pgc1α  (Proteintech)

Journal: Cell Reports Medicine

Article Title: Enalaprilat reverses neutrophil polarization imbalance via targeting taurine-STING axis for treatment of diabetic wounds

doi: 10.1016/j.xcrm.2026.102714

Figure Lengend Snippet: Inhibitory effects of Ena on HUVEC ferroptosis activated by AGE-elicited neutrophils (A) CCK-8 assay was performed to measure the viability of HUVECs. n = 3 independent experiments. (B) Death rate of HUVECs incubated with neutrophils pretreated by different strategies as detected using flow cytometry. n = 3 independent experiments. (C) Levels of MDA and GSH were quantified to assess the ferroptosis activity. n = 3 independent experiments. (D) Intracellular lipid peroxidation was visualized by fluorescence staining. Scale bar, 10 μm; n = 3 independent experiments. (E) Fe 2+ ion content was visualized by fluorescence staining. Scale bar, 10 μm; n = 3 independent experiments. (F) JC-1 kit was used to evaluate the mitochondrial membrane potential of HUVECs. Scale bar, 10 μm; n = 3 independent experiments. (G) DCFH-DA fluorescence probe was applied to determine ROS abundance in HUVECs. Scale bar, 200 μm; n = 3 independent experiments. (H) Mitochondrial morphology in HUVECs as analyzed by transmission electron microscopy. Scale bar, 500 nm; n = 3 independent experiments. (I) Western blot detection of PGC1α and KLF9 in HUVECs with different treatments. n = 3 independent experiments. (J) Expression of KLF9 in HUVECs incubated with neutrophils pretreated by different approaches as detected using immunofluorescence staining. Scale bar, 10 μm; n = 3 independent experiments. (K) Expression of PGC1α in HUVECs incubated with neutrophils pretreated by different approaches as detected using immunofluorescence staining. Scale bar, 10 μm; n = 3 independent experiments. (L) Schematic diagram of Ena-elicited alleviation on endothelial cell ferroptosis induced by pro-inflammatory neutrophils. Data were shown as mean ± standard deviation (SD) from biological replicates, and statistical comparisons were performed using one-way ANOVA followed by Tukey’s multiple comparisons test in (A–G and I–K). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Expression vectors encoding ZNF460, GGT1, KLF9 and PGC1α were constructed by GeneChem (Shanghai, China) via cloning the open reading frames of the indicated genes into a pcDNA3.1 plasmid vector.

Techniques: CCK-8 Assay, Incubation, Flow Cytometry, Activity Assay, Fluorescence, Staining, Membrane, Transmission Assay, Electron Microscopy, Western Blot, Expressing, Immunofluorescence, Standard Deviation

Journal: Renal Failure

Article Title: Salvianolic acid C alleviates acute kidney injury by restoring fructose-1,6-bisphosphatase 1-mediated gluconeogenesis

doi: 10.1080/0886022X.2026.2629902

Figure Lengend Snippet: SAC promotes PGC1α/FBP1 axis in AKI mouse kidneys. (A) Representative images of multiplex immunofluorescence staining in renal tissues showing DAPI (blue, nuclei), LRP2 (green, proximal tubules), and FBP1 (red) (original magnification ×40; Scale bar: 50 μm). (B) Quantitative assessment of FBP1-positive area. (C) Representative photomicrographs of FOXO1 staining in renal tissues (original magnification ×40; Scale bar: 50 μm). (D) Quantitative assessment of FOXO1-positive cells. (E) Representative photomicrographs of PGC1α staining in renal tissues (original magnification ×40; Scale bar: 50 μm). (F) Quantitative assessment of PGC1α-positive Area. NS represents not significant. * p < 0.05. ** p < 0.01. *** p < 0.001.

Article Snippet: Primary antibodies used were Cleaved-caspase-3 (Cat# 9661, Cell Signaling Technology, Danvers, MA, USA), FOXO1 (Cat# GB12286, Servicebio, Wuhan, China), and PGC1α (Cat# 66369-1-IG, Servicebio, Wuhan, Hubei, China).

Techniques: Multiplex Assay, Immunofluorescence, Staining