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human pulmonary artery endothelial cells  (ATCC)


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    ATCC human pulmonary artery endothelial cells
    Human Pulmonary Artery Endothelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human pulmonary artery endothelial cells/product/ATCC
    Average 95 stars, based on 118 article reviews
    human pulmonary artery endothelial cells - by Bioz Stars, 2026-02
    95/100 stars

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    Effects of recombinant N‐protein (endotoxin nondepleted, NPnon) on inflammatory marker mRNA levels in human microvascular endothelial cells. Cells were treated with vehicle, LPS (1 μg/mL), or NPnon (10 μg/mL, ThermoFisher) for 4 h. The total RNA was isolated, and qPCR analysis was performed to measure the mRNA levels of TNFα, IL‐6, E‐selectin, and ICAM‐1. Data are presented as mean ± SEM and analyzed using one‐way ANOVA followed by Bonferroni's multiple comparisons test. n = 4–5 independent cell cultures per group. **** p < 0.0001 vs. control.

    Journal: The FASEB Journal

    Article Title: SARS ‐ CoV ‐2 Nucleocapsid Protein Does Not Induce Inflammation in Endothelial Cells or Monocytes

    doi: 10.1096/fj.202501433R

    Figure Lengend Snippet: Effects of recombinant N‐protein (endotoxin nondepleted, NPnon) on inflammatory marker mRNA levels in human microvascular endothelial cells. Cells were treated with vehicle, LPS (1 μg/mL), or NPnon (10 μg/mL, ThermoFisher) for 4 h. The total RNA was isolated, and qPCR analysis was performed to measure the mRNA levels of TNFα, IL‐6, E‐selectin, and ICAM‐1. Data are presented as mean ± SEM and analyzed using one‐way ANOVA followed by Bonferroni's multiple comparisons test. n = 4–5 independent cell cultures per group. **** p < 0.0001 vs. control.

    Article Snippet: Human pulmonary artery microvascular endothelial cells (HMEC, American Type Culture Collection (ATCC)) were cultured in endothelial cell media as described above, in an incubator at 37°C with 5% CO 2 .

    Techniques: Recombinant, Marker, Isolation, Control

    Effects of recombinant N‐protein (endotoxin nondepleted, NPnon) on inflammatory marker mRNA levels in mouse microvascular endothelial cells. Cells were treated with vehicle, LPS (1 μg/mL), NPnon (10 μg/mL, ThermoFisher), LPS + polymyxin B (PMB) (1.25 mg/mL), NPnon + PMB (250 μg/mL), or PMB (250 μg/mL) for 4 h. The total RNA was isolated and qPCR analysis was performed to measure the mRNA levels of TNFα, IL‐6, E‐selectin, and ICAM‐1. Data are presented as mean ± SEM and analyzed using one‐way ANOVA followed by Bonferroni's multiple comparisons test. n = 5 independent cell cultures per group. *** p < 0.001, ** p < 0.01 vs. control.

    Journal: The FASEB Journal

    Article Title: SARS ‐ CoV ‐2 Nucleocapsid Protein Does Not Induce Inflammation in Endothelial Cells or Monocytes

    doi: 10.1096/fj.202501433R

    Figure Lengend Snippet: Effects of recombinant N‐protein (endotoxin nondepleted, NPnon) on inflammatory marker mRNA levels in mouse microvascular endothelial cells. Cells were treated with vehicle, LPS (1 μg/mL), NPnon (10 μg/mL, ThermoFisher), LPS + polymyxin B (PMB) (1.25 mg/mL), NPnon + PMB (250 μg/mL), or PMB (250 μg/mL) for 4 h. The total RNA was isolated and qPCR analysis was performed to measure the mRNA levels of TNFα, IL‐6, E‐selectin, and ICAM‐1. Data are presented as mean ± SEM and analyzed using one‐way ANOVA followed by Bonferroni's multiple comparisons test. n = 5 independent cell cultures per group. *** p < 0.001, ** p < 0.01 vs. control.

    Article Snippet: Human pulmonary artery microvascular endothelial cells (HMEC, American Type Culture Collection (ATCC)) were cultured in endothelial cell media as described above, in an incubator at 37°C with 5% CO 2 .

    Techniques: Recombinant, Marker, Isolation, Control

    Effects of endotoxin‐depleted N‐protein (NPd) on inflammatory marker mRNA levels in mouse microvascular endothelial cells. Cells were treated with vehicle, LPS (1 μg/mL), NPd (50 μg/mL), NPd + PMB (250 μg/mL), or PMB (250 μg/mL) alone (control) for 4 h. Total RNA was isolated and RT‐qPCR analysis was performed to measure mRNA levels of TNFα and E‐selectin. Data are presented as mean ± SEM and analyzed using one‐way ANOVA followed by Bonferroni's multiple comparisons test. n = 3 independent cell cultures per group. *** p < 0.001, ** p < 0.01 vs. control.

    Journal: The FASEB Journal

    Article Title: SARS ‐ CoV ‐2 Nucleocapsid Protein Does Not Induce Inflammation in Endothelial Cells or Monocytes

    doi: 10.1096/fj.202501433R

    Figure Lengend Snippet: Effects of endotoxin‐depleted N‐protein (NPd) on inflammatory marker mRNA levels in mouse microvascular endothelial cells. Cells were treated with vehicle, LPS (1 μg/mL), NPd (50 μg/mL), NPd + PMB (250 μg/mL), or PMB (250 μg/mL) alone (control) for 4 h. Total RNA was isolated and RT‐qPCR analysis was performed to measure mRNA levels of TNFα and E‐selectin. Data are presented as mean ± SEM and analyzed using one‐way ANOVA followed by Bonferroni's multiple comparisons test. n = 3 independent cell cultures per group. *** p < 0.001, ** p < 0.01 vs. control.

    Article Snippet: Human pulmonary artery microvascular endothelial cells (HMEC, American Type Culture Collection (ATCC)) were cultured in endothelial cell media as described above, in an incubator at 37°C with 5% CO 2 .

    Techniques: Marker, Control, Isolation, Quantitative RT-PCR