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gene exp per2 mm00478099 m1  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene exp per2 mm00478099 m1
    Gene Exp Per2 Mm00478099 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/per2/Gene+Exp%2E+Per2%2C+Mm00478099_m1/pmc13112403-357-25--1
    Average 94 stars, based on 1 article reviews
    gene exp per2 mm00478099 m1 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Cortisol Circadian Rhythm and Insulin Resistance in Muscle: Effect of Dosing and Timing of Hydrocortisone Exposure on Insulin Sensitivity in Synchronized Muscle Cells.
    Article Snippet: Received: 6/24/2020 Accepted: 10/29/2020 Published(online): 10/30/2020 --------------CORTISOL CIRCADIAN RHYTHM AND INSULIN RESISTANCE IN MUSCLE: EFFECT OF DOSING AND TIMING OF HYDROCORTISONE EXPOSURE ON INSULIN SENSITIVITY IN SYNCHRONIZED MUSCLE CELLS.. Negri M. Pivonello C. Simeoli C. Di Gennaro G. Venneri M.A.. Sciarra F. Ferrigno R. de Angelis C. Sbardella E. De Martino M.C.

    Article Title: Postischemic application of estrogen ameliorates myocardial damage in an in vivo mouse model.
    Article Snippet: Background: Cardioprotection provided by estrogen has been recognized for many years.. It is noteworthy that most of these studies employ a means of preinjury application in experimental research and the preventive usage in clinical studies.. Compared to pretreatment, postischemic administration of estrogen will be more practical in treating myocardial ischemia.

    Expressing:

    Article Title: Cortisol Circadian Rhythm and Insulin Resistance in Muscle: Effect of Dosing and Timing of Hydrocortisone Exposure on Insulin Sensitivity in Synchronized Muscle Cells.
    Article Snippet: Received: 6/24/2020 Accepted: 10/29/2020 Published(online): 10/30/2020 --------------CORTISOL CIRCADIAN RHYTHM AND INSULIN RESISTANCE IN MUSCLE: EFFECT OF DOSING AND TIMING OF HYDROCORTISONE EXPOSURE ON INSULIN SENSITIVITY IN SYNCHRONIZED MUSCLE CELLS.. Negri M. Pivonello C. Simeoli C. Di Gennaro G. Venneri M.A.. Sciarra F. Ferrigno R. de Angelis C. Sbardella E. De Martino M.C.

    Membrane:

    Article Title: Postischemic application of estrogen ameliorates myocardial damage in an in vivo mouse model.
    Article Snippet: Background: Cardioprotection provided by estrogen has been recognized for many years.. It is noteworthy that most of these studies employ a means of preinjury application in experimental research and the preventive usage in clinical studies.. Compared to pretreatment, postischemic administration of estrogen will be more practical in treating myocardial ischemia.

    Incubation:

    Article Title: Postischemic application of estrogen ameliorates myocardial damage in an in vivo mouse model.
    Article Snippet: Background: Cardioprotection provided by estrogen has been recognized for many years.. It is noteworthy that most of these studies employ a means of preinjury application in experimental research and the preventive usage in clinical studies.. Compared to pretreatment, postischemic administration of estrogen will be more practical in treating myocardial ischemia.

    Article Title: Inhibition of casein kinase 1 δ/ε improves cognitive performance in adult C57BL/6J mice.
    Article Snippet: Membranes were blocked for 45 min in 5% milk in tris buffered saline containing 0.1% tween-20 solution (Boston Bioproducts, Ashland, MA). .. Membranes were incubated overnight at 4 °C in specific antibodies to pERK p44/42, GSK3β, Arg3.1(Arc) (1:1000, Cell Signaling Technology, MA, USA) PER1 (1:1000 Invitrogen, CA, USA), PER2 (1:250 Invitrogen), or BMAL1 (1:1000 Bethyl Laboratories, TX, USA) or for 2 h at RT in antibody specific to β -actin(SigmaAldritch, MO, USA) in tris buffered saline with 0.1% tween20 (TBS-T) with 2% milk, washed 3 × 10 min in TBS-T, and incubated for 1 h at room temperature in horseradish peroxidase-conjugated secondary antibody (1:7500, SouthernBiotech, AL, USA), in TBS-T with 2% milk. .. After washing with TBS-T, bands were visualized with enhanced chemiluminescence (ECL; Thermo scientific) using an image analyzer (Amersham imager 600).

    Gentle:

    Article Title: Postischemic application of estrogen ameliorates myocardial damage in an in vivo mouse model.
    Article Snippet: Background: Cardioprotection provided by estrogen has been recognized for many years.. It is noteworthy that most of these studies employ a means of preinjury application in experimental research and the preventive usage in clinical studies.. Compared to pretreatment, postischemic administration of estrogen will be more practical in treating myocardial ischemia.

    Immunohistochemical staining:

    Article Title: Fkbp5 gene deletion: Circadian rhythm profile and brain proteomics in aged mice
    Article Snippet: .. Immunohistochemical staining was performed as previously described (Blair et al., ) using the following primary antibodies: PER1 (AB2201, MilliporeSigma, Burlington, MA, USA; 1:1000), PER2 (PA5‐100107, Invitrogen, Waltham, MA, USA; 1:1000), BMAL1 (14020S, Cell Signaling Technology, Danvers, MA, USA; 1:1000) CLOCK (PA1‐520 Invitrogen; 1:3000), and CK1δ (PA5‐32129, Invitrogen; 1:250). .. Stained tissues were mounted onto slides, dehydrated in alcohol gradients, and coverslipped with DPX mountant (Sigma‐Aldrich, St. Louis, MO, USA).

    Article Title: Fkbp5 gene deletion: Circadian rhythm profile and brain proteomics in aged mice.
    Article Snippet: .. Immunohistochemical staining was performed as previously described (Blair et al., 2019) using the following primary antibodies: PER1 (AB2201, MilliporeSigma, Burlington, MA, USA; 1:1000), PER2 (PA5- 100107, Invitrogen, Waltham, MA, USA; 1:1000), BMAL1 (14020S, Cell Signaling Technology, Danvers, MA, USA; 1:1000) CLOCK (PA1- 520 Invitrogen; 1:3000), and CK1δ (PA532129, Invitrogen; 1:250). .. Stained tissues were mounted onto slides, dehydrated in alcohol gradients, and coverslipped with DPX mountant (Sigma- Aldrich, St. Louis, MO, USA).

    Staining:

    Article Title: Fkbp5 gene deletion: Circadian rhythm profile and brain proteomics in aged mice
    Article Snippet: .. Immunohistochemical staining was performed as previously described (Blair et al., ) using the following primary antibodies: PER1 (AB2201, MilliporeSigma, Burlington, MA, USA; 1:1000), PER2 (PA5‐100107, Invitrogen, Waltham, MA, USA; 1:1000), BMAL1 (14020S, Cell Signaling Technology, Danvers, MA, USA; 1:1000) CLOCK (PA1‐520 Invitrogen; 1:3000), and CK1δ (PA5‐32129, Invitrogen; 1:250). .. Stained tissues were mounted onto slides, dehydrated in alcohol gradients, and coverslipped with DPX mountant (Sigma‐Aldrich, St. Louis, MO, USA).

    Article Title: Fkbp5 gene deletion: Circadian rhythm profile and brain proteomics in aged mice.
    Article Snippet: .. Immunohistochemical staining was performed as previously described (Blair et al., 2019) using the following primary antibodies: PER1 (AB2201, MilliporeSigma, Burlington, MA, USA; 1:1000), PER2 (PA5- 100107, Invitrogen, Waltham, MA, USA; 1:1000), BMAL1 (14020S, Cell Signaling Technology, Danvers, MA, USA; 1:1000) CLOCK (PA1- 520 Invitrogen; 1:3000), and CK1δ (PA532129, Invitrogen; 1:250). .. Stained tissues were mounted onto slides, dehydrated in alcohol gradients, and coverslipped with DPX mountant (Sigma- Aldrich, St. Louis, MO, USA).

    Saline:

    Article Title: Inhibition of casein kinase 1 δ/ε improves cognitive performance in adult C57BL/6J mice.
    Article Snippet: Membranes were blocked for 45 min in 5% milk in tris buffered saline containing 0.1% tween-20 solution (Boston Bioproducts, Ashland, MA). .. Membranes were incubated overnight at 4 °C in specific antibodies to pERK p44/42, GSK3β, Arg3.1(Arc) (1:1000, Cell Signaling Technology, MA, USA) PER1 (1:1000 Invitrogen, CA, USA), PER2 (1:250 Invitrogen), or BMAL1 (1:1000 Bethyl Laboratories, TX, USA) or for 2 h at RT in antibody specific to β -actin(SigmaAldritch, MO, USA) in tris buffered saline with 0.1% tween20 (TBS-T) with 2% milk, washed 3 × 10 min in TBS-T, and incubated for 1 h at room temperature in horseradish peroxidase-conjugated secondary antibody (1:7500, SouthernBiotech, AL, USA), in TBS-T with 2% milk. .. After washing with TBS-T, bands were visualized with enhanced chemiluminescence (ECL; Thermo scientific) using an image analyzer (Amersham imager 600).



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