pbluescriptiiks (Thermo Fisher)
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pbluescriptiiks
Pbluescriptiiks, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescriptiiks/pbluescriptiiks/pmc07003650-117-344-346
Average 90 stars, based on 1 article reviews
Pbluescriptiiks, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescriptiiks/pbluescriptiiks/pmc07003650-117-344-346
Average 90 stars, based on 1 article reviews
pbluescriptiiks - by Bioz Stars,
2026-11
90/100 stars
Images
1) Product Images from "Gene ssfg_01967 ( miaB ) for tRNA modification influences morphogenesis and moenomycin biosynthesis in Streptomyces ghanaensis ATCC14672"
Article Title: Gene ssfg_01967 ( miaB ) for tRNA modification influences morphogenesis and moenomycin biosynthesis in Streptomyces ghanaensis ATCC14672
Journal: Microbiology
doi: 10.1099/mic.0.000747
Figure Legend Snippet: Strains and plasmids used in this work
Techniques Used: Plasmid Preparation, Expressing, Clone Assay, Disruption, Derivative Assay, Cloning, Selection, Marker
Related Articles
Amplification:Article Title: Transcriptional regulators of GntR family in Streptomyces coelicolor A3(2): analysis in silico and in vivo of YtrA subfamily. Article Snippet: Transcriptional factors of the GntR family regulate numerous physiological and morphological processes in response to the nutrient state of bacterial cells.. The number of GntR transcriptional factors in genomes of soil-dwelling actinomycetes is one of the highest among bacteria, reflecting both the large size of their chromosomes and the complex ecological niche that they occupy.. However, very little is known about the roles of GntRs in actinomycete biology. Clone Assay:Article Title: Transcriptional regulators of GntR family in Streptomyces coelicolor A3(2): analysis in silico and in vivo of YtrA subfamily. Article Snippet: Transcriptional factors of the GntR family regulate numerous physiological and morphological processes in response to the nutrient state of bacterial cells.. The number of GntR transcriptional factors in genomes of soil-dwelling actinomycetes is one of the highest among bacteria, reflecting both the large size of their chromosomes and the complex ecological niche that they occupy.. However, very little is known about the roles of GntRs in actinomycete biology. other:Article Title: Gene ssfg_01967 ( miaB ) for tRNA modification influences morphogenesis and moenomycin biosynthesis in Streptomyces ghanaensis ATCC14672 Article Snippet: Bacterial strain or plasmid Description* Source or reference E. coli DH5α supE44 ΔlacU169(φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 NEB E. coli ET12567 (pUZ8002) dam -13::Tn9(Cm r ) dcm -6 hsd M; harbors conjugative plasmid pUZ8002; Cm r , Km r C.P.Smith, UMIST, UK; E. coli WM6026 lacI q rrnB3 Δ lacZ 4787 hsdR514 Δ araBAD567 Δ rhaBAD568 rph-1 att λ::pAE12 (Δ ori R6K- cat ::Frt5) Δ endA ::Frt uidA (ΔMluI):: pir att HK::pJK1006Δ ( ori R6K- cat ::Frt5 trfA ::Frt) [ 58 ] E. coli BW25113 (pKD46) (F - (φ80dΔ( lacZ )M15 recA1 endA1 gyrA96 thi1 deoR ( lacZYA-argF )U169); λ bet- exo -gam; aph (Km r ) J. Beckwith, HMS S. ghanaensis ATCC14672 Wild-type moenomycin producer ATCC S. ghanaensis B38.3 Markerless deletion of moeH5 ; produces NoA [ 21 ] S. ghanaensis HIM5 Himar1 insertion into ssfg_01967 gene of B38.3 This work Bacillus cereus ATCC19637 Moenomycin-sensitive test culture ATCC pTES Am r ; phiC31-based Streptomyces integrative vector; expression of cloned gene from ermEp * [ 59 ] pKC1132 Am r ; conjugative suicide vector for gene disruption [ 14 ] pIJ6902 Am r Th r ; phiC31-based Streptomyces integrative vector; expression of cloned gene from tipAp [ 60 ] pHYG pLitmus-derived vector containing hygromycin resistance cassette hyg ; Hy r Ap r J. Salas, Universidad de Oviedo patt-shyg hygromycin cassette flanked by B-CC and P-GG sites for IMES recombination system [ 61 ] pGUS Promoter probe vector, contains promoterless gusA ; derived from pSET152 [ 20 ] pGUSHL4aadA pGUS derivative for translational fusions [ 20 ] padpAscript pGUS derivative, transcriptional fusion of adpAp (0.5 kb KpnI-XbaI fragment) to gusA , Am r Sp r [ 8 ] padpAtransl pGUSHL4aadA derivative, adpA-gusA translational fusion, Am r Sp r [ 8 ] padpAcontr pGUSHL4aadA, promoterless adpA gh -gusA fusion, Am r Sp r [ 8 ] pmiaB pKC1132 carrying 5 kb BamHI-HindIII fragment centred on |