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liver pieces  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology liver pieces
    Liver Pieces, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 4521 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+buffer/Phosphate+Buffered+Saline/pm42888028-315-17-34
    Average 97 stars, based on 4521 article reviews
    liver pieces - by Bioz Stars, 2026-10
    97/100 stars

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    Incubation:

    Article Title: Gut microbiota composition combined with reduced intestinal fatty acid uptake prevents hepatic steatosis in obesity-resistant mice fed a high-fat diet
    Article Snippet: Protein isolates were boiled in Laemmli buffer for 5 min and 40 μg of proteins from whole cell extracts, cytoplasmic and mitochondrial fraction and 60 μg of proteins from nuclear fraction were resolved on 8% or 10% sodium dodecyl sulfate-polyacrylamide gels using the Mini-Protean II Electrophoresis Cell System (Bio-Rad Laboratories, Hercules, CA, USA). .. After protein transfer from the gels onto polyvinylidene fluoride membranes (Immobilon-FL, Merck Millipore, Billerica, MA, USA) in the Mini Trans-Blot Electrophoretic Transfer Cell System (Bio-Rad Laboratories, Hercules, CA, USA), the membranes were blocked with 2% BSA in PBS buffer, washed and incubated overnight at 4 °C with the primary antibodies: anti-CD36 (1:500, sc-7309, Santa Cruz Biotechnology), anti-FATP4 (1:500, sc-393309, Santa Cruz Biotechnology), anti-ZO-1 (1:1000, #40-2200, Thermo Fisher Scientific), anti-occludin (1:1000, ab216327, Abcam), anti-SREBP-1c (1:500, sc-366, Santa Cruz Biotechnology), anti-ChREBP (1:1000, NB400-135, Novus Biologicals), anti-FXR (1:500, sc-25309, Santa Cruz Biotechnology), anti-PPARα (1:500, sc-398394, Santa Cruz Biotechnology), anti-PGC-1α (1:500, ab54481, Abcam), anti-CPT1 (1:500, sc-139482, Santa Cruz Biotechnology), anti-perilipin-2 (1:1000, NB110-40877, Novus Biologicals), anti-GLUT2 (1:1000, ab192599, Abcam), anti-IRS1 (1:500, FNab04400, FineTest), anti-IRS2 (1:500, #3089, Cell Signaling), anti-phospho-AKT-Thr308 (1:500, sc-27196, Santa Cruz Biotechnology), anti-AKT (1:1000, #9272, Cell Signaling) and anti-NLRP3 (1:500, NBP2-12446, Novus Biologicals). .. The membranes were then washed and incubated with the corresponding horseradish peroxidase-conjugated secondary antibodies (Anti-Rabbit IgG: 1:20000, NB7160, Novus Biologicals; Anti-Mouse IgG: 1:30000, ab97046, Abcam).

    Article Title: Natural Variation of a Specific NLR Gene RGA4L Confers Strong Chilling Tolerance in Rice
    Article Snippet: .. For the pull‐down assay, 5 μg of purified GST/MBP‐fused RGA4L protein or GST/MBP and OsHSP90/OsLEA5‐MBP/GST proteins were incubated in 1 mL of PBS buffer (pH 7.4, 0.1% NP‐40) at 4°C with gentle agitation for 2 h before the addition of 20 μL of Protein A/G PLUS‐Agarose (Santa Cruz, sc‐2003) or Glutathione Sepharose 4B beads (Yeasen, 20507ES10). ..

    Article Title: Natural Variation of a Specific NLR Gene RGA4L Confers Strong Chilling Tolerance in Rice.
    Article Snippet: .. For the pull- down assay, 5 μg of purified GST/MBP- fused RGA4L protein or GST/MBP and OsHSP90/OsLEA5- MBP/GST proteins were incubated in 1 mL of PBS buffer (pH 7.4, 0.1% NP- 40) at 4°C with gentle agitation for 2 h before the addition of 20 μL of Protein A/G PLUS- Agarose (Santa Cruz, sc- 2003) or Glutathione Sepharose 4B beads (Yeasen, 20507ES10). ..

    Article Title: Impaired motor activity in a CRISPR SCA5 L253P knock-in mouse is associated with selective β-III-spectrin subcellular redistribution in the cerebellum
    Article Snippet: .. After blocking, the sections were incubated for 24 hours at 4°C in 2% donkey serum 0.3% Triton X-100 in PBS buffer with rabbit anti-Sptbn2 (1:1,000, Novus NB110-58346), mouse anti-Sptbn2 (1:1,000, Santa Cruz sc-515737) mouse ankyrin R clone N388A/10 (1-500, NeuroMAB 75-380), guinea pig anti-calbindin (1:1500, Synaptic Systems 214 004), F-Actin 488 (1:10,000 Phalloidin Abcam AB176753). ..

    Article Title: Oxytranscriptome of soybean seedlings under short-term cadmium treatment.
    Article Snippet: 10 mm behind the tip) were sectioned with the use of vibratome (Leica VT1200S, Germany) while submerged in a buffer bath (PBS, Bioshop, Canada, PBS404). .. The 100 μm thick sections were fixed in FAA (50% ethanol, 10% formalin, 5% glacial acetic acid), rinsed three times in PBS buffer and then incubated overnight in 4 °C with primary anti-8-OHG antibody (15A3, Santa Cruz Biotechnology, Inc., USA, sc-66036) diluted 1:50 with PBS/1% BSA. .. Subsequently, the sections were rinsed three times in PBS and then incubated at 37 °C for 2 h with FITC-conjugated anti-mouse secondary antibody (BP-FITC, Santa Cruz Biotechnology, Inc., USA, sc-516140) diluted 1:100 with PBS/1% BSA.

    Article Title: Selective Glycopolymer Inhibitors of Galectin-3: Supportive Anti-Cancer Agents Protecting Monocytes and Preserving Interferon-Gamma Function
    Article Snippet: .. The wells were then blocked by 2% w/v BSA in PBS buffer (250 μL/well) for 1 h. Serial dilutions of the ligands (glycopolymers or International Journal of Nanomedicine 2025:20 https://doi.org/10.2147/IJN.S503381 6599 Powered by TCPDF (www.tcpdf.org) oligosaccharides) in EPBS (2 mM EDTA in PBS) were incubated with Gal-1 or Gal-3 (1.25 μM final concentration) for 2 h. The residual bound galectin was labeled with anti-His antibody conjugated to horseradish peroxidase (Santa Cruz; 1:1000 dilution in PBS, 1 h). ..

    Article Title: Selective Glycopolymer Inhibitors of Galectin-3: Supportive Anti-Cancer Agents Protecting Monocytes and Preserving Interferon-Gamma Function
    Article Snippet: .. The wells were then blocked by 2% w/v BSA in PBS buffer (250 μL/well) for 1 h. Serial dilutions of the ligands (glycopolymers or oligosaccharides) in EPBS (2 mM EDTA in PBS) were incubated with Gal-1 or Gal-3 (1.25 μM final concentration) for 2 h. The residual bound galectin was labeled with anti-His antibody conjugated to horseradish peroxidase (Santa Cruz; 1:1000 dilution in PBS, 1 h). ..

    Article Title: Oxytranscriptome of soybean seedlings under short-term cadmium treatment
    Article Snippet: 10 mm behind the tip) were sectioned with the use of vibratome (Leica VT1200S, Germany) while submerged in a buffer bath (PBS, Bioshop, Canada, PBS404). .. The 100 μm thick sections were fixed in FAA (50% ethanol, 10% formalin, 5% glacial acetic acid), rinsed three times in PBS buffer and then incubated overnight in 4 °C with primary anti-8-OHG antibody (15A3, Santa Cruz Biotechnology, Inc., USA, sc-66036) diluted 1:50 with PBS/1% BSA. .. Subsequently, the sections were rinsed three times in PBS and then incubated at 37 °C for 2 h with FITC-conjugated anti-mouse secondary antibody (BP-FITC, Santa Cruz Biotechnology, Inc., USA, sc-516140) diluted 1:100 with PBS/1% BSA.

    Pull Down Assay:

    Article Title: Natural Variation of a Specific NLR Gene RGA4L Confers Strong Chilling Tolerance in Rice
    Article Snippet: .. For the pull‐down assay, 5 μg of purified GST/MBP‐fused RGA4L protein or GST/MBP and OsHSP90/OsLEA5‐MBP/GST proteins were incubated in 1 mL of PBS buffer (pH 7.4, 0.1% NP‐40) at 4°C with gentle agitation for 2 h before the addition of 20 μL of Protein A/G PLUS‐Agarose (Santa Cruz, sc‐2003) or Glutathione Sepharose 4B beads (Yeasen, 20507ES10). ..

    Article Title: Natural Variation of a Specific NLR Gene RGA4L Confers Strong Chilling Tolerance in Rice.
    Article Snippet: .. For the pull- down assay, 5 μg of purified GST/MBP- fused RGA4L protein or GST/MBP and OsHSP90/OsLEA5- MBP/GST proteins were incubated in 1 mL of PBS buffer (pH 7.4, 0.1% NP- 40) at 4°C with gentle agitation for 2 h before the addition of 20 μL of Protein A/G PLUS- Agarose (Santa Cruz, sc- 2003) or Glutathione Sepharose 4B beads (Yeasen, 20507ES10). ..

    Purification:

    Article Title: Natural Variation of a Specific NLR Gene RGA4L Confers Strong Chilling Tolerance in Rice
    Article Snippet: .. For the pull‐down assay, 5 μg of purified GST/MBP‐fused RGA4L protein or GST/MBP and OsHSP90/OsLEA5‐MBP/GST proteins were incubated in 1 mL of PBS buffer (pH 7.4, 0.1% NP‐40) at 4°C with gentle agitation for 2 h before the addition of 20 μL of Protein A/G PLUS‐Agarose (Santa Cruz, sc‐2003) or Glutathione Sepharose 4B beads (Yeasen, 20507ES10). ..

    Article Title: Natural Variation of a Specific NLR Gene RGA4L Confers Strong Chilling Tolerance in Rice.
    Article Snippet: .. For the pull- down assay, 5 μg of purified GST/MBP- fused RGA4L protein or GST/MBP and OsHSP90/OsLEA5- MBP/GST proteins were incubated in 1 mL of PBS buffer (pH 7.4, 0.1% NP- 40) at 4°C with gentle agitation for 2 h before the addition of 20 μL of Protein A/G PLUS- Agarose (Santa Cruz, sc- 2003) or Glutathione Sepharose 4B beads (Yeasen, 20507ES10). ..

    Gentle:

    Article Title: Natural Variation of a Specific NLR Gene RGA4L Confers Strong Chilling Tolerance in Rice
    Article Snippet: .. For the pull‐down assay, 5 μg of purified GST/MBP‐fused RGA4L protein or GST/MBP and OsHSP90/OsLEA5‐MBP/GST proteins were incubated in 1 mL of PBS buffer (pH 7.4, 0.1% NP‐40) at 4°C with gentle agitation for 2 h before the addition of 20 μL of Protein A/G PLUS‐Agarose (Santa Cruz, sc‐2003) or Glutathione Sepharose 4B beads (Yeasen, 20507ES10). ..

    Article Title: Natural Variation of a Specific NLR Gene RGA4L Confers Strong Chilling Tolerance in Rice.
    Article Snippet: .. For the pull- down assay, 5 μg of purified GST/MBP- fused RGA4L protein or GST/MBP and OsHSP90/OsLEA5- MBP/GST proteins were incubated in 1 mL of PBS buffer (pH 7.4, 0.1% NP- 40) at 4°C with gentle agitation for 2 h before the addition of 20 μL of Protein A/G PLUS- Agarose (Santa Cruz, sc- 2003) or Glutathione Sepharose 4B beads (Yeasen, 20507ES10). ..

    Blocking Assay:

    Article Title: Impaired motor activity in a CRISPR SCA5 L253P knock-in mouse is associated with selective β-III-spectrin subcellular redistribution in the cerebellum
    Article Snippet: .. After blocking, the sections were incubated for 24 hours at 4°C in 2% donkey serum 0.3% Triton X-100 in PBS buffer with rabbit anti-Sptbn2 (1:1,000, Novus NB110-58346), mouse anti-Sptbn2 (1:1,000, Santa Cruz sc-515737) mouse ankyrin R clone N388A/10 (1-500, NeuroMAB 75-380), guinea pig anti-calbindin (1:1500, Synaptic Systems 214 004), F-Actin 488 (1:10,000 Phalloidin Abcam AB176753). ..

    Concentration Assay:

    Article Title: Selective Glycopolymer Inhibitors of Galectin-3: Supportive Anti-Cancer Agents Protecting Monocytes and Preserving Interferon-Gamma Function
    Article Snippet: .. The wells were then blocked by 2% w/v BSA in PBS buffer (250 μL/well) for 1 h. Serial dilutions of the ligands (glycopolymers or International Journal of Nanomedicine 2025:20 https://doi.org/10.2147/IJN.S503381 6599 Powered by TCPDF (www.tcpdf.org) oligosaccharides) in EPBS (2 mM EDTA in PBS) were incubated with Gal-1 or Gal-3 (1.25 μM final concentration) for 2 h. The residual bound galectin was labeled with anti-His antibody conjugated to horseradish peroxidase (Santa Cruz; 1:1000 dilution in PBS, 1 h). ..

    Article Title: Selective Glycopolymer Inhibitors of Galectin-3: Supportive Anti-Cancer Agents Protecting Monocytes and Preserving Interferon-Gamma Function
    Article Snippet: .. The wells were then blocked by 2% w/v BSA in PBS buffer (250 μL/well) for 1 h. Serial dilutions of the ligands (glycopolymers or oligosaccharides) in EPBS (2 mM EDTA in PBS) were incubated with Gal-1 or Gal-3 (1.25 μM final concentration) for 2 h. The residual bound galectin was labeled with anti-His antibody conjugated to horseradish peroxidase (Santa Cruz; 1:1000 dilution in PBS, 1 h). ..

    Labeling:

    Article Title: Selective Glycopolymer Inhibitors of Galectin-3: Supportive Anti-Cancer Agents Protecting Monocytes and Preserving Interferon-Gamma Function
    Article Snippet: .. The wells were then blocked by 2% w/v BSA in PBS buffer (250 μL/well) for 1 h. Serial dilutions of the ligands (glycopolymers or International Journal of Nanomedicine 2025:20 https://doi.org/10.2147/IJN.S503381 6599 Powered by TCPDF (www.tcpdf.org) oligosaccharides) in EPBS (2 mM EDTA in PBS) were incubated with Gal-1 or Gal-3 (1.25 μM final concentration) for 2 h. The residual bound galectin was labeled with anti-His antibody conjugated to horseradish peroxidase (Santa Cruz; 1:1000 dilution in PBS, 1 h). ..

    Article Title: Selective Glycopolymer Inhibitors of Galectin-3: Supportive Anti-Cancer Agents Protecting Monocytes and Preserving Interferon-Gamma Function
    Article Snippet: .. The wells were then blocked by 2% w/v BSA in PBS buffer (250 μL/well) for 1 h. Serial dilutions of the ligands (glycopolymers or oligosaccharides) in EPBS (2 mM EDTA in PBS) were incubated with Gal-1 or Gal-3 (1.25 μM final concentration) for 2 h. The residual bound galectin was labeled with anti-His antibody conjugated to horseradish peroxidase (Santa Cruz; 1:1000 dilution in PBS, 1 h). ..



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    Methodological workflow from sample preparation to measuring the cellular response capacity (CRC). Following blood collection, samples are prepared for flow cytometric analysis either unstimulated (PBS as buffer control) or stimulated with an inflammatory cocktail (cocktail of N-formylmethionyl-leucyl-phenylalanine, platelet-activating factor, and tumor necrosis factor). The CRC is calculated as the ratio of median fluorescence intensity (MFI) between stimulated and unstimulated neutrophils. Three approaches, classic, simple, and kinetic CRC, offer distinct advantages and limitations based on technical aspects such as manual processing steps (e.g., centrifugation) and incubation time. n =13–14. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing patients with sepsis at all time points (shown in the figure: 0 h, not shown in the figure: 24, 72, 120 h) with healthy volunteers (HV). P -values are indicated above the respective data points. Asterisks indicate significant differences between HV and patients at 0 h only. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. AUC. Area under the curve; AU. Arbitrary units; PBS. Phosphate-buffered saline; E. coli Escherichia coli .

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Methodological workflow from sample preparation to measuring the cellular response capacity (CRC). Following blood collection, samples are prepared for flow cytometric analysis either unstimulated (PBS as buffer control) or stimulated with an inflammatory cocktail (cocktail of N-formylmethionyl-leucyl-phenylalanine, platelet-activating factor, and tumor necrosis factor). The CRC is calculated as the ratio of median fluorescence intensity (MFI) between stimulated and unstimulated neutrophils. Three approaches, classic, simple, and kinetic CRC, offer distinct advantages and limitations based on technical aspects such as manual processing steps (e.g., centrifugation) and incubation time. n =13–14. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing patients with sepsis at all time points (shown in the figure: 0 h, not shown in the figure: 24, 72, 120 h) with healthy volunteers (HV). P -values are indicated above the respective data points. Asterisks indicate significant differences between HV and patients at 0 h only. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. AUC. Area under the curve; AU. Arbitrary units; PBS. Phosphate-buffered saline; E. coli Escherichia coli .

    Article Snippet: Subsequently, the monovettes were exposed to either phosphate-buffered saline (PBS) with calcium and magnesium (PBS +/+ , #14080055, Gibco, Thermo Fisher Scientific, Waltham, USA) and the bacterial culture media [buffer control of Escherichia coli ( E. coli ) suspension, hereafter referred to as BuC], viable E. coli bacteria (ATCC line 25922, DSMZ, Braunschweig, Germany), or LPS (100 ng/ml, from E. coli O55:B5, #L2637, Sigma Aldrich, Steinheim, Germany).

    Techniques: Sample Prep, Control, Fluorescence, Centrifugation, Incubation, Saline

    Comparison of different approaches to measure the cellular response capacity (CRC) for CD11b on neutrophil granulocytes. Blood from healthy volunteers was incubated with either PBS (Control) or 100 ng/ml LPS for 60 min in the ex vivo whole blood model. a Analysis of the median fluorescence intensity (MFI). b Evaluation of the CRC as determined by different approaches (classic, simple, and kinetic CRC). c, d Change in fluorescence intensity and change in CRC using the kinetic CRC approach, comparing blood with previous exposure to LPS or PBS (buffer control) from the ex vivo whole blood model. In both c and d , the single-cell values measured by flow cytometry were condensed using a moving median with a window of 9 cells. This moving median was then approximated with a 5 th -degree polynomial function. The plots display these polynomial functions together with the baseline (median before stimulation) and a connecting line from the baseline to the polynomial function for 30 s after stimulation. Values are shown as median and interquartile range. n= 10. Statistical analysis was performed using the Mann-Whitney U test. ⁎⁎⁎ P <0.001. AU. Arbitrary units; LPS. Lipopolysaccharide; PBS. Phosphate-buffered saline.

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Comparison of different approaches to measure the cellular response capacity (CRC) for CD11b on neutrophil granulocytes. Blood from healthy volunteers was incubated with either PBS (Control) or 100 ng/ml LPS for 60 min in the ex vivo whole blood model. a Analysis of the median fluorescence intensity (MFI). b Evaluation of the CRC as determined by different approaches (classic, simple, and kinetic CRC). c, d Change in fluorescence intensity and change in CRC using the kinetic CRC approach, comparing blood with previous exposure to LPS or PBS (buffer control) from the ex vivo whole blood model. In both c and d , the single-cell values measured by flow cytometry were condensed using a moving median with a window of 9 cells. This moving median was then approximated with a 5 th -degree polynomial function. The plots display these polynomial functions together with the baseline (median before stimulation) and a connecting line from the baseline to the polynomial function for 30 s after stimulation. Values are shown as median and interquartile range. n= 10. Statistical analysis was performed using the Mann-Whitney U test. ⁎⁎⁎ P <0.001. AU. Arbitrary units; LPS. Lipopolysaccharide; PBS. Phosphate-buffered saline.

    Article Snippet: Subsequently, the monovettes were exposed to either phosphate-buffered saline (PBS) with calcium and magnesium (PBS +/+ , #14080055, Gibco, Thermo Fisher Scientific, Waltham, USA) and the bacterial culture media [buffer control of Escherichia coli ( E. coli ) suspension, hereafter referred to as BuC], viable E. coli bacteria (ATCC line 25922, DSMZ, Braunschweig, Germany), or LPS (100 ng/ml, from E. coli O55:B5, #L2637, Sigma Aldrich, Steinheim, Germany).

    Techniques: Comparison, Incubation, Control, Ex Vivo, Fluorescence, Single Cell, Flow Cytometry, MANN-WHITNEY, Saline

    Characterization of extracellular vesicles (EVs) derived from conditioned medium (CM). (a) Particle size distribution and concentration of EVs measured by nanoparticle tracking analysis. Median particle diameter: 143.6 nm; particle concentration: 1.6 × 10 9 particles/mL per 1 mL of unpurified CM prior to EV isolation. (b) Zeta potential of EVs measured in phosphate-buffered saline, showing a negatively charged surface of −36.38 mV. (c) Quantification of CD63-positive EVs using a Tim4–CD63 sandwich enzyme-linked immunosorbent assay. EV concentration is expressed per 1 mL of pre-purified CM. Statistical analysis using Welch's t -test demonstrated a significant difference between CM and EV samples ( n = 3 per group, ∗ p < 0.001).

    Journal: Regenerative Therapy

    Article Title: Dental pulp cell–derived conditioned culture media promotes periodontal repair through immunomodulation and bone regeneration

    doi: 10.1016/j.reth.2026.101144

    Figure Lengend Snippet: Characterization of extracellular vesicles (EVs) derived from conditioned medium (CM). (a) Particle size distribution and concentration of EVs measured by nanoparticle tracking analysis. Median particle diameter: 143.6 nm; particle concentration: 1.6 × 10 9 particles/mL per 1 mL of unpurified CM prior to EV isolation. (b) Zeta potential of EVs measured in phosphate-buffered saline, showing a negatively charged surface of −36.38 mV. (c) Quantification of CD63-positive EVs using a Tim4–CD63 sandwich enzyme-linked immunosorbent assay. EV concentration is expressed per 1 mL of pre-purified CM. Statistical analysis using Welch's t -test demonstrated a significant difference between CM and EV samples ( n = 3 per group, ∗ p < 0.001).

    Article Snippet: For subsequent passaging, the medium was removed and the dishes were rinsed twice with phosphate-buffered saline (Nacalai Tesque).

    Techniques: Derivative Assay, Concentration Assay, Isolation, Zeta Potential Analyzer, Saline, Sandwich ELISA, Purification