basic pcat vector (Promega)
90
Structured Review
Promega
basic pcat vector
Basic Pcat Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic/pcat+basic/pmc11193803-334-39-41
Average 90 stars, based on 1 article reviews
Basic Pcat Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic/pcat+basic/pmc11193803-334-39-41
Average 90 stars, based on 1 article reviews
basic pcat vector - by Bioz Stars,
2026-09
90/100 stars
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Synthesized:Article Title: Upstream Stimulatory Factor 2 Activates the Mammalian F 1 F 0 ATP Synthase α-Subunit Gene Through an Initiator Element Article Snippet: .. ATPA Inr Mutants Oligonucleotides containing base pair substitutions in the +68 to +86 bp region of the ATPA gene ( 25 ) were synthesized, annealed together, and then ligated into the reporter vector, Plasmid Preparation:Article Title: Upstream Stimulatory Factor 2 Activates the Mammalian F 1 F 0 ATP Synthase α-Subunit Gene Through an Initiator Element Article Snippet: .. ATPA Inr Mutants Oligonucleotides containing base pair substitutions in the +68 to +86 bp region of the ATPA gene ( 25 ) were synthesized, annealed together, and then ligated into the reporter vector, Article Title: Transcriptional regulation of the nuclear gene encoding the alpha-subunit of the mammalian mitochondrial F1F0 ATP synthase complex: role for the orphan nuclear receptor, COUP-TFII/ARP-1. Article Snippet: Our laboratory has been studying the transcriptional regulation of the nuclear gene (ATPA) that encodes the R-subunit of the mammalian mitochondrial F1F0 ATP synthase complex.. We have previously determined that the regulatory factor, upstream stimulatory factor 2 (USF2), can stimulate transcription of the ATPA gene through the cis-acting regulatory element 1 in the upstream promoter of this gene.. In this study, we used the yeast one-hybrid screening method to identify another factor, COUPTFII/ARP-1, which also binds to the ATPA cis-acting regulatory element 1. Article Title: Yellow fever mosquito sterol carrier protein-2 gene structure and transcriptional regulation Article Snippet: .. Plasmid construction and deletion analysis The 4.2-kb 5’ upstream flanking region ( ) was ligated to the bacterial chloramphenicol acetyltransferase gene (CAT) that had been excised from Construct:Article Title: The effects of lipopolysaccharide and interleukins-1alpha, -2 and -6 on oxytocin receptor expression and prostaglandin production in bovine endometrium. Article Snippet: .. Reporter constructs were prepared with a bovine OTR gene PstI fragment containing 1169 bp upstream from the transcription start site cloned into Article Title: A novel functional co-operation between MyoD, MEF2 and TRalpha1 is sufficient for the induction of GLUT4 gene transcription. Article Snippet: 0022-2836/01/020195±10 $35.00/0 We report tripartite co-operation between MyoD, myocyte enhancer factor-2 (MEF2) and the thyroid hormone receptor (TRa1) that takes place in the context of an 82-bp muscle-speci®c enhancer in the rat insulin-responsive glucose transporter (GLUT4) gene that is active in both cardiac and skeletal muscle.. In the L6E9 skeletal muscle cell line and in 10T1/2 ®broblasts, a powerful synergistic activation of the GLUT4 enhancer relied on the over-expression of MyoD, MEF2 and TRa1 and the integrity of their respective binding sites, and occurred when linked to either a heterologous promoter or in the context of the native GLUT4 promoter.. In cardiac myocytes, enhancer activity was dependent on the binding sites for MEF2 and TRa1. Article Title: Transcriptional regulation of the nuclear gene encoding the alpha-subunit of the mammalian mitochondrial F1F0 ATP synthase complex: role for the orphan nuclear receptor, COUP-TFII/ARP-1. Article Snippet: Our laboratory has been studying the transcriptional regulation of the nuclear gene (ATPA) that encodes the R-subunit of the mammalian mitochondrial F1F0 ATP synthase complex.. We have previously determined that the regulatory factor, upstream stimulatory factor 2 (USF2), can stimulate transcription of the ATPA gene through the cis-acting regulatory element 1 in the upstream promoter of this gene.. In this study, we used the yeast one-hybrid screening method to identify another factor, COUPTFII/ARP-1, which also binds to the ATPA cis-acting regulatory element 1. Article Title: The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs Article Snippet: .. All intron constructs carry the HcTnC4500 full promoter cloned into Clone Assay:Article Title: The effects of lipopolysaccharide and interleukins-1alpha, -2 and -6 on oxytocin receptor expression and prostaglandin production in bovine endometrium. Article Snippet: .. Reporter constructs were prepared with a bovine OTR gene PstI fragment containing 1169 bp upstream from the transcription start site cloned into Article Title: cAMP promotes pancreatic ?-cell survival via CREB-mediated induction of IRS2 Article Snippet: .. Generation of RIP A-CREB transgenic mice The 5′-flanking rat insulin1 promoter from –410 bp to +10 bp was cloned into the multicloning site of Article Title: The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs Article Snippet: .. All intron constructs carry the HcTnC4500 full promoter cloned into Sequencing:Article Title: The effects of lipopolysaccharide and interleukins-1alpha, -2 and -6 on oxytocin receptor expression and prostaglandin production in bovine endometrium. Article Snippet: .. Reporter constructs were prepared with a bovine OTR gene PstI fragment containing 1169 bp upstream from the transcription start site cloned into Amplification:Article Title: The effects of lipopolysaccharide and interleukins-1alpha, -2 and -6 on oxytocin receptor expression and prostaglandin production in bovine endometrium. Article Snippet: .. Reporter constructs were prepared with a bovine OTR gene PstI fragment containing 1169 bp upstream from the transcription start site cloned into Polymerase Chain Reaction:Article Title: The effects of lipopolysaccharide and interleukins-1alpha, -2 and -6 on oxytocin receptor expression and prostaglandin production in bovine endometrium. Article Snippet: .. Reporter constructs were prepared with a bovine OTR gene PstI fragment containing 1169 bp upstream from the transcription start site cloned into Cloning:Article Title: A novel functional co-operation between MyoD, MEF2 and TRalpha1 is sufficient for the induction of GLUT4 gene transcription. Article Snippet: 0022-2836/01/020195±10 $35.00/0 We report tripartite co-operation between MyoD, myocyte enhancer factor-2 (MEF2) and the thyroid hormone receptor (TRa1) that takes place in the context of an 82-bp muscle-speci®c enhancer in the rat insulin-responsive glucose transporter (GLUT4) gene that is active in both cardiac and skeletal muscle.. In the L6E9 skeletal muscle cell line and in 10T1/2 ®broblasts, a powerful synergistic activation of the GLUT4 enhancer relied on the over-expression of MyoD, MEF2 and TRa1 and the integrity of their respective binding sites, and occurred when linked to either a heterologous promoter or in the context of the native GLUT4 promoter.. In cardiac myocytes, enhancer activity was dependent on the binding sites for MEF2 and TRa1. other:Article Title: The coordination of the sequential appearance of MHR4 and dopa decarboxylase during the decline of the ecdysteroid titer at the end of the molt. Article Snippet: During the last larval molt in Manduca sexta, in response to an increasing, then decreasing ecdysteroid titer, a number of transcription factors uch as E75B, MHR3, MHR4, and FTZ-F1 appear and disappear in the abdominal epidermis leading to dopa decarboxylase (DDC) expression. essenger RNAs for both the 20E-induced transcription factors, MHR3 and E75B, are maximal near the peak of the ecdysteroid titer with MHR4 RNA appearing as the titer declines followed by FTZ-F1 and DDC mRNAs.. E75B and MHR4 mRNA were not expressed in Manduca GV1 ells, either during exposure to 20E or after its removal.. When either MHR3 dsRNA was transfected or E75B was constitutively expressed in these ells, MHR4 mRNA appeared in response to 20E by 6 h. E75B was found to form a heterodimer with MHR3 using the BacterioMatch II two-hybrid ssay. Transgenic Assay:Article Title: cAMP promotes pancreatic ?-cell survival via CREB-mediated induction of IRS2 Article Snippet: .. Generation of RIP A-CREB transgenic mice The 5′-flanking rat insulin1 promoter from –410 bp to +10 bp was cloned into the multicloning site of |
