Journal: Antioxidants

Article Title: Vasodilator Responses of Perivascular Adipose Tissue-Derived Hydrogen Sulfide Stimulated with L-Cysteine in Pregnancy Hypertension-Induced Endothelial Dysfunction in Rats

doi: 10.3390/antiox12111919

Figure Lengend Snippet: Levels of anti-angiogenic (soluble fms-like tyrosine kinase-1: sFlt-1) and angiogenic (placental growth factor: PlGF) factors in plasma. sFlt-1/PlGF ratio ( A ), plasma levels of PlGF ( B ), and sFlt-1 ( C ) in Non-Preg, Non-Preg+DOCA, Preg, and Preg+DOCA groups. Values are means ± SEM. Symbols indicate significant differences (* p < 0.05) versus Preg group.

Article Snippet: Plasma levels of anti-angiogenic factor sFlt-1 (#ELM-VEGFR1, RayBiotech Inc., Norcross, GA, USA) and angiogenic factor PLGF (#E-EL-R0742, Elabscience Inc., Houston, TX, USA) were determined according to the manufacturer’s instructions of the commercial enzyme-linked immunoassay (ELISA) kits.

Techniques: Clinical Proteomics

Journal: JHEP Reports

Article Title: Placental growth factor modulates endothelial NO production and exacerbates experimental hepatopulmonary syndrome

doi: 10.1016/j.jhepr.2024.101297

Figure Lengend Snippet: HPS development in Plgf –/– rats after CBDL and PPVL surgery. (A) Experimental procedures. (B) Circulating PlGF levels in serum determined using ELISA. (C) Detection of Plgf mRNA levels in liver and lung using RT-qPCR. (D) A-aO 2 in arterial blood. (E) Quantification of contrast-enhanced transthoracic echocardiogram: ratio of the difference in acoustic signal between baseline and after injection in the left ventricle to the right ventricle. (F) Quantification of total microspheres in one kidney by fluorescence-activated cell sorting after jugular vein injection. Data are mean ± SEM; comparisons were made using one-way ANOVA followed by the Tukey test: n.s., not significant ( p >0.05); ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001, vs. Sham WT group; &&& p <0.001, &&&& p <0.0001 vs. WT CBDL. A-aO 2 , alveolar–arterial oxygen gradient; CBDL, common bile duct ligation; HPS, hepatopulmonary syndrome; PIGF, placental growth factor; PPVL, partial portal vein ligation; real-time quantitative PCR (RT-qPCR); WT, wild-type.

Article Snippet: Serum PlGF levels were quantified using an ELISA kit (E-CL-R0520, ElabScience, Houston, Texas, 77079, USA) following the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Injection, Fluorescence, FACS, Ligation, Real-time Polymerase Chain Reaction

Journal: Diabetes Therapy

Article Title: Concentrations of VEGF and PlGF Decrease in Eyes After Intravitreal Conbercept Injection

doi: 10.1007/s13300-018-0527-9

Figure Lengend Snippet: Before and after the patients received the intravitreal conbercept treatment and underwent vitrectomy. a Schematic flow diagram of the process in this work. b Concentrations of VEGF and PlGF in the aqueous and vitreous humor pre- and post-intravitreal conbercept injection. c Correlation between the concentrations of VEGF and PlGF in the aqueous and vitreous humor. ** p < 0.01

Article Snippet: ELISA analysis was performed using Human VEGF ELISA kit and Human PlGF ELISA kit (Boster Biological Technology, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Injection