pcrispr plasmids (Addgene inc)
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Addgene inc
pcrispr plasmids
Pcrispr Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcrispr+plasmid/pCRISPR+(Plasmid+%23113252)/pm41690159-96-19-24
Average 93 stars, based on 6 article reviews
Pcrispr Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcrispr+plasmid/pCRISPR+(Plasmid+%23113252)/pm41690159-96-19-24
Average 93 stars, based on 6 article reviews
pcrispr plasmids - by Bioz Stars,
2026-10
93/100 stars
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Related Articles
Plasmid Preparation:Article Title: Tau mediated coupling between Pol III synthesis and DnaB helicase unwinding helps maintain genomic stability. Article Snippet: The parental E. coli strain, MG1655, was used to create genetically edited CRISPR/Cas9 mutants of dnaX gene using a dual vector targeting system. .. Synthetic 40 base pair oligonucleotides with sequences homologous to dnaX targeting region acting as gRNA (Table S3) were ligated into the Article Title: Replisomal coupling between the α-Pol III core and the τ-subunit of the clamp loader complex (CLC) are essential for genomic integrity in E. coli. Article Snippet: Bacterial CRISPR-Cas9 genome editing The parental E. coli strain, MG1655, was used to create genetically edited CRISPR/Cas9 mutants of the dnaE gene using a dual vector targeting system (strains listed in Table S3). .. Synthetic ~40 base oligonucleotides with sequences homologous to the dnaE target region acting as guide RNA (gRNA) (Table S2) were ligated into the Article Title: Targeted chromosomal Escherichia coli:dnaB exterior surface residues regulate DNA helicase behavior to maintain genomic stability and organismal fitness. Article Snippet: .. Briefly, 30 base regions of the dnaB gene (S2 Table) targeting the mutation site were inserted into the Article Title: Tau-mediated coupling between Pol III synthesis and DnaB helicase unwinding helps maintain genomic stability Article Snippet: The parental E. coli strain, MG1655, was used to create genetically edited CRISPR/Cas9 mutants of dnaX gene using a dual vector targeting system. .. Synthetic 40 base pair oligonucleotides with sequences homologous to dnaX targeting region acting as gRNA ( ) were ligated into the Article Title: Targeted chromosomal Escherichia coli : dnaB exterior surface residues regulate DNA helicase behavior to maintain genomic stability and organismal fitness Article Snippet: .. Briefly, 30 base regions of the dnaB gene ( ) targeting the mutation site were inserted into the Article Title: An intramolecular cross-talk in D29 mycobacteriophage endolysin governs the lytic cycle and phage-host population dynamics Article Snippet: .. This selection strain harbored a Mutagenesis:Article Title: Targeted chromosomal Escherichia coli:dnaB exterior surface residues regulate DNA helicase behavior to maintain genomic stability and organismal fitness. Article Snippet: .. Briefly, 30 base regions of the dnaB gene (S2 Table) targeting the mutation site were inserted into the Article Title: Targeted chromosomal Escherichia coli : dnaB exterior surface residues regulate DNA helicase behavior to maintain genomic stability and organismal fitness Article Snippet: .. Briefly, 30 base regions of the dnaB gene ( ) targeting the mutation site were inserted into the other:Article Title: Replisomal coupling between the α-pol III core and the τ-subunit of the clamp loader complex (CLC) are essential for genomic integrity in Escherichia coli Article Snippet: Subsequently, 8 mM ATP, 2.4 ml of T4 DNA ligase (New England Biolabs), and 10 mM DTT were added and incubated at 16 °C for 18 h. The PEG and ethanol precipitation steps were repeated before resuspending the pellet in 100 μl of 1X Tris-EDTA buffer and storing at −20 °C. Selection:Article Title: An intramolecular cross-talk in D29 mycobacteriophage endolysin governs the lytic cycle and phage-host population dynamics Article Snippet: .. This selection strain harbored a |
