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Promega pgl3-basic luciferase reporter vectors
Pgl3 Basic Luciferase Reporter Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3-basic+luciferase+reporter+vector/pgl3+basic/pmc12261028-74-13-17
Average 90 stars, based on 1 article reviews
pgl3-basic luciferase reporter vectors - by Bioz Stars, 2026-09
90/100 stars

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Polymerase Chain Reaction:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Clone Assay:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Luciferase:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Plasmid Preparation:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Functional Assay:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Expressing:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Transfection:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Activity Assay:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Construct:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Electrophoretic Mobility Shift Assay:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Labeling:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Incubation:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Binding Assay:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Sequencing:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Amplification:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Reporter Assay:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Mutagenesis:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).

Modification:

Article Title: Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.
Article Snippet: For FNIP1 and FNIP2 Luciferase Reporter assay, the predicted MEF2 DNA binding sites on FNIP1 and FNIP2 promoter regions were amplified by PCR from the HeLa cell genome and cloned into KpnI and XhoI sites of pGL3-basic luciferase reporter vector (Promega, E1751).

Article Title: Granulosa cell-layer stiffening prevents the granulosa cells from escaping the post-ovulatory follicle
Article Snippet: To construct the reporter vectors, the promoter regions of VCL and TLN1 were amplified and inserted into the PGL3-Basic luciferase reporter vector (Promega, USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme, China).

Article Title: Genetic association and functional implications of TLR4 rs1927914 polymorphism on colon cancer risk
Article Snippet: The PCR product was digested by Kpn I and Nhe I and then cloned into pGL3-basic luciferase reporter vector (Promega, Madison, USA).

Article Title: HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense www.advancedsciencenews.com www.advancedscience.com were amplified and cloned into the pGL3-basic luciferase reporter vector (Promega).

Article Title: Exosomal miRNA-166-5p derived from G-MDSCs promotes proliferation by targeting ITM3E in colorectal cancer.
Article Snippet: Correspondence Haoping Xu, Department of Radiation Oncology, Ruijin Hospital Affilated to School of Medicine, Shanghai Jiaotong University, Ruijin Er Road No. 197, Shanghai 200025, China.. Email: xhp11701@rjh.com.cn Abstract Background: The immune milieu of colorectal cancer is a complex phenomenon.. It is imperative to investigate the crucial immune factors that promote the progression of colorectal cancer.

Article Title: Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response
Article Snippet: The plasmids containing zebrafish IFNφ1pro-Luc and ISRE-Luc in the pGL3-Basic luciferase reporter vector (Promega) were constructed as described previously.

Article Title: Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regeneration.
Article Snippet: The FOXA3 expression plasmid and Foxa3-luciferase reporter were constructed as previously described.24 The Cebpb promoter was amplified from mouse genomic DNA and inserted to the pGL3-basic luciferase reporter vector (E1751; Promega, Madison, WI, USA).

Article Title: SPINK1-induced tumor plasticity provides a therapeutic window for chemotherapy in hepatocellular carcinoma.
Article Snippet: The full-length SPINK1 promoter was amplified from the HCC cell line Huh7 and cloned into the pGL3-Basic luciferase reporter vector (Promega) using the primers (F: 5’-AAAGAGCTCGGGCAACCCT TTCTTTGTGG-3’; R: 5’-AAAACGCGTCCGCACTTACCACGTCTCTT-3’). pT1 (ΔS2), pT2 (ΔS2) and pT3 (ΔS1 +ΔS2) were purchased from Guangzhou IGE Biotechnology Ltd. Luciferase reporter constructs and pRL-CMV Renilla luciferase control were transiently co-transfected intoHCCcell lines by Lipofectamine 3000 reagent (ThermoScientific).



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MAD2 and BUB1B are transcriptionally activated by ERα. (A) Schematic representations showing the estrogen response elements (EREs) in the promoter regions of MAD2 and BUB1B . (B) E2 (17β-estradiol) treatment up-regulates MAD2 and BUB1B promoter activity in MCF-7 cells. The top panel shows the map of MAD2 and BUB1B promoter regions used for <t>luciferase</t> reporter assay. pSS-MAD2 construct contains an 1128-bp region (−1098 to +30 nt) of the MAD2 gene and the pSS-BUB1B construct contains a 1546-bp region (−1454 to +92 nt) of the BUB1B gene, cloned into <t>pGL3</t> basic vector. MCF-7 cells were transiently transfected with pSS-MAD2 (250 ng) and pSS-BUB1B (500 ng), followed by E2 treatment, and proceeded for luciferase reporter assay. **p < 0.005; (two-tailed unpaired Student’s t-test) (C) Ectopic ERα up-regulates MAD2 and BUB1B promoter activity in MDA-MB-231 cells. MDA-MB-231 cells were co-transfected with pSS-MAD2 (250 ng) and pSS-BUB1B (500 ng) along with 1.5 µg of ectopic ERα (pCMV3-N-FLAG-ESR1), followed by E2 treatment and proceeded for luciferase reporter assay and plotted as discussed in (B). *p < 0.05; **p < 0.005; ***p < 0.0005 (two-tailed unpaired Student’s t-test). (D) Deletion of ERE results in a significant decrease in luciferase activity. Map of the two deletion constructs, ΔERE pSS-MAD2, and ΔERE pSS-BUB1B were shown in the top panel. MCF-7 cells were transfected with either ΔERE pSS-MAD2 (250 ng) or ΔERE pSS-BUB1B (500 ng), followed by E2 treatment. Data were plotted as discussed in (B). **p < 0.005; ns, not significant (two-tailed unpaired Student’s t-test). (E) ERα physically recruits to MAD2 and BUB1B promoters. The total cell extract of MCF-7 was prepared and Chromatin immunoprecipitation was done using antibodies specific for ERα and normal IgG as control. Precipitated chromatin was estimated by qRT-PCR. The results are expressed as a percent of input. *p < 0.05; **p < 0.005; (two-tailed unpaired Student’s t-test). (F) Representative scatter plot depicting the Pearson’s correlation analysis between the gene expression values (TPM) of MAD2 and BUB1B , extracted from TCGA BRCA cohort using UCSC Xena database. The Pearson correlation coefficient is reported as the ‘r’ value. ** *p < 0.0005; (two-tailed unpaired Student’s t-test). All the experiments (B-E) were performed in triplicates and error bars were represented as means ± s.d.
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MAD2 and BUB1B are transcriptionally activated by ERα. (A) Schematic representations showing the estrogen response elements (EREs) in the promoter regions of MAD2 and BUB1B . (B) E2 (17β-estradiol) treatment up-regulates MAD2 and BUB1B promoter activity in MCF-7 cells. The top panel shows the map of MAD2 and BUB1B promoter regions used for luciferase reporter assay. pSS-MAD2 construct contains an 1128-bp region (−1098 to +30 nt) of the MAD2 gene and the pSS-BUB1B construct contains a 1546-bp region (−1454 to +92 nt) of the BUB1B gene, cloned into pGL3 basic vector. MCF-7 cells were transiently transfected with pSS-MAD2 (250 ng) and pSS-BUB1B (500 ng), followed by E2 treatment, and proceeded for luciferase reporter assay. **p < 0.005; (two-tailed unpaired Student’s t-test) (C) Ectopic ERα up-regulates MAD2 and BUB1B promoter activity in MDA-MB-231 cells. MDA-MB-231 cells were co-transfected with pSS-MAD2 (250 ng) and pSS-BUB1B (500 ng) along with 1.5 µg of ectopic ERα (pCMV3-N-FLAG-ESR1), followed by E2 treatment and proceeded for luciferase reporter assay and plotted as discussed in (B). *p < 0.05; **p < 0.005; ***p < 0.0005 (two-tailed unpaired Student’s t-test). (D) Deletion of ERE results in a significant decrease in luciferase activity. Map of the two deletion constructs, ΔERE pSS-MAD2, and ΔERE pSS-BUB1B were shown in the top panel. MCF-7 cells were transfected with either ΔERE pSS-MAD2 (250 ng) or ΔERE pSS-BUB1B (500 ng), followed by E2 treatment. Data were plotted as discussed in (B). **p < 0.005; ns, not significant (two-tailed unpaired Student’s t-test). (E) ERα physically recruits to MAD2 and BUB1B promoters. The total cell extract of MCF-7 was prepared and Chromatin immunoprecipitation was done using antibodies specific for ERα and normal IgG as control. Precipitated chromatin was estimated by qRT-PCR. The results are expressed as a percent of input. *p < 0.05; **p < 0.005; (two-tailed unpaired Student’s t-test). (F) Representative scatter plot depicting the Pearson’s correlation analysis between the gene expression values (TPM) of MAD2 and BUB1B , extracted from TCGA BRCA cohort using UCSC Xena database. The Pearson correlation coefficient is reported as the ‘r’ value. ** *p < 0.0005; (two-tailed unpaired Student’s t-test). All the experiments (B-E) were performed in triplicates and error bars were represented as means ± s.d.

Journal: bioRxiv

Article Title: Estrogen receptor alpha (ERα) driven trans-regulation of mitotic checkpoint complex (MCC) components affects the clinical outcome of breast cancer

doi: 10.1101/2024.08.31.610592

Figure Lengend Snippet: MAD2 and BUB1B are transcriptionally activated by ERα. (A) Schematic representations showing the estrogen response elements (EREs) in the promoter regions of MAD2 and BUB1B . (B) E2 (17β-estradiol) treatment up-regulates MAD2 and BUB1B promoter activity in MCF-7 cells. The top panel shows the map of MAD2 and BUB1B promoter regions used for luciferase reporter assay. pSS-MAD2 construct contains an 1128-bp region (−1098 to +30 nt) of the MAD2 gene and the pSS-BUB1B construct contains a 1546-bp region (−1454 to +92 nt) of the BUB1B gene, cloned into pGL3 basic vector. MCF-7 cells were transiently transfected with pSS-MAD2 (250 ng) and pSS-BUB1B (500 ng), followed by E2 treatment, and proceeded for luciferase reporter assay. **p < 0.005; (two-tailed unpaired Student’s t-test) (C) Ectopic ERα up-regulates MAD2 and BUB1B promoter activity in MDA-MB-231 cells. MDA-MB-231 cells were co-transfected with pSS-MAD2 (250 ng) and pSS-BUB1B (500 ng) along with 1.5 µg of ectopic ERα (pCMV3-N-FLAG-ESR1), followed by E2 treatment and proceeded for luciferase reporter assay and plotted as discussed in (B). *p < 0.05; **p < 0.005; ***p < 0.0005 (two-tailed unpaired Student’s t-test). (D) Deletion of ERE results in a significant decrease in luciferase activity. Map of the two deletion constructs, ΔERE pSS-MAD2, and ΔERE pSS-BUB1B were shown in the top panel. MCF-7 cells were transfected with either ΔERE pSS-MAD2 (250 ng) or ΔERE pSS-BUB1B (500 ng), followed by E2 treatment. Data were plotted as discussed in (B). **p < 0.005; ns, not significant (two-tailed unpaired Student’s t-test). (E) ERα physically recruits to MAD2 and BUB1B promoters. The total cell extract of MCF-7 was prepared and Chromatin immunoprecipitation was done using antibodies specific for ERα and normal IgG as control. Precipitated chromatin was estimated by qRT-PCR. The results are expressed as a percent of input. *p < 0.05; **p < 0.005; (two-tailed unpaired Student’s t-test). (F) Representative scatter plot depicting the Pearson’s correlation analysis between the gene expression values (TPM) of MAD2 and BUB1B , extracted from TCGA BRCA cohort using UCSC Xena database. The Pearson correlation coefficient is reported as the ‘r’ value. ** *p < 0.0005; (two-tailed unpaired Student’s t-test). All the experiments (B-E) were performed in triplicates and error bars were represented as means ± s.d.

Article Snippet: The fragments were then subcloned into luciferase reporter vector pGL3 basic (Promega) using restriction enzyme BglII (New England Biolabs).

Techniques: Activity Assay, Luciferase, Reporter Assay, Construct, Clone Assay, Plasmid Preparation, Transfection, Two Tailed Test, Chromatin Immunoprecipitation, Control, Quantitative RT-PCR, Expressing