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diamidino 2 phenylindole  (Beyotime)


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    Structured Review

    Beyotime diamidino 2 phenylindole
    Diamidino 2 Phenylindole, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 33153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phenylindole/DAPI/pm42927029-233-5-7
    Average 99 stars, based on 33153 article reviews
    diamidino 2 phenylindole - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Circ_0000756 Mediates Oxidative Stress and Metabolic Memory in Diabetic Neuropathy via <scp>miR</scp> ‐7b Suppression of <scp>NRF2</scp> / <scp>ARE</scp> Pathway
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 5 of 19The FASEB Journal, 2025 488- conjugated secondary antibody (1:500, R37118, Invitrogen, USA) for 1 h at room temperature. .. Nuclei were counterstained with 4′,6- diamidino- 2- phenylindole (DAPI, C1002, Beyotime, China), and nuclear localization was visualized by fluorescence microscopy (Leica DMi8, Germany). ..

    Article Title: Molecular Mechanism of the IRF1/NFE2L1-DT/ALKBH5/Cx43 Axis in Radiation-Induced Injury in Vascular Endothelial Cells Through Pyroptosis.
    Article Snippet: The cells were then incubated with an IgG secondary antibody (ab150079, 1:1000, Abcam) for 1 h at 37°C. .. Nuclear staining was performed using 4′,6- diamidino- 2- phenylindole (DAPI; C1002, Beyotime), and fluorescence imaging was captured using an FV3000 confocal laser scanning microscope (Olympus, Tokyo, Japan). .. Quantitative analysis of γ- H2AX foci was performed with ImageJ software (NIH, Bethesda, MD, USA).

    Article Title: NSUN2-FOSB reciprocity facilitates leukemogenesis in an m5C-dependent manner by increasing BCL2L1 expression.
    Article Snippet: Apoptosis was measured using Annexin V/7-AAD staining (BD PharMingen, San Diego, CA, USA). .. Cellular DNA was stained with 4,6-diamidino-2- phenylindole (DAPI, Beyotime Biotechnology), and fluorescence signals were detected by a laser scanning confocal microscope (Leica Stellaris 5, Leica Microsystems Inc., Deerfield, IL, USA). mRNA half-life (t1/2) measurement AML cells were transduced with sh-NSUN2 or sh-NC. .. Cells were then resuspended in 400 L of 1×binding buffer, and apoptotic cells were analyzed by flow cytometry (CytoFLEX LX, Beckman-Coulter, Brea, CA, USA) within 30 min after staining.

    Article Title: Epigallocatechin-3-Gallate (EGCG) Mitigates Deep Vein Thrombosis Through Antioxidant and Anti-Ferroptotic Actions via the NRF2-NEDD4 Pathway.
    Article Snippet: Deep vein thrombosis (DVT) is a prevalent vascular disorder associated with high morbidity and mortality.. Evidence suggests that ferroptosis plays a critical role in thrombus formation.. Epigallocatechin3gallate (EGCG), the primary bioactive component of green tea, possesses antioxidant and antiinflammatory properties, but its role and molecular mechanism in regulating ferroptosis in DVT remain unclear.

    Microscopy:

    Article Title: Circ_0000756 Mediates Oxidative Stress and Metabolic Memory in Diabetic Neuropathy via <scp>miR</scp> ‐7b Suppression of <scp>NRF2</scp> / <scp>ARE</scp> Pathway
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 5 of 19The FASEB Journal, 2025 488- conjugated secondary antibody (1:500, R37118, Invitrogen, USA) for 1 h at room temperature. .. Nuclei were counterstained with 4′,6- diamidino- 2- phenylindole (DAPI, C1002, Beyotime, China), and nuclear localization was visualized by fluorescence microscopy (Leica DMi8, Germany). ..

    Article Title: NSUN2-FOSB reciprocity facilitates leukemogenesis in an m5C-dependent manner by increasing BCL2L1 expression.
    Article Snippet: Apoptosis was measured using Annexin V/7-AAD staining (BD PharMingen, San Diego, CA, USA). .. Cellular DNA was stained with 4,6-diamidino-2- phenylindole (DAPI, Beyotime Biotechnology), and fluorescence signals were detected by a laser scanning confocal microscope (Leica Stellaris 5, Leica Microsystems Inc., Deerfield, IL, USA). mRNA half-life (t1/2) measurement AML cells were transduced with sh-NSUN2 or sh-NC. .. Cells were then resuspended in 400 L of 1×binding buffer, and apoptotic cells were analyzed by flow cytometry (CytoFLEX LX, Beckman-Coulter, Brea, CA, USA) within 30 min after staining.

    Article Title: Epigallocatechin-3-Gallate (EGCG) Mitigates Deep Vein Thrombosis Through Antioxidant and Anti-Ferroptotic Actions via the NRF2-NEDD4 Pathway.
    Article Snippet: Deep vein thrombosis (DVT) is a prevalent vascular disorder associated with high morbidity and mortality.. Evidence suggests that ferroptosis plays a critical role in thrombus formation.. Epigallocatechin3gallate (EGCG), the primary bioactive component of green tea, possesses antioxidant and antiinflammatory properties, but its role and molecular mechanism in regulating ferroptosis in DVT remain unclear.

    Staining:

    Article Title: Male-biased Cyp17a2 orchestrates antiviral sexual dimorphism in fish via STING stabilization and viral protein degradation
    Article Snippet: After being washed three times by PBS, the samples were incubated with secondary Ab (Goat anti- Rabbit IgG (H+L) Highly Cross- Adsorbed Secondary Antibody, Alexa Fluor Plus 488) (Thermo Scientific, A32731, 1:5000) in PBS containing 2% BSA for 1 hr at room temperature. .. After additional PBS washing, the cells were finally stained with 1 μg/ml 4′, 6- diamidino- 2- phenylindole (DAPI, Beyotime Institute of Biotechnology, C1006) for 10 min in the dark at room temperature. .. Finally, the coverslips were washed and observed with a confocal microscope under a 10×immersion objective (SP8; Leica).

    Article Title: <scp>HDAC</scp> Inhibitor <scp>SAHA</scp> Attenuates Inflammatory Injury in Subarachnoid Hemorrhage by Suppressing <scp>NLRP1</scp> Signaling‐Mediated Neuronal Ferroptosis via Enhanced <scp>KLF2</scp> Acetylation
    Article Snippet: Subarachnoid hemorrhage (SAH) is an emergency with high mortality and disability rates.. Targeting ferroptosis shows potential for ameliorating neuroinflammatory injury after SAH.. A rat model of SAH was established via the endovascular puncture method.

    Article Title: Mechanism of Tumour-Associated Macrophage-Derived Exosomes in Chemo-Sensitivity of Laryngeal Cancer by Regulating Aerobic Glycolysis via WTAP/GLUT-1.
    Article Snippet: Laryngeal cancer (LC) is a condition characterised by cancerous cell development in laryngeal tissues.. This article focuses on the mechanism of tumourassociated macrophagederived exosomes (TAMExos) in LC chemosensitivity by mediating Wilms' tumour 1associating protein (WTAP)/glucose transporter1 (GLUT1).. Human peripheral blood mononuclear cells (PBMCs) were cultured in vitro.

    Article Title: Molecular Mechanism of the IRF1/NFE2L1-DT/ALKBH5/Cx43 Axis in Radiation-Induced Injury in Vascular Endothelial Cells Through Pyroptosis.
    Article Snippet: The cells were then incubated with an IgG secondary antibody (ab150079, 1:1000, Abcam) for 1 h at 37°C. .. Nuclear staining was performed using 4′,6- diamidino- 2- phenylindole (DAPI; C1002, Beyotime), and fluorescence imaging was captured using an FV3000 confocal laser scanning microscope (Olympus, Tokyo, Japan). .. Quantitative analysis of γ- H2AX foci was performed with ImageJ software (NIH, Bethesda, MD, USA).

    Article Title: NSUN2-FOSB reciprocity facilitates leukemogenesis in an m5C-dependent manner by increasing BCL2L1 expression.
    Article Snippet: Apoptosis was measured using Annexin V/7-AAD staining (BD PharMingen, San Diego, CA, USA). .. Cellular DNA was stained with 4,6-diamidino-2- phenylindole (DAPI, Beyotime Biotechnology), and fluorescence signals were detected by a laser scanning confocal microscope (Leica Stellaris 5, Leica Microsystems Inc., Deerfield, IL, USA). mRNA half-life (t1/2) measurement AML cells were transduced with sh-NSUN2 or sh-NC. .. Cells were then resuspended in 400 L of 1×binding buffer, and apoptotic cells were analyzed by flow cytometry (CytoFLEX LX, Beckman-Coulter, Brea, CA, USA) within 30 min after staining.

    Incubation:

    Article Title: <scp>HDAC</scp> Inhibitor <scp>SAHA</scp> Attenuates Inflammatory Injury in Subarachnoid Hemorrhage by Suppressing <scp>NLRP1</scp> Signaling‐Mediated Neuronal Ferroptosis via Enhanced <scp>KLF2</scp> Acetylation
    Article Snippet: Subarachnoid hemorrhage (SAH) is an emergency with high mortality and disability rates.. Targeting ferroptosis shows potential for ameliorating neuroinflammatory injury after SAH.. A rat model of SAH was established via the endovascular puncture method.

    Imaging:

    Article Title: Molecular Mechanism of the IRF1/NFE2L1-DT/ALKBH5/Cx43 Axis in Radiation-Induced Injury in Vascular Endothelial Cells Through Pyroptosis.
    Article Snippet: The cells were then incubated with an IgG secondary antibody (ab150079, 1:1000, Abcam) for 1 h at 37°C. .. Nuclear staining was performed using 4′,6- diamidino- 2- phenylindole (DAPI; C1002, Beyotime), and fluorescence imaging was captured using an FV3000 confocal laser scanning microscope (Olympus, Tokyo, Japan). .. Quantitative analysis of γ- H2AX foci was performed with ImageJ software (NIH, Bethesda, MD, USA).

    Laser-Scanning Microscopy:

    Article Title: Molecular Mechanism of the IRF1/NFE2L1-DT/ALKBH5/Cx43 Axis in Radiation-Induced Injury in Vascular Endothelial Cells Through Pyroptosis.
    Article Snippet: The cells were then incubated with an IgG secondary antibody (ab150079, 1:1000, Abcam) for 1 h at 37°C. .. Nuclear staining was performed using 4′,6- diamidino- 2- phenylindole (DAPI; C1002, Beyotime), and fluorescence imaging was captured using an FV3000 confocal laser scanning microscope (Olympus, Tokyo, Japan). .. Quantitative analysis of γ- H2AX foci was performed with ImageJ software (NIH, Bethesda, MD, USA).

    Quantitative RT-PCR:

    Article Title: Aberrant <scp>DNMTs</scp> Promote <scp>TXNIP</scp> Upregulation and Ovarian Fibrosis in <scp>PCOS</scp>
    Article Snippet: After washing with PBST three times, cells were incubated at 25°C for 2 h with fluorescent secondary antibodies (Beyotime). .. Nuclei were counterstained with 4′,6- diamidino- 2- phenylindole (DAPI; Beyotime) at a TABLE 1 | qRT- PCR primers used in the study. ..

    Transduction:

    Article Title: NSUN2-FOSB reciprocity facilitates leukemogenesis in an m5C-dependent manner by increasing BCL2L1 expression.
    Article Snippet: Apoptosis was measured using Annexin V/7-AAD staining (BD PharMingen, San Diego, CA, USA). .. Cellular DNA was stained with 4,6-diamidino-2- phenylindole (DAPI, Beyotime Biotechnology), and fluorescence signals were detected by a laser scanning confocal microscope (Leica Stellaris 5, Leica Microsystems Inc., Deerfield, IL, USA). mRNA half-life (t1/2) measurement AML cells were transduced with sh-NSUN2 or sh-NC. .. Cells were then resuspended in 400 L of 1×binding buffer, and apoptotic cells were analyzed by flow cytometry (CytoFLEX LX, Beckman-Coulter, Brea, CA, USA) within 30 min after staining.



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    Changes in myocardial macrophage phenotypes and inflammatory cytokine profiles after myocardial infarction (MI). (A) Representative immunofluorescence images of heart sections from the Control, Gel@PPM, Gel@PP@Rapa, Gel@OVA-IVNMs, and Gel@IVNMs groups at 7 and 56 d after MI. Nuclei were <t>stained</t> with <t>4′,6-diamidino-2-phenylindole</t> (DAPI; blue), macrophages with CD68 (green), proinflammatory macrophage-associated inducible nitric oxide synthase (iNOS) with red fluorescence, and reparative/immunomodulatory macrophage-associated CD206 with purple fluorescence. Scale bars, 200 μm. (B) Quantification of iNOS- and CD206-positive signals in myocardial sections at 7 and 56 d after MI. (C) Enzyme-linked immunosorbent assay quantification of interleukin-2 (IL-2), interferon-γ (IFN-γ), transforming growth factor-β (TGF-β), and IL-10 levels in myocardial tissue at 7 and 56 d after MI. Data are presented as means ± SD, with individual points representing biological replicates. Statistical significance is indicated as ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.
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    Image Search Results


    Changes in myocardial macrophage phenotypes and inflammatory cytokine profiles after myocardial infarction (MI). (A) Representative immunofluorescence images of heart sections from the Control, Gel@PPM, Gel@PP@Rapa, Gel@OVA-IVNMs, and Gel@IVNMs groups at 7 and 56 d after MI. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue), macrophages with CD68 (green), proinflammatory macrophage-associated inducible nitric oxide synthase (iNOS) with red fluorescence, and reparative/immunomodulatory macrophage-associated CD206 with purple fluorescence. Scale bars, 200 μm. (B) Quantification of iNOS- and CD206-positive signals in myocardial sections at 7 and 56 d after MI. (C) Enzyme-linked immunosorbent assay quantification of interleukin-2 (IL-2), interferon-γ (IFN-γ), transforming growth factor-β (TGF-β), and IL-10 levels in myocardial tissue at 7 and 56 d after MI. Data are presented as means ± SD, with individual points representing biological replicates. Statistical significance is indicated as ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

    Journal: Research

    Article Title: Inverse Vaccine Nanomimics: In situ Antigen-Specific Immune Tolerance Induction for Durable Cardiac Repair Post-Myocardial Infarction

    doi: 10.34133/research.1464

    Figure Lengend Snippet: Changes in myocardial macrophage phenotypes and inflammatory cytokine profiles after myocardial infarction (MI). (A) Representative immunofluorescence images of heart sections from the Control, Gel@PPM, Gel@PP@Rapa, Gel@OVA-IVNMs, and Gel@IVNMs groups at 7 and 56 d after MI. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue), macrophages with CD68 (green), proinflammatory macrophage-associated inducible nitric oxide synthase (iNOS) with red fluorescence, and reparative/immunomodulatory macrophage-associated CD206 with purple fluorescence. Scale bars, 200 μm. (B) Quantification of iNOS- and CD206-positive signals in myocardial sections at 7 and 56 d after MI. (C) Enzyme-linked immunosorbent assay quantification of interleukin-2 (IL-2), interferon-γ (IFN-γ), transforming growth factor-β (TGF-β), and IL-10 levels in myocardial tissue at 7 and 56 d after MI. Data are presented as means ± SD, with individual points representing biological replicates. Statistical significance is indicated as ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

    Article Snippet: For T cell immunofluorescence labeling, nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (E-CK-A163, Elabscience, China), CD4 with FITC (E-AB-F1097C, Elabscience, China), and CD127 with Alexa Fluor 555 (16-1271-85, eBioscience, USA).

    Techniques: Immunofluorescence, Control, Staining, Fluorescence, Enzyme-linked Immunosorbent Assay, Tissue