pdd122 (Addgene inc)
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pdd122
Pdd122, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdd122/pDD122+(Peft-3%3A%3ACas9+%2B+ttTi5605+sgRNA)+(Plasmid+%2347550)/bio_rxiv__2025__05__02__651978-245-14-15
Average 92 stars, based on 19 article reviews
Pdd122, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdd122/pDD122+(Peft-3%3A%3ACas9+%2B+ttTi5605+sgRNA)+(Plasmid+%2347550)/bio_rxiv__2025__05__02__651978-245-14-15
Average 92 stars, based on 19 article reviews
pdd122 - by Bioz Stars,
2026-09
92/100 stars
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Plasmid Preparation:Article Title: Evolutionary conserved regulation of TFEB stability by the E3 ubiquitin ligase WWP2 modulates response to stress in vivo Article Snippet: .. The strain was maintained using 50 μg / ml hygromycine B to select animals with extrachromosomal array. Article Title: Specialized high-capacity mitochondria fuel cell invasion Article Snippet: PureLinkTM HiPure Plasmid Miniprep (Invitrogen #K210002) was used to isolate high purity sgRNA plasmid DNA and SEC repair plasmid DNA for injection. https://crispor.gi.ucsc.edu/ was used to identify short guide sequences where the fluorescent protein should be inserted and PAM sites where the Cas9 protein cleaves. .. The short guide RNA (sgRNA) plasmid was generated by cutting the Cas9 guide plasmid, Article Title: Optimized PAR-2 RING dimerization mediates cooperative and selective membrane binding for robust cell polarity Article Snippet: pRI021 (ttTi5605 Mos1 insertion vector (mex-5 promoter and nmy-2 3' UTR)) , This study , . .. Article Title: P5A ATPase controls ER translocation of Wnt in neuronal migration. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-FLAG Sigma Cat# F1804; RRID: AB_262044 Mouse anti-GFP Abmart Cat# M20004; RRID: AB_2619674 Rabbit anti-ATP13A1 Proteintech Cat# 16244-1-AP; RRID:AB_10015282 Donker anti-Rabbit Jackson Immunoresearch Cat# 711-035-152; RRID: AB_10015282 Rabbit anti-Mouse Sigma Cat# A9044; RRID: AB_258431 Rabbit anti-b-Actin Marine Biological Laboratory Cat# PM053-7; RRID: AB_10697035 Monoclonal ANTI-FLAG M2 Affinity Gel Sigma Cat# A2220; RRID: AB_10063035 Bacterial and virus strains OP50 CGC https://www.cgc.edu/strain/OP50 Chemicals, peptides, and recombinant proteins KOD-Plus-Neo TOYOBO Cat# KOD-401 KOD OneTM PCR Master Mix TOYOBO Cat# KMM-101 Experimental models: cell lines .. Experimental models: organisms/strains C. elegans strain, see Table S1 This paper N/A Oligonucleotides See Table S2 This paper N/A Recombinant DNA Plasmid: Article Title: Meisosomes, folded membrane microdomains between the apical extracellular matrix and epidermis Article Snippet: The transgene frSi26 is a single-copy insertion on chromosome II (ttTi5605 location) of pNP165 ( dpy-7 p::VHA-5::GFP) by CRISPR using a self-excising cassette (SEC) ( ). pNP165 was obtained by insertion of the dpy-7 promoter, which leads to an epidermal specific expression, in front of VHA-5::GFP into the pNP154 vector. pNP154 was made from a vector containing the SEC cassette for single insertion on chromosome II at the position of ttTi5605 (pAP087, kindly provided by Ari Pani) ( ). .. Constructs were designed using the plasmid editor Ape ( ) and made using Gibson Assembly (NEB Inc, MA) and confirmed by sequencing. pNP165 was injected in N2 at 20 ng/μl together with Article Title: Reciprocal discoidin domain receptor signaling strengthens integrin adhesion to connect adjacent tissues Article Snippet: Recombinant DNA reagent , Plasmid: pCFJ352 , Addgene , RRID: Addgene_30539 , sgRNA targeting MosSCI insertion site on Chr I, from Erik Jorgensen. .. Recombinant DNA reagent , Plasmid: Article Title: A polarity pathway for exocyst-dependent intracellular tube extension Article Snippet: .. Recombinant DNA reagent , Peft-3::Cas9 + ttTi5605 sgRNA , , Generated:Article Title: Specialized high-capacity mitochondria fuel cell invasion Article Snippet: PureLinkTM HiPure Plasmid Miniprep (Invitrogen #K210002) was used to isolate high purity sgRNA plasmid DNA and SEC repair plasmid DNA for injection. https://crispor.gi.ucsc.edu/ was used to identify short guide sequences where the fluorescent protein should be inserted and PAM sites where the Cas9 protein cleaves. .. The short guide RNA (sgRNA) plasmid was generated by cutting the Cas9 guide plasmid, Recombinant:Article Title: P5A ATPase controls ER translocation of Wnt in neuronal migration. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-FLAG Sigma Cat# F1804; RRID: AB_262044 Mouse anti-GFP Abmart Cat# M20004; RRID: AB_2619674 Rabbit anti-ATP13A1 Proteintech Cat# 16244-1-AP; RRID:AB_10015282 Donker anti-Rabbit Jackson Immunoresearch Cat# 711-035-152; RRID: AB_10015282 Rabbit anti-Mouse Sigma Cat# A9044; RRID: AB_258431 Rabbit anti-b-Actin Marine Biological Laboratory Cat# PM053-7; RRID: AB_10697035 Monoclonal ANTI-FLAG M2 Affinity Gel Sigma Cat# A2220; RRID: AB_10063035 Bacterial and virus strains OP50 CGC https://www.cgc.edu/strain/OP50 Chemicals, peptides, and recombinant proteins KOD-Plus-Neo TOYOBO Cat# KOD-401 KOD OneTM PCR Master Mix TOYOBO Cat# KMM-101 Experimental models: cell lines .. Experimental models: organisms/strains C. elegans strain, see Table S1 This paper N/A Oligonucleotides See Table S2 This paper N/A Recombinant DNA Plasmid: Article Title: Reciprocal discoidin domain receptor signaling strengthens integrin adhesion to connect adjacent tissues Article Snippet: Recombinant DNA reagent , Plasmid: pCFJ352 , Addgene , RRID: Addgene_30539 , sgRNA targeting MosSCI insertion site on Chr I, from Erik Jorgensen. .. Recombinant DNA reagent , Plasmid: Article Title: A polarity pathway for exocyst-dependent intracellular tube extension Article Snippet: .. Recombinant DNA reagent , Peft-3::Cas9 + ttTi5605 sgRNA , , Construct:Article Title: Meisosomes, folded membrane microdomains between the apical extracellular matrix and epidermis Article Snippet: The transgene frSi26 is a single-copy insertion on chromosome II (ttTi5605 location) of pNP165 ( dpy-7 p::VHA-5::GFP) by CRISPR using a self-excising cassette (SEC) ( ). pNP165 was obtained by insertion of the dpy-7 promoter, which leads to an epidermal specific expression, in front of VHA-5::GFP into the pNP154 vector. pNP154 was made from a vector containing the SEC cassette for single insertion on chromosome II at the position of ttTi5605 (pAP087, kindly provided by Ari Pani) ( ). .. Constructs were designed using the plasmid editor Ape ( ) and made using Gibson Assembly (NEB Inc, MA) and confirmed by sequencing. pNP165 was injected in N2 at 20 ng/μl together with Sequencing:Article Title: Meisosomes, folded membrane microdomains between the apical extracellular matrix and epidermis Article Snippet: The transgene frSi26 is a single-copy insertion on chromosome II (ttTi5605 location) of pNP165 ( dpy-7 p::VHA-5::GFP) by CRISPR using a self-excising cassette (SEC) ( ). pNP165 was obtained by insertion of the dpy-7 promoter, which leads to an epidermal specific expression, in front of VHA-5::GFP into the pNP154 vector. pNP154 was made from a vector containing the SEC cassette for single insertion on chromosome II at the position of ttTi5605 (pAP087, kindly provided by Ari Pani) ( ). .. Constructs were designed using the plasmid editor Ape ( ) and made using Gibson Assembly (NEB Inc, MA) and confirmed by sequencing. pNP165 was injected in N2 at 20 ng/μl together with Injection:Article Title: Meisosomes, folded membrane microdomains between the apical extracellular matrix and epidermis Article Snippet: The transgene frSi26 is a single-copy insertion on chromosome II (ttTi5605 location) of pNP165 ( dpy-7 p::VHA-5::GFP) by CRISPR using a self-excising cassette (SEC) ( ). pNP165 was obtained by insertion of the dpy-7 promoter, which leads to an epidermal specific expression, in front of VHA-5::GFP into the pNP154 vector. pNP154 was made from a vector containing the SEC cassette for single insertion on chromosome II at the position of ttTi5605 (pAP087, kindly provided by Ari Pani) ( ). .. Constructs were designed using the plasmid editor Ape ( ) and made using Gibson Assembly (NEB Inc, MA) and confirmed by sequencing. pNP165 was injected in N2 at 20 ng/μl together with |
