < FDR < 0.05, ns FDR > 0.05. For (E–G), means ± SEMs ( n ≥ 3); N.S., * p > 0.05, and *** p < .001, student t ‐test.

    Article Snippet: phKCs (Lonza) were cultured in KBM‐Gold medium supplemented with KBM‐Gold SingleQuot KC (Lonza) for 3 days before use.

    Techniques: Injection, Cell Culture, Fluorescence