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primary epidermal melanocytes; normal, human, adult  (ATCC)


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    ATCC primary epidermal melanocytes; normal, human, adult
    Primary Epidermal Melanocytes; Normal, Human, Adult, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcs+200+013/Primary+Epidermal+Melanocytes%3B+Normal%2C+Human%2C+Adult/custom%40pcs-200-013%4042068671
    Average 99 stars, based on 330 article reviews
    primary epidermal melanocytes; normal, human, adult - by Bioz Stars, 2026-10
    99/100 stars

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    other:

    Article Title: Inhibition of mitochondrial respiration prevents BRAF -mutant melanoma brain metastasis
    Article Snippet: PCS-200-013) were purchased from ATCC.

    Article Title: Exon skipping by peptide nucleic acid derivatives
    Article Snippet: Number PCS-200-013, ATCC) cells were maintained in Dermal Cell Basal Medium (Cat Number PCS-200-030, ATCC) supplemented with Adult Melanocyte Growth Kit Component (Cat.

    Article Title: Two novel atypical PKC inhibitors; ACPD and DNDA effectively mitigate cell proliferation and epithelial to mesenchymal transition of metastatic melanoma while inducing apoptosis
    Article Snippet: PCS-200-013, SK-MEL-2 and MeWo cell lines were purchased from the American Type Tissue Culture Collection (ATCC; Rockville, MD, USA) and MEL-F-NEO cell line was purchased from Zen-Bio, Inc. (Research Triangle Park, NC, USA).

    Article Title: TRPML1 Promotes Protein Homeostasis in Melanoma Cells by Negatively Regulating MAPK and mTORC1 Signaling
    Article Snippet: Adult human melanocytes , ATCC , Cat# PCS-200-013.

    Cell Culture:

    Article Title: MicroRNA-421 participates in vitiligo development through regulating human melanocyte survival by targeting receptor-interacting serine/threonine kinase 1
    Article Snippet: .. Primary epidermal melanocytes were obtained from the American Type Culture Collection (cat no. ATCC ® PCS-200-013) and cultured in Medium 254 (Gibco; Thermo Fisher Scientific, Inc.) supplemented with human melanocyte growth supplement (Gibco; Thermo Fisher Scientific, Inc.) at 37°C with 5% CO 2 . .. Primary epidermal melanocytes (1×10 6 cells per well) were transfected with the inhibitor control (5′-CAGUACUUUUGUGUAGUACAA-3′; Guangzhou Ribobio Co., Ltd.), miR-421 inhibitor (5′-GCGCCCAAUUAAUGUCUGUUGAU-3′; Guangzhou Ribobio Co., Ltd.), 0.2 μM control-shRNA (cat no. sc-108060; Santa Cruz Biotechnology, Inc.), 0.2 μM RIPK1-shRNA (cat no. sc-44326-SH; Santa Cruz Biotechnology, Inc.), miR-421 inhibitor + control-shRNA, or miR-421 inhibitor + RIPK1-shRNA for 24 h using Lipofectamine 2000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.



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    Role of four prognostically relevant genes. (A) Quantitative reverse transcription PCR for the calculated levels of four prognostically relevant genes in human epidermal <t>melanocytes.</t> (B) Validation of the knockdown efficiency of C2CD4B. (C, D) Scratch and Transwell assays exploring the migration and invasion of A375 cells in vitro. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, p > 0.05.
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    Role of four prognostically relevant genes. (A) Quantitative reverse transcription PCR for the calculated levels of four prognostically relevant genes in human epidermal melanocytes. (B) Validation of the knockdown efficiency of C2CD4B. (C, D) Scratch and Transwell assays exploring the migration and invasion of A375 cells in vitro. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, p > 0.05.

    Journal: Human Mutation

    Article Title: A Macrophage/Monocyte‐Related Four‐Gene Signature for Prognostic Assessment of Uveal Melanoma: BTBD6 , C2CD4B , CCL24 , and S100A4

    doi: 10.1155/humu/4978880

    Figure Lengend Snippet: Role of four prognostically relevant genes. (A) Quantitative reverse transcription PCR for the calculated levels of four prognostically relevant genes in human epidermal melanocytes. (B) Validation of the knockdown efficiency of C2CD4B. (C, D) Scratch and Transwell assays exploring the migration and invasion of A375 cells in vitro. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, p > 0.05.

    Article Snippet: Melanoma cell line A375 (CRL‐1619) and human epidermal melanocytes (PCS‐200‐013) were purchased from American Type Culture Collection (ATCC, Manassas, Virginia) and cultured as follows.

    Techniques: Reverse Transcription, Biomarker Discovery, Knockdown, Migration, In Vitro