pher4 (WuXi AppTec)
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90
Structured Review
WuXi AppTec
pher4
Pher4, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pher4/pher4+antibody/pmc02664939-120-73-74
Average 90 stars, based on 1 article reviews
Pher4, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pher4/pher4+antibody/pmc02664939-120-73-74
Average 90 stars, based on 1 article reviews
pher4 - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "Choice of Fixative Is Crucial to Successful Immunohistochemical Detection of Phosphoproteins in Paraffin-embedded Tumor Tissues"
Article Title: Choice of Fixative Is Crucial to Successful Immunohistochemical Detection of Phosphoproteins in Paraffin-embedded Tumor Tissues
Journal: Journal of Histochemistry and Cytochemistry
doi: 10.1369/jhc.2008.952911
Figure Legend Snippet: Antiphosphoprotein antibodies
Techniques Used: Concentration Assay
Figure Legend Snippet: IHC staining of phosphoproteins in xenograft tumor tissues
Techniques Used: Immunohistochemistry
Figure Legend Snippet: IHC staining of phosphoproteins in xenograft and human clinical tumor tissues. (A) SKOV-3 xenograft tumor tissues fixed in Streck’s tissue fixative (STF), 10% formalin, and 4% paraformaldehyde (PFA) were stained with anti-pMet antibody. (B) Human lung tumor tissue fixed in 10% formalin and STF were stained with anti-pHER4 antibody. Bar = 50 μm.
Techniques Used: Immunohistochemistry, Staining
Figure Legend Snippet: IHC staining of phosphoproteins in human clinical tumor tissues
Techniques Used: Immunohistochemistry
Related Articles
Concentration Assay:Article Title: Choice of Fixative Is Crucial to Successful Immunohistochemical Detection of Phosphoproteins in Paraffin-embedded Tumor Tissues Article Snippet: Sections were washed with TBS and incubated with labeled polymer-horseradish peroxidase (HRP) anti-rabbit or anti-mouse (DakoCytomation) for 30 min at room temperature and washed with TBS, and diaminobenzidine was used for development of a brown reaction product (DakoCytomation). .. The slides were washed with TBS and immersed in hematoxylin for 30 sec for counterstaining (Sigma; St. Louis, MO). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Vendor Phospho site Host species Concentration used pMET R&D (Minneapolis, MN) Y1234/Y1235 Rabbit 0.25 pg/ml pIGF-1R/IR Cell Signaling (Danvers, MA) Y1131/Y1 146 Rabbit 0.15 pg/ml pEGFR Cell Signaling Y992 Rabbit 1.0 pg/ml pHER2 Cell Signaling Y1221/Y1222 Rabbit 0.15 pg/ml pHER3 Cell Signaling Y1289 Rabbit 1.92 pg/ml Staining:Article Title: Choice of Fixative Is Crucial to Successful Immunohistochemical Detection of Phosphoproteins in Paraffin-embedded Tumor Tissues Article Snippet: Sections were washed with TBS and incubated with labeled polymer-horseradish peroxidase (HRP) anti-rabbit or anti-mouse (DakoCytomation) for 30 min at room temperature and washed with TBS, and diaminobenzidine was used for development of a brown reaction product (DakoCytomation). .. The slides were washed with TBS and immersed in hematoxylin for 30 sec for counterstaining (Sigma; St. Louis, MO). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Vendor Phospho site Host species Concentration used pMET R&D (Minneapolis, MN) Y1234/Y1235 Rabbit 0.25 pg/ml pIGF-1R/IR Cell Signaling (Danvers, MA) Y1131/Y1 146 Rabbit 0.15 pg/ml pEGFR Cell Signaling Y992 Rabbit 1.0 pg/ml pHER2 Cell Signaling Y1221/Y1222 Rabbit 0.15 pg/ml pHER3 Cell Signaling Y1289 Rabbit 1.92 pg/ml Incubation:Article Title: Choice of Fixative Is Crucial to Successful Immunohistochemical Detection of Phosphoproteins in Paraffin-embedded Tumor Tissues Article Snippet: Sections were washed with TBS and incubated with labeled polymer-horseradish peroxidase (HRP) anti-rabbit or anti-mouse (DakoCytomation) for 30 min at room temperature and washed with TBS, and diaminobenzidine was used for development of a brown reaction product (DakoCytomation). .. The slides were washed with TBS and immersed in hematoxylin for 30 sec for counterstaining (Sigma; St. Louis, MO). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Vendor Phospho site Host species Concentration used pMET R&D (Minneapolis, MN) Y1234/Y1235 Rabbit 0.25 pg/ml pIGF-1R/IR Cell Signaling (Danvers, MA) Y1131/Y1 146 Rabbit 0.15 pg/ml pEGFR Cell Signaling Y992 Rabbit 1.0 pg/ml pHER2 Cell Signaling Y1221/Y1222 Rabbit 0.15 pg/ml pHER3 Cell Signaling Y1289 Rabbit 1.92 pg/ml |