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pcag gfp vector  (Addgene inc)


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    Structured Review

    Addgene inc pcag gfp vector
    Pcag Gfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcag+vector/pCAG-GFP+(Plasmid+%2311150)/pmc12637253-52-22-24
    Average 95 stars, based on 321 article reviews
    pcag gfp vector - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Expressing:

    Article Title: Engineering paralog-specific PSD-95 recombinant binders as minimally interfering multimodal probes for advanced imaging techniques
    Article Snippet: .. Plasmids for soluble Xph clone 656 expression were obtained by replacing the eGFP-CCR5 ZF-KRAB(A) fragment from the corresponding pCAG vector (gift from Don 657 Arnold, USC, Addgene #46295) (Gross et al., 2013) by an octa-His and HA tags using BglII and BsrGI restriction sites. .. Plasmid for 658 soluble PSD-95 PDZ domain 2 expression was obtained by first replacing eGFP into pEGFP-N1 by mIRFP via BamHI and BsrGI 659 restriction sites (gift from M Davidson, Florida State University, and X Shu, UCSF, Addgene #54620 )(Yu et al., 2015) and then 660 subcloning the PDZ domain using HindIII and BamHI restriction sites.

    Article Title: Engineering paralog-specific PSD-95 recombinant binders as minimally interfering multimodal probes for advanced imaging techniques
    Article Snippet: .. Plasmids for soluble Xph clone expression were obtained by replacing the eGFP-CCR5 ZF-KRAB(A) fragment from the corresponding pCAG vector (gift from Don Arnold, USC, Addgene #46295; ) by an octa-His and HA tags using BglII and BsrGI restriction sites. .. Plasmid for soluble PSD-95 PDZ domain 2 expression was obtained by first replacing eGFP into pEGFP-N1 by mIRFP via BamHI and BsrGI restriction sites (gift from M Davidson, Florida State University, and X Shu, UCSF, Addgene #54620; ) and then subcloning the PDZ domain using HindIII and BamHI restriction sites.

    Plasmid Preparation:

    Article Title: Engineering paralog-specific PSD-95 recombinant binders as minimally interfering multimodal probes for advanced imaging techniques
    Article Snippet: .. Plasmids for soluble Xph clone 656 expression were obtained by replacing the eGFP-CCR5 ZF-KRAB(A) fragment from the corresponding pCAG vector (gift from Don 657 Arnold, USC, Addgene #46295) (Gross et al., 2013) by an octa-His and HA tags using BglII and BsrGI restriction sites. .. Plasmid for 658 soluble PSD-95 PDZ domain 2 expression was obtained by first replacing eGFP into pEGFP-N1 by mIRFP via BamHI and BsrGI 659 restriction sites (gift from M Davidson, Florida State University, and X Shu, UCSF, Addgene #54620 )(Yu et al., 2015) and then 660 subcloning the PDZ domain using HindIII and BamHI restriction sites.

    Article Title: Inceptor binds to and directs insulin towards lysosomal degradation in β cells
    Article Snippet: .. The resulting HaloTag constructs were subcloned into a pCAG vector (Addgene vector no. 79009 modified with an SV40 ori /pA) by EcoRI and NheI digestion. ..

    Article Title: A step towards animal models with self-sustained fungal bioluminescence
    Article Snippet: For overexpression experiments in HEK 293T cells, both nnLuz_v3 and iRFP670 DNA sequences were synthesized as dsDNA Gene Fragments (Twist Bioscience). .. DNA fragments were amplified by PCR and cloned into the pCAG vector (pCAG-GFP, Addgene plasmid 11150) through Gibson assembly method using the NEBuilder HiFi DNA Assembly Cloning Kit (E5520S, NEB) according to manufacturer instructions. ..

    Article Title: Rapid fluorescence lifetime sensor development of LifeCamp enables transient and baseline absolute calcium measurements
    Article Snippet: After codon optimization, constructs were produced by Twist Biosciences and cloned into a commercially available pAAV-hSyn containing plasmid (#172921, Twist Biosciences). .. To express constructs in HEK293T cells, the clonal gene was subcloned into a pCAG vector (Addgene, #11150), using PCR followed by Gibson assembly of each construct. .. Soma-targeted LifeCamp was generated by adding a RiboL1 sequence, as has been previously utilized (Addgene, #349877), but not characterized further here.

    Article Title: Roles of class III phosphatidylinositol 3-kinase, Vps34, in phagocytosis, autophagy, and endocytosis in retinal pigmented epithelium
    Article Snippet: Mouse WD repeat domain phosphoinositide-interacting protein 2 (WIPI2) cDNA was cloned from mouse retina phage cDNA (ʎZAPII) library (a gift from Wolfgang Baehr, University of Utah) using primers 5′-ATGAACCTGGCGAGCCAGAGCGG AGAGGCC-3′ and 5′-ACAGCGAACATCCTCCCATGATT CTCCGGACTGAC-3′, and the sequence was confirmed as identical to NM_178398.4 . , , NM_178398.4. .. Two tandem copies of Hrs, FAPP1, or TAPP1, three tandem copies of ING2, or one copy of PLCδ phosphoinositide-binding domains, or WIPI1 or WIPI2 cDNA were cloned with N-terminal EGFP or DsRed fusions in pCAG vector (a gift from Connie Cepko (Addgene plasmid #11150)) Matsuda & Cepko, using EcoRI and NotI sites. All constructs were verified by sequencing. , Addgene , #11150. ..

    Article Title: Roles of class III phosphatidylinositol 3-kinase, Vps34, in phagocytosis, autophagy, and endocytosis in retinal pigmented epithelium
    Article Snippet: .. Two tandem copies of Hrs, FAPP1, or TAPP1, three tandem copies of ING2, or one copy of PLCδ phosphoinositide-binding domains, or WIPI1 or WIPI2 cDNA were cloned with N-terminal EGFP or DsRed fusions in pCAG vector (a gift from Connie Cepko, Addgene plasmid #11150) using EcoRI and NotI sites. ..

    Article Title: Inceptor binds to and directs insulin towards lysosomal degradation in β cells.
    Article Snippet: .. The resulting HaloTag constructs were subcloned into a pCAG vector (Addgene vector no. 79009 modified with an SV40 ori /pA) by EcoRI and NheI digestion. ..

    Construct:

    Article Title: Inceptor binds to and directs insulin towards lysosomal degradation in β cells
    Article Snippet: .. The resulting HaloTag constructs were subcloned into a pCAG vector (Addgene vector no. 79009 modified with an SV40 ori /pA) by EcoRI and NheI digestion. ..

    Article Title: Rapid fluorescence lifetime sensor development of LifeCamp enables transient and baseline absolute calcium measurements
    Article Snippet: After codon optimization, constructs were produced by Twist Biosciences and cloned into a commercially available pAAV-hSyn containing plasmid (#172921, Twist Biosciences). .. To express constructs in HEK293T cells, the clonal gene was subcloned into a pCAG vector (Addgene, #11150), using PCR followed by Gibson assembly of each construct. .. Soma-targeted LifeCamp was generated by adding a RiboL1 sequence, as has been previously utilized (Addgene, #349877), but not characterized further here.

    Article Title: Roles of class III phosphatidylinositol 3-kinase, Vps34, in phagocytosis, autophagy, and endocytosis in retinal pigmented epithelium
    Article Snippet: Mouse WD repeat domain phosphoinositide-interacting protein 2 (WIPI2) cDNA was cloned from mouse retina phage cDNA (ʎZAPII) library (a gift from Wolfgang Baehr, University of Utah) using primers 5′-ATGAACCTGGCGAGCCAGAGCGG AGAGGCC-3′ and 5′-ACAGCGAACATCCTCCCATGATT CTCCGGACTGAC-3′, and the sequence was confirmed as identical to NM_178398.4 . , , NM_178398.4. .. Two tandem copies of Hrs, FAPP1, or TAPP1, three tandem copies of ING2, or one copy of PLCδ phosphoinositide-binding domains, or WIPI1 or WIPI2 cDNA were cloned with N-terminal EGFP or DsRed fusions in pCAG vector (a gift from Connie Cepko (Addgene plasmid #11150)) Matsuda & Cepko, using EcoRI and NotI sites. All constructs were verified by sequencing. , Addgene , #11150. ..

    Article Title: Inceptor binds to and directs insulin towards lysosomal degradation in β cells.
    Article Snippet: .. The resulting HaloTag constructs were subcloned into a pCAG vector (Addgene vector no. 79009 modified with an SV40 ori /pA) by EcoRI and NheI digestion. ..

    Modification:

    Article Title: Inceptor binds to and directs insulin towards lysosomal degradation in β cells
    Article Snippet: .. The resulting HaloTag constructs were subcloned into a pCAG vector (Addgene vector no. 79009 modified with an SV40 ori /pA) by EcoRI and NheI digestion. ..

    Article Title: Inceptor binds to and directs insulin towards lysosomal degradation in β cells.
    Article Snippet: .. The resulting HaloTag constructs were subcloned into a pCAG vector (Addgene vector no. 79009 modified with an SV40 ori /pA) by EcoRI and NheI digestion. ..

    Amplification:

    Article Title: A step towards animal models with self-sustained fungal bioluminescence
    Article Snippet: For overexpression experiments in HEK 293T cells, both nnLuz_v3 and iRFP670 DNA sequences were synthesized as dsDNA Gene Fragments (Twist Bioscience). .. DNA fragments were amplified by PCR and cloned into the pCAG vector (pCAG-GFP, Addgene plasmid 11150) through Gibson assembly method using the NEBuilder HiFi DNA Assembly Cloning Kit (E5520S, NEB) according to manufacturer instructions. ..

    Polymerase Chain Reaction:

    Article Title: A step towards animal models with self-sustained fungal bioluminescence
    Article Snippet: For overexpression experiments in HEK 293T cells, both nnLuz_v3 and iRFP670 DNA sequences were synthesized as dsDNA Gene Fragments (Twist Bioscience). .. DNA fragments were amplified by PCR and cloned into the pCAG vector (pCAG-GFP, Addgene plasmid 11150) through Gibson assembly method using the NEBuilder HiFi DNA Assembly Cloning Kit (E5520S, NEB) according to manufacturer instructions. ..

    Article Title: Rapid fluorescence lifetime sensor development of LifeCamp enables transient and baseline absolute calcium measurements
    Article Snippet: After codon optimization, constructs were produced by Twist Biosciences and cloned into a commercially available pAAV-hSyn containing plasmid (#172921, Twist Biosciences). .. To express constructs in HEK293T cells, the clonal gene was subcloned into a pCAG vector (Addgene, #11150), using PCR followed by Gibson assembly of each construct. .. Soma-targeted LifeCamp was generated by adding a RiboL1 sequence, as has been previously utilized (Addgene, #349877), but not characterized further here.

    Clone Assay:

    Article Title: A step towards animal models with self-sustained fungal bioluminescence
    Article Snippet: For overexpression experiments in HEK 293T cells, both nnLuz_v3 and iRFP670 DNA sequences were synthesized as dsDNA Gene Fragments (Twist Bioscience). .. DNA fragments were amplified by PCR and cloned into the pCAG vector (pCAG-GFP, Addgene plasmid 11150) through Gibson assembly method using the NEBuilder HiFi DNA Assembly Cloning Kit (E5520S, NEB) according to manufacturer instructions. ..

    Article Title: Roles of class III phosphatidylinositol 3-kinase, Vps34, in phagocytosis, autophagy, and endocytosis in retinal pigmented epithelium
    Article Snippet: Mouse WD repeat domain phosphoinositide-interacting protein 2 (WIPI2) cDNA was cloned from mouse retina phage cDNA (ʎZAPII) library (a gift from Wolfgang Baehr, University of Utah) using primers 5′-ATGAACCTGGCGAGCCAGAGCGG AGAGGCC-3′ and 5′-ACAGCGAACATCCTCCCATGATT CTCCGGACTGAC-3′, and the sequence was confirmed as identical to NM_178398.4 . , , NM_178398.4. .. Two tandem copies of Hrs, FAPP1, or TAPP1, three tandem copies of ING2, or one copy of PLCδ phosphoinositide-binding domains, or WIPI1 or WIPI2 cDNA were cloned with N-terminal EGFP or DsRed fusions in pCAG vector (a gift from Connie Cepko (Addgene plasmid #11150)) Matsuda & Cepko, using EcoRI and NotI sites. All constructs were verified by sequencing. , Addgene , #11150. ..

    Article Title: Roles of class III phosphatidylinositol 3-kinase, Vps34, in phagocytosis, autophagy, and endocytosis in retinal pigmented epithelium
    Article Snippet: .. Two tandem copies of Hrs, FAPP1, or TAPP1, three tandem copies of ING2, or one copy of PLCδ phosphoinositide-binding domains, or WIPI1 or WIPI2 cDNA were cloned with N-terminal EGFP or DsRed fusions in pCAG vector (a gift from Connie Cepko, Addgene plasmid #11150) using EcoRI and NotI sites. ..

    Cloning:

    Article Title: A step towards animal models with self-sustained fungal bioluminescence
    Article Snippet: For overexpression experiments in HEK 293T cells, both nnLuz_v3 and iRFP670 DNA sequences were synthesized as dsDNA Gene Fragments (Twist Bioscience). .. DNA fragments were amplified by PCR and cloned into the pCAG vector (pCAG-GFP, Addgene plasmid 11150) through Gibson assembly method using the NEBuilder HiFi DNA Assembly Cloning Kit (E5520S, NEB) according to manufacturer instructions. ..

    Sequencing:

    Article Title: Roles of class III phosphatidylinositol 3-kinase, Vps34, in phagocytosis, autophagy, and endocytosis in retinal pigmented epithelium
    Article Snippet: Mouse WD repeat domain phosphoinositide-interacting protein 2 (WIPI2) cDNA was cloned from mouse retina phage cDNA (ʎZAPII) library (a gift from Wolfgang Baehr, University of Utah) using primers 5′-ATGAACCTGGCGAGCCAGAGCGG AGAGGCC-3′ and 5′-ACAGCGAACATCCTCCCATGATT CTCCGGACTGAC-3′, and the sequence was confirmed as identical to NM_178398.4 . , , NM_178398.4. .. Two tandem copies of Hrs, FAPP1, or TAPP1, three tandem copies of ING2, or one copy of PLCδ phosphoinositide-binding domains, or WIPI1 or WIPI2 cDNA were cloned with N-terminal EGFP or DsRed fusions in pCAG vector (a gift from Connie Cepko (Addgene plasmid #11150)) Matsuda & Cepko, using EcoRI and NotI sites. All constructs were verified by sequencing. , Addgene , #11150. ..



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    Excitatory and inhibitory cerebellar projections at P0 . (A) 3D visualization of excitatory (eCb; Ntsr1 Cre +AAV-FLEX-EGFP ) and (B) inhibitory (iCb; Vgat1 Cre +AAV-FLEX-EGFP ) axonal bundles at P0 in the whole brain.
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    Image Search Results


    Excitatory and inhibitory cerebellar projections at P0 . (A) 3D visualization of excitatory (eCb; Ntsr1 Cre +AAV-FLEX-EGFP ) and (B) inhibitory (iCb; Vgat1 Cre +AAV-FLEX-EGFP ) axonal bundles at P0 in the whole brain.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Brain-wide mapping of developmental trajectories of cerebellar efferent projections

    doi: 10.1073/pnas.2521091122

    Figure Lengend Snippet: Excitatory and inhibitory cerebellar projections at P0 . (A) 3D visualization of excitatory (eCb; Ntsr1 Cre +AAV-FLEX-EGFP ) and (B) inhibitory (iCb; Vgat1 Cre +AAV-FLEX-EGFP ) axonal bundles at P0 in the whole brain.

    Article Snippet: To anterogradely trace cerebellar long-range projections, we utilized an EGFP lentivirus (LV-H1p-UbCpEGFP) in wild-type mice and Cre-dependent viral vectors pAAV CAG-FLEX-EGFP-WPRE (Addgene #51502, AAV1) or pAAV hSyn.FLEX.mGFP.2A.Synaptophysin-mRuby (Addgene #71760, AAV1) in Ntsr1 Cre and Vgat1 Cre mice.

    Techniques:

    Development of eCb–hindbrain projections . 3D visualization of the development of excitatory cerebellar (eCb; Ntsr1 Cre +AAV-FLEX-EGFP ) efferent projections innervating the hindbrain. The movie highlights the progressive expansion of eCb axonal pathways across hindbrain regions.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Brain-wide mapping of developmental trajectories of cerebellar efferent projections

    doi: 10.1073/pnas.2521091122

    Figure Lengend Snippet: Development of eCb–hindbrain projections . 3D visualization of the development of excitatory cerebellar (eCb; Ntsr1 Cre +AAV-FLEX-EGFP ) efferent projections innervating the hindbrain. The movie highlights the progressive expansion of eCb axonal pathways across hindbrain regions.

    Article Snippet: To anterogradely trace cerebellar long-range projections, we utilized an EGFP lentivirus (LV-H1p-UbCpEGFP) in wild-type mice and Cre-dependent viral vectors pAAV CAG-FLEX-EGFP-WPRE (Addgene #51502, AAV1) or pAAV hSyn.FLEX.mGFP.2A.Synaptophysin-mRuby (Addgene #71760, AAV1) in Ntsr1 Cre and Vgat1 Cre mice.

    Techniques: