pegfp c1 (TaKaRa)
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pegfp c1
Pegfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+c1/pmCherry-C1+Vector/bio_rxiv__64898__2026__03__03__709279-248-18-23
Average 96 stars, based on 3287 article reviews
Pegfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+c1/pmCherry-C1+Vector/bio_rxiv__64898__2026__03__03__709279-248-18-23
Average 96 stars, based on 3287 article reviews
pegfp c1 - by Bioz Stars,
2026-10
96/100 stars
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Expressing:Article Title: Membrane Curvature Activates Src kinase and Promotes Metastatic Cancer Cell Survival Article Snippet: .. These DNA fragments were used to replace the EGFP in pEGFP-N1 for mammalian expression, or inserted into pEGFP-N1, Plasmid Preparation:Article Title: Membrane Curvature Activates Src kinase and Promotes Metastatic Cancer Cell Survival Article Snippet: .. These DNA fragments were used to replace the EGFP in pEGFP-N1 for mammalian expression, or inserted into pEGFP-N1, Article Title: Bacteria based protein delivery Article Snippet: .. Further at the 3′ end of YopE1-138 the following cleavage sites were added: XbaI-XhoI-BstBI-(HindIII) (FIG. 3 B). pBad_Si1 is equal to pBad_Si2 but encodes EGFP amplified from Construct:Article Title: Membrane Curvature Activates Src kinase and Promotes Metastatic Cancer Cell Survival Article Snippet: .. These DNA fragments were used to replace the EGFP in pEGFP-N1 for mammalian expression, or inserted into pEGFP-N1, Amplification:Article Title: Bacteria based protein delivery Article Snippet: Alternatively, if a vector for chromosomal or virulence plasmid insertion or a polynucleic acid construct such as e.g. a nucleotide sequence for chromosomal or virulence plasmid insertion is used, an endogenous promoter and the delivery signal from a bacterial effector protein can be encoded on the endogenous bacterial DNA (chromosomal or plasmid DNA) and only the polynucleic acid construct such as e.g. a nucleotide sequence encoding the heterologous protein will be provided by a vector for chromosomal or virulence plasmid insertion or by a polynucleic acid construct such as e.g. a nucleotide sequence for chromosomal or virulence plasmid insertion. .. Further at the 3′ end of YopE1-138 the following cleavage sites were added: XbaI-XhoI-BstBI-(HindIII). pBad_Si1 is equal to pBad_Si2 but encodes EGFP amplified from Article Title: Bacteria based protein delivery Article Snippet: .. Further at the 3′ end of YopE1-138 the following cleavage sites were added: XbaI-XhoI-BstBI-(HindIII) (FIG. 3 B). pBad_Si1 is equal to pBad_Si2 but encodes EGFP amplified from other:Article Title: A luminescence-based biosensor to measure endogenous UBE3A activity Article Snippet: Wildtype (WT) C57BL/6J mice (#000664), Ube3a knock-out mice and UBE3A-YFP knockin mice were purchased from the Jackson Laboratory and maintained in the lab. Ube3a T503A mice were generated in the lab as previously described. Synthesized:Article Title: Bacteria based protein delivery Article Snippet: .. Further at the 3′ end of YopE1-138 the following cleavage sites were added: XbaI-XhoI-BstBI-(HindIII) (FIG. 3 B). pBad_Si1 is equal to pBad_Si2 but encodes EGFP amplified from Sequencing:Article Title: Functional assessment of protein variants in structured domains by fluorescence cross-correlation spectroscopy. Article Snippet: AR TIC LE IN PR ES S 129 MATERIALS AND METHODS 130 131 Cell lines 132 HeLa cells (ATCC® CCL-2TM) were cultured in Dulbecco’s Modified Eagle Medium 133 (DMEM), high glucose, no glutamine, no phenol red (Thermo Fisher Scientific, Cat. No. 134 31053028), supplemented with 1X GlutaMAXTM (Thermo Fisher Scientific, Cat. No. 135 35050038), 1 mM sodium pyruvate (Thermo Fisher Scientific, Cat. No. 11360039), 10% 136 FBS (Biowest, Cat. No. BWSTS1810) and 1% penicillin/streptomycin (Thermo Fisher 137 Scientific, Cat. No. 15070063) and were grown in a humidified incubator at 37oC and 5% 138 CO2. .. 139 140 Plasmids 141 The coding sequence of BRCA1 was PCR-amplified and cloned into Article Title: Functional assessment of protein variants in structured domains by fluorescence cross-correlation spectroscopy Article Snippet: HeLa cells (ATCC ® CCL-2TM) were cultured in Dulbecco’s Modified Eagle Medium (DMEM), high glucose, no glutamine, no phenol red (Thermo Fisher Scientific, Cat. No. 31053028), supplemented with 1X GlutaMAXTM (Thermo Fisher Scientific, Cat. No. 35050038), 1 mM sodium pyruvate (Thermo Fisher Scientific, Cat. No. 11360039), 10% FBS (Biowest, Cat. No. BWSTS1810) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Cat. No. 15070063) and were grown in a humidified incubator at 37 °C and 5% CO 2 . .. The coding sequence of BRCA1 was PCR-amplified and cloned into Polymerase Chain Reaction:Article Title: Functional assessment of protein variants in structured domains by fluorescence cross-correlation spectroscopy. Article Snippet: AR TIC LE IN PR ES S 129 MATERIALS AND METHODS 130 131 Cell lines 132 HeLa cells (ATCC® CCL-2TM) were cultured in Dulbecco’s Modified Eagle Medium 133 (DMEM), high glucose, no glutamine, no phenol red (Thermo Fisher Scientific, Cat. No. 134 31053028), supplemented with 1X GlutaMAXTM (Thermo Fisher Scientific, Cat. No. 135 35050038), 1 mM sodium pyruvate (Thermo Fisher Scientific, Cat. No. 11360039), 10% 136 FBS (Biowest, Cat. No. BWSTS1810) and 1% penicillin/streptomycin (Thermo Fisher 137 Scientific, Cat. No. 15070063) and were grown in a humidified incubator at 37oC and 5% 138 CO2. .. 139 140 Plasmids 141 The coding sequence of BRCA1 was PCR-amplified and cloned into Article Title: Functional assessment of protein variants in structured domains by fluorescence cross-correlation spectroscopy Article Snippet: HeLa cells (ATCC ® CCL-2TM) were cultured in Dulbecco’s Modified Eagle Medium (DMEM), high glucose, no glutamine, no phenol red (Thermo Fisher Scientific, Cat. No. 31053028), supplemented with 1X GlutaMAXTM (Thermo Fisher Scientific, Cat. No. 35050038), 1 mM sodium pyruvate (Thermo Fisher Scientific, Cat. No. 11360039), 10% FBS (Biowest, Cat. No. BWSTS1810) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Cat. No. 15070063) and were grown in a humidified incubator at 37 °C and 5% CO 2 . .. The coding sequence of BRCA1 was PCR-amplified and cloned into Clone Assay:Article Title: Functional assessment of protein variants in structured domains by fluorescence cross-correlation spectroscopy. Article Snippet: AR TIC LE IN PR ES S 129 MATERIALS AND METHODS 130 131 Cell lines 132 HeLa cells (ATCC® CCL-2TM) were cultured in Dulbecco’s Modified Eagle Medium 133 (DMEM), high glucose, no glutamine, no phenol red (Thermo Fisher Scientific, Cat. No. 134 31053028), supplemented with 1X GlutaMAXTM (Thermo Fisher Scientific, Cat. No. 135 35050038), 1 mM sodium pyruvate (Thermo Fisher Scientific, Cat. No. 11360039), 10% 136 FBS (Biowest, Cat. No. BWSTS1810) and 1% penicillin/streptomycin (Thermo Fisher 137 Scientific, Cat. No. 15070063) and were grown in a humidified incubator at 37oC and 5% 138 CO2. .. 139 140 Plasmids 141 The coding sequence of BRCA1 was PCR-amplified and cloned into Article Title: Functional assessment of protein variants in structured domains by fluorescence cross-correlation spectroscopy Article Snippet: HeLa cells (ATCC ® CCL-2TM) were cultured in Dulbecco’s Modified Eagle Medium (DMEM), high glucose, no glutamine, no phenol red (Thermo Fisher Scientific, Cat. No. 31053028), supplemented with 1X GlutaMAXTM (Thermo Fisher Scientific, Cat. No. 35050038), 1 mM sodium pyruvate (Thermo Fisher Scientific, Cat. No. 11360039), 10% FBS (Biowest, Cat. No. BWSTS1810) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Cat. No. 15070063) and were grown in a humidified incubator at 37 °C and 5% CO 2 . .. The coding sequence of BRCA1 was PCR-amplified and cloned into Article Title: Talin–tensin3 interactions regulate fibrillar adhesion formation and tensin3 phase separation Article Snippet: .. To generate the mitochondria targeting GFP-cBAK and mCh-cBAK vectors, the cBAK fragment (5′-TTG CGT AGA GAC CCC ATC CTG ACC GTA ATG GTG ATT TTT GGT GTG GTT CTG TTG GGC CAA TTC GTG GTA CAC AGA TTC TTC AGA TCA TGA-3′) was cloned into the Ligation:Article Title: Functional assessment of protein variants in structured domains by fluorescence cross-correlation spectroscopy. Article Snippet: AR TIC LE IN PR ES S 129 MATERIALS AND METHODS 130 131 Cell lines 132 HeLa cells (ATCC® CCL-2TM) were cultured in Dulbecco’s Modified Eagle Medium 133 (DMEM), high glucose, no glutamine, no phenol red (Thermo Fisher Scientific, Cat. No. 134 31053028), supplemented with 1X GlutaMAXTM (Thermo Fisher Scientific, Cat. No. 135 35050038), 1 mM sodium pyruvate (Thermo Fisher Scientific, Cat. No. 11360039), 10% 136 FBS (Biowest, Cat. No. BWSTS1810) and 1% penicillin/streptomycin (Thermo Fisher 137 Scientific, Cat. No. 15070063) and were grown in a humidified incubator at 37oC and 5% 138 CO2. .. 139 140 Plasmids 141 The coding sequence of BRCA1 was PCR-amplified and cloned into Article Title: Functional assessment of protein variants in structured domains by fluorescence cross-correlation spectroscopy Article Snippet: HeLa cells (ATCC ® CCL-2TM) were cultured in Dulbecco’s Modified Eagle Medium (DMEM), high glucose, no glutamine, no phenol red (Thermo Fisher Scientific, Cat. No. 31053028), supplemented with 1X GlutaMAXTM (Thermo Fisher Scientific, Cat. No. 35050038), 1 mM sodium pyruvate (Thermo Fisher Scientific, Cat. No. 11360039), 10% FBS (Biowest, Cat. No. BWSTS1810) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Cat. No. 15070063) and were grown in a humidified incubator at 37 °C and 5% CO 2 . .. The coding sequence of BRCA1 was PCR-amplified and cloned into Countercurrent Chromatography:Article Title: Talin–tensin3 interactions regulate fibrillar adhesion formation and tensin3 phase separation Article Snippet: .. To generate the mitochondria targeting GFP-cBAK and mCh-cBAK vectors, the cBAK fragment (5′-TTG CGT AGA GAC CCC ATC CTG ACC GTA ATG GTG ATT TTT GGT GTG GTT CTG TTG GGC CAA TTC GTG GTA CAC AGA TTC TTC AGA TCA TGA-3′) was cloned into the |
