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4 6 diamidino 2 phenylindole  (Beyotime)


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    Structured Review

    Beyotime 4 6 diamidino 2 phenylindole
    4 6 Diamidino 2 Phenylindole, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32073 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phenylindole/DAPI/pmc12999324-97-30-32
    Average 99 stars, based on 32073 article reviews
    4 6 diamidino 2 phenylindole - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Circ_0000756 Mediates Oxidative Stress and Metabolic Memory in Diabetic Neuropathy via <scp>miR</scp> ‐7b Suppression of <scp>NRF2</scp> / <scp>ARE</scp> Pathway
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 5 of 19The FASEB Journal, 2025 488- conjugated secondary antibody (1:500, R37118, Invitrogen, USA) for 1 h at room temperature. .. Nuclei were counterstained with 4′,6- diamidino- 2- phenylindole (DAPI, C1002, Beyotime, China), and nuclear localization was visualized by fluorescence microscopy (Leica DMi8, Germany). ..

    Article Title: Molecular Mechanism of the IRF1/NFE2L1-DT/ALKBH5/Cx43 Axis in Radiation-Induced Injury in Vascular Endothelial Cells Through Pyroptosis.
    Article Snippet: The cells were then incubated with an IgG secondary antibody (ab150079, 1:1000, Abcam) for 1 h at 37°C. .. Nuclear staining was performed using 4′,6- diamidino- 2- phenylindole (DAPI; C1002, Beyotime), and fluorescence imaging was captured using an FV3000 confocal laser scanning microscope (Olympus, Tokyo, Japan). .. Quantitative analysis of γ- H2AX foci was performed with ImageJ software (NIH, Bethesda, MD, USA).

    Article Title: NSUN2-FOSB reciprocity facilitates leukemogenesis in an m5C-dependent manner by increasing BCL2L1 expression.
    Article Snippet: .. Cellular DNA was stained with 4,6-diamidino-2- phenylindole (DAPI, Beyotime Biotechnology), and fluorescence signals were detected by a laser scanning confocal microscope (Leica Stellaris 5, Leica Microsystems Inc., Deerfield, IL, USA). mRNA half-life (t1/2) measurement AML cells were transduced with sh-NSUN2 or sh-NC. .. After 48 h of transduction, AML cells were treated with 2 M actinomycin D (ActD, TargetMol) at various time points.

    Article Title: Epigallocatechin-3-Gallate (EGCG) Mitigates Deep Vein Thrombosis Through Antioxidant and Anti-Ferroptotic Actions via the NRF2-NEDD4 Pathway.
    Article Snippet: Deep vein thrombosis (DVT) is a prevalent vascular disorder associated with high morbidity and mortality.. Evidence suggests that ferroptosis plays a critical role in thrombus formation.. Epigallocatechin3gallate (EGCG), the primary bioactive component of green tea, possesses antioxidant and antiinflammatory properties, but its role and molecular mechanism in regulating ferroptosis in DVT remain unclear.

    Microscopy:

    Article Title: Circ_0000756 Mediates Oxidative Stress and Metabolic Memory in Diabetic Neuropathy via <scp>miR</scp> ‐7b Suppression of <scp>NRF2</scp> / <scp>ARE</scp> Pathway
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 5 of 19The FASEB Journal, 2025 488- conjugated secondary antibody (1:500, R37118, Invitrogen, USA) for 1 h at room temperature. .. Nuclei were counterstained with 4′,6- diamidino- 2- phenylindole (DAPI, C1002, Beyotime, China), and nuclear localization was visualized by fluorescence microscopy (Leica DMi8, Germany). ..

    Article Title: NSUN2-FOSB reciprocity facilitates leukemogenesis in an m5C-dependent manner by increasing BCL2L1 expression.
    Article Snippet: .. Cellular DNA was stained with 4,6-diamidino-2- phenylindole (DAPI, Beyotime Biotechnology), and fluorescence signals were detected by a laser scanning confocal microscope (Leica Stellaris 5, Leica Microsystems Inc., Deerfield, IL, USA). mRNA half-life (t1/2) measurement AML cells were transduced with sh-NSUN2 or sh-NC. .. After 48 h of transduction, AML cells were treated with 2 M actinomycin D (ActD, TargetMol) at various time points.

    Article Title: Epigallocatechin-3-Gallate (EGCG) Mitigates Deep Vein Thrombosis Through Antioxidant and Anti-Ferroptotic Actions via the NRF2-NEDD4 Pathway.
    Article Snippet: Deep vein thrombosis (DVT) is a prevalent vascular disorder associated with high morbidity and mortality.. Evidence suggests that ferroptosis plays a critical role in thrombus formation.. Epigallocatechin3gallate (EGCG), the primary bioactive component of green tea, possesses antioxidant and antiinflammatory properties, but its role and molecular mechanism in regulating ferroptosis in DVT remain unclear.

    Staining:

    Article Title: Male-biased Cyp17a2 orchestrates antiviral sexual dimorphism in fish via STING stabilization and viral protein degradation
    Article Snippet: After being washed three times by PBS, the samples were incubated with secondary Ab (Goat anti- Rabbit IgG (H+L) Highly Cross- Adsorbed Secondary Antibody, Alexa Fluor Plus 488) (Thermo Scientific, A32731, 1:5000) in PBS containing 2% BSA for 1 hr at room temperature. .. After additional PBS washing, the cells were finally stained with 1 μg/ml 4′, 6- diamidino- 2- phenylindole (DAPI, Beyotime Institute of Biotechnology, C1006) for 10 min in the dark at room temperature. .. Finally, the coverslips were washed and observed with a confocal microscope under a 10×immersion objective (SP8; Leica).

    Article Title: Mechanism of Tumour-Associated Macrophage-Derived Exosomes in Chemo-Sensitivity of Laryngeal Cancer by Regulating Aerobic Glycolysis via WTAP/GLUT-1.
    Article Snippet: Laryngeal cancer (LC) is a condition characterised by cancerous cell development in laryngeal tissues.. This article focuses on the mechanism of tumourassociated macrophagederived exosomes (TAMExos) in LC chemosensitivity by mediating Wilms' tumour 1associating protein (WTAP)/glucose transporter1 (GLUT1).. Human peripheral blood mononuclear cells (PBMCs) were cultured in vitro.

    Article Title: Molecular Mechanism of the IRF1/NFE2L1-DT/ALKBH5/Cx43 Axis in Radiation-Induced Injury in Vascular Endothelial Cells Through Pyroptosis.
    Article Snippet: The cells were then incubated with an IgG secondary antibody (ab150079, 1:1000, Abcam) for 1 h at 37°C. .. Nuclear staining was performed using 4′,6- diamidino- 2- phenylindole (DAPI; C1002, Beyotime), and fluorescence imaging was captured using an FV3000 confocal laser scanning microscope (Olympus, Tokyo, Japan). .. Quantitative analysis of γ- H2AX foci was performed with ImageJ software (NIH, Bethesda, MD, USA).

    Article Title: NSUN2-FOSB reciprocity facilitates leukemogenesis in an m5C-dependent manner by increasing BCL2L1 expression.
    Article Snippet: .. Cellular DNA was stained with 4,6-diamidino-2- phenylindole (DAPI, Beyotime Biotechnology), and fluorescence signals were detected by a laser scanning confocal microscope (Leica Stellaris 5, Leica Microsystems Inc., Deerfield, IL, USA). mRNA half-life (t1/2) measurement AML cells were transduced with sh-NSUN2 or sh-NC. .. After 48 h of transduction, AML cells were treated with 2 M actinomycin D (ActD, TargetMol) at various time points.

    Incubation:

    Article Title: The fungicide jinggangmycin stimulates fecundity of Nilaparvata lugens Stål via ILP/Foxo signaling.
    Article Snippet: The brown planthopper (BPH), Nilaparvata lugens is a typical pesticide-induced resurgent rice pest.. A previous study showed that a fungicide, jinggangmycin (JGM)-treated rice led to markedly increased sugar content and (Insulin-like Peptide 2) ILP2 in response to sugar-mediated TOR signaling and stimulated fecundity in BPH.. However, the role of the other ILPs in response to types of carbohydrate compounds remained poorly understood.

    Imaging:

    Article Title: Molecular Mechanism of the IRF1/NFE2L1-DT/ALKBH5/Cx43 Axis in Radiation-Induced Injury in Vascular Endothelial Cells Through Pyroptosis.
    Article Snippet: The cells were then incubated with an IgG secondary antibody (ab150079, 1:1000, Abcam) for 1 h at 37°C. .. Nuclear staining was performed using 4′,6- diamidino- 2- phenylindole (DAPI; C1002, Beyotime), and fluorescence imaging was captured using an FV3000 confocal laser scanning microscope (Olympus, Tokyo, Japan). .. Quantitative analysis of γ- H2AX foci was performed with ImageJ software (NIH, Bethesda, MD, USA).

    Laser-Scanning Microscopy:

    Article Title: Molecular Mechanism of the IRF1/NFE2L1-DT/ALKBH5/Cx43 Axis in Radiation-Induced Injury in Vascular Endothelial Cells Through Pyroptosis.
    Article Snippet: The cells were then incubated with an IgG secondary antibody (ab150079, 1:1000, Abcam) for 1 h at 37°C. .. Nuclear staining was performed using 4′,6- diamidino- 2- phenylindole (DAPI; C1002, Beyotime), and fluorescence imaging was captured using an FV3000 confocal laser scanning microscope (Olympus, Tokyo, Japan). .. Quantitative analysis of γ- H2AX foci was performed with ImageJ software (NIH, Bethesda, MD, USA).

    Quantitative RT-PCR:

    Article Title: Aberrant <scp>DNMTs</scp> Promote <scp>TXNIP</scp> Upregulation and Ovarian Fibrosis in <scp>PCOS</scp>
    Article Snippet: After washing with PBST three times, cells were incubated at 25°C for 2 h with fluorescent secondary antibodies (Beyotime). .. Nuclei were counterstained with 4′,6- diamidino- 2- phenylindole (DAPI; Beyotime) at a TABLE 1 | qRT- PCR primers used in the study. ..

    Transduction:

    Article Title: NSUN2-FOSB reciprocity facilitates leukemogenesis in an m5C-dependent manner by increasing BCL2L1 expression.
    Article Snippet: .. Cellular DNA was stained with 4,6-diamidino-2- phenylindole (DAPI, Beyotime Biotechnology), and fluorescence signals were detected by a laser scanning confocal microscope (Leica Stellaris 5, Leica Microsystems Inc., Deerfield, IL, USA). mRNA half-life (t1/2) measurement AML cells were transduced with sh-NSUN2 or sh-NC. .. After 48 h of transduction, AML cells were treated with 2 M actinomycin D (ActD, TargetMol) at various time points.



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