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vector pgl3  (Addgene inc)


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    Addgene inc vector pgl3
    Vector Pgl3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3+basic+luciferase+vector/pGL3+(-BRE)+Luciferase+(Plasmid+%2345127)/pm40876500-286-24-29
    Average 95 stars, based on 195 article reviews
    vector pgl3 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Amplification:

    Article Title: MAZ-mediated LAMA5 transcription activation promotes gastric cancer progression through the STAT3 signaling.
    Article Snippet: .. Sequences of amplified LAMA5 promoter fragments or LAMA5 promoter mutants (LAMA5 pro-Mut, chr20:62367260–62367270, chr20:62367356–623673366, chr20:62367418–623673428) were digested by KpnI and XhoI and ligated to pGL3 basic luciferase vector (212936, Addgene, Watertown, MA, USA) to construct the target plasmid. .. The constructed target plasmids were subsequently co-transfected with pRL Renilla Luciferase Control Reporter Vectors (E2241, Promega Corporation, Madison, 1 3 59 Page 4 of 15 Functional & Integrative Genomics (2025) 25:59 We detected the expression of LAMA5 in GES1 cells, and human GC cells AGS and HGC27 using RT-qPCR and western blot analysis.

    Article Title: MAZ-mediated LAMA5 transcription activation promotes gastric cancer progression through the STAT3 signaling
    Article Snippet: .. Sequences of amplified LAMA5 promoter fragments or LAMA5 promoter mutants (LAMA5 pro-Mut, chr20:62367260–62367270, chr20:62367356–623673366, chr20:62367418–623673428) were digested by KpnI and XhoI and ligated to pGL3 basic luciferase vector (212936, Addgene, Watertown, MA, USA) to construct the target plasmid. .. The constructed target plasmids were subsequently co-transfected with pRL Renilla Luciferase Control Reporter Vectors (E2241, Promega Corporation, Madison, WI, USA) into sh-NC and sh-MAZ treated GC cells.

    Luciferase:

    Article Title: MAZ-mediated LAMA5 transcription activation promotes gastric cancer progression through the STAT3 signaling.
    Article Snippet: .. Sequences of amplified LAMA5 promoter fragments or LAMA5 promoter mutants (LAMA5 pro-Mut, chr20:62367260–62367270, chr20:62367356–623673366, chr20:62367418–623673428) were digested by KpnI and XhoI and ligated to pGL3 basic luciferase vector (212936, Addgene, Watertown, MA, USA) to construct the target plasmid. .. The constructed target plasmids were subsequently co-transfected with pRL Renilla Luciferase Control Reporter Vectors (E2241, Promega Corporation, Madison, 1 3 59 Page 4 of 15 Functional & Integrative Genomics (2025) 25:59 We detected the expression of LAMA5 in GES1 cells, and human GC cells AGS and HGC27 using RT-qPCR and western blot analysis.

    Article Title: MAZ-mediated LAMA5 transcription activation promotes gastric cancer progression through the STAT3 signaling
    Article Snippet: .. Sequences of amplified LAMA5 promoter fragments or LAMA5 promoter mutants (LAMA5 pro-Mut, chr20:62367260–62367270, chr20:62367356–623673366, chr20:62367418–623673428) were digested by KpnI and XhoI and ligated to pGL3 basic luciferase vector (212936, Addgene, Watertown, MA, USA) to construct the target plasmid. .. The constructed target plasmids were subsequently co-transfected with pRL Renilla Luciferase Control Reporter Vectors (E2241, Promega Corporation, Madison, WI, USA) into sh-NC and sh-MAZ treated GC cells.

    Article Title: "MiR-7 controls cholesterol biosynthesis through posttranscriptional regulation of DHCR24 expression".
    Article Snippet: Dysregulation of cholesterol homeostasis is associated with several pathologies including cardiovascular diseases and neurological disorders such as Alzheimer’s disease (AD).. MicroRNAs (miRNAs) have emerged as key posttranscriptional regulators of cholesterol metabolism.. We previously established the role of miR-7 in regulating insulin resistance and amyloidosis, which represents a common pathological feature between type 2 diabetes and AD.

    Construct:

    Article Title: MAZ-mediated LAMA5 transcription activation promotes gastric cancer progression through the STAT3 signaling.
    Article Snippet: .. Sequences of amplified LAMA5 promoter fragments or LAMA5 promoter mutants (LAMA5 pro-Mut, chr20:62367260–62367270, chr20:62367356–623673366, chr20:62367418–623673428) were digested by KpnI and XhoI and ligated to pGL3 basic luciferase vector (212936, Addgene, Watertown, MA, USA) to construct the target plasmid. .. The constructed target plasmids were subsequently co-transfected with pRL Renilla Luciferase Control Reporter Vectors (E2241, Promega Corporation, Madison, 1 3 59 Page 4 of 15 Functional & Integrative Genomics (2025) 25:59 We detected the expression of LAMA5 in GES1 cells, and human GC cells AGS and HGC27 using RT-qPCR and western blot analysis.

    Article Title: MAZ-mediated LAMA5 transcription activation promotes gastric cancer progression through the STAT3 signaling
    Article Snippet: .. Sequences of amplified LAMA5 promoter fragments or LAMA5 promoter mutants (LAMA5 pro-Mut, chr20:62367260–62367270, chr20:62367356–623673366, chr20:62367418–623673428) were digested by KpnI and XhoI and ligated to pGL3 basic luciferase vector (212936, Addgene, Watertown, MA, USA) to construct the target plasmid. .. The constructed target plasmids were subsequently co-transfected with pRL Renilla Luciferase Control Reporter Vectors (E2241, Promega Corporation, Madison, WI, USA) into sh-NC and sh-MAZ treated GC cells.

    Article Title: "MiR-7 controls cholesterol biosynthesis through posttranscriptional regulation of DHCR24 expression".
    Article Snippet: Dysregulation of cholesterol homeostasis is associated with several pathologies including cardiovascular diseases and neurological disorders such as Alzheimer’s disease (AD).. MicroRNAs (miRNAs) have emerged as key posttranscriptional regulators of cholesterol metabolism.. We previously established the role of miR-7 in regulating insulin resistance and amyloidosis, which represents a common pathological feature between type 2 diabetes and AD.

    Plasmid Preparation:

    Article Title: MAZ-mediated LAMA5 transcription activation promotes gastric cancer progression through the STAT3 signaling.
    Article Snippet: .. Sequences of amplified LAMA5 promoter fragments or LAMA5 promoter mutants (LAMA5 pro-Mut, chr20:62367260–62367270, chr20:62367356–623673366, chr20:62367418–623673428) were digested by KpnI and XhoI and ligated to pGL3 basic luciferase vector (212936, Addgene, Watertown, MA, USA) to construct the target plasmid. .. The constructed target plasmids were subsequently co-transfected with pRL Renilla Luciferase Control Reporter Vectors (E2241, Promega Corporation, Madison, 1 3 59 Page 4 of 15 Functional & Integrative Genomics (2025) 25:59 We detected the expression of LAMA5 in GES1 cells, and human GC cells AGS and HGC27 using RT-qPCR and western blot analysis.

    Article Title: MAZ-mediated LAMA5 transcription activation promotes gastric cancer progression through the STAT3 signaling
    Article Snippet: .. Sequences of amplified LAMA5 promoter fragments or LAMA5 promoter mutants (LAMA5 pro-Mut, chr20:62367260–62367270, chr20:62367356–623673366, chr20:62367418–623673428) were digested by KpnI and XhoI and ligated to pGL3 basic luciferase vector (212936, Addgene, Watertown, MA, USA) to construct the target plasmid. .. The constructed target plasmids were subsequently co-transfected with pRL Renilla Luciferase Control Reporter Vectors (E2241, Promega Corporation, Madison, WI, USA) into sh-NC and sh-MAZ treated GC cells.

    Article Title: "MiR-7 controls cholesterol biosynthesis through posttranscriptional regulation of DHCR24 expression".
    Article Snippet: Dysregulation of cholesterol homeostasis is associated with several pathologies including cardiovascular diseases and neurological disorders such as Alzheimer’s disease (AD).. MicroRNAs (miRNAs) have emerged as key posttranscriptional regulators of cholesterol metabolism.. We previously established the role of miR-7 in regulating insulin resistance and amyloidosis, which represents a common pathological feature between type 2 diabetes and AD.



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    Image Search Results


    (a) RT-qPCR of ILF3 mRNA levels in SH-SY5Y cells overexpressing GFP-ZNF184. (b) Immunoblot analysis of ILF3 protein levels in the GFP-ZNF184-overexpressing SH-SY5Y cells. ZNF184 antibody detects both endogenous ZNF184 (indicated by a single red asterisk) and exogenous GFP-tagged ZNF184 (indicated by two red asterisks). Quantification of ILF3 protein levels is normalized to β-actin. (c) Immunoblotting of ILF3 and ZNF184 in ZNF184 knockdown SH-SY5Y cells by siRNA-ZNF184. Quantification of the immunoblot normalized to β-actin. (d) Prediction of ZNF184 consensus sequence by expanding linear SVM. (e) ChIP assay of Flag-tagged ZNF184-overexpressing SH-SY5Y cells. Rabbit IgG and histone antibodies are used as negative and positive controls, respectively. (f) Localization of ZNF184 ChIP primer binding site in the human ILF3 promoter with ZNF184 consensus sequence, human ILF3 promoter (WT) sequence, MT1 (mutant 1), MT2 (mutant 2), and MT1/2 (mutant 1 and 2) sequences. (g) Luciferase assay of overexpressing SH-SY5Y cells with pGL3-basic vector, pGL3-hILF3 promoter (WT), mutants (MT1, MT2, and MT1/2) and Flag- ZNF184. (h, i) Immunoblotting of ILF3 in CTX (h) and SN (i) samples of a human patient with PD ( n = 3/group). Statistical significance was assessed through a one-way ANOVA and unpaired t- test, with significance levels defined as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Journal: PLOS One

    Article Title: Zinc finger protein 184 prevents α-synuclein preformed fibril-mediated neurodegeneration through the interleukin enhancer binding factor 3-microRNA-7 pathway

    doi: 10.1371/journal.pone.0323279

    Figure Lengend Snippet: (a) RT-qPCR of ILF3 mRNA levels in SH-SY5Y cells overexpressing GFP-ZNF184. (b) Immunoblot analysis of ILF3 protein levels in the GFP-ZNF184-overexpressing SH-SY5Y cells. ZNF184 antibody detects both endogenous ZNF184 (indicated by a single red asterisk) and exogenous GFP-tagged ZNF184 (indicated by two red asterisks). Quantification of ILF3 protein levels is normalized to β-actin. (c) Immunoblotting of ILF3 and ZNF184 in ZNF184 knockdown SH-SY5Y cells by siRNA-ZNF184. Quantification of the immunoblot normalized to β-actin. (d) Prediction of ZNF184 consensus sequence by expanding linear SVM. (e) ChIP assay of Flag-tagged ZNF184-overexpressing SH-SY5Y cells. Rabbit IgG and histone antibodies are used as negative and positive controls, respectively. (f) Localization of ZNF184 ChIP primer binding site in the human ILF3 promoter with ZNF184 consensus sequence, human ILF3 promoter (WT) sequence, MT1 (mutant 1), MT2 (mutant 2), and MT1/2 (mutant 1 and 2) sequences. (g) Luciferase assay of overexpressing SH-SY5Y cells with pGL3-basic vector, pGL3-hILF3 promoter (WT), mutants (MT1, MT2, and MT1/2) and Flag- ZNF184. (h, i) Immunoblotting of ILF3 in CTX (h) and SN (i) samples of a human patient with PD ( n = 3/group). Statistical significance was assessed through a one-way ANOVA and unpaired t- test, with significance levels defined as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Article Snippet: SH-SY5Y cells were transfected for 48 h using the pGL3-basic vector (Addgene, Watertown, MA, USA), pGL3-human ILF3 promoter (pGL3-hILF3), Flag-ZNF184, pGL3-MT1, pGL3-MT2, and pGL3-MT1/2 in Opti-MEM (Gibco, Grand Island, NY, USA) with the Xtreme-gene (Roche Holding AG, Basel, Switzerland).

    Techniques: Quantitative RT-PCR, Western Blot, Knockdown, Sequencing, Binding Assay, Mutagenesis, Luciferase, Plasmid Preparation

    ( A ) Schematic of the human JMJD3 gene, including gene structure, H3K27Ac, DHS, BP-associated SNPs, cloned fragments for luciferase assays, and vertebrate conservation (UCSC genome browser). ( B ) Luciferase results of DHS1 rs62059712 (T-major vs. C-minor) and ( C ) DHS2 rs74480102 (G-major vs. A-minor) in cultured primary human bronchial smooth muscle cells (HuBrSMCs). ( D ) qPCR for JMJD3 in HuSMCs with CRISPR/Cas9-mediated deletion of a 450 bp region encompassing rs62059712 within DHS1 (ΔDHS1) compared with unedited (WT) HuSMCs. ( E ) Sequence of rs62059712 T-major and C-minor sequences with SP1 consensus binding site underlined. ( F ) Western blot for SP1 after affinity purification using T versus C probes corresponding to rs62059712 SNP region incubated with human aortic smooth muscle cell (HuAoSMC) nuclear lysate. ( G ) ChIP-qPCR for Sp1 at the Jmjd3 promoter in mAoSMCs compared with IgG negative control. ( H ) ChIP-qPCR for Sp1 at the Jmjd3 promoter in mAoSMCs serum starved or treated with TGF-β. ( I ) Luciferase assays in mAoSMCs treated with siNTC and siSp1 siRNAs, then transfected with pGL3-DHS1-T and -C. ( J ) Luciferase assays in HuSMCs transfected with DHS1-C construct and then treated with TGF-β (20 ng/ml). ( K ) qPCR for Jmjd3 after NTC versus Sp1 knockdown in mAoSMCs serum starved for 16 hours. Data are represented as mean ± SEM. Results are representative of data from SMCs from 4–6 mice per group. n = 3 independent experiments. Two-tailed Student’s t test, * P < 0.05; ** P < 0.01.

    Journal: The Journal of Clinical Investigation

    Article Title: Epigenetic alteration of smooth muscle cells regulates endothelin-dependent blood pressure and hypertensive arterial remodeling

    doi: 10.1172/JCI186146

    Figure Lengend Snippet: ( A ) Schematic of the human JMJD3 gene, including gene structure, H3K27Ac, DHS, BP-associated SNPs, cloned fragments for luciferase assays, and vertebrate conservation (UCSC genome browser). ( B ) Luciferase results of DHS1 rs62059712 (T-major vs. C-minor) and ( C ) DHS2 rs74480102 (G-major vs. A-minor) in cultured primary human bronchial smooth muscle cells (HuBrSMCs). ( D ) qPCR for JMJD3 in HuSMCs with CRISPR/Cas9-mediated deletion of a 450 bp region encompassing rs62059712 within DHS1 (ΔDHS1) compared with unedited (WT) HuSMCs. ( E ) Sequence of rs62059712 T-major and C-minor sequences with SP1 consensus binding site underlined. ( F ) Western blot for SP1 after affinity purification using T versus C probes corresponding to rs62059712 SNP region incubated with human aortic smooth muscle cell (HuAoSMC) nuclear lysate. ( G ) ChIP-qPCR for Sp1 at the Jmjd3 promoter in mAoSMCs compared with IgG negative control. ( H ) ChIP-qPCR for Sp1 at the Jmjd3 promoter in mAoSMCs serum starved or treated with TGF-β. ( I ) Luciferase assays in mAoSMCs treated with siNTC and siSp1 siRNAs, then transfected with pGL3-DHS1-T and -C. ( J ) Luciferase assays in HuSMCs transfected with DHS1-C construct and then treated with TGF-β (20 ng/ml). ( K ) qPCR for Jmjd3 after NTC versus Sp1 knockdown in mAoSMCs serum starved for 16 hours. Data are represented as mean ± SEM. Results are representative of data from SMCs from 4–6 mice per group. n = 3 independent experiments. Two-tailed Student’s t test, * P < 0.05; ** P < 0.01.

    Article Snippet: DNA fragments were then cloned into pGL3 luciferase basic vector (Addgene), and the correct sequence was verified by Sanger sequencing.

    Techniques: Clone Assay, Luciferase, Cell Culture, CRISPR, Sequencing, Binding Assay, Western Blot, Affinity Purification, Incubation, ChIP-qPCR, Negative Control, Transfection, Construct, Knockdown, Two Tailed Test