Journal: bioRxiv

Article Title: Celiac disease patient derived iPSC–small intestinal epithelial cells are more persistent under cytokine stimuli than healthy control cells

doi: 10.64898/2026.03.12.710771

Figure Lengend Snippet: Before differentiation, both iPSC lines expressed the as well as the Nanog gene and protein (n = 3), and POU5F1 / Oct3/4 gene and protein (n = 3) (panel A). At the DE stage, cultures expressed the FOXA2 and SOX17 gene and protein (n = 3; panel B, FOXA2 red, SOX17 green, DAPI blue, 100 μm). At the PDE stage, cultures expressed the Cdx2 and SOX17 gene and protein (n = 3; panel C, Cdx2 red, SOX17 green, DAPI blue, scale bar 100 μm). Microscope images (A-C) were taken with an Evident-Olympus IX83 microscope 20x lens using a Hamamatsu ORCA-Fusion C14440-20UP camera and NEO LiveImaging software. Intestinal epithelial cell specific functionality assessed with PEPT1 dipeptide uptake assay (D). On left there is image captured above the cellular level and on the right, there is image taken from the level which is in the middle of nucleus (n=3; dipeptide D-Ala-Leu-Lys-AMCA green, DAPI blue, 20 μm). Confocal images were taken with a Zeiss LSM780 laser scanning microscope with a 40x lens and ZEN Blue 3.6 software.

Article Snippet: For uptake analysis the The fluorescence-labelled tripeptide substrate of PEPT1 (β-Ala-Lys (β-Ala-Lys-AMCA), Peptide institute, Osaka, Japan) was diluted to HBSS in the concentration 25μM and incubated with cells for 4h in the incubator.

Techniques: Microscopy, Software, Laser-Scanning Microscopy

Journal: bioRxiv

Article Title: Celiac disease patient derived iPSC–small intestinal epithelial cells are more persistent under cytokine stimuli than healthy control cells

doi: 10.64898/2026.03.12.710771

Figure Lengend Snippet: SIECs derived from celiac (CeD) and healthy control (Contr.) iPSCs and matured for 26 days were stimulated with IFNγ (10 ng/mL and 50 ng/mL) or TNFα (10 ng/mL) for 48 h. A) Immunofluorescence staining for PEPT1 (green), filamentous actin (phalloidin, red), and nuclei (DAPI, blue). B) PEPT1 expression quantified by qRT-PCR (CeD n = 6, Contr. n = 3). Confocal images were taken with a Zeiss LSM800 laser scanning microscope with a 63x lens and ZEN Blue 3.6 software. C) Cytotoxicity assessed based on lactate dehydrogenase (LDH) activity in culture media after 48 h of cytokine exposure compared to unstimulated controls (n = 3). Statistical significance * = p ≤ 0.05; * = p ≤ 0.005, and *** = p ≤ 0.0005.

Article Snippet: For uptake analysis the The fluorescence-labelled tripeptide substrate of PEPT1 (β-Ala-Lys (β-Ala-Lys-AMCA), Peptide institute, Osaka, Japan) was diluted to HBSS in the concentration 25μM and incubated with cells for 4h in the incubator.

Techniques: Derivative Assay, Control, Immunofluorescence, Staining, Expressing, Quantitative RT-PCR, Laser-Scanning Microscopy, Software, Activity Assay

Journal: Journal of Physiology and Biochemistry

Article Title: Modulation of SIRT1/PPARγ pathways and tight junction proteins by nicotinamide riboside under chronic variable stress

doi: 10.1007/s13105-026-01153-7

Figure Lengend Snippet: The effects of different nicotinamide riboside doses on the levels of PepT1 ( A ), LAT2 ( B ), EAAT3 ( C ), FABP2 ( D ), and FATP4 ( E ) proteins in the jejunum tissue of rats under normal and chronic variable stress (CVS) conditions. Western blot analysis was performed using β-actin as a loading control to ensure equal protein loading. All bands are shown in (G). Different letters (a–e) indicate statistically significant differences between groups ( p < 0.05) according to Tukey’s post hoc test following two-way ANOVA. Two-way ANOVA revealed significant effects for PepT1 (Condition p < 0.0001; NR p = 0.0001; Condition x NR p < 0.0001), LAT2 (Condition p < 0.0001; NR p = 0.0002; Condition x NR p = 0.0007), EAAT3 (Condition p < 0.0001; NR p < 0.0001; Condition x NR p < 0.0001), FABP2 (Condition p < 0.0001; NR p < 0.0001; Condition x NR p = 0.0236), and FATP4 (Condition p < 0.0001; NR p < 0.0001; Condition x NR p = 0.3521). Comprehensive statistical data are presented in Supplementary Table and full immunoblots images are shown in supplementary Fig.

Article Snippet: Membranes were blocked with 5% bovine serum albumin (BSA) for 2 h at room temperature and incubated overnight at 4 °C with primary antibodies as follows: PPARγ (sc-271392), GLUT2 (sc-518022), IRS-1 (sc-8038), SIRT1 (sc-74465), FASN (sc-48357), claudin-1 (sc-166338), claudin-4 (sc-376643), occludin (sc-133256), ZO-1 (sc-33725), MUC2 (sc-53381), PepT1 (sc-373742), LAT2 (sc-293242), FABP2 (sc-374482), FATP4 (sc-393309) and β-Actin (sc-517582) (all from Santa Cruz Biotechnology); SGLT1 (DF7202-100; Affinity Biosciences); EAAT3 (orb1161904; Biorbyt Ltd., Cambridge, UK).

Techniques: Western Blot, Control

Journal: Animal Nutrition

Article Title: New functions of enzymatic cottonseed protein: Improvement of intestinal digestion and absorption, structure and microbial composition of juvenile yellow catfish ( Pelteobagrus fulvidraco )

doi: 10.1016/j.aninu.2025.03.022

Figure Lengend Snippet: Effects of different levels of dietary enzymatic cottonseed protein (ECP) on the mRNA expression level of transporters (A) and the protein expression level of PepT1 (B). NP = normal protein (42% CP) diet; ECP0 = supplement 0% ECP to low protein (LP) (39% CP) diet; ECP1 = supplement 1% ECP to LP diet; ECP2 = supplement 2% ECP to LP diet; ECP3 = supplement 3% ECP to LP diet; ECP4 = supplement 4% ECP to LP diet; ECP5 = supplement 5% ECP to LP diet. One-way ANOVA was used for data from the ECP0-5 groups, and data from the NP group were compared with those from the ECP0 and ECP2 groups using Independent Samples T -tests. Mean values from the ECP0-5 groups with different letters were significantly different ( P < 0.05); ∗ indicated a significant difference between the NP group and ECP0 group ( P < 0.05) Values are means ( n = 6).

Article Snippet: It was then incubated for 17 h at 4 °C with primary antibodies including PepT1 (ER64550), caudal type homeobox transcription factor 2 (CDX2) (ET1605-4), PDZ domain containing 1 (PDZK1) (HA722729), and Rho-associated kinase 1 (ROCK1) (ER40122) from Huabio Biotechnology Co., Ltd., Hangzhou, Zhejiang, China, and specificity protein 1 (SP1) (A14662) and sodium/hydrogen exchange isoform 3 (NHE3) (A17591) from ABclonal Biotechnology Co., Ltd., Wuhan, Hubei, China.

Techniques: Expressing

Journal: Animal Nutrition

Article Title: New functions of enzymatic cottonseed protein: Improvement of intestinal digestion and absorption, structure and microbial composition of juvenile yellow catfish ( Pelteobagrus fulvidraco )

doi: 10.1016/j.aninu.2025.03.022

Figure Lengend Snippet: Effects of different levels of dietary enzymatic cottonseed protein (ECP) on the genes nrf2 (A) and jak2 (B), and proteins SP1 (C), CDX2 (D), NHE3 (E), and PDZK1 (F) expression of PepT1-related. NP = normal protein (42% CP) diet; ECP0 = supplement 0% ECP to low protein (LP) (39% CP) diet; ECP1 = supplement 1% ECP to LP diet; ECP2 = supplement 2% ECP to LP diet; ECP3 = supplement 3% ECP to LP diet; ECP4 = supplement 4% ECP to LP diet; ECP5 = supplement 5% ECP to LP diet. One-way ANOVA was used for data from the ECP0-5 groups, and data from the NP group were compared with those from the ECP0 and ECP2 groups using Independent Samples T -tests. Mean values from the ECP0-5 groups with different letters were significantly different ( P < 0.05); ∗ indicated a significant difference between the NP group and ECP0 group ( P < 0.05); # indicated a significant difference between the ECP0 group and ECP2 group ( P < 0.05). Values are means ( n = 6).

Article Snippet: It was then incubated for 17 h at 4 °C with primary antibodies including PepT1 (ER64550), caudal type homeobox transcription factor 2 (CDX2) (ET1605-4), PDZ domain containing 1 (PDZK1) (HA722729), and Rho-associated kinase 1 (ROCK1) (ER40122) from Huabio Biotechnology Co., Ltd., Hangzhou, Zhejiang, China, and specificity protein 1 (SP1) (A14662) and sodium/hydrogen exchange isoform 3 (NHE3) (A17591) from ABclonal Biotechnology Co., Ltd., Wuhan, Hubei, China.

Techniques: Expressing