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pet22b backbone  (Addgene inc)


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    Structured Review

    Addgene inc pet22b backbone
    Pet22b Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet22b+backbone/PIK3CA-WT+(Plasmid+%2316643)/pmc12286862-331-8-9
    Average 91 stars, based on 26 article reviews
    pet22b backbone - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Amplification:

    Article Title: Probing condensate microenvironments with a micropeptide killswitch.
    Article Snippet: pRK5-mEGFP-NPM1-TDP43-HP plasmid was constructed by amplifying the NPM1 sequence from Addgene plasmid 131818 and cloned using the NEBuilder HiFi DNA assembly into a backbone of the pRK5-meGFP (Addgene, 18696)63 linearized by restriction digest with BsrGI and AccI (NEB, R0161). .. The amplified gene fragments were cloned into a pET22b-backbone (Addgene, 166439) linearized by restriction digest with PmlI and BsrGI, using the NEBuilder HiFi DNA assembly master mix. ..

    Article Title: Probing condensate microenvironments with a micropeptide killswitch
    Article Snippet: The NPM1 sequence was amplified from pHcRed-NPM1wt-C1 plasmid (Addgene, 131818). .. The amplified gene fragments were cloned into a pET22b-backbone (Addgene, 166439) linearized by restriction digest with PmlI and BsrGI, using the NEBuilder HiFi DNA assembly master mix. ..

    Clone Assay:

    Article Title: Probing condensate microenvironments with a micropeptide killswitch.
    Article Snippet: pRK5-mEGFP-NPM1-TDP43-HP plasmid was constructed by amplifying the NPM1 sequence from Addgene plasmid 131818 and cloned using the NEBuilder HiFi DNA assembly into a backbone of the pRK5-meGFP (Addgene, 18696)63 linearized by restriction digest with BsrGI and AccI (NEB, R0161). .. The amplified gene fragments were cloned into a pET22b-backbone (Addgene, 166439) linearized by restriction digest with PmlI and BsrGI, using the NEBuilder HiFi DNA assembly master mix. ..

    Article Title: A combinatorially complete epistatic fitness landscape in an enzyme active site
    Article Snippet: .. For the pooled-culture enrichment assays, Tm 9D8* was cloned into the arabinose-inducible pBAD24 backbone while for in vitro assays and protein expression for purification, the pET22b(+) backbone was used. pBAD24-sfGFPx1 was a gift from Sankar Adhya & Francisco Malagon (Addgene plasmid # 51558; http://n2t.net/addgene:51558 ; RRID:Addgene_51558) ( ). ..

    Article Title: A combinatorially complete epistatic fitness landscape in an enzyme active site.
    Article Snippet: .. For the pooledculture enrichment assays, Tm9D8* was cloned into the arabinose- inducible pBAD24 backbone while for in vitro assays and protein expression for purification, the pET22b(+) backbone was used. pBAD24- sfGFPx1 was a gift from Sankar Adhya & Francisco Malagon (Addgene plasmid # 51558; http://n2t.net/ addgene:51558; RRID:Addgene_51558) (53). ..

    Article Title: Probing condensate microenvironments with a micropeptide killswitch
    Article Snippet: The NPM1 sequence was amplified from pHcRed-NPM1wt-C1 plasmid (Addgene, 131818). .. The amplified gene fragments were cloned into a pET22b-backbone (Addgene, 166439) linearized by restriction digest with PmlI and BsrGI, using the NEBuilder HiFi DNA assembly master mix. ..

    In Vitro:

    Article Title: A combinatorially complete epistatic fitness landscape in an enzyme active site
    Article Snippet: .. For the pooled-culture enrichment assays, Tm 9D8* was cloned into the arabinose-inducible pBAD24 backbone while for in vitro assays and protein expression for purification, the pET22b(+) backbone was used. pBAD24-sfGFPx1 was a gift from Sankar Adhya & Francisco Malagon (Addgene plasmid # 51558; http://n2t.net/addgene:51558 ; RRID:Addgene_51558) ( ). ..

    Article Title: A combinatorially complete epistatic fitness landscape in an enzyme active site.
    Article Snippet: .. For the pooledculture enrichment assays, Tm9D8* was cloned into the arabinose- inducible pBAD24 backbone while for in vitro assays and protein expression for purification, the pET22b(+) backbone was used. pBAD24- sfGFPx1 was a gift from Sankar Adhya & Francisco Malagon (Addgene plasmid # 51558; http://n2t.net/ addgene:51558; RRID:Addgene_51558) (53). ..

    Expressing:

    Article Title: A combinatorially complete epistatic fitness landscape in an enzyme active site
    Article Snippet: .. For the pooled-culture enrichment assays, Tm 9D8* was cloned into the arabinose-inducible pBAD24 backbone while for in vitro assays and protein expression for purification, the pET22b(+) backbone was used. pBAD24-sfGFPx1 was a gift from Sankar Adhya & Francisco Malagon (Addgene plasmid # 51558; http://n2t.net/addgene:51558 ; RRID:Addgene_51558) ( ). ..

    Article Title: A combinatorially complete epistatic fitness landscape in an enzyme active site.
    Article Snippet: .. For the pooledculture enrichment assays, Tm9D8* was cloned into the arabinose- inducible pBAD24 backbone while for in vitro assays and protein expression for purification, the pET22b(+) backbone was used. pBAD24- sfGFPx1 was a gift from Sankar Adhya & Francisco Malagon (Addgene plasmid # 51558; http://n2t.net/ addgene:51558; RRID:Addgene_51558) (53). ..

    Purification:

    Article Title: A combinatorially complete epistatic fitness landscape in an enzyme active site
    Article Snippet: .. For the pooled-culture enrichment assays, Tm 9D8* was cloned into the arabinose-inducible pBAD24 backbone while for in vitro assays and protein expression for purification, the pET22b(+) backbone was used. pBAD24-sfGFPx1 was a gift from Sankar Adhya & Francisco Malagon (Addgene plasmid # 51558; http://n2t.net/addgene:51558 ; RRID:Addgene_51558) ( ). ..

    Article Title: A combinatorially complete epistatic fitness landscape in an enzyme active site.
    Article Snippet: .. For the pooledculture enrichment assays, Tm9D8* was cloned into the arabinose- inducible pBAD24 backbone while for in vitro assays and protein expression for purification, the pET22b(+) backbone was used. pBAD24- sfGFPx1 was a gift from Sankar Adhya & Francisco Malagon (Addgene plasmid # 51558; http://n2t.net/ addgene:51558; RRID:Addgene_51558) (53). ..

    Plasmid Preparation:

    Article Title: A combinatorially complete epistatic fitness landscape in an enzyme active site
    Article Snippet: .. For the pooled-culture enrichment assays, Tm 9D8* was cloned into the arabinose-inducible pBAD24 backbone while for in vitro assays and protein expression for purification, the pET22b(+) backbone was used. pBAD24-sfGFPx1 was a gift from Sankar Adhya & Francisco Malagon (Addgene plasmid # 51558; http://n2t.net/addgene:51558 ; RRID:Addgene_51558) ( ). ..

    Article Title: A combinatorially complete epistatic fitness landscape in an enzyme active site.
    Article Snippet: .. For the pooledculture enrichment assays, Tm9D8* was cloned into the arabinose- inducible pBAD24 backbone while for in vitro assays and protein expression for purification, the pET22b(+) backbone was used. pBAD24- sfGFPx1 was a gift from Sankar Adhya & Francisco Malagon (Addgene plasmid # 51558; http://n2t.net/ addgene:51558; RRID:Addgene_51558) (53). ..



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    Image Search Results


    Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in pET22b vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.

    Journal: Frontiers in Microbiology

    Article Title: Repurposing of FDA Approved Drugs Against SARS-CoV-2 Papain-Like Protease: Computational, Biochemical, and in vitro Studies

    doi: 10.3389/fmicb.2022.877813

    Figure Lengend Snippet: Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in pET22b vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.

    Article Snippet: The cDNA sequence coding for SCoV-2 PLpro was codon-optimised for bacterial expression and was custom-synthesised in pET22b vector backbone by GeneScript, USA.

    Techniques: Plasmid Preparation, Expressing, Synthesized, Purification