pgem-t easy a1360 (Promega)
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Pgem T Easy A1360, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-t+easy/pgem+t+easy/bio_rxiv__2025__07__16__665078-162-5-7
Average 90 stars, based on 1 article reviews
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Polymerase Chain Reaction:Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks). Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation Article Snippet: PCR products were cloned into Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into Article Title: Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent Article Title: Zebrafish sall1a and sall4 contribute to body elongation. Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos. Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori . Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus. Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing. Article Snippet: PCR products were gel-purified and ligated into Mutagenesis:Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks). Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation Article Snippet: PCR products were cloned into Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into Article Title: Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent Article Title: Zebrafish sall1a and sall4 contribute to body elongation. Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos. Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori . Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus. Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing. Article Snippet: PCR products were gel-purified and ligated into Transformation Assay:Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks). Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation Article Snippet: PCR products were cloned into Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into Article Title: Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent Article Title: Zebrafish sall1a and sall4 contribute to body elongation. Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos. Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori . Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus. Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing. Article Snippet: PCR products were gel-purified and ligated into Sequencing:Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks). Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation Article Snippet: PCR products were cloned into Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into Article Title: Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent Article Title: Zebrafish sall1a and sall4 contribute to body elongation. Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos. Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori . Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus. Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing. Article Snippet: PCR products were gel-purified and ligated into Cloning:Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks). Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation Article Snippet: PCR products were cloned into Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into Article Title: Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent Article Title: Zebrafish sall1a and sall4 contribute to body elongation. Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos. Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori . Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus. Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing. Article Snippet: PCR products were gel-purified and ligated into Clone Assay:Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks). Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation Article Snippet: PCR products were cloned into Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into Article Title: Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent Article Title: Zebrafish sall1a and sall4 contribute to body elongation. Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos. Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori . Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus. Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing. Article Snippet: PCR products were gel-purified and ligated into Plasmid Preparation:Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks). Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation Article Snippet: PCR products were cloned into Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into Article Title: Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent Article Title: Zebrafish sall1a and sall4 contribute to body elongation. Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos. Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori . Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus. Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing. Article Snippet: PCR products were gel-purified and ligated into Expressing:Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks). Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation Article Snippet: PCR products were cloned into Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into Article Title: Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent Article Title: Zebrafish sall1a and sall4 contribute to body elongation. Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos. Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori . Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus. Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing. Article Snippet: PCR products were gel-purified and ligated into Amplification:Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks). Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation Article Snippet: PCR products were cloned into Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into Article Title: Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent Article Title: Zebrafish sall1a and sall4 contribute to body elongation. Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos. Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori . Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus. Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing. Article Snippet: PCR products were gel-purified and ligated into cDNA Library Assay:Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks). Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation Article Snippet: PCR products were cloned into Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into Article Title: Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent Article Title: Zebrafish sall1a and sall4 contribute to body elongation. Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos. Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori . Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus. Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing. Article Snippet: PCR products were gel-purified and ligated into |
