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chef-drii pfge apparatus  (Bio-Rad)


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    Bio-Rad chef-drii pfge apparatus
    Chef Drii Pfge Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfge+apparatus/chef+drii+pfge+apparatus/pmc11077071-94-5-8
    Average 90 stars, based on 1 article reviews
    chef-drii pfge apparatus - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Functional Analysis of a Multicopy Host-Selective ACT-Toxin Biosynthesis Gene in the Tangerine Pathotype of Alternaria alternata Using RNA Silencing
    Article Snippet: .. Running conditions (duration, voltage, linear gradient of switching time) in the PFGE apparatus (CHEF-DRII, BioRad, Hercules, CA, U.S.A.) consisted of 0.5× tris-borate-EDTA (Sambrook et al. 1989) at 8°C in 0.8% (wt/vol) agarose gel (Seakem Gold agarose; Cambrex BioScience Rockland, Inc., Rockland, ME, U.S.A.), 13:5.4:120 h/V cm–1/s, 13:5.4: 180 h/V cm–1/s. ..

    Article Title: A Polyketide Synthase Gene, ACRTS2, Is Responsible for Biosynthesis of Host-Selective ACR-Toxin in the Rough Lemon Pathotype of Alternaria alternata
    Article Snippet: .. Running conditions (duration, voltage, and linear gradient of switching time) in the PFGE apparatus (CHEF-DRII; BioRad, Richmond, CA, U.S.A.) consisted of 0.5× Tris-borate-EDTA at 8°C in 0.8% (wt/vol) agarose gel (Seakem Gold agarose; Cambrex BioScience Rockland, Rockland, ME, U.S.A.) as follows: 25 h, 1.6 V/cm, 500 s; 30 h, 1.6 V/cm, 1,000 s; and 50 h, 1.6 V/cm, 1,500 s; 32 h, 1.6 V/cm, 2,000 s. Chromosomal DNA bands were visualized by ethidium bromide staining. .. For transfer to membranes, the gel was soaked in 0.2 N HCl for 20 min to nick chromosomal DNA, then in 0.4 N NaOH plus 1.5 M NaCl for 1 h to transfer to Hybond-N+ membranes (Amersham Biosciences) by alkaline capillary transfer with 0.4 N NaOH and 1.5 M NaCl for 48 h. The membrane was baked at 80°C for 2 h to fix the DNA and hybridized at 68°C overnight with ACRTS2 probes.

    Article Title: The vertical distribution and diversity of marine bacteriophage at a station off Southern California.
    Article Snippet: Sixty-two bacteriophages were isolated on eight indigenous bacteria from a Pacific Ocean station spanning 887-m vertical depth, on two occasions between 1999 and 2000.. On the basis of 16S rRNA sequences, six hosts were tentatively identified to be in the genus Vibrio and the other two were closely related to Altermonas macleodii (W9a) and Pseudoalteromonas spp. (W13a).. Restriction fragment length polymorphism (RFLP) analysis of phage genomes using AccI and HapI showed that 16 phages infecting host C4a (Vibrio) displayed 14 unique RFLP patterns.

    Staining:

    Article Title: A Polyketide Synthase Gene, ACRTS2, Is Responsible for Biosynthesis of Host-Selective ACR-Toxin in the Rough Lemon Pathotype of Alternaria alternata
    Article Snippet: .. Running conditions (duration, voltage, and linear gradient of switching time) in the PFGE apparatus (CHEF-DRII; BioRad, Richmond, CA, U.S.A.) consisted of 0.5× Tris-borate-EDTA at 8°C in 0.8% (wt/vol) agarose gel (Seakem Gold agarose; Cambrex BioScience Rockland, Rockland, ME, U.S.A.) as follows: 25 h, 1.6 V/cm, 500 s; 30 h, 1.6 V/cm, 1,000 s; and 50 h, 1.6 V/cm, 1,500 s; 32 h, 1.6 V/cm, 2,000 s. Chromosomal DNA bands were visualized by ethidium bromide staining. .. For transfer to membranes, the gel was soaked in 0.2 N HCl for 20 min to nick chromosomal DNA, then in 0.4 N NaOH plus 1.5 M NaCl for 1 h to transfer to Hybond-N+ membranes (Amersham Biosciences) by alkaline capillary transfer with 0.4 N NaOH and 1.5 M NaCl for 48 h. The membrane was baked at 80°C for 2 h to fix the DNA and hybridized at 68°C overnight with ACRTS2 probes.

    Electrophoresis:

    Article Title: Rad51-mediated replication fork reversal is a global response to genotoxic treatments in human cells
    Article Snippet: .. Electrophoresis was performed for 21 h at 14°C in 0.9% (wt/vol) Pulse Field Certified Agarose (Bio-Rad Laboratories) containing Tris-borate/EDTA buffer in a PFGE apparatus (CHEF DR III; Bio-Rad Laboratories), according to the following protocol (block I: 9 h, 120° included angle, 5.5 V/cm, 30 to 18-s switch; block II: 6 h, 117° included angle, 4.5 V/cm, 18 to 9-s switch; block III: 6 h, 112° included angle, 4.0 V/cm, 9 to 5-s switch). .. The gel was then stained with ethidium bromide and analyzed by the AlphaImager system (ProteinSimple).

    Blocking Assay:

    Article Title: Rad51-mediated replication fork reversal is a global response to genotoxic treatments in human cells
    Article Snippet: .. Electrophoresis was performed for 21 h at 14°C in 0.9% (wt/vol) Pulse Field Certified Agarose (Bio-Rad Laboratories) containing Tris-borate/EDTA buffer in a PFGE apparatus (CHEF DR III; Bio-Rad Laboratories), according to the following protocol (block I: 9 h, 120° included angle, 5.5 V/cm, 30 to 18-s switch; block II: 6 h, 117° included angle, 4.5 V/cm, 18 to 9-s switch; block III: 6 h, 112° included angle, 4.0 V/cm, 9 to 5-s switch). .. The gel was then stained with ethidium bromide and analyzed by the AlphaImager system (ProteinSimple).

    Pulsed-Field Gel:

    Article Title: Recovery and potential utility of YACs as circular YACs/BACs
    Article Snippet: .. The assay conditions, the GenBank accession numbers and the primer pair sequences can be obtained from http://www.ibc.wustl.edu/cgmdata/public/stsseq.html Pulsed-field gel electrophoresis (PFGE) Total yeast DNA was prepared in agarose plugs as described ( 17 ) and then subjected to PFGE in a Bio-Rad PFGE apparatus for 20 h at 14°C with switching beginning at 20/30 s and ramping to 40/60 s. The DNA was transferred to nylon membranes and Southern blot hybridizations were carried out with [ 32 P]dCTP-labeled total human DNA or DNA fragment coding for chloramphenicol acetyl transferase gene (Amersham random priming kit, Piscataway, NJ) ( 18 ). .. Hybridized membranes were washed, exposed to Kodak XOmat AR film and developed.

    Southern Blot:

    Article Title: Recovery and potential utility of YACs as circular YACs/BACs
    Article Snippet: .. The assay conditions, the GenBank accession numbers and the primer pair sequences can be obtained from http://www.ibc.wustl.edu/cgmdata/public/stsseq.html Pulsed-field gel electrophoresis (PFGE) Total yeast DNA was prepared in agarose plugs as described ( 17 ) and then subjected to PFGE in a Bio-Rad PFGE apparatus for 20 h at 14°C with switching beginning at 20/30 s and ramping to 40/60 s. The DNA was transferred to nylon membranes and Southern blot hybridizations were carried out with [ 32 P]dCTP-labeled total human DNA or DNA fragment coding for chloramphenicol acetyl transferase gene (Amersham random priming kit, Piscataway, NJ) ( 18 ). .. Hybridized membranes were washed, exposed to Kodak XOmat AR film and developed.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Recovery and potential utility of YACs as circular YACs/BACs
    Article Snippet: .. The assay conditions, the GenBank accession numbers and the primer pair sequences can be obtained from http://www.ibc.wustl.edu/cgmdata/public/stsseq.html Pulsed-field gel electrophoresis (PFGE) Total yeast DNA was prepared in agarose plugs as described ( 17 ) and then subjected to PFGE in a Bio-Rad PFGE apparatus for 20 h at 14°C with switching beginning at 20/30 s and ramping to 40/60 s. The DNA was transferred to nylon membranes and Southern blot hybridizations were carried out with [ 32 P]dCTP-labeled total human DNA or DNA fragment coding for chloramphenicol acetyl transferase gene (Amersham random priming kit, Piscataway, NJ) ( 18 ). .. Hybridized membranes were washed, exposed to Kodak XOmat AR film and developed.



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