pgreenlantern-1 (5,030 bp) coding for gfp under the control of cmv promoter (Thermo Fisher)
Thermo Fisher is a verified supplier 
Thermo Fisher manufactures this product 
90
Structured Review
Thermo Fisher
pgreenlantern-1 (5,030 bp) coding for gfp under the control of cmv promoter
Pgreenlantern 1 (5,030 Bp) Coding For Gfp Under The Control Of Cmv Promoter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgreenlantern-1/pmc05703459-53-20-32
Average 90 stars, based on 1 article reviews
Pgreenlantern 1 (5,030 Bp) Coding For Gfp Under The Control Of Cmv Promoter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgreenlantern-1/pmc05703459-53-20-32
Average 90 stars, based on 1 article reviews
pgreenlantern-1 (5,030 bp) coding for gfp under the control of cmv promoter - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion"
Article Title: Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion
Journal: PLoS ONE
doi: 10.1371/journal.pone.0188239
Figure Legend Snippet: A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 300 μm is common for slices at different levels and of 50 μm for details. B. TH(+) neuron counting. C. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.
Techniques Used: Immunohistochemistry, Transfection
Figure Legend Snippet: A. Representative micrographs of TH immunohistochemistry. Headings = Anterior-posterior coordinates of Paxinos and Watson Rat Atlas . 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale bar of 1 mm is for slices at different levels and of 100 μm for details. B. Densitometry of TH(+) relative area. 4WT and 12 WT = 4 and 12 weeks after transfection. 24WL = untransfected rats with 24 weeks of lesion. 12WL = 12 weeks after lesion. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. One-way ANOVA and Newman-Keuls post-test. * P < 0.001, ** P < 0.0001. ns = no statistical significance, P > 0.05.
Techniques Used: Immunohistochemistry, Transfection
Figure Legend Snippet: A. Representative micrographs of double immunofluorescence staining against NTSR1 and TH. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale is common for all micrographs. B. immunofluorescence area density (IFAD) for NTSR1 and TH was determined using ImageJ software v.1.46r (National Institutes of Health; Bethesda, MD). NRTN4WT and NRTN412WT = 4 and 12 weeks after transfection. UT = untransfected rats. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM ( n = 3 independent rats for each experimental condition). Two-way ANOVA and Bonferroni post-test. ns = no statistical significance.
Techniques Used: Double Immunofluorescence Staining, Transfection, Immunofluorescence, Software
Figure Legend Snippet: A. Representative micrographs of double immunofluorescence staining against NTSR1 and TH. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The scale is common for all micrographs. B. immunofluorescence area density (IFAD) for NTSR1 and TH was determined using ImageJ software v.1.46r (National Institutes of Health; Bethesda, MD). NRTN4WT and NRTN412WT = 4 and 12 weeks after transfection. UT = untransfected rats. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. All values are the mean ± SEM (n = 3 independent rats for each experimental condition). Two-way ANOVA and Bonferroni post-test. ns = no statistical significance.
Techniques Used: Double Immunofluorescence Staining, Transfection, Immunofluorescence, Software
Figure Legend Snippet: Representative micrographs of double immunofluorescence to TH and β-III-tubulin. 24WLUT = Untransfected rats with 24 weeks of 6-OHDA lesion. 12WL4WT = 12 weeks after lesion and 4 weeks after transfection, 12WL12WT = 12 weeks after lesion and 12 weeks after transfection. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. The scale bar is common for all micrographs.
Techniques Used: Immunofluorescence, Transfection
Figure Legend Snippet: A . Sholl diagram for the morphological study of dendrites and dendritic spines. B . Representative micrograph of a medium spiny neuron (MSN) of a healthy striatum. The arrow shows the 50 μm segment (primary dendrite) where dendritic spines were analyzed. Upper calibration bar = 25 μm. The small panels show different types of spines (thin, mushroom, stubby and wide, from left to right) indicated by the arrow. Calibration bar = 5 μm. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion and the Sholl analysis was performed at the end of the study (12 weeks after transfection or 24 weeks after lesion). UT = untransfected rats with lesion. C. Sholl analysis of total number of intersections along dendritic trees in MSNs at all distances in 200-μm radius from the soma. D. Plot of mean spine density analyzed per 50-μm primary dendrite from cell body (proximal segment, upper arrow in B ) from six neurons per rat. All values represent the mean ± SEM ( n = 6 independent rats in each experimental condition). Statistical analysis ( C and D ) was performed using one-way ANOVA and Tukey post-hoc test.
Techniques Used: Transfection
Figure Legend Snippet: Ipsilateral circling behavior ( A and C ) was activated by methamphetamine (8 mg/kg; i.p.), and contralateral circling behavior ( B and D ) was activated by apomorphine (0.5 mg/kg; i.p.) at weeks 0, 4 and 12 after transfection. The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion (Week 0). All values represent the mean ± SEM ( n = 4 independent rats in each experimental condition). One-way ANOVA and Newman-Keuls post-test. * P <0.0005, ** P < 0.0001.
Techniques Used: Transfection
Figure Legend Snippet: The transfections of pGreenLantern-1 (GFP) and pTracer-mNRTN-His (NRTN) plasmids were made at week 12 after lesion. UT = untransfected rats. The tests were performed at week 4 (12WL4WT) and week 12 (12WL12WT) after transfection. All values represent the mean ± SEM ( n = 4 independent rats in each experimental condition). * P < 0.001 when compared the values of the contralateral limb (Contra limb) with those of the ipsilateral limb (Ipsi limb), ** P < 0.001 when compared NRTN-His vs UT and § P < 0.001, NRTN-His vs GFP using two-way ANOVA. The post-hoc test was Newman-Keuls analysis.
Techniques Used: Transfection
Related Articles
Variant Assay:Article Title: Methods for screening ligands that activate the translocation of a steroid receptor to the nucleus in mammalian cells Article Snippet: For example, Article Title: Transcriptional termination modulated by nucleotides outside the characterized gene end sequence of respiratory syncytial virus. Article Snippet: M11486 and M75730): the RS viral leader (A2 nt 1 to 44); the first 48 nt of the NS1 gene (A2 nt 45 to 92), with a single nt change at A2 nt 90 that resulted in the creation of an AvrII site; nucleotides 10 to 410 of the SH gene (A2 nt 4229 to 4628); the 44 nt SH/G intergenic region (A2 nt 4629 to 4672); the 9 nt G GS sequence (A2 nt 4673 to 4682); an inserted KpnI site; a 734-nt sequence containing the GFP open reading frame from Article Title: Distinct effect of pregnenolone sulfate on NMDA receptor subtypes Article Snippet: Using rapid agonist applications to transfected HEK-293 cells, we investigated pregnenolone sulfate (PS) effects on deactivation and desensitization of recombinant NMDA receptors subtypes.. PS prolonged the deactivation of responses produced by brief applications of L-glutamate with all subunit combinations tested.. The action of PS was larger on NR1a/NR2A than on NR1a/NR2B channels. Article Title: Chimeric protein comprising a green fluorescent ptotein fused to a transcription factor Article Snippet: For example, Article Title: Human ClC-3 Is Not the Swelling-activated Chloride Channel Involved in Cell Volume Regulation Article Snippet: To generate a cell line expressing short hClC-3 without a GFP tag, HEK293 cells were cotransfected with pGreenLantern-1, which encodes GFP ( Article Title: Proteinase-Polymerase Precursor as the Active Form of Feline Calicivirus RNA-Dependent RNA Polymerase Article Snippet: Plasmid pGLT7 was constructed by subcloning the 732-bp NotI fragment of Article Title: High level Purkinje cell specific expression of green fluorescent protein in transgenic mice. Article Snippet: The green fluorescent protein (GFP) has become a powerful tool in molecular and cell biology.. It is a commonly used marker for cloning and transfection experiments as well as a useful label of living cells allowing continuous observation of developing structures.. In order to unravel mechanisms of neuronal differentiation, we generated a transgenic mouse model which expresses GFPS65T,hu under the control of the Purkinje cellspecific promoter L7/pcp-2. Article Title: Green fluorescent protein causes mitochondria to aggregate in the presence of the Bcl-2 family proteins. Article Snippet: Green fluorescent protein (GFP) has been widely used in a variety of experiments in cell biology.. When cells were co-transfected with the GFP gene and the bcl-2 family genes bcl-2, bcl-xL, and bax, mitochondria appeared to aggregate at the periphery of the nucleus specifically where GFP was expressed.. Little aggregation was seen in the presence of other members of the GFP family, EGFP (enhanced GFP), ECFP (enhanced cyan variant), and EYFP (enhanced yellow-green variant). Sequencing:Article Title: Methods for screening ligands that activate the translocation of a steroid receptor to the nucleus in mammalian cells Article Snippet: For example, Article Title: Transcriptional termination modulated by nucleotides outside the characterized gene end sequence of respiratory syncytial virus. Article Snippet: M11486 and M75730): the RS viral leader (A2 nt 1 to 44); the first 48 nt of the NS1 gene (A2 nt 45 to 92), with a single nt change at A2 nt 90 that resulted in the creation of an AvrII site; nucleotides 10 to 410 of the SH gene (A2 nt 4229 to 4628); the 44 nt SH/G intergenic region (A2 nt 4629 to 4672); the 9 nt G GS sequence (A2 nt 4673 to 4682); an inserted KpnI site; a 734-nt sequence containing the GFP open reading frame from Article Title: Distinct effect of pregnenolone sulfate on NMDA receptor subtypes Article Snippet: Using rapid agonist applications to transfected HEK-293 cells, we investigated pregnenolone sulfate (PS) effects on deactivation and desensitization of recombinant NMDA receptors subtypes.. PS prolonged the deactivation of responses produced by brief applications of L-glutamate with all subunit combinations tested.. The action of PS was larger on NR1a/NR2A than on NR1a/NR2B channels. Article Title: Chimeric protein comprising a green fluorescent ptotein fused to a transcription factor Article Snippet: For example, Article Title: Human ClC-3 Is Not the Swelling-activated Chloride Channel Involved in Cell Volume Regulation Article Snippet: To generate a cell line expressing short hClC-3 without a GFP tag, HEK293 cells were cotransfected with pGreenLantern-1, which encodes GFP ( Article Title: Proteinase-Polymerase Precursor as the Active Form of Feline Calicivirus RNA-Dependent RNA Polymerase Article Snippet: Plasmid pGLT7 was constructed by subcloning the 732-bp NotI fragment of Article Title: High level Purkinje cell specific expression of green fluorescent protein in transgenic mice. Article Snippet: The green fluorescent protein (GFP) has become a powerful tool in molecular and cell biology.. It is a commonly used marker for cloning and transfection experiments as well as a useful label of living cells allowing continuous observation of developing structures.. In order to unravel mechanisms of neuronal differentiation, we generated a transgenic mouse model which expresses GFPS65T,hu under the control of the Purkinje cellspecific promoter L7/pcp-2. Article Title: Green fluorescent protein causes mitochondria to aggregate in the presence of the Bcl-2 family proteins. Article Snippet: Green fluorescent protein (GFP) has been widely used in a variety of experiments in cell biology.. When cells were co-transfected with the GFP gene and the bcl-2 family genes bcl-2, bcl-xL, and bax, mitochondria appeared to aggregate at the periphery of the nucleus specifically where GFP was expressed.. Little aggregation was seen in the presence of other members of the GFP family, EGFP (enhanced GFP), ECFP (enhanced cyan variant), and EYFP (enhanced yellow-green variant). Expressing:Article Title: Methods for screening ligands that activate the translocation of a steroid receptor to the nucleus in mammalian cells Article Snippet: For example, Article Title: Transcriptional termination modulated by nucleotides outside the characterized gene end sequence of respiratory syncytial virus. Article Snippet: M11486 and M75730): the RS viral leader (A2 nt 1 to 44); the first 48 nt of the NS1 gene (A2 nt 45 to 92), with a single nt change at A2 nt 90 that resulted in the creation of an AvrII site; nucleotides 10 to 410 of the SH gene (A2 nt 4229 to 4628); the 44 nt SH/G intergenic region (A2 nt 4629 to 4672); the 9 nt G GS sequence (A2 nt 4673 to 4682); an inserted KpnI site; a 734-nt sequence containing the GFP open reading frame from Article Title: Distinct effect of pregnenolone sulfate on NMDA receptor subtypes Article Snippet: Using rapid agonist applications to transfected HEK-293 cells, we investigated pregnenolone sulfate (PS) effects on deactivation and desensitization of recombinant NMDA receptors subtypes.. PS prolonged the deactivation of responses produced by brief applications of L-glutamate with all subunit combinations tested.. The action of PS was larger on NR1a/NR2A than on NR1a/NR2B channels. Article Title: Chimeric protein comprising a green fluorescent ptotein fused to a transcription factor Article Snippet: For example, Article Title: Human ClC-3 Is Not the Swelling-activated Chloride Channel Involved in Cell Volume Regulation Article Snippet: To generate a cell line expressing short hClC-3 without a GFP tag, HEK293 cells were cotransfected with pGreenLantern-1, which encodes GFP ( Article Title: Proteinase-Polymerase Precursor as the Active Form of Feline Calicivirus RNA-Dependent RNA Polymerase Article Snippet: Plasmid pGLT7 was constructed by subcloning the 732-bp NotI fragment of Article Title: High level Purkinje cell specific expression of green fluorescent protein in transgenic mice. Article Snippet: The green fluorescent protein (GFP) has become a powerful tool in molecular and cell biology.. It is a commonly used marker for cloning and transfection experiments as well as a useful label of living cells allowing continuous observation of developing structures.. In order to unravel mechanisms of neuronal differentiation, we generated a transgenic mouse model which expresses GFPS65T,hu under the control of the Purkinje cellspecific promoter L7/pcp-2. Article Title: Green fluorescent protein causes mitochondria to aggregate in the presence of the Bcl-2 family proteins. Article Snippet: Green fluorescent protein (GFP) has been widely used in a variety of experiments in cell biology.. When cells were co-transfected with the GFP gene and the bcl-2 family genes bcl-2, bcl-xL, and bax, mitochondria appeared to aggregate at the periphery of the nucleus specifically where GFP was expressed.. Little aggregation was seen in the presence of other members of the GFP family, EGFP (enhanced GFP), ECFP (enhanced cyan variant), and EYFP (enhanced yellow-green variant). Plasmid Preparation:Article Title: Methods for screening ligands that activate the translocation of a steroid receptor to the nucleus in mammalian cells Article Snippet: For example, Article Title: Transcriptional termination modulated by nucleotides outside the characterized gene end sequence of respiratory syncytial virus. Article Snippet: M11486 and M75730): the RS viral leader (A2 nt 1 to 44); the first 48 nt of the NS1 gene (A2 nt 45 to 92), with a single nt change at A2 nt 90 that resulted in the creation of an AvrII site; nucleotides 10 to 410 of the SH gene (A2 nt 4229 to 4628); the 44 nt SH/G intergenic region (A2 nt 4629 to 4672); the 9 nt G GS sequence (A2 nt 4673 to 4682); an inserted KpnI site; a 734-nt sequence containing the GFP open reading frame from Article Title: Distinct effect of pregnenolone sulfate on NMDA receptor subtypes Article Snippet: Using rapid agonist applications to transfected HEK-293 cells, we investigated pregnenolone sulfate (PS) effects on deactivation and desensitization of recombinant NMDA receptors subtypes.. PS prolonged the deactivation of responses produced by brief applications of L-glutamate with all subunit combinations tested.. The action of PS was larger on NR1a/NR2A than on NR1a/NR2B channels. Article Title: Chimeric protein comprising a green fluorescent ptotein fused to a transcription factor Article Snippet: For example, Article Title: Human ClC-3 Is Not the Swelling-activated Chloride Channel Involved in Cell Volume Regulation Article Snippet: To generate a cell line expressing short hClC-3 without a GFP tag, HEK293 cells were cotransfected with pGreenLantern-1, which encodes GFP ( Article Title: Proteinase-Polymerase Precursor as the Active Form of Feline Calicivirus RNA-Dependent RNA Polymerase Article Snippet: Plasmid pGLT7 was constructed by subcloning the 732-bp NotI fragment of Article Title: High level Purkinje cell specific expression of green fluorescent protein in transgenic mice. Article Snippet: The green fluorescent protein (GFP) has become a powerful tool in molecular and cell biology.. It is a commonly used marker for cloning and transfection experiments as well as a useful label of living cells allowing continuous observation of developing structures.. In order to unravel mechanisms of neuronal differentiation, we generated a transgenic mouse model which expresses GFPS65T,hu under the control of the Purkinje cellspecific promoter L7/pcp-2. Article Title: Green fluorescent protein causes mitochondria to aggregate in the presence of the Bcl-2 family proteins. Article Snippet: Green fluorescent protein (GFP) has been widely used in a variety of experiments in cell biology.. When cells were co-transfected with the GFP gene and the bcl-2 family genes bcl-2, bcl-xL, and bax, mitochondria appeared to aggregate at the periphery of the nucleus specifically where GFP was expressed.. Little aggregation was seen in the presence of other members of the GFP family, EGFP (enhanced GFP), ECFP (enhanced cyan variant), and EYFP (enhanced yellow-green variant). Generated:Article Title: Methods for screening ligands that activate the translocation of a steroid receptor to the nucleus in mammalian cells Article Snippet: For example, Article Title: Transcriptional termination modulated by nucleotides outside the characterized gene end sequence of respiratory syncytial virus. Article Snippet: M11486 and M75730): the RS viral leader (A2 nt 1 to 44); the first 48 nt of the NS1 gene (A2 nt 45 to 92), with a single nt change at A2 nt 90 that resulted in the creation of an AvrII site; nucleotides 10 to 410 of the SH gene (A2 nt 4229 to 4628); the 44 nt SH/G intergenic region (A2 nt 4629 to 4672); the 9 nt G GS sequence (A2 nt 4673 to 4682); an inserted KpnI site; a 734-nt sequence containing the GFP open reading frame from Article Title: Distinct effect of pregnenolone sulfate on NMDA receptor subtypes Article Snippet: Using rapid agonist applications to transfected HEK-293 cells, we investigated pregnenolone sulfate (PS) effects on deactivation and desensitization of recombinant NMDA receptors subtypes.. PS prolonged the deactivation of responses produced by brief applications of L-glutamate with all subunit combinations tested.. The action of PS was larger on NR1a/NR2A than on NR1a/NR2B channels. Article Title: Chimeric protein comprising a green fluorescent ptotein fused to a transcription factor Article Snippet: For example, Article Title: Human ClC-3 Is Not the Swelling-activated Chloride Channel Involved in Cell Volume Regulation Article Snippet: To generate a cell line expressing short hClC-3 without a GFP tag, HEK293 cells were cotransfected with pGreenLantern-1, which encodes GFP ( Article Title: Proteinase-Polymerase Precursor as the Active Form of Feline Calicivirus RNA-Dependent RNA Polymerase Article Snippet: Plasmid pGLT7 was constructed by subcloning the 732-bp NotI fragment of Article Title: High level Purkinje cell specific expression of green fluorescent protein in transgenic mice. Article Snippet: The green fluorescent protein (GFP) has become a powerful tool in molecular and cell biology.. It is a commonly used marker for cloning and transfection experiments as well as a useful label of living cells allowing continuous observation of developing structures.. In order to unravel mechanisms of neuronal differentiation, we generated a transgenic mouse model which expresses GFPS65T,hu under the control of the Purkinje cellspecific promoter L7/pcp-2. Article Title: Green fluorescent protein causes mitochondria to aggregate in the presence of the Bcl-2 family proteins. Article Snippet: Green fluorescent protein (GFP) has been widely used in a variety of experiments in cell biology.. When cells were co-transfected with the GFP gene and the bcl-2 family genes bcl-2, bcl-xL, and bax, mitochondria appeared to aggregate at the periphery of the nucleus specifically where GFP was expressed.. Little aggregation was seen in the presence of other members of the GFP family, EGFP (enhanced GFP), ECFP (enhanced cyan variant), and EYFP (enhanced yellow-green variant). Construct:Article Title: Methods for screening ligands that activate the translocation of a steroid receptor to the nucleus in mammalian cells Article Snippet: For example, Article Title: Transcriptional termination modulated by nucleotides outside the characterized gene end sequence of respiratory syncytial virus. Article Snippet: M11486 and M75730): the RS viral leader (A2 nt 1 to 44); the first 48 nt of the NS1 gene (A2 nt 45 to 92), with a single nt change at A2 nt 90 that resulted in the creation of an AvrII site; nucleotides 10 to 410 of the SH gene (A2 nt 4229 to 4628); the 44 nt SH/G intergenic region (A2 nt 4629 to 4672); the 9 nt G GS sequence (A2 nt 4673 to 4682); an inserted KpnI site; a 734-nt sequence containing the GFP open reading frame from Article Title: Distinct effect of pregnenolone sulfate on NMDA receptor subtypes Article Snippet: Using rapid agonist applications to transfected HEK-293 cells, we investigated pregnenolone sulfate (PS) effects on deactivation and desensitization of recombinant NMDA receptors subtypes.. PS prolonged the deactivation of responses produced by brief applications of L-glutamate with all subunit combinations tested.. The action of PS was larger on NR1a/NR2A than on NR1a/NR2B channels. Article Title: Chimeric protein comprising a green fluorescent ptotein fused to a transcription factor Article Snippet: For example, Article Title: Human ClC-3 Is Not the Swelling-activated Chloride Channel Involved in Cell Volume Regulation Article Snippet: To generate a cell line expressing short hClC-3 without a GFP tag, HEK293 cells were cotransfected with pGreenLantern-1, which encodes GFP ( Article Title: Proteinase-Polymerase Precursor as the Active Form of Feline Calicivirus RNA-Dependent RNA Polymerase Article Snippet: Plasmid pGLT7 was constructed by subcloning the 732-bp NotI fragment of Article Title: High level Purkinje cell specific expression of green fluorescent protein in transgenic mice. Article Snippet: The green fluorescent protein (GFP) has become a powerful tool in molecular and cell biology.. It is a commonly used marker for cloning and transfection experiments as well as a useful label of living cells allowing continuous observation of developing structures.. In order to unravel mechanisms of neuronal differentiation, we generated a transgenic mouse model which expresses GFPS65T,hu under the control of the Purkinje cellspecific promoter L7/pcp-2. Article Title: Green fluorescent protein causes mitochondria to aggregate in the presence of the Bcl-2 family proteins. Article Snippet: Green fluorescent protein (GFP) has been widely used in a variety of experiments in cell biology.. When cells were co-transfected with the GFP gene and the bcl-2 family genes bcl-2, bcl-xL, and bax, mitochondria appeared to aggregate at the periphery of the nucleus specifically where GFP was expressed.. Little aggregation was seen in the presence of other members of the GFP family, EGFP (enhanced GFP), ECFP (enhanced cyan variant), and EYFP (enhanced yellow-green variant). Cotransfection:Article Title: Methods for screening ligands that activate the translocation of a steroid receptor to the nucleus in mammalian cells Article Snippet: For example, Article Title: Transcriptional termination modulated by nucleotides outside the characterized gene end sequence of respiratory syncytial virus. Article Snippet: M11486 and M75730): the RS viral leader (A2 nt 1 to 44); the first 48 nt of the NS1 gene (A2 nt 45 to 92), with a single nt change at A2 nt 90 that resulted in the creation of an AvrII site; nucleotides 10 to 410 of the SH gene (A2 nt 4229 to 4628); the 44 nt SH/G intergenic region (A2 nt 4629 to 4672); the 9 nt G GS sequence (A2 nt 4673 to 4682); an inserted KpnI site; a 734-nt sequence containing the GFP open reading frame from Article Title: Distinct effect of pregnenolone sulfate on NMDA receptor subtypes Article Snippet: Using rapid agonist applications to transfected HEK-293 cells, we investigated pregnenolone sulfate (PS) effects on deactivation and desensitization of recombinant NMDA receptors subtypes.. PS prolonged the deactivation of responses produced by brief applications of L-glutamate with all subunit combinations tested.. The action of PS was larger on NR1a/NR2A than on NR1a/NR2B channels. Article Title: Chimeric protein comprising a green fluorescent ptotein fused to a transcription factor Article Snippet: For example, Article Title: Human ClC-3 Is Not the Swelling-activated Chloride Channel Involved in Cell Volume Regulation Article Snippet: To generate a cell line expressing short hClC-3 without a GFP tag, HEK293 cells were cotransfected with pGreenLantern-1, which encodes GFP ( Article Title: Proteinase-Polymerase Precursor as the Active Form of Feline Calicivirus RNA-Dependent RNA Polymerase Article Snippet: Plasmid pGLT7 was constructed by subcloning the 732-bp NotI fragment of Article Title: High level Purkinje cell specific expression of green fluorescent protein in transgenic mice. Article Snippet: The green fluorescent protein (GFP) has become a powerful tool in molecular and cell biology.. It is a commonly used marker for cloning and transfection experiments as well as a useful label of living cells allowing continuous observation of developing structures.. In order to unravel mechanisms of neuronal differentiation, we generated a transgenic mouse model which expresses GFPS65T,hu under the control of the Purkinje cellspecific promoter L7/pcp-2. Article Title: Green fluorescent protein causes mitochondria to aggregate in the presence of the Bcl-2 family proteins. Article Snippet: Green fluorescent protein (GFP) has been widely used in a variety of experiments in cell biology.. When cells were co-transfected with the GFP gene and the bcl-2 family genes bcl-2, bcl-xL, and bax, mitochondria appeared to aggregate at the periphery of the nucleus specifically where GFP was expressed.. Little aggregation was seen in the presence of other members of the GFP family, EGFP (enhanced GFP), ECFP (enhanced cyan variant), and EYFP (enhanced yellow-green variant). Transfection:Article Title: Methods for screening ligands that activate the translocation of a steroid receptor to the nucleus in mammalian cells Article Snippet: For example, Article Title: Transcriptional termination modulated by nucleotides outside the characterized gene end sequence of respiratory syncytial virus. Article Snippet: M11486 and M75730): the RS viral leader (A2 nt 1 to 44); the first 48 nt of the NS1 gene (A2 nt 45 to 92), with a single nt change at A2 nt 90 that resulted in the creation of an AvrII site; nucleotides 10 to 410 of the SH gene (A2 nt 4229 to 4628); the 44 nt SH/G intergenic region (A2 nt 4629 to 4672); the 9 nt G GS sequence (A2 nt 4673 to 4682); an inserted KpnI site; a 734-nt sequence containing the GFP open reading frame from Article Title: Distinct effect of pregnenolone sulfate on NMDA receptor subtypes Article Snippet: Using rapid agonist applications to transfected HEK-293 cells, we investigated pregnenolone sulfate (PS) effects on deactivation and desensitization of recombinant NMDA receptors subtypes.. PS prolonged the deactivation of responses produced by brief applications of L-glutamate with all subunit combinations tested.. The action of PS was larger on NR1a/NR2A than on NR1a/NR2B channels. Article Title: Chimeric protein comprising a green fluorescent ptotein fused to a transcription factor Article Snippet: For example, Article Title: Human ClC-3 Is Not the Swelling-activated Chloride Channel Involved in Cell Volume Regulation Article Snippet: To generate a cell line expressing short hClC-3 without a GFP tag, HEK293 cells were cotransfected with pGreenLantern-1, which encodes GFP ( Article Title: Proteinase-Polymerase Precursor as the Active Form of Feline Calicivirus RNA-Dependent RNA Polymerase Article Snippet: Plasmid pGLT7 was constructed by subcloning the 732-bp NotI fragment of Article Title: High level Purkinje cell specific expression of green fluorescent protein in transgenic mice. Article Snippet: The green fluorescent protein (GFP) has become a powerful tool in molecular and cell biology.. It is a commonly used marker for cloning and transfection experiments as well as a useful label of living cells allowing continuous observation of developing structures.. In order to unravel mechanisms of neuronal differentiation, we generated a transgenic mouse model which expresses GFPS65T,hu under the control of the Purkinje cellspecific promoter L7/pcp-2. Article Title: Green fluorescent protein causes mitochondria to aggregate in the presence of the Bcl-2 family proteins. Article Snippet: Green fluorescent protein (GFP) has been widely used in a variety of experiments in cell biology.. When cells were co-transfected with the GFP gene and the bcl-2 family genes bcl-2, bcl-xL, and bax, mitochondria appeared to aggregate at the periphery of the nucleus specifically where GFP was expressed.. Little aggregation was seen in the presence of other members of the GFP family, EGFP (enhanced GFP), ECFP (enhanced cyan variant), and EYFP (enhanced yellow-green variant). Subcloning:Article Title: Methods for screening ligands that activate the translocation of a steroid receptor to the nucleus in mammalian cells Article Snippet: For example, Article Title: Transcriptional termination modulated by nucleotides outside the characterized gene end sequence of respiratory syncytial virus. Article Snippet: M11486 and M75730): the RS viral leader (A2 nt 1 to 44); the first 48 nt of the NS1 gene (A2 nt 45 to 92), with a single nt change at A2 nt 90 that resulted in the creation of an AvrII site; nucleotides 10 to 410 of the SH gene (A2 nt 4229 to 4628); the 44 nt SH/G intergenic region (A2 nt 4629 to 4672); the 9 nt G GS sequence (A2 nt 4673 to 4682); an inserted KpnI site; a 734-nt sequence containing the GFP open reading frame from Article Title: Distinct effect of pregnenolone sulfate on NMDA receptor subtypes Article Snippet: Using rapid agonist applications to transfected HEK-293 cells, we investigated pregnenolone sulfate (PS) effects on deactivation and desensitization of recombinant NMDA receptors subtypes.. PS prolonged the deactivation of responses produced by brief applications of L-glutamate with all subunit combinations tested.. The action of PS was larger on NR1a/NR2A than on NR1a/NR2B channels. Article Title: Chimeric protein comprising a green fluorescent ptotein fused to a transcription factor Article Snippet: For example, Article Title: Human ClC-3 Is Not the Swelling-activated Chloride Channel Involved in Cell Volume Regulation Article Snippet: To generate a cell line expressing short hClC-3 without a GFP tag, HEK293 cells were cotransfected with pGreenLantern-1, which encodes GFP ( Article Title: Proteinase-Polymerase Precursor as the Active Form of Feline Calicivirus RNA-Dependent RNA Polymerase Article Snippet: Plasmid pGLT7 was constructed by subcloning the 732-bp NotI fragment of Article Title: High level Purkinje cell specific expression of green fluorescent protein in transgenic mice. Article Snippet: The green fluorescent protein (GFP) has become a powerful tool in molecular and cell biology.. It is a commonly used marker for cloning and transfection experiments as well as a useful label of living cells allowing continuous observation of developing structures.. In order to unravel mechanisms of neuronal differentiation, we generated a transgenic mouse model which expresses GFPS65T,hu under the control of the Purkinje cellspecific promoter L7/pcp-2. Article Title: Green fluorescent protein causes mitochondria to aggregate in the presence of the Bcl-2 family proteins. Article Snippet: Green fluorescent protein (GFP) has been widely used in a variety of experiments in cell biology.. When cells were co-transfected with the GFP gene and the bcl-2 family genes bcl-2, bcl-xL, and bax, mitochondria appeared to aggregate at the periphery of the nucleus specifically where GFP was expressed.. Little aggregation was seen in the presence of other members of the GFP family, EGFP (enhanced GFP), ECFP (enhanced cyan variant), and EYFP (enhanced yellow-green variant). |