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Promega pgem-t easy a1360
Pgem T Easy A1360, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-t+easy/pgem+t+easy/bio_rxiv__2025__07__16__665078-162-5-7
Average 90 stars, based on 1 article reviews
pgem-t easy a1360 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis
Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks).

Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation
Article Snippet: PCR products were cloned into pGEM-T Easy (Promega), and at least 10 clones per sample were sequenced.

Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging
Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into vector pGEM-T Easy (Promega, Madison, WI) for Sanger sequencing to identify the mutation.

Article Title:
Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent M. tuberculosis parental strain (11) Plasmids Description Reference pGEM-T easy T/A cloning vector Promega, UK pET28b Prokaryotic expression vector Novagen, UK pET28b-ppk-1(M. tuberculosis) pET28b harboring Rv2984 (1) pET28b-ppk-1(E. coli) pET28b harboring E. coli ppk-1 This study pET28b-ppk-1 (Klebsiella pneumoniae) pET28b harboring Klebsiella pneumonia ppk-1 This study pET28b-accA1 pET28b harboring Rv2501c This study pET28b-accA2 pET28b harboring Rv0973c This study pET28b-accD1 pET28b harboring Rv2502c This study pET28b-accD2 pET28b harboring Rv0974c This study pET28b-accD4 pET28b harboring Rv3799c This study pET28b-accE5 pET28b harboring Rv3281 This study Cell line Description Reference THP-1 Human monocyte cell line NCCS PUNE

Article Title: Zebrafish sall1a and sall4 contribute to body elongation.
Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos.

Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori .
Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into pGEM- T Easy (Promega) to generate plasmid pKR5 (SI Appendix, Fig. S10).

Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus.
Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the SalI site) and 5’-TCA GGG CTT TGG CCT TGT ACT GCC T-3’ and subcloned into pGEM-T easy (Promega, Madison, WI).

Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing.
Article Snippet: PCR products were gel-purified and ligated into pGEM-T Easy (Promega) for mutation of 11870, 13770, 08770, 00420, and 12705 or retained as a linear fragment for mutating 06635 and 06500.

Mutagenesis:

Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis
Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks).

Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation
Article Snippet: PCR products were cloned into pGEM-T Easy (Promega), and at least 10 clones per sample were sequenced.

Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging
Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into vector pGEM-T Easy (Promega, Madison, WI) for Sanger sequencing to identify the mutation.

Article Title:
Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent M. tuberculosis parental strain (11) Plasmids Description Reference pGEM-T easy T/A cloning vector Promega, UK pET28b Prokaryotic expression vector Novagen, UK pET28b-ppk-1(M. tuberculosis) pET28b harboring Rv2984 (1) pET28b-ppk-1(E. coli) pET28b harboring E. coli ppk-1 This study pET28b-ppk-1 (Klebsiella pneumoniae) pET28b harboring Klebsiella pneumonia ppk-1 This study pET28b-accA1 pET28b harboring Rv2501c This study pET28b-accA2 pET28b harboring Rv0973c This study pET28b-accD1 pET28b harboring Rv2502c This study pET28b-accD2 pET28b harboring Rv0974c This study pET28b-accD4 pET28b harboring Rv3799c This study pET28b-accE5 pET28b harboring Rv3281 This study Cell line Description Reference THP-1 Human monocyte cell line NCCS PUNE

Article Title: Zebrafish sall1a and sall4 contribute to body elongation.
Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos.

Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori .
Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into pGEM- T Easy (Promega) to generate plasmid pKR5 (SI Appendix, Fig. S10).

Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus.
Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the SalI site) and 5’-TCA GGG CTT TGG CCT TGT ACT GCC T-3’ and subcloned into pGEM-T easy (Promega, Madison, WI).

Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing.
Article Snippet: PCR products were gel-purified and ligated into pGEM-T Easy (Promega) for mutation of 11870, 13770, 08770, 00420, and 12705 or retained as a linear fragment for mutating 06635 and 06500.

Transformation Assay:

Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis
Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks).

Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation
Article Snippet: PCR products were cloned into pGEM-T Easy (Promega), and at least 10 clones per sample were sequenced.

Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging
Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into vector pGEM-T Easy (Promega, Madison, WI) for Sanger sequencing to identify the mutation.

Article Title:
Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent M. tuberculosis parental strain (11) Plasmids Description Reference pGEM-T easy T/A cloning vector Promega, UK pET28b Prokaryotic expression vector Novagen, UK pET28b-ppk-1(M. tuberculosis) pET28b harboring Rv2984 (1) pET28b-ppk-1(E. coli) pET28b harboring E. coli ppk-1 This study pET28b-ppk-1 (Klebsiella pneumoniae) pET28b harboring Klebsiella pneumonia ppk-1 This study pET28b-accA1 pET28b harboring Rv2501c This study pET28b-accA2 pET28b harboring Rv0973c This study pET28b-accD1 pET28b harboring Rv2502c This study pET28b-accD2 pET28b harboring Rv0974c This study pET28b-accD4 pET28b harboring Rv3799c This study pET28b-accE5 pET28b harboring Rv3281 This study Cell line Description Reference THP-1 Human monocyte cell line NCCS PUNE

Article Title: Zebrafish sall1a and sall4 contribute to body elongation.
Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos.

Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori .
Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into pGEM- T Easy (Promega) to generate plasmid pKR5 (SI Appendix, Fig. S10).

Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus.
Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the SalI site) and 5’-TCA GGG CTT TGG CCT TGT ACT GCC T-3’ and subcloned into pGEM-T easy (Promega, Madison, WI).

Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing.
Article Snippet: PCR products were gel-purified and ligated into pGEM-T Easy (Promega) for mutation of 11870, 13770, 08770, 00420, and 12705 or retained as a linear fragment for mutating 06635 and 06500.

Sequencing:

Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis
Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks).

Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation
Article Snippet: PCR products were cloned into pGEM-T Easy (Promega), and at least 10 clones per sample were sequenced.

Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging
Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into vector pGEM-T Easy (Promega, Madison, WI) for Sanger sequencing to identify the mutation.

Article Title:
Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent M. tuberculosis parental strain (11) Plasmids Description Reference pGEM-T easy T/A cloning vector Promega, UK pET28b Prokaryotic expression vector Novagen, UK pET28b-ppk-1(M. tuberculosis) pET28b harboring Rv2984 (1) pET28b-ppk-1(E. coli) pET28b harboring E. coli ppk-1 This study pET28b-ppk-1 (Klebsiella pneumoniae) pET28b harboring Klebsiella pneumonia ppk-1 This study pET28b-accA1 pET28b harboring Rv2501c This study pET28b-accA2 pET28b harboring Rv0973c This study pET28b-accD1 pET28b harboring Rv2502c This study pET28b-accD2 pET28b harboring Rv0974c This study pET28b-accD4 pET28b harboring Rv3799c This study pET28b-accE5 pET28b harboring Rv3281 This study Cell line Description Reference THP-1 Human monocyte cell line NCCS PUNE

Article Title: Zebrafish sall1a and sall4 contribute to body elongation.
Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos.

Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori .
Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into pGEM- T Easy (Promega) to generate plasmid pKR5 (SI Appendix, Fig. S10).

Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus.
Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the SalI site) and 5’-TCA GGG CTT TGG CCT TGT ACT GCC T-3’ and subcloned into pGEM-T easy (Promega, Madison, WI).

Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing.
Article Snippet: PCR products were gel-purified and ligated into pGEM-T Easy (Promega) for mutation of 11870, 13770, 08770, 00420, and 12705 or retained as a linear fragment for mutating 06635 and 06500.

Cloning:

Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis
Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks).

Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation
Article Snippet: PCR products were cloned into pGEM-T Easy (Promega), and at least 10 clones per sample were sequenced.

Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging
Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into vector pGEM-T Easy (Promega, Madison, WI) for Sanger sequencing to identify the mutation.

Article Title:
Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent M. tuberculosis parental strain (11) Plasmids Description Reference pGEM-T easy T/A cloning vector Promega, UK pET28b Prokaryotic expression vector Novagen, UK pET28b-ppk-1(M. tuberculosis) pET28b harboring Rv2984 (1) pET28b-ppk-1(E. coli) pET28b harboring E. coli ppk-1 This study pET28b-ppk-1 (Klebsiella pneumoniae) pET28b harboring Klebsiella pneumonia ppk-1 This study pET28b-accA1 pET28b harboring Rv2501c This study pET28b-accA2 pET28b harboring Rv0973c This study pET28b-accD1 pET28b harboring Rv2502c This study pET28b-accD2 pET28b harboring Rv0974c This study pET28b-accD4 pET28b harboring Rv3799c This study pET28b-accE5 pET28b harboring Rv3281 This study Cell line Description Reference THP-1 Human monocyte cell line NCCS PUNE

Article Title: Zebrafish sall1a and sall4 contribute to body elongation.
Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos.

Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori .
Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into pGEM- T Easy (Promega) to generate plasmid pKR5 (SI Appendix, Fig. S10).

Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus.
Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the SalI site) and 5’-TCA GGG CTT TGG CCT TGT ACT GCC T-3’ and subcloned into pGEM-T easy (Promega, Madison, WI).

Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing.
Article Snippet: PCR products were gel-purified and ligated into pGEM-T Easy (Promega) for mutation of 11870, 13770, 08770, 00420, and 12705 or retained as a linear fragment for mutating 06635 and 06500.

Clone Assay:

Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis
Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks).

Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation
Article Snippet: PCR products were cloned into pGEM-T Easy (Promega), and at least 10 clones per sample were sequenced.

Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging
Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into vector pGEM-T Easy (Promega, Madison, WI) for Sanger sequencing to identify the mutation.

Article Title:
Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent M. tuberculosis parental strain (11) Plasmids Description Reference pGEM-T easy T/A cloning vector Promega, UK pET28b Prokaryotic expression vector Novagen, UK pET28b-ppk-1(M. tuberculosis) pET28b harboring Rv2984 (1) pET28b-ppk-1(E. coli) pET28b harboring E. coli ppk-1 This study pET28b-ppk-1 (Klebsiella pneumoniae) pET28b harboring Klebsiella pneumonia ppk-1 This study pET28b-accA1 pET28b harboring Rv2501c This study pET28b-accA2 pET28b harboring Rv0973c This study pET28b-accD1 pET28b harboring Rv2502c This study pET28b-accD2 pET28b harboring Rv0974c This study pET28b-accD4 pET28b harboring Rv3799c This study pET28b-accE5 pET28b harboring Rv3281 This study Cell line Description Reference THP-1 Human monocyte cell line NCCS PUNE

Article Title: Zebrafish sall1a and sall4 contribute to body elongation.
Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos.

Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori .
Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into pGEM- T Easy (Promega) to generate plasmid pKR5 (SI Appendix, Fig. S10).

Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus.
Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the SalI site) and 5’-TCA GGG CTT TGG CCT TGT ACT GCC T-3’ and subcloned into pGEM-T easy (Promega, Madison, WI).

Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing.
Article Snippet: PCR products were gel-purified and ligated into pGEM-T Easy (Promega) for mutation of 11870, 13770, 08770, 00420, and 12705 or retained as a linear fragment for mutating 06635 and 06500.

Plasmid Preparation:

Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis
Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks).

Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation
Article Snippet: PCR products were cloned into pGEM-T Easy (Promega), and at least 10 clones per sample were sequenced.

Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging
Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into vector pGEM-T Easy (Promega, Madison, WI) for Sanger sequencing to identify the mutation.

Article Title:
Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent M. tuberculosis parental strain (11) Plasmids Description Reference pGEM-T easy T/A cloning vector Promega, UK pET28b Prokaryotic expression vector Novagen, UK pET28b-ppk-1(M. tuberculosis) pET28b harboring Rv2984 (1) pET28b-ppk-1(E. coli) pET28b harboring E. coli ppk-1 This study pET28b-ppk-1 (Klebsiella pneumoniae) pET28b harboring Klebsiella pneumonia ppk-1 This study pET28b-accA1 pET28b harboring Rv2501c This study pET28b-accA2 pET28b harboring Rv0973c This study pET28b-accD1 pET28b harboring Rv2502c This study pET28b-accD2 pET28b harboring Rv0974c This study pET28b-accD4 pET28b harboring Rv3799c This study pET28b-accE5 pET28b harboring Rv3281 This study Cell line Description Reference THP-1 Human monocyte cell line NCCS PUNE

Article Title: Zebrafish sall1a and sall4 contribute to body elongation.
Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos.

Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori .
Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into pGEM- T Easy (Promega) to generate plasmid pKR5 (SI Appendix, Fig. S10).

Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus.
Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the SalI site) and 5’-TCA GGG CTT TGG CCT TGT ACT GCC T-3’ and subcloned into pGEM-T easy (Promega, Madison, WI).

Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing.
Article Snippet: PCR products were gel-purified and ligated into pGEM-T Easy (Promega) for mutation of 11870, 13770, 08770, 00420, and 12705 or retained as a linear fragment for mutating 06635 and 06500.

Expressing:

Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis
Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks).

Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation
Article Snippet: PCR products were cloned into pGEM-T Easy (Promega), and at least 10 clones per sample were sequenced.

Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging
Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into vector pGEM-T Easy (Promega, Madison, WI) for Sanger sequencing to identify the mutation.

Article Title:
Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent M. tuberculosis parental strain (11) Plasmids Description Reference pGEM-T easy T/A cloning vector Promega, UK pET28b Prokaryotic expression vector Novagen, UK pET28b-ppk-1(M. tuberculosis) pET28b harboring Rv2984 (1) pET28b-ppk-1(E. coli) pET28b harboring E. coli ppk-1 This study pET28b-ppk-1 (Klebsiella pneumoniae) pET28b harboring Klebsiella pneumonia ppk-1 This study pET28b-accA1 pET28b harboring Rv2501c This study pET28b-accA2 pET28b harboring Rv0973c This study pET28b-accD1 pET28b harboring Rv2502c This study pET28b-accD2 pET28b harboring Rv0974c This study pET28b-accD4 pET28b harboring Rv3799c This study pET28b-accE5 pET28b harboring Rv3281 This study Cell line Description Reference THP-1 Human monocyte cell line NCCS PUNE

Article Title: Zebrafish sall1a and sall4 contribute to body elongation.
Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos.

Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori .
Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into pGEM- T Easy (Promega) to generate plasmid pKR5 (SI Appendix, Fig. S10).

Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus.
Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the SalI site) and 5’-TCA GGG CTT TGG CCT TGT ACT GCC T-3’ and subcloned into pGEM-T easy (Promega, Madison, WI).

Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing.
Article Snippet: PCR products were gel-purified and ligated into pGEM-T Easy (Promega) for mutation of 11870, 13770, 08770, 00420, and 12705 or retained as a linear fragment for mutating 06635 and 06500.

Amplification:

Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis
Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks).

Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation
Article Snippet: PCR products were cloned into pGEM-T Easy (Promega), and at least 10 clones per sample were sequenced.

Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging
Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into vector pGEM-T Easy (Promega, Madison, WI) for Sanger sequencing to identify the mutation.

Article Title:
Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent M. tuberculosis parental strain (11) Plasmids Description Reference pGEM-T easy T/A cloning vector Promega, UK pET28b Prokaryotic expression vector Novagen, UK pET28b-ppk-1(M. tuberculosis) pET28b harboring Rv2984 (1) pET28b-ppk-1(E. coli) pET28b harboring E. coli ppk-1 This study pET28b-ppk-1 (Klebsiella pneumoniae) pET28b harboring Klebsiella pneumonia ppk-1 This study pET28b-accA1 pET28b harboring Rv2501c This study pET28b-accA2 pET28b harboring Rv0973c This study pET28b-accD1 pET28b harboring Rv2502c This study pET28b-accD2 pET28b harboring Rv0974c This study pET28b-accD4 pET28b harboring Rv3799c This study pET28b-accE5 pET28b harboring Rv3281 This study Cell line Description Reference THP-1 Human monocyte cell line NCCS PUNE

Article Title: Zebrafish sall1a and sall4 contribute to body elongation.
Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos.

Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori .
Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into pGEM- T Easy (Promega) to generate plasmid pKR5 (SI Appendix, Fig. S10).

Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus.
Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the SalI site) and 5’-TCA GGG CTT TGG CCT TGT ACT GCC T-3’ and subcloned into pGEM-T easy (Promega, Madison, WI).

Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing.
Article Snippet: PCR products were gel-purified and ligated into pGEM-T Easy (Promega) for mutation of 11870, 13770, 08770, 00420, and 12705 or retained as a linear fragment for mutating 06635 and 06500.

cDNA Library Assay:

Article Title: Mfge8 Knockout Affects Tail Resorption Speed During Xenopus Metamorphosis
Article Snippet: The BioOne Digital Library (https://bioone.org/) provides worldwide distribution for more than 580 journals and eBooks from BioOne’s community of over 150 nonprofit societies, research institutions, and university presses in the biological, ecological, and environmental sciences.. The BioOne Digital Library encompasses the flagship aggregation BioOne Complete (https://bioone.org/subscribe), the BioOne Complete Archive (https://bioone.org/archive), and the BioOne eBooks program offerings ESA eBook Collection (https://bioone.org/esa-ebooks) and CSIRO Publishing BioSelect Collection (https://bioone.org/csiroebooks).

Article Title: Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation
Article Snippet: PCR products were cloned into pGEM-T Easy (Promega), and at least 10 clones per sample were sequenced.

Article Title: The neuropeptide sulfakinin is a peripheral regulator of insect behavioral switch between mating and foraging
Article Snippet: The fragments were genotyped using the QIAxcel Advanced System (QIAGEN, Hilden, Germany), and potential edited products were directly sequenced or cloned into vector pGEM-T Easy (Promega, Madison, WI) for Sanger sequencing to identify the mutation.

Article Title:
Article Snippet: Bacterial strains Description Reference E. coli XL-1 blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F ́ proAB lacIq Z∆M15 Tn10 (Tetr)] Stratagene, USA E. coli MG1655 K-12 F- lambda- ilvG- rfb50 rph-1 E. coli Genetic Stock Center (CGSC 6300) E. coli BL-21 pGro7 CmR, ParaB::groES-groEL encoding GroES/EL Takara Bio E. coli BL-21 Rosetta F− ompT hsdSB(rB −mB −) gal dcm (DE3) pRARE Novagen M. tuberculosis H37Rv M. tuberculosis parental strain ATCC Δppk-1 Rv2984 mutant strain of M. tuberculosis (1) Δppk-1-CT Rv2984 mutant strain of M. tuberculosis complemented with Rv1026 (1) dkppx Double mutant strain of M. tuberculosis with deletion of Rv0496 and Rv1026. (37) M. tuberculosis H37Rv-lux Auto-luminescent M. tuberculosis parental strain (11) Plasmids Description Reference pGEM-T easy T/A cloning vector Promega, UK pET28b Prokaryotic expression vector Novagen, UK pET28b-ppk-1(M. tuberculosis) pET28b harboring Rv2984 (1) pET28b-ppk-1(E. coli) pET28b harboring E. coli ppk-1 This study pET28b-ppk-1 (Klebsiella pneumoniae) pET28b harboring Klebsiella pneumonia ppk-1 This study pET28b-accA1 pET28b harboring Rv2501c This study pET28b-accA2 pET28b harboring Rv0973c This study pET28b-accD1 pET28b harboring Rv2502c This study pET28b-accD2 pET28b harboring Rv0974c This study pET28b-accD4 pET28b harboring Rv3799c This study pET28b-accE5 pET28b harboring Rv3281 This study Cell line Description Reference THP-1 Human monocyte cell line NCCS PUNE

Article Title: Zebrafish sall1a and sall4 contribute to body elongation.
Article Snippet: Vertebrate embryos elongate the body after gastrulation.. Unlike in mice, which elongate both the trunk and tail after gastrulation, zebrafish embryos complete development of the trunk by the end of gastrulation, followed by tail elongation by proliferation of posteriorly located progenitors.. Previous studies have shown that the transcription factors SALL1 and SALL4 redundantly regulate tail elongation in mouse embryos.

Article Title: FlgY, PflA, and PflB form a spoke-ring network in the high-torque flagellar motor of Helicobacter pylori .
Article Snippet: The amplicons upstream and downstream of pflA were joined via overlapping PCR, treated with Taq polymerase to add A overhangs to their 3’- ends, and ligated into pGEM- T Easy (Promega) to generate plasmid pKR5 (SI Appendix, Fig. S10).

Article Title: EFA6A, a Guanine Nucleotide Exchange Factor for Arf6, Regulates Developmental Stage-Dependent Spine Morphogenesis, Synaptic Plasticity, and Long-Term Memory in the Hippocampus.
Article Snippet: For a mammalian expression vector encoding EFA6As, the cDNA fragment encoding the full length of EFA6As was amplified from mouse brain cDNA library by PCR with primers 5’-GTC GAC CAT GAT CGG CGT CAA CAG CAT CCA C-3’ (the underlined region indicates the SalI site) and 5’-TCA GGG CTT TGG CCT TGT ACT GCC T-3’ and subcloned into pGEM-T easy (Promega, Madison, WI).

Article Title: Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin- and serum-mediated killing.
Article Snippet: PCR products were gel-purified and ligated into pGEM-T Easy (Promega) for mutation of 11870, 13770, 08770, 00420, and 12705 or retained as a linear fragment for mutating 06635 and 06500.



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pTYAa01, designed to generate a cdtABC knockout with a kanamycin-resistance cassette via homologous recombination. (B) pTYAa02, designed to generate a cdtABC knockout with a spectinomycin-resistance cassette. (C-E) pTYAa03 ( cdtA -His 6 ), pTYAa04 ( cdtB -His 6 ), and pTYAa05 ( cdtC -His 6 ), cloned with 5’UTRs including the native promoter region of the cdtABC operon. (F) pTYAa06, designed to generate a cdtABC complement with a spectinomycin-resistance cassette via homologous recombination.

Journal: bioRxiv

Article Title: Periodontal pathogen-derived extracellular vesicles promote EGFR-dependent malignant traits in human pancreatic cancer cells

doi: 10.64898/2026.01.29.700732

Figure Lengend Snippet: pTYAa01, designed to generate a cdtABC knockout with a kanamycin-resistance cassette via homologous recombination. (B) pTYAa02, designed to generate a cdtABC knockout with a spectinomycin-resistance cassette. (C-E) pTYAa03 ( cdtA -His 6 ), pTYAa04 ( cdtB -His 6 ), and pTYAa05 ( cdtC -His 6 ), cloned with 5’UTRs including the native promoter region of the cdtABC operon. (F) pTYAa06, designed to generate a cdtABC complement with a spectinomycin-resistance cassette via homologous recombination.

Article Snippet: Approximately 1-kb fragments flanking the km cassette and the spectinomycin-resistance cassette (amplified from pUTmini-Tn5 Sm) were assembled into NdeI-digested pGEM-T Easy (pTYAa02; ) using NEBuilder.

Techniques: Knock-Out, Homologous Recombination, Clone Assay