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Human islets (500/condition) were treated with cytokines (100 U/ml IL-1β + 300 U/ml IFNγ) for 2-36h. In some replicates, at peak response times, cytokines + S -BEL groups were added. A. PGE 2 . B. PGF 2 α. C. 8-Iso-PGF 2 α. (Insets, *,† significantly different from DMSO group, p < 0.05 and p < 0.01, respectively, n=10-18. *CTK+ S -BEL group significantly different from CTK group, p < 0.05, n values indicated above the bars.)

Journal: bioRxiv

Article Title: ER Stress-Induced β-Cell Apoptosis is Linked to Novel Select Lipid Signaling at the Transcriptional Level: Implications in T1D Developmen t

doi: 10.64898/2026.03.02.708596

Figure Lengend Snippet: Human islets (500/condition) were treated with cytokines (100 U/ml IL-1β + 300 U/ml IFNγ) for 2-36h. In some replicates, at peak response times, cytokines + S -BEL groups were added. A. PGE 2 . B. PGF 2 α. C. 8-Iso-PGF 2 α. (Insets, *,† significantly different from DMSO group, p < 0.05 and p < 0.01, respectively, n=10-18. *CTK+ S -BEL group significantly different from CTK group, p < 0.05, n values indicated above the bars.)

Article Snippet: To assess the role of select pathways, cells were treated with 10 nM PGE 2 (#14010), 10 μM PGF 2 α (#16010), 10 μM 8-Iso PGF 2 α (#16350, Cayman Chemical, Ann Arbor, Michigan)

Techniques:

MIN-6 β-cells were treated with D MSO, C ytokines, or PGs (10 nM PGE 2 , 10 µM PGF 2 α, 10 µM 8-Iso-PGF 2 α) for 24h and processed for TUNEL. The mean ± SEMs of %apoptotic cells, relative to total cells, are presented. ( a- e Significantly different from DMSO group, p < 0.05 - p <0.001.)

Journal: bioRxiv

Article Title: ER Stress-Induced β-Cell Apoptosis is Linked to Novel Select Lipid Signaling at the Transcriptional Level: Implications in T1D Developmen t

doi: 10.64898/2026.03.02.708596

Figure Lengend Snippet: MIN-6 β-cells were treated with D MSO, C ytokines, or PGs (10 nM PGE 2 , 10 µM PGF 2 α, 10 µM 8-Iso-PGF 2 α) for 24h and processed for TUNEL. The mean ± SEMs of %apoptotic cells, relative to total cells, are presented. ( a- e Significantly different from DMSO group, p < 0.05 - p <0.001.)

Article Snippet: To assess the role of select pathways, cells were treated with 10 nM PGE 2 (#14010), 10 μM PGF 2 α (#16010), 10 μM 8-Iso PGF 2 α (#16350, Cayman Chemical, Ann Arbor, Michigan)

Techniques: TUNEL Assay

A. MIN6 β-cells were treated with D MSO, C ytokines as a positive control (50 U/ml IL-1β + 150 U/ml IFNγ) or a PGs cocktail (10 nM PGE 2 + 10 µM PGF 2 α + 10 µM 8-Iso-PGF 2 α) and processed for TUNEL analyses after 24h. Data are means ± SEMs of % apoptotic cells, relative to total cells. ( a Cytokine group significantly different from DMSO group, p < 0.0001; b PGs group significantly different from DMSO group, p = 0.004, n=3/group). B . Representative immunoblotting analyses blots for p65-NFκB, pSTAT1, pPERK, CHOP, and loading control tubulin after 16h. C-F . Corresponding cumulative densitometries are presented. ( a,b,c Cytokine or PGs group significantly different from DMSO groups, p, 0.05, p = 0.0013, and p = 0.009, respectively, n=3-4/group. ND=not determined.)

Journal: bioRxiv

Article Title: ER Stress-Induced β-Cell Apoptosis is Linked to Novel Select Lipid Signaling at the Transcriptional Level: Implications in T1D Developmen t

doi: 10.64898/2026.03.02.708596

Figure Lengend Snippet: A. MIN6 β-cells were treated with D MSO, C ytokines as a positive control (50 U/ml IL-1β + 150 U/ml IFNγ) or a PGs cocktail (10 nM PGE 2 + 10 µM PGF 2 α + 10 µM 8-Iso-PGF 2 α) and processed for TUNEL analyses after 24h. Data are means ± SEMs of % apoptotic cells, relative to total cells. ( a Cytokine group significantly different from DMSO group, p < 0.0001; b PGs group significantly different from DMSO group, p = 0.004, n=3/group). B . Representative immunoblotting analyses blots for p65-NFκB, pSTAT1, pPERK, CHOP, and loading control tubulin after 16h. C-F . Corresponding cumulative densitometries are presented. ( a,b,c Cytokine or PGs group significantly different from DMSO groups, p, 0.05, p = 0.0013, and p = 0.009, respectively, n=3-4/group. ND=not determined.)

Article Snippet: To assess the role of select pathways, cells were treated with 10 nM PGE 2 (#14010), 10 μM PGF 2 α (#16010), 10 μM 8-Iso PGF 2 α (#16350, Cayman Chemical, Ann Arbor, Michigan)

Techniques: Positive Control, TUNEL Assay, Western Blot, Control