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pen84 plasmid  (Addgene inc)


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    Structured Review

    Addgene inc pen84 plasmid
    Pen84 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pen84/pEN84+-+CTCF-AID%5B71-114%5D-eGFP-FRT-Puro-FRT+targeting+construct+(Plasmid+%2386230)/pmc12207409-45-19-21
    Average 92 stars, based on 10 article reviews
    pen84 plasmid - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Construct:

    Article Title: Stepwise de novo establishment of inactive X chromosome architecture in early development.
    Article Snippet: X chromosome inactivation triggers a dramatic reprogramming of transcription and chromosome architecture.. However, how the chromatin organization of inactive X chromosome is established de novo in vivo remains elusive.. Here, we identified an Xist-separated megadomain structure (X-megadomains) on the inactive X chromosome in mouse extraembryonic lineages and extraembryonic endoderm (XEN) cell lines, and transiently in the embryonic lineages, before Dxz4-delineated megadomain formation at later stages in a strain-specific manner.

    Article Title: Stepwise de novo establishment of inactive X chromosome architecture in early development
    Article Snippet: .. Donor plasmids targeting either Rad21 or Ctcf gene were constructed based on plasmid pEN84-CTCF-AID-eGFP-FRT-Puro-FRT plasmid (Addgene, 86230). ..

    Article Title: Stepwise de novo establishment of inactive X chromosome architecture in early development
    Article Snippet: .. To target Ctcf , the same homology arms from plasmid pEN 84-CTCF-AID-eGFP-FRT-Puro-FRT (Addgene, cat. no. 86230) and sgRNA were used as described 72 . pX330-U6-Chimeric_BB-CBh-hSpCas9-PuroR plasmid was used to construct CRISPR–Cas9 vectors. ..

    Article Title: DEAD-box RNA helicase 10 is required for 18S rRNA maturation by controlling the release of U3 snoRNA from pre-rRNA in embryonic stem cells.
    Article Snippet: .. To construct the Ddx10 donor plasmid, the AID-eGFP tag was PCR amplified from CTCF-AID-eGFP targeting vector (Addgene, 86230)51. ..

    Article Title: DEAD-box RNA helicase 10 is required for 18S rRNA maturation by controlling the release of U3 snoRNA from pre-rRNA in embryonic stem cells
    Article Snippet: .. To construct the Ddx10 donor plasmid, the AID-eGFP tag was PCR amplified from CTCF-AID-eGFP targeting vector (Addgene, 86230) . ..

    Article Title: Extrusion fountains are restricted by WAPL-dependent cohesin release and CTCF barriers.
    Article Snippet: .. The donor plasmid used to target the endogenous mouse CTCF and RAD21 protein was constructed by modifying a published pEN84 plasmid (Addgene #86 230). ..

    Article Title: Extrusion fountains are restricted by WAPL-dependent cohesin release and CTCF barriers
    Article Snippet: .. The donor plasmid used to target the endogenous mouse CTCF and RAD21 protein was constructed by modifying a published pEN84 plasmid (Addgene #86 230). ..

    Plasmid Preparation:

    Article Title: Stepwise de novo establishment of inactive X chromosome architecture in early development.
    Article Snippet: X chromosome inactivation triggers a dramatic reprogramming of transcription and chromosome architecture.. However, how the chromatin organization of inactive X chromosome is established de novo in vivo remains elusive.. Here, we identified an Xist-separated megadomain structure (X-megadomains) on the inactive X chromosome in mouse extraembryonic lineages and extraembryonic endoderm (XEN) cell lines, and transiently in the embryonic lineages, before Dxz4-delineated megadomain formation at later stages in a strain-specific manner.

    Article Title: Stepwise de novo establishment of inactive X chromosome architecture in early development
    Article Snippet: .. Donor plasmids targeting either Rad21 or Ctcf gene were constructed based on plasmid pEN84-CTCF-AID-eGFP-FRT-Puro-FRT plasmid (Addgene, 86230). ..

    Article Title: Stepwise de novo establishment of inactive X chromosome architecture in early development
    Article Snippet: .. To target Ctcf , the same homology arms from plasmid pEN 84-CTCF-AID-eGFP-FRT-Puro-FRT (Addgene, cat. no. 86230) and sgRNA were used as described 72 . pX330-U6-Chimeric_BB-CBh-hSpCas9-PuroR plasmid was used to construct CRISPR–Cas9 vectors. ..

    Article Title: DEAD-box RNA helicase 10 is required for 18S rRNA maturation by controlling the release of U3 snoRNA from pre-rRNA in embryonic stem cells.
    Article Snippet: .. To construct the Ddx10 donor plasmid, the AID-eGFP tag was PCR amplified from CTCF-AID-eGFP targeting vector (Addgene, 86230)51. ..

    Article Title: DEAD-box RNA helicase 10 is required for 18S rRNA maturation by controlling the release of U3 snoRNA from pre-rRNA in embryonic stem cells
    Article Snippet: .. To construct the Ddx10 donor plasmid, the AID-eGFP tag was PCR amplified from CTCF-AID-eGFP targeting vector (Addgene, 86230) . ..

    Article Title: Extrusion fountains are restricted by WAPL-dependent cohesin release and CTCF barriers.
    Article Snippet: .. The donor plasmid used to target the endogenous mouse CTCF and RAD21 protein was constructed by modifying a published pEN84 plasmid (Addgene #86 230). ..

    Article Title: Extrusion fountains are restricted by WAPL-dependent cohesin release and CTCF barriers
    Article Snippet: .. The donor plasmid used to target the endogenous mouse CTCF and RAD21 protein was constructed by modifying a published pEN84 plasmid (Addgene #86 230). ..

    CRISPR:

    Article Title: Stepwise de novo establishment of inactive X chromosome architecture in early development
    Article Snippet: .. To target Ctcf , the same homology arms from plasmid pEN 84-CTCF-AID-eGFP-FRT-Puro-FRT (Addgene, cat. no. 86230) and sgRNA were used as described 72 . pX330-U6-Chimeric_BB-CBh-hSpCas9-PuroR plasmid was used to construct CRISPR–Cas9 vectors. ..

    Article Title: Stepwise de novo establishment of inactive X chromosome architecture in early development.
    Article Snippet: X chromosome inactivation triggers a dramatic reprogramming of transcription and chromosome architecture.. However, how the chromatin organization of inactive X chromosome is established de novo in vivo remains elusive.. Here, we identified an Xist-separated megadomain structure (X-megadomains) on the inactive X chromosome in mouse extraembryonic lineages and extraembryonic endoderm (XEN) cell lines, and transiently in the embryonic lineages, before Dxz4-delineated megadomain formation at later stages in a strain-specific manner.

    Polymerase Chain Reaction:

    Article Title: DEAD-box RNA helicase 10 is required for 18S rRNA maturation by controlling the release of U3 snoRNA from pre-rRNA in embryonic stem cells.
    Article Snippet: .. To construct the Ddx10 donor plasmid, the AID-eGFP tag was PCR amplified from CTCF-AID-eGFP targeting vector (Addgene, 86230)51. ..

    Article Title: DEAD-box RNA helicase 10 is required for 18S rRNA maturation by controlling the release of U3 snoRNA from pre-rRNA in embryonic stem cells
    Article Snippet: .. To construct the Ddx10 donor plasmid, the AID-eGFP tag was PCR amplified from CTCF-AID-eGFP targeting vector (Addgene, 86230) . ..

    Amplification:

    Article Title: DEAD-box RNA helicase 10 is required for 18S rRNA maturation by controlling the release of U3 snoRNA from pre-rRNA in embryonic stem cells.
    Article Snippet: .. To construct the Ddx10 donor plasmid, the AID-eGFP tag was PCR amplified from CTCF-AID-eGFP targeting vector (Addgene, 86230)51. ..

    Article Title: DEAD-box RNA helicase 10 is required for 18S rRNA maturation by controlling the release of U3 snoRNA from pre-rRNA in embryonic stem cells
    Article Snippet: .. To construct the Ddx10 donor plasmid, the AID-eGFP tag was PCR amplified from CTCF-AID-eGFP targeting vector (Addgene, 86230) . ..



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