anna katerina hadjantonakis (Addgene inc)
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anna katerina hadjantonakis
Anna Katerina Hadjantonakis, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcag-gfp/pCAG%3AGPI-GFP+(Plasmid+%2332601)/bio_rxiv__64898__2026__03__22__713470-230-19-21
Average 93 stars, based on 20 article reviews
Anna Katerina Hadjantonakis, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcag-gfp/pCAG%3AGPI-GFP+(Plasmid+%2332601)/bio_rxiv__64898__2026__03__22__713470-230-19-21
Average 93 stars, based on 20 article reviews
anna katerina hadjantonakis - by Bioz Stars,
2026-10
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Knock-Out:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Mouse Assay:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Recombinant:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Software:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Plasmid Preparation:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Incubation:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Paraffin Wax:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Binding Assay:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Blocking Assay:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Dissection:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Microscopy:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Electroporation:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Staining:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Transfection:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Immunofluorescence:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Expressing:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Enzyme-linked Immunosorbent Assay:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Western Blot:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Concentration Assay:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). In Vitro:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Control:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Two Tailed Test:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Immunostaining:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Comparison:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). Mass Spectrometry:Article Title: In situ cell-type-specific cell-surface proteomic profiling in mice. Article Snippet: Nelson; Clark et al., 2020 N/A Mouse: MADM6-GT Contreras et al., 2021 N/A Mouse: MADM6-TG Contreras et al., 2021 N/A Oligonucleotides See Table S2 for a complete list of sequences Recombinant DNA See Table S2 for a complete list of gR and Article Title: Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos Article Snippet: pCAG - GFP , Matsuda and Cepko 4 , pCAG-GFP was a gift from Article Title: PCSK9 deficiency alters brain lipid composition without affecting brain development and function Article Snippet: We transfected the cells with the PCSK9 plasmid or an empty pCAGEN vector and stained with an anti-PCSK9 antibody (Goat-a-PCSK9: AF3985 (anti mouse/rat PCSK9, 0.2 mg/mL), dilution 1:50) and used immunofluorescence to confirm specific PCSK9 expression. Article Title: DCX knockout ferret reveals a neurogenic mechanism in cortical development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Ferrets: WT This paper N/A Ferrets: DCX knockout mutants This paper N/A Mice: ICR This Article Title: Sensor systems for target ligands and uses thereof Article Snippet: Article Title: Polysaccharide-Polyplex Nanofilm Coatings Enhance Nanoneedle-Based Gene Delivery and Transfection Efficiency. Article Snippet: Anti-mouse CD44 was purchased from BD Biosciences. pCAG-GFP and Article Title: The mTOR pathway genes mTOR, Rheb, Depdc5, Pten , and Tsc1 have convergent and divergent impacts on cortical neuron development and function Article Snippet: pCAG-GFP , Article Title: Caveolin-3 prevents swelling-induced membrane damage via regulation of I Cl,swell activity. Article Snippet: Caveola membrane structures harbor mechanosensitive chloride channels (MCCs; including chloride channel 2, chloride channel 3, and SWELL1, also known as LRRC8A) that form a swelling-activated chloride current (ICl,swell) and play an important role in cell volume regulation and mechanoelectrical signal transduction.. However, the role of the muscle-specific caveolar scaffolding protein caveolin-3 (Cav3) in regulation of MCC expression, activity, and contribution to membrane integrity in response to mechanical stress remains unclear.. Here we showed that Cav3-transfected (Cav3-positive) HEK293 cells were significantly resistant to extreme (<20 milliosmole) hypotonic swelling compared with native (Cav3-negative) HEK293 cells; the percentage of cells with membrane damage decreased from 45% in Cav3-negative cells to 17% in Cav3-positive cells (p < 0.05). |
