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pd98059  (Tocris)


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    Tocris pd98059
    Pd98059, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 848 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pd+98059/PD+98059/pmc13018835-175-37-38
    Average 96 stars, based on 848 article reviews
    pd98059 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Modeling neurodevelopment and disease using human pluripotent stem cells and mouse models
    Article Snippet: These sequences were individually cloned into plasmid pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene #62988) and then electroporated into the mESCs using the Lonza Nucleofector 2b Device and Cell Nucleofector Kit (Lonza #VAPH1001). .. The mESCs were cultured in feeder-free 2i media (DMEM/F-12 (Gibco #11320033) and Neurobasal Medium (Gibco #21103049) (prepared at a 1:1 ratio) supplemented with N2 supplement (Gibco #17502048), B27 serum-free supplement (Gibco #17504044), penicillin/streptomycin (Gibco #15140122), GlutaMAX (Gibco #35050061), bovine serum albumin (Thermo Scientific Chemicals #AAJ64248AE), 55 μM 2-mercaptoethanol (Gibco #21985023), 3μM CHIR99021 (Axon #1386), 1μM PD 98059 (Tocris Bioscience #1213), and ESGRO recombinant mouse LIF protein (1000 units/ml, MilliporeSigma #ESG1107). .. Antibiotic selection was initiated 24 hours after nucleofection in 2i media containing 1.5 μg/ml puromycin (Fisher BioReagents #BP2956100) for about 72 hours.

    Recombinant:

    Article Title: Modeling neurodevelopment and disease using human pluripotent stem cells and mouse models
    Article Snippet: These sequences were individually cloned into plasmid pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene #62988) and then electroporated into the mESCs using the Lonza Nucleofector 2b Device and Cell Nucleofector Kit (Lonza #VAPH1001). .. The mESCs were cultured in feeder-free 2i media (DMEM/F-12 (Gibco #11320033) and Neurobasal Medium (Gibco #21103049) (prepared at a 1:1 ratio) supplemented with N2 supplement (Gibco #17502048), B27 serum-free supplement (Gibco #17504044), penicillin/streptomycin (Gibco #15140122), GlutaMAX (Gibco #35050061), bovine serum albumin (Thermo Scientific Chemicals #AAJ64248AE), 55 μM 2-mercaptoethanol (Gibco #21985023), 3μM CHIR99021 (Axon #1386), 1μM PD 98059 (Tocris Bioscience #1213), and ESGRO recombinant mouse LIF protein (1000 units/ml, MilliporeSigma #ESG1107). .. Antibiotic selection was initiated 24 hours after nucleofection in 2i media containing 1.5 μg/ml puromycin (Fisher BioReagents #BP2956100) for about 72 hours.

    other:

    Article Title: Numb positively regulates Hedgehog signaling at the ciliary pocket
    Article Snippet: Antibiotic selection was initiated 24 h after nucleofection in 2i media containing 1.5 μg/ml puromycin (Fisher BioReagents #BP2956100) for 72 h. Clones were picked individually, expanded, and genomic DNA was collected using QuickExtract DNA extraction solution (Lucigen, #QE09050).

    Article Title: Numb positively regulates Hedgehog signaling at the ciliary pocket.
    Article Snippet: Antibiotic selectionwas initiated 24 h after nucleofection in 2i media containing 1.5μg/ml puromycin (Fisher BioReagents #BP2956100) for 72 h. Clones were picked individually, expanded, and genomic DNA was collected using QuickExtract DNA extraction solution (Lucigen, #QE09050).

    Article Title: Mitochondrial integrity modulates mTOR signaling and podocyte function
    Article Snippet: PD 98059 , Tocris , 1213.



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    Inhibition of the NF-κB, p38 and JNK pathway components had an effect on the nucleic acid induced chemokine expression of NHEKs. Specific inhibiton of signaling pathway components was obtained by inhibitors (NF-κB (Bay 11−7085, 10 μM); STING (H151, 200 ng/ml); STAT-1 (Fludarabine, 10 μM); STAT-3 (Stattic, 5 μM); <t>MEK1</t> <t>(PD98059,</t> 20 μM); JNK (SP600125, 10 μM) and p38 (SB203580, 10 μM)) for 60 minutes before transfecting the cells with 0,666 μg/mL poly(I:C) or 1 ug/mL poly(dA:dT). Gene expression of fractalkine (A-C), CCL2 (D-F) and CXCL10 (G-I) was determined by the ∆∆Ct method, with GAPDH as a normalizing gene compared to the expression of the nontreated control samples. Data are presented as mean ± SD with individual datapoints, white squares representing poly(I:C), black triangles representing poly(dA:dT) treatment, n = 3. Gene expression differences were analyzed using linear mixed-effects models, p-values were adjusted using the Benjamini-Hochberg method within each solvent group-stimulus combination **** p < 0.0001, *** p < 0.001,** p < 0.01, *p < 0.05.
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    Inhibition of the NF-κB, p38 and JNK pathway components had an effect on the nucleic acid induced chemokine expression of NHEKs. Specific inhibiton of signaling pathway components was obtained by inhibitors (NF-κB (Bay 11−7085, 10 μM); STING (H151, 200 ng/ml); STAT-1 (Fludarabine, 10 μM); STAT-3 (Stattic, 5 μM); <t>MEK1</t> <t>(PD98059,</t> 20 μM); JNK (SP600125, 10 μM) and p38 (SB203580, 10 μM)) for 60 minutes before transfecting the cells with 0,666 μg/mL poly(I:C) or 1 ug/mL poly(dA:dT). Gene expression of fractalkine (A-C), CCL2 (D-F) and CXCL10 (G-I) was determined by the ∆∆Ct method, with GAPDH as a normalizing gene compared to the expression of the nontreated control samples. Data are presented as mean ± SD with individual datapoints, white squares representing poly(I:C), black triangles representing poly(dA:dT) treatment, n = 3. Gene expression differences were analyzed using linear mixed-effects models, p-values were adjusted using the Benjamini-Hochberg method within each solvent group-stimulus combination **** p < 0.0001, *** p < 0.001,** p < 0.01, *p < 0.05.
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    Image Search Results


    Inhibition of the NF-κB, p38 and JNK pathway components had an effect on the nucleic acid induced chemokine expression of NHEKs. Specific inhibiton of signaling pathway components was obtained by inhibitors (NF-κB (Bay 11−7085, 10 μM); STING (H151, 200 ng/ml); STAT-1 (Fludarabine, 10 μM); STAT-3 (Stattic, 5 μM); MEK1 (PD98059, 20 μM); JNK (SP600125, 10 μM) and p38 (SB203580, 10 μM)) for 60 minutes before transfecting the cells with 0,666 μg/mL poly(I:C) or 1 ug/mL poly(dA:dT). Gene expression of fractalkine (A-C), CCL2 (D-F) and CXCL10 (G-I) was determined by the ∆∆Ct method, with GAPDH as a normalizing gene compared to the expression of the nontreated control samples. Data are presented as mean ± SD with individual datapoints, white squares representing poly(I:C), black triangles representing poly(dA:dT) treatment, n = 3. Gene expression differences were analyzed using linear mixed-effects models, p-values were adjusted using the Benjamini-Hochberg method within each solvent group-stimulus combination **** p < 0.0001, *** p < 0.001,** p < 0.01, *p < 0.05.

    Journal: PLOS One

    Article Title: Nucleic acid-induced chemokine expression in keratinocytes: Implications for skin inflammation

    doi: 10.1371/journal.pone.0336901

    Figure Lengend Snippet: Inhibition of the NF-κB, p38 and JNK pathway components had an effect on the nucleic acid induced chemokine expression of NHEKs. Specific inhibiton of signaling pathway components was obtained by inhibitors (NF-κB (Bay 11−7085, 10 μM); STING (H151, 200 ng/ml); STAT-1 (Fludarabine, 10 μM); STAT-3 (Stattic, 5 μM); MEK1 (PD98059, 20 μM); JNK (SP600125, 10 μM) and p38 (SB203580, 10 μM)) for 60 minutes before transfecting the cells with 0,666 μg/mL poly(I:C) or 1 ug/mL poly(dA:dT). Gene expression of fractalkine (A-C), CCL2 (D-F) and CXCL10 (G-I) was determined by the ∆∆Ct method, with GAPDH as a normalizing gene compared to the expression of the nontreated control samples. Data are presented as mean ± SD with individual datapoints, white squares representing poly(I:C), black triangles representing poly(dA:dT) treatment, n = 3. Gene expression differences were analyzed using linear mixed-effects models, p-values were adjusted using the Benjamini-Hochberg method within each solvent group-stimulus combination **** p < 0.0001, *** p < 0.001,** p < 0.01, *p < 0.05.

    Article Snippet: For inhibition studies, cells were incubated for 60 minutes prior to poly(dA:dT)/poly(I:C) transfection with inhibitors for NF-κB (Bay 11−7085, 10 μM; MedChem Express, Monmouth Junction, NJ, USA), STING (H151, 200 ng/ml, InvivoGene) STAT-1 (Fludarabine, 10 μM; Sigma Aldrich), STAT-3 (Stattic, 5 μM; Sigma Aldrich), MEK1 (PD98059, 20 μM; Sigma Aldrich), JNK (SP600125, 10 μM; Tocris Bioscience, Bristol, UK) and p38 (SB203580, 10 μM; Tocris Bioscience).

    Techniques: Inhibition, Expressing, Gene Expression, Control, Solvent