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pericyte marker cd13  (R&D Systems)


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    R&D Systems pericyte marker cd13
    Pericyte Marker Cd13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pericytes/Mouse+Aminopeptidase+N%2FCD13+Antibody/pm41828554-255-8-14
    Average 96 stars, based on 122 article reviews
    pericyte marker cd13 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Evolution of aberrant brain‐wide spatiotemporal dynamics of resting‐state networks in a Huntington's disease mouse model
    Article Snippet: After washing three times with PBS, permeabilisation and blocking of endogenous proteins were done with 0.5% Triton X‐100 and 10% normal donkey serum (NDS, Jackson Immunoresearch, 017‐000‐121) in PBS for 30 min, followed by donkey anti‐mouse IgG Fab fragment (1:40 in PBS, Jackson Immunoresearch, 715‐006‐151), blocking for 1 h at RT. .. Two stainings were performed where the following primary antibodies were applied in the first staining (1) 2B4 – marker for mHTT (1:200, Sigma Eldrich, Cat. # MAB5492) and CD13 – marker for pericytes (1:100, R&D Systems, Cat. # AF2335) diluted in PBS + 5% NDS and in the second staining (2) 2B4 (1:200, Sigma Eldrich, Cat. # MAB5492), GFAP – marker for reactive astrocytes (1:1000, Abcam, Cat. # ab4674) and VEGF (1:100, Thermo Fisher, Cat. # MA5‐32038) were diluted in 0.1% Triton X‐100 and 1% NDS in PBS and incubated overnight at RT. .. After washing with PBS, the fluorescent‐conjugated secondary antibodies were applied: (1) donkey anti‐mouse Cy5 (1:50, Jackson Immunoresearch, Cat. # 715‐175‐151) for 2B4 and donkey anti‐goat AF555 (1:200, Thermo Fisher, Cat. # A21432) for CD13 and (2) donkey anti‐chicken Cy3 (1:500, Jackson Immunoresearch, Cat. # 703‐166‐155), donkey anti‐mouse Cy5 (1:50, Jackson Immunoresearch, Cat. # 715‐175‐151) and donkey anti‐rabbit AF488 (1:200, Jackson Immunoresearch, 711‐545‐152) for VEGF with PBS for 1 h on RT.

    Article Title: Evolution of aberrant brain-wide spatiotemporal dynamics of resting-state networks in a Huntington's disease mouse model.
    Article Snippet: After washing three timeswith PBS, permeabilisation and blocking of endogenous proteins were done with 0.5% Triton X-100 and 10% normal donkey serum (NDS, Jackson Immunoresearch, 017-000-121) in PBS for 30min, followed by donkey anti-mouse IgG Fab fragment (1:40 in PBS, Jackson Immunoresearch, 715-006-151), blocking for 1 h at RT. .. Two stainings were performed where the following primary antibodies were applied in the first staining (1) 2B4 – marker for mHTT (1:200, Sigma Eldrich, Cat. # MAB5492) and CD13 – marker for pericytes (1:100, R&D Systems, Cat. # AF2335) diluted in PBS + 5% NDS and in the second staining (2) 2B4 (1:200, Sigma Eldrich, Cat. # MAB5492), GFAP – marker for reactive astrocytes (1:1000, Abcam, Cat. # ab4674) and VEGF (1:100, Thermo nloaded from https://onlinelibrary.w iley.com /doi/10.1002/ctm 2.70055 by U FSC - U niversidade Federal de Santa C atarina, W iley O nline L ibrary on [01/11/2024]. ..

    Marker:

    Article Title: Evolution of aberrant brain‐wide spatiotemporal dynamics of resting‐state networks in a Huntington's disease mouse model
    Article Snippet: After washing three times with PBS, permeabilisation and blocking of endogenous proteins were done with 0.5% Triton X‐100 and 10% normal donkey serum (NDS, Jackson Immunoresearch, 017‐000‐121) in PBS for 30 min, followed by donkey anti‐mouse IgG Fab fragment (1:40 in PBS, Jackson Immunoresearch, 715‐006‐151), blocking for 1 h at RT. .. Two stainings were performed where the following primary antibodies were applied in the first staining (1) 2B4 – marker for mHTT (1:200, Sigma Eldrich, Cat. # MAB5492) and CD13 – marker for pericytes (1:100, R&D Systems, Cat. # AF2335) diluted in PBS + 5% NDS and in the second staining (2) 2B4 (1:200, Sigma Eldrich, Cat. # MAB5492), GFAP – marker for reactive astrocytes (1:1000, Abcam, Cat. # ab4674) and VEGF (1:100, Thermo Fisher, Cat. # MA5‐32038) were diluted in 0.1% Triton X‐100 and 1% NDS in PBS and incubated overnight at RT. .. After washing with PBS, the fluorescent‐conjugated secondary antibodies were applied: (1) donkey anti‐mouse Cy5 (1:50, Jackson Immunoresearch, Cat. # 715‐175‐151) for 2B4 and donkey anti‐goat AF555 (1:200, Thermo Fisher, Cat. # A21432) for CD13 and (2) donkey anti‐chicken Cy3 (1:500, Jackson Immunoresearch, Cat. # 703‐166‐155), donkey anti‐mouse Cy5 (1:50, Jackson Immunoresearch, Cat. # 715‐175‐151) and donkey anti‐rabbit AF488 (1:200, Jackson Immunoresearch, 711‐545‐152) for VEGF with PBS for 1 h on RT.

    Article Title: Evolution of aberrant brain-wide spatiotemporal dynamics of resting-state networks in a Huntington's disease mouse model.
    Article Snippet: After washing three timeswith PBS, permeabilisation and blocking of endogenous proteins were done with 0.5% Triton X-100 and 10% normal donkey serum (NDS, Jackson Immunoresearch, 017-000-121) in PBS for 30min, followed by donkey anti-mouse IgG Fab fragment (1:40 in PBS, Jackson Immunoresearch, 715-006-151), blocking for 1 h at RT. .. Two stainings were performed where the following primary antibodies were applied in the first staining (1) 2B4 – marker for mHTT (1:200, Sigma Eldrich, Cat. # MAB5492) and CD13 – marker for pericytes (1:100, R&D Systems, Cat. # AF2335) diluted in PBS + 5% NDS and in the second staining (2) 2B4 (1:200, Sigma Eldrich, Cat. # MAB5492), GFAP – marker for reactive astrocytes (1:1000, Abcam, Cat. # ab4674) and VEGF (1:100, Thermo nloaded from https://onlinelibrary.w iley.com /doi/10.1002/ctm 2.70055 by U FSC - U niversidade Federal de Santa C atarina, W iley O nline L ibrary on [01/11/2024]. ..

    Incubation:

    Article Title: Evolution of aberrant brain‐wide spatiotemporal dynamics of resting‐state networks in a Huntington's disease mouse model
    Article Snippet: After washing three times with PBS, permeabilisation and blocking of endogenous proteins were done with 0.5% Triton X‐100 and 10% normal donkey serum (NDS, Jackson Immunoresearch, 017‐000‐121) in PBS for 30 min, followed by donkey anti‐mouse IgG Fab fragment (1:40 in PBS, Jackson Immunoresearch, 715‐006‐151), blocking for 1 h at RT. .. Two stainings were performed where the following primary antibodies were applied in the first staining (1) 2B4 – marker for mHTT (1:200, Sigma Eldrich, Cat. # MAB5492) and CD13 – marker for pericytes (1:100, R&D Systems, Cat. # AF2335) diluted in PBS + 5% NDS and in the second staining (2) 2B4 (1:200, Sigma Eldrich, Cat. # MAB5492), GFAP – marker for reactive astrocytes (1:1000, Abcam, Cat. # ab4674) and VEGF (1:100, Thermo Fisher, Cat. # MA5‐32038) were diluted in 0.1% Triton X‐100 and 1% NDS in PBS and incubated overnight at RT. .. After washing with PBS, the fluorescent‐conjugated secondary antibodies were applied: (1) donkey anti‐mouse Cy5 (1:50, Jackson Immunoresearch, Cat. # 715‐175‐151) for 2B4 and donkey anti‐goat AF555 (1:200, Thermo Fisher, Cat. # A21432) for CD13 and (2) donkey anti‐chicken Cy3 (1:500, Jackson Immunoresearch, Cat. # 703‐166‐155), donkey anti‐mouse Cy5 (1:50, Jackson Immunoresearch, Cat. # 715‐175‐151) and donkey anti‐rabbit AF488 (1:200, Jackson Immunoresearch, 711‐545‐152) for VEGF with PBS for 1 h on RT.

    Data-independent acquisition:

    Article Title: Ang-2/VEGF bispecific antibody reprograms macrophages and resident microglia to anti-tumor phenotype and prolongs glioblastoma survival
    Article Snippet: To prevent nonspecific staining, sections were incubated with 5% normal donkey serum in PBS (Jackson ImmunoResearch) before incubation with the respective primary and secondary antibodies. .. Endothelial cells were detected with an anti-CD31 mAb (DIA-310, 1:20; DIANOVA), and desmin-positive pericytes were detected using the mAb AF3844 (1:500; R&D Systems). .. Secondary fluorescent antibodies were used at a concentration of 1:200 (Jackson ImmunoResearch).

    other:




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    Image Search Results


    ( A ) Schematic representation of the microvascular network-on-chip assay adapted from https://aimbiotech.com using https://biorender.com . After three days of vessel self-assembly, the microvessels were treated for another three days with TNF/Glc (TG). ( B ) Representative immunofluorescence pictures of VE-cadherin (green)-stained microvessels (scale-bar, 100µm) under sustained TG treatment showing increased endothelial sprout rupture (white rectangles) particularly when using male HUVECs in combination with human brain pericytes. ( C ) Representative 3D renderings of the segmented vasculogensis-on-chip assay used for quantifications. Single detected objects are colorcoded. ( D ) Quantifications of microvascularity showing surface area and objects counted (fem-EC-derived, red dots, N=14; male-EC-derived, blue dots, N=13). ( E-G ) Gene expression analysis of pericytes after exposure of conditioned medium derived from female (red) or male (blue) ECs showing increase in the inflammatory and fibrotic response, differential TEAD response and sex-specific differences in the expression of NOX4 under TG ( E ). ( F,G ) QRT-PCR analysis of pericytes after exposure of conditioned medium from ECs with silenced YAP/TAZ or with transiently transfected YAP-5SA or TAZ-S89A for CXCL8 ( F ) and PAI1 ( G ). Data represent N=3-8, analyzed from three independent experiments and displayed as mean ± SEM. One-way analysis of variance (ANOVA) followed by multiple-comparisons test. P values < 0.05 (*), <0.005 (**), <0.0005 (***), <0.0001 (****).

    Journal: bioRxiv

    Article Title: Endothelial YAP/TAZ rewiring under cardiometabolic stress drives sex-divergent vascular remodeling in heart failure with preserved ejection fraction

    doi: 10.64898/2026.05.07.723127

    Figure Lengend Snippet: ( A ) Schematic representation of the microvascular network-on-chip assay adapted from https://aimbiotech.com using https://biorender.com . After three days of vessel self-assembly, the microvessels were treated for another three days with TNF/Glc (TG). ( B ) Representative immunofluorescence pictures of VE-cadherin (green)-stained microvessels (scale-bar, 100µm) under sustained TG treatment showing increased endothelial sprout rupture (white rectangles) particularly when using male HUVECs in combination with human brain pericytes. ( C ) Representative 3D renderings of the segmented vasculogensis-on-chip assay used for quantifications. Single detected objects are colorcoded. ( D ) Quantifications of microvascularity showing surface area and objects counted (fem-EC-derived, red dots, N=14; male-EC-derived, blue dots, N=13). ( E-G ) Gene expression analysis of pericytes after exposure of conditioned medium derived from female (red) or male (blue) ECs showing increase in the inflammatory and fibrotic response, differential TEAD response and sex-specific differences in the expression of NOX4 under TG ( E ). ( F,G ) QRT-PCR analysis of pericytes after exposure of conditioned medium from ECs with silenced YAP/TAZ or with transiently transfected YAP-5SA or TAZ-S89A for CXCL8 ( F ) and PAI1 ( G ). Data represent N=3-8, analyzed from three independent experiments and displayed as mean ± SEM. One-way analysis of variance (ANOVA) followed by multiple-comparisons test. P values < 0.05 (*), <0.005 (**), <0.0005 (***), <0.0001 (****).

    Article Snippet: HUVECs from pooled donors (PromoCell; pooled from single-female donors: C-12200, 479Z024, 485Z034, 488Z021, 433Z035.1 ; male-pool: C-12203, 479Z016) and human brain pericytes (ScienCell, Reference: #1200, 27194, single donor) were cultured on 0.2% gelatine precoated dishes/flasks in EGM2-Bulletkit without antibiotics (Lonza, #CC-3156) or in pericyte medium (PM, ScienCell, #1201), respectively.

    Techniques: Immunofluorescence, Staining, Derivative Assay, Gene Expression, Expressing, Quantitative RT-PCR, Transfection

    Schematic representation of the modulation of tissue microenvironments in the lung ( a ) and brain ( b ) by pericyte-derived factors. Created in BioRender. Rasouli, S. J. (2026) https://BioRender.com/pf1pwy6 , licensed under CC BY 4.0.

    Journal: Nature Communications

    Article Title: Pericytes are organ-specific regulators of tissue morphogenesis

    doi: 10.1038/s41467-026-71643-1

    Figure Lengend Snippet: Schematic representation of the modulation of tissue microenvironments in the lung ( a ) and brain ( b ) by pericyte-derived factors. Created in BioRender. Rasouli, S. J. (2026) https://BioRender.com/pf1pwy6 , licensed under CC BY 4.0.

    Article Snippet: Taken together, these findings indicate that the loss of Nodal can induce reactive astrogliosis directly, consistent with an angiocrine signaling role of the pericyte-derived TGFβ family ligand.

    Techniques: Derivative Assay