Journal: Journal of Physiology and Biochemistry

Article Title: Modulation of SIRT1/PPARγ pathways and tight junction proteins by nicotinamide riboside under chronic variable stress

doi: 10.1007/s13105-026-01153-7

Figure Lengend Snippet: The effects of different nicotinamide riboside doses on the levels of PepT1 ( A ), LAT2 ( B ), EAAT3 ( C ), FABP2 ( D ), and FATP4 ( E ) proteins in the jejunum tissue of rats under normal and chronic variable stress (CVS) conditions. Western blot analysis was performed using β-actin as a loading control to ensure equal protein loading. All bands are shown in (G). Different letters (a–e) indicate statistically significant differences between groups ( p < 0.05) according to Tukey’s post hoc test following two-way ANOVA. Two-way ANOVA revealed significant effects for PepT1 (Condition p < 0.0001; NR p = 0.0001; Condition x NR p < 0.0001), LAT2 (Condition p < 0.0001; NR p = 0.0002; Condition x NR p = 0.0007), EAAT3 (Condition p < 0.0001; NR p < 0.0001; Condition x NR p < 0.0001), FABP2 (Condition p < 0.0001; NR p < 0.0001; Condition x NR p = 0.0236), and FATP4 (Condition p < 0.0001; NR p < 0.0001; Condition x NR p = 0.3521). Comprehensive statistical data are presented in Supplementary Table and full immunoblots images are shown in supplementary Fig.

Article Snippet: Membranes were blocked with 5% bovine serum albumin (BSA) for 2 h at room temperature and incubated overnight at 4 °C with primary antibodies as follows: PPARγ (sc-271392), GLUT2 (sc-518022), IRS-1 (sc-8038), SIRT1 (sc-74465), FASN (sc-48357), claudin-1 (sc-166338), claudin-4 (sc-376643), occludin (sc-133256), ZO-1 (sc-33725), MUC2 (sc-53381), PepT1 (sc-373742), LAT2 (sc-293242), FABP2 (sc-374482), FATP4 (sc-393309) and β-Actin (sc-517582) (all from Santa Cruz Biotechnology); SGLT1 (DF7202-100; Affinity Biosciences); EAAT3 (orb1161904; Biorbyt Ltd., Cambridge, UK).

Techniques: Western Blot, Control

Journal: Antioxidants

Article Title: The Cytotoxicity of Doxorubicin Can Be Accelerated by a Combination of Hyperthermia and 5-Aminolevulinic Acid

doi: 10.3390/antiox10101531

Figure Lengend Snippet: Western blot analysis of each transporter expression. SLC22A16 ( A , B ), ABCG2 ( C , D ) and SLC15A1 ( E , F ) were normalized to β-actin expression. Data are expressed as the mean ± SD ( n = 3). * p < 0.05, ** p < 0.01. Student’s t -test.

Article Snippet: Antirabbit SLC22A16 (cat. no. LS-C176922) (LSBio, Seattle, WA, USA), ABCG2 (cat. no. 42078) (Cell Signaling Technology Japan K.K., Tokyo, Japan), or SLC15A1 (cat. no. bs-10588R) (Bioss, Boston, MA, USA) antibodies (1:1000) were added to the Can Get Signal ® Immunoreaction Enhancer Solution 1 (Toyobo Co. Ltd., Osaka, Japan).

Techniques: Western Blot, Expressing

Journal: Nature communications

Article Title: Physiological and therapeutic regulation of glucose homeostasis by upper small intestinal PepT1-mediated protein sensing.

doi: 10.1038/s41467-018-03490-8

Figure Lengend Snippet: Fig. 2 Inhibition of peptide transporter 1 (PepT1) reverses the ability of casein infusion to increase glucose tolerance in healthy rodents. Percentage of change in plasma glucose levels (a), integrated area under the curve (AUC, b), and plasma insulin levels (c) over time during the IVGTT for healthy rats that received an upper small intestinal infusion of saline (n = 18), casein (n = 24), saline+the competitive PepT1 antagonist 4-aminomethylbenzoic acid (4- AMBA, n = 7), or casein+4-AMBA (n = 6). Values are presented as mean ± s.e.m., where asterisk (*) represents p < 0.05 compared to saline control and hash (#) represents p < 0.05 compared to casein+4-AMBA (assessed using ANOVA with Tukey post-hoc test)

Article Snippet: A subset of rats received upper small intestinal injections of purified lentiviral particles expressing mismatch or rat PepT1 shRNA (1 × 106 infection units, Santa Cruz, Dallas, TX, USA), which has previously been optimized to specifically infect the upper and not the lower small intestine42.

Techniques: Inhibition, Clinical Proteomics, Saline, Control

Journal: Nature communications

Article Title: Physiological and therapeutic regulation of glucose homeostasis by upper small intestinal PepT1-mediated protein sensing.

doi: 10.1038/s41467-018-03490-8

Figure Lengend Snippet: Fig. 3 Upper small intestinal casein infusion lowers glucose production through activation of PepT1 in healthy rodents. In vivo glucose kinetics were assessed in conscious, unrestrained healthy rats using the pancreatic (basal insulin) euglycemic clamp as outlined in a. Rates of glucose infusion (b), glucose production (GP, c), and glucose uptake (d) in rats that received an upper small intestinal (S.I). infusion of saline (n = 10) or casein (n = 9), intravenous (IV) infusion of casein (n = 5), upper small intestinal infusion of saline+4-AMBA (n = 6), casein+4-APAA (n = 5), or casein+4-AMBA (n = 6). Relative PepT1 mRNA expression in the mucosal layer isolated from S.I. segments ~6–10 cm (upper S.I., e) or ~25–30 cm (mid-S.I., f) distal to the pyloric sphincter in rats that received an upper S.I. lentiviral infection with control mismatch (n = 6) or PepT1 shRNA (n = 6) particles. Rates of glucose production (g) and glucose infusion (h) in rats that received mismatch shRNA lentiviral infection+saline infusion (n = 6), mismatch+casein infusion (n = 6), PepT1 shRNA lentiviral infection+saline infusion (n = 5), or PepT1 shRNA+casein infusion (n = 6). Rates of glucose infusion (i) and glucose production (j) in rats that received saline+the GLP-1 receptor antagonist exendin-9 (n = 3) or casein+exendin-9 (n = 6). Values are presented as mean ± s.e.m., where basal represents the average GP of t = 60–90, clamp represents the average GP of t = 190–200 and asterisk (*) represents p < 0.05 compared to saline control. Statistical significance was determined using an unpaired, two-tailed t-test (two groups) or ANOVA with Tukey post-hoc test (3+ groups)

Article Snippet: A subset of rats received upper small intestinal injections of purified lentiviral particles expressing mismatch or rat PepT1 shRNA (1 × 106 infection units, Santa Cruz, Dallas, TX, USA), which has previously been optimized to specifically infect the upper and not the lower small intestine42.

Techniques: Activation Assay, In Vivo, Saline, Expressing, Isolation, Infection, Control, shRNA, Two Tailed Test

Journal: Nature communications

Article Title: Physiological and therapeutic regulation of glucose homeostasis by upper small intestinal PepT1-mediated protein sensing.

doi: 10.1038/s41467-018-03490-8

Figure Lengend Snippet: Fig. 4 Upper small intestinal PepT1-mediated protein sensing is physiologically relevant during refeeding in healthy rats. Conscious, unrestrained healthy rats underwent a fasting–refeeding protocol in which a low protein (LP) or casein-enriched high protein (HP) diet was offered ad libitum as outlined in a. Percentage of change in plasma glucose levels and integrated area under the curve (b, c), cumulative food intake (d), and plasma insulin levels (e) were monitored in rats that received LP or HP chow with an upper small intestinal (S.I.) infusion of saline (LP: n = 7, HP: n = 9) or 4-AMBA (LP: n = 6, HP: n = 9). Values are presented as mean ± s.e.m., where asterisk (*) represents p < 0.05 compared to LP saline and hash (#) represents p < 0.05 compared to HP+4-AMBA. For the analysis of a single time point, ANOVA with Tukey post-hoc test was used to determine statistical significance

Article Snippet: A subset of rats received upper small intestinal injections of purified lentiviral particles expressing mismatch or rat PepT1 shRNA (1 × 106 infection units, Santa Cruz, Dallas, TX, USA), which has previously been optimized to specifically infect the upper and not the lower small intestine42.

Techniques: Clinical Proteomics, Saline

Journal: Poultry Science

Article Title: The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers

doi: 10.1016/j.psj.2023.102477

Figure Lengend Snippet: Primer sequences for real-time PCR amplification.

Article Snippet: The primary antibodies and dilution rates were as follows: ZnT1, ab214356, 1:1000 (Abcam, Cambridge, UK); ZnT4, BA3484, 1:1000 (Boster, Wuhan, China); Zn transporter 5 (ZnT5), 25604-1-AP, 1:1000 (Proteintech, Chicago); ZnT7, 13966-1-AP, 1:1000 (Proteintech, Chicago, IL); ZnT9, BS62531, 1:1000 (Bioworld, Minneapolis, MN); Zn transporter 10 ( ZnT10 ), PA5-49188, 1:1000 (Invitrogen, CA); ZIP3, ab254868, 1:500 (Abcam, Cambridge, UK); ZIP5, ab105194, 1:1000 (Abcam, Cambridge, UK); B 0 AT1, ab180516, 1:1000 (Abcam, Cambridge, UK); LAT1, DF8065, 1:500 (Affinity Biosciences, Melbourne, Australia); y+LAT2, 13823-1-AP, 1:1000 (Proteintech, Chicago); rBAT, DF7379, 1:1000 (Affinity Biosciences, Melbourne, Australia) and PepT1, A03672-1, 1:500 (Boster, Wuhan, China).

Techniques: Real-time Polymerase Chain Reaction, Amplification

Journal: Poultry Science

Article Title: The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers

doi: 10.1016/j.psj.2023.102477

Figure Lengend Snippet: Effect of dietary Zn source on the mRNA expression levels of Zn, amino acid and small peptide transporters in the jejunum of broilers at 28 and 39 d of age. 1

Article Snippet: The primary antibodies and dilution rates were as follows: ZnT1, ab214356, 1:1000 (Abcam, Cambridge, UK); ZnT4, BA3484, 1:1000 (Boster, Wuhan, China); Zn transporter 5 (ZnT5), 25604-1-AP, 1:1000 (Proteintech, Chicago); ZnT7, 13966-1-AP, 1:1000 (Proteintech, Chicago, IL); ZnT9, BS62531, 1:1000 (Bioworld, Minneapolis, MN); Zn transporter 10 ( ZnT10 ), PA5-49188, 1:1000 (Invitrogen, CA); ZIP3, ab254868, 1:500 (Abcam, Cambridge, UK); ZIP5, ab105194, 1:1000 (Abcam, Cambridge, UK); B 0 AT1, ab180516, 1:1000 (Abcam, Cambridge, UK); LAT1, DF8065, 1:500 (Affinity Biosciences, Melbourne, Australia); y+LAT2, 13823-1-AP, 1:1000 (Proteintech, Chicago); rBAT, DF7379, 1:1000 (Affinity Biosciences, Melbourne, Australia) and PepT1, A03672-1, 1:500 (Boster, Wuhan, China).

Techniques: Expressing, Control

Journal: Poultry Science

Article Title: The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers

doi: 10.1016/j.psj.2023.102477

Figure Lengend Snippet: Effect of dietary Zn source on the mRNA expression levels of Zn, amino acid and small peptide transporters in the ileum of broilers at 28 and 39 d of age. 1

Article Snippet: The primary antibodies and dilution rates were as follows: ZnT1, ab214356, 1:1000 (Abcam, Cambridge, UK); ZnT4, BA3484, 1:1000 (Boster, Wuhan, China); Zn transporter 5 (ZnT5), 25604-1-AP, 1:1000 (Proteintech, Chicago); ZnT7, 13966-1-AP, 1:1000 (Proteintech, Chicago, IL); ZnT9, BS62531, 1:1000 (Bioworld, Minneapolis, MN); Zn transporter 10 ( ZnT10 ), PA5-49188, 1:1000 (Invitrogen, CA); ZIP3, ab254868, 1:500 (Abcam, Cambridge, UK); ZIP5, ab105194, 1:1000 (Abcam, Cambridge, UK); B 0 AT1, ab180516, 1:1000 (Abcam, Cambridge, UK); LAT1, DF8065, 1:500 (Affinity Biosciences, Melbourne, Australia); y+LAT2, 13823-1-AP, 1:1000 (Proteintech, Chicago); rBAT, DF7379, 1:1000 (Affinity Biosciences, Melbourne, Australia) and PepT1, A03672-1, 1:500 (Boster, Wuhan, China).

Techniques: Expressing, Control

Journal: Poultry Science

Article Title: The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers

doi: 10.1016/j.psj.2023.102477

Figure Lengend Snippet: Effect of dietary Zn source on the protein expression levels of Zn, amino acid and small peptide transporters in the jejunum of broilers at 28 and 39 d of age. 1

Article Snippet: The primary antibodies and dilution rates were as follows: ZnT1, ab214356, 1:1000 (Abcam, Cambridge, UK); ZnT4, BA3484, 1:1000 (Boster, Wuhan, China); Zn transporter 5 (ZnT5), 25604-1-AP, 1:1000 (Proteintech, Chicago); ZnT7, 13966-1-AP, 1:1000 (Proteintech, Chicago, IL); ZnT9, BS62531, 1:1000 (Bioworld, Minneapolis, MN); Zn transporter 10 ( ZnT10 ), PA5-49188, 1:1000 (Invitrogen, CA); ZIP3, ab254868, 1:500 (Abcam, Cambridge, UK); ZIP5, ab105194, 1:1000 (Abcam, Cambridge, UK); B 0 AT1, ab180516, 1:1000 (Abcam, Cambridge, UK); LAT1, DF8065, 1:500 (Affinity Biosciences, Melbourne, Australia); y+LAT2, 13823-1-AP, 1:1000 (Proteintech, Chicago); rBAT, DF7379, 1:1000 (Affinity Biosciences, Melbourne, Australia) and PepT1, A03672-1, 1:500 (Boster, Wuhan, China).

Techniques: Expressing, Control

Journal: Poultry Science

Article Title: The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers

doi: 10.1016/j.psj.2023.102477

Figure Lengend Snippet: Representative immunoblots of ZnT1, ZnT4, ZnT5, ZnT7, ZnT9, ZIP3, ZIP5, B 0 AT1, LAT1, y+LAT2, rBAT and PepT1 in the jejunum of broilers at 28 (A) and 39 d (B) of age. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B 0 AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y+LAT2, y+L-type amino acid transporter 2; rBAT, b 0 , + -type amino acid transporter; PepT1, peptide-transporter 1; ZnS, Zn sulfate; Zn-Prot M, Zn proteinate with moderate chelation strength (Q f = 51.6).

Article Snippet: The primary antibodies and dilution rates were as follows: ZnT1, ab214356, 1:1000 (Abcam, Cambridge, UK); ZnT4, BA3484, 1:1000 (Boster, Wuhan, China); Zn transporter 5 (ZnT5), 25604-1-AP, 1:1000 (Proteintech, Chicago); ZnT7, 13966-1-AP, 1:1000 (Proteintech, Chicago, IL); ZnT9, BS62531, 1:1000 (Bioworld, Minneapolis, MN); Zn transporter 10 ( ZnT10 ), PA5-49188, 1:1000 (Invitrogen, CA); ZIP3, ab254868, 1:500 (Abcam, Cambridge, UK); ZIP5, ab105194, 1:1000 (Abcam, Cambridge, UK); B 0 AT1, ab180516, 1:1000 (Abcam, Cambridge, UK); LAT1, DF8065, 1:500 (Affinity Biosciences, Melbourne, Australia); y+LAT2, 13823-1-AP, 1:1000 (Proteintech, Chicago); rBAT, DF7379, 1:1000 (Affinity Biosciences, Melbourne, Australia) and PepT1, A03672-1, 1:500 (Boster, Wuhan, China).

Techniques: Western Blot

Journal: Poultry Science

Article Title: The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers

doi: 10.1016/j.psj.2023.102477

Figure Lengend Snippet: Effect of dietary Zn source on the protein expression levels of Zn, amino acid and small peptide transporters in the ileum of broilers at 28 and 39 d of age. 1

Article Snippet: The primary antibodies and dilution rates were as follows: ZnT1, ab214356, 1:1000 (Abcam, Cambridge, UK); ZnT4, BA3484, 1:1000 (Boster, Wuhan, China); Zn transporter 5 (ZnT5), 25604-1-AP, 1:1000 (Proteintech, Chicago); ZnT7, 13966-1-AP, 1:1000 (Proteintech, Chicago, IL); ZnT9, BS62531, 1:1000 (Bioworld, Minneapolis, MN); Zn transporter 10 ( ZnT10 ), PA5-49188, 1:1000 (Invitrogen, CA); ZIP3, ab254868, 1:500 (Abcam, Cambridge, UK); ZIP5, ab105194, 1:1000 (Abcam, Cambridge, UK); B 0 AT1, ab180516, 1:1000 (Abcam, Cambridge, UK); LAT1, DF8065, 1:500 (Affinity Biosciences, Melbourne, Australia); y+LAT2, 13823-1-AP, 1:1000 (Proteintech, Chicago); rBAT, DF7379, 1:1000 (Affinity Biosciences, Melbourne, Australia) and PepT1, A03672-1, 1:500 (Boster, Wuhan, China).

Techniques: Expressing, Control

Journal: Poultry Science

Article Title: The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers

doi: 10.1016/j.psj.2023.102477

Figure Lengend Snippet: Representative immunoblots of ZnT1, ZnT4, ZnT5, ZnT7, ZnT9, ZIP3, ZIP5, B 0 AT1, LAT1, y+LAT2, rBAT and PepT1 in the ileum of broilers at 28 (A) and 39 d (B) of age. ZnT1, zinc transporter 1; ZnT4, zinc transporter 4; ZnT5, zinc transporter 5; ZnT7, zinc transporter 7; ZnT9, zinc transporter 9; ZIP3, Zrt-irt-like protein 3; ZIP5, Zrt-irt-like protein 5; B 0 AT1, B-0-system neutral amino acid co-transporter; LAT1, L-type amino acid transporter 1; y+LAT2, y+L-type amino acid transporter 2; rBAT, b 0 , + -type amino acid transporter; PepT1, peptide-transporter 1; ZnS, Zn sulfate; Zn-Prot M, Zn proteinate with moderate chelation strength (Q f = 51.6).

Article Snippet: The primary antibodies and dilution rates were as follows: ZnT1, ab214356, 1:1000 (Abcam, Cambridge, UK); ZnT4, BA3484, 1:1000 (Boster, Wuhan, China); Zn transporter 5 (ZnT5), 25604-1-AP, 1:1000 (Proteintech, Chicago); ZnT7, 13966-1-AP, 1:1000 (Proteintech, Chicago, IL); ZnT9, BS62531, 1:1000 (Bioworld, Minneapolis, MN); Zn transporter 10 ( ZnT10 ), PA5-49188, 1:1000 (Invitrogen, CA); ZIP3, ab254868, 1:500 (Abcam, Cambridge, UK); ZIP5, ab105194, 1:1000 (Abcam, Cambridge, UK); B 0 AT1, ab180516, 1:1000 (Abcam, Cambridge, UK); LAT1, DF8065, 1:500 (Affinity Biosciences, Melbourne, Australia); y+LAT2, 13823-1-AP, 1:1000 (Proteintech, Chicago); rBAT, DF7379, 1:1000 (Affinity Biosciences, Melbourne, Australia) and PepT1, A03672-1, 1:500 (Boster, Wuhan, China).

Techniques: Western Blot