mbp mcherry expression plasmid (Addgene inc)
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Addgene inc
mbp mcherry expression plasmid
Mbp Mcherry Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet+vector/pET+MBP+mCherry+LIC+cloning+vector+(MBP-mCherry)+(Plasmid+%2329747)/pmc13020068-45-0-8
Average 94 stars, based on 4 article reviews
Mbp Mcherry Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet+vector/pET+MBP+mCherry+LIC+cloning+vector+(MBP-mCherry)+(Plasmid+%2329747)/pmc13020068-45-0-8
Average 94 stars, based on 4 article reviews
mbp mcherry expression plasmid - by Bioz Stars,
2026-09
94/100 stars
Images
1) Product Images from "Horizontal and vertical gene transfer shape the plasmid host range in surface-associated microbial systems"
Article Title: Horizontal and vertical gene transfer shape the plasmid host range in surface-associated microbial systems
Journal: iScience
doi: 10.1016/j.isci.2026.115299
Figure Legend Snippet: Hypothesis and experiment system (A) We hypothesize that vertical and horizontal gene transfer (VGT and HGT) are influenced by the characteristics of the potential recipient cell types and determine the proliferation and diversity of transconjugant cells. Because the potential recipient community comprises multiple cell types with varying growth traits and conjugation probabilities, we expect the resulting composition of transconjugant cells to be shaped by these cell type-specific traits. (B) Our experimental system consists of E . coli MG1655 lacI q -pLpp-mCherry as the plasmid donor strain and pB10 as the focal plasmid. pB10 donor cells express RFP from the chromosome and transconjugants express GFP from pB10.
Techniques Used: Conjugation Assay, Plasmid Preparation
Figure Legend Snippet: Transconjugant proportions and diversities after surface-associated conjugation assays for different environmental conditions (A) Proportion of transconjugant cells relative to total cells after surface-associated conjugation assays using the WWTP community as the potential recipient cell population. We conducted conjugation assays on 1×SWW, 10×SWW, or LB agar plates using E . coli MG1655 lacI q -pLpp-mCherry as the pB10 donor strain. (B) Relative abundances of bacterial class in the total potential recipient cell population (T) and the transconjugant cell population (TC) as identified by 16S rRNA gene sequencing. We separated and identified TC cells using FC-FACS-sorting of GFP-positive cells. (C) Normalized Shannon index of the transconjugant populations after surface-associated conjugation assays on 1×SWW, 10×SWW, or LB agar plates. We normalized the Shannon index of the TC populations to their corresponding T populations. (D) Principal coordinate analysis (PCoA) based on weighted UniFrac distances of T and TC populations after surface-associated conjugation assays on 1×SWW, 10×SWW, or LB agar plates. (E) Phylogenetic tree of transconjugant ASVs detected after surface-associated conjugation assays on 1×SWW, 10×SWW, or LB agar plates. The outer colored box denotes the bacterial phylum of each ASV, corresponding to the phylum-level groupings shown in panel (B). The inner heatmap box aligned with each tip shows the log 10 fold-changes in ASV abundance (TC relative to T) across the three conditions. For (A and C), each point is an independent biological replicate ( n = 3), horizontal bars are the means, error bars are ±1 standard deviation, and asterisks indicate statistically significant differences between the means based on two-way ANOVA with Holm correction (∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns = not significant). For (D), each point is an independent biological replicate ( n = 3).
Techniques Used: Conjugation Assay, Sequencing, Standard Deviation
Figure Legend Snippet: Transconjugant growth during surface-associated conjugation assays for different environmental conditions (A) Representative fluorescence microscopy images of transconjugant cells during surface-associated conjugation assays on LB agar plates. E . coli MG1655 lacI q -pLpp-mCherry is the pB10 donor strain and show red fluorescence. Transconjugant cells are green. The time indicated in the images refers to the point at which transconjugant cells first became detectable. (B) Normalized microcolony area ( A / a 0 ) plotted as a function of time during the surface-associated conjugation assays on LB agar plates. A is the total microcolony area and a 0 is the initial transconjugant area. Connected data points are for individual colonies ( n = 12). (C) Microcolony area at the endpoint of the mating assay (t = 24 h) for different environmental conditions. The half-violin and scatterplots present the sample distribution and individual microcolony measurements for surface-associated conjugation assays on different medium (n 1xSWW = 880, n 10xSWW = 664, n LB = 1,070, for microcolony number). We performed each experiment at least three independent experiments. Horizontal bars are the mean microcolony areas, error bars are the 99% confidence intervals, and asterisks indicate statistically significant differences between the means based on two-way ANOVA with Holm correction (∗∗ p < 0.01, ∗∗∗∗ p < 0.0001, ns = not significant).
Techniques Used: Conjugation Assay, Fluorescence, Microscopy
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Article Snippet: For expression of mini-Ph variants with the EH mutant SAM in E. coli, mini-Ph EH in a modified pET-3c vector was modified to replace PhL with PHC3L using HiFi Assembly cloning to generate mini-Ph PHC3L-EH , while mini-Ph SAMsurf-EH was assembled using restriction-ligation cloning. .. Linker SAM variants with N terminal HisSUMO-tag for expression in E. coli were assembled in a Article Title: Photoswitchable binders enable temporal dissection of endogenous protein function Article Snippet: Cloning was performed using standard molecular biology techniques to assemble Q5 DNA polymerase (NEB) amplified or synthesized (IDT) DNA fragments with NEB HiFi Assembly (NEB). .. DNA sequences encoding the extended caps were ordered as synthetic e-blocks (IDT) and inserted into two model DARPins: 1) 3G124 (anti-GFP) and 2) and Off7 (anti-MBP) and cloned into a Article Title: Backbone resonance assignments of the C-terminal region of human translation initiation factor eIF4B. 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Linker SAM variants with N terminal HisSUMO-tag were assembled in a Article Title: Insights into the compact CRISPR-Cas9d system. Article Snippet: .. The gene encoding the full-length Cas9d (Sangon) was codon optimized for E.coli expression and assembled into a modified Article Title: A disordered linker in the Polycomb protein Polyhomeotic tunes phase separation and oligomerization. Article Snippet: For expression of mini-Ph variants with the EH mutant SAM in E. coli, mini-Ph EH in a modified pET-3c vector was modified to replace PhL with PHC3L using HiFi Assembly cloning to generate mini-Ph PHC3L-EH , while mini-Ph SAMsurf-EH was assembled using restriction-ligation cloning. .. Linker SAM variants with N terminal HisSUMO-tag for expression in E. coli were assembled in a Article Title: Insights into the compact CRISPR–Cas9d system Article Snippet: .. The gene encoding the full-length Cas9d (Sangon) was codon optimized for E.coli expression and assembled into a modified Modification:Article Title: Insights into the compact CRISPR-Cas9d system. Article Snippet: .. The gene encoding the full-length Cas9d (Sangon) was codon optimized for E.coli expression and assembled into a modified Article Title: Insights into the compact CRISPR–Cas9d system Article Snippet: .. The gene encoding the full-length Cas9d (Sangon) was codon optimized for E.coli expression and assembled into a modified Article Title: Backbone resonance assignments of the C-terminal region of human translation initiation factor eIF4B. Article Snippet: Using the protein sequence of human eIF4B (Uniprot entry P23588), the corresponding DNA codon-optimized for E. coli was obtained (Integrated DNA Technologies). .. The C-terminal region (CTR; residues 333 to 611) was inserted into the SspI site of the modified Mutagenesis:Article Title: Insights into the compact CRISPR-Cas9d system. Article Snippet: .. The gene encoding the full-length Cas9d (Sangon) was codon optimized for E.coli expression and assembled into a modified Article Title: Increasing the versatility of the biphenyl-fused-dioxacyclodecyne class of strained alkynes Article Snippet: .. Method – Click Reactions for MS Detection Genetic Code Expanded GST Protein Purification An amber stop codon was introduced via site-directed mutagenesis into the F52 position of Glutathione S-Transferase (GST) tagged with a thrombin site-linked 6xHis-tag in a Cloning:Article Title: A disordered linker in the Polycomb protein Polyhomeotic tunes phase separation and oligomerization. Article Snippet: For expression of mini-Ph variants with the EH mutant SAM in E. coli, mini-Ph EH in a modified pET-3c vector was modified to replace PhL with PHC3L using HiFi Assembly cloning to generate mini-Ph PHC3L-EH , while mini-Ph SAMsurf-EH was assembled using restriction-ligation cloning. .. Linker SAM variants with N terminal HisSUMO-tag for expression in E. coli were assembled in a Article Title: Backbone resonance assignments of the C-terminal region of human translation initiation factor eIF4B. Article Snippet: Using the protein sequence of human eIF4B (Uniprot entry P23588), the corresponding DNA codon-optimized for E. coli was obtained (Integrated DNA Technologies). .. The C-terminal region (CTR; residues 333 to 611) was inserted into the SspI site of the modified Protein Purification:Article Title: Increasing the versatility of the biphenyl-fused-dioxacyclodecyne class of strained alkynes Article Snippet: .. Method – Click Reactions for MS Detection Genetic Code Expanded GST Protein Purification An amber stop codon was introduced via site-directed mutagenesis into the F52 position of Glutathione S-Transferase (GST) tagged with a thrombin site-linked 6xHis-tag in a Transformation Assay:Article Title: TMEM16 scramblases thin the membrane to enable lipid scrambling Article Snippet: .. Briefly, MSP1E3 in a Clone Assay:Article Title: Photoswitchable binders enable temporal dissection of endogenous protein function Article Snippet: Cloning was performed using standard molecular biology techniques to assemble Q5 DNA polymerase (NEB) amplified or synthesized (IDT) DNA fragments with NEB HiFi Assembly (NEB). .. DNA sequences encoding the extended caps were ordered as synthetic e-blocks (IDT) and inserted into two model DARPins: 1) 3G124 (anti-GFP) and 2) and Off7 (anti-MBP) and cloned into a Ligation:Article Title: Backbone resonance assignments of the C-terminal region of human translation initiation factor eIF4B. Article Snippet: Using the protein sequence of human eIF4B (Uniprot entry P23588), the corresponding DNA codon-optimized for E. coli was obtained (Integrated DNA Technologies). .. The C-terminal region (CTR; residues 333 to 611) was inserted into the SspI site of the modified |
