pbluescript sk (Addgene inc)
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Addgene inc
pbluescript sk
Pbluescript Sk, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescript+sk/pBluescript+SK+%2B%2F-+mTRF1+(Plasmid+%2312303)/pm41290596-316-4-11
Average 93 stars, based on 39 article reviews
Pbluescript Sk, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescript+sk/pBluescript+SK+%2B%2F-+mTRF1+(Plasmid+%2312303)/pm41290596-316-4-11
Average 93 stars, based on 39 article reviews
pbluescript sk - by Bioz Stars,
2026-10
93/100 stars
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Plasmid Preparation:Article Title: The anti-leprosy drug clofazimine reduces polyQ toxicity through activation of PPARγ. Article Snippet: PINTOpolyQ94-GFP was cloned using pBlueScript SK-polyQ94 and pINTO-N-GFP using the KpnI and NotI sites. pcDNA3.1-EGFP-poly94 was cloned using pcDNA3.1mCherry (Addgene, #128744)33 and pINTO-polyQ94GFP with AflII and NotI sites. .. To clone the plasmid for SH-SY5Y cell infection, polyQ94-CFP was cloned into Article Title: Harmonizing TREC Thresholds in Newborn Screening for SCID: Insights From Russian Validation Cohort. Article Snippet: .. Human albumin, δREC–ψJα T cell receptor, and kappa- deleting joint of KREC were PCR amplified from DNA obtained from the peripheral blood of a healthy donor and separately cloned into the Article Title: The SMC Hinge is a Selective Gate for Obstacle Bypass Article Snippet: .. A phagemid based on Article Title: The SMC Hinge is a Selective Gate for Obstacle Bypass Article Snippet: .. A phagemid based on Article Title: The anti-leprosy drug clofazimine reduces polyQ toxicity through activation of PPARγ Article Snippet: PINTO-polyQ94-GFP was cloned using pBlueScript SK-polyQ94 and pINTO-N-GFP using the KpnI and NotI sites. pcDNA3.1-EGFP-poly94 was cloned using pcDNA3.1-mCherry (Addgene, #128744) and pINTO-polyQ94-GFP with AflII and NotI sites. .. To clone the plasmid for SH-SY5Y cell infection, polyQ94-CFP was cloned into Article Title: Exploring the importance of predicted camel NRAP exon 4 for environmental adaptation using a mouse model Article Snippet: .. Both the amplified ssODNs and the backbone vector, Article Title: Transgenic Lines of Human Induced Pluripotent Stem Cells ICGi022-A-6 and ICGi022-A-7 with Doxycycline-Inducible Variants of Programmable Nuclease AsCas12a Article Snippet: Genome editing in human pluripotent stem cells using programmable nucleases makes it possible to create models of hereditary pathologies using directed transgenesis, gene knockout, and replacement of individual nucleotides in DNA sequences.. Using CRISPR/SpCas9-mediated homologous recombination at the AAVS1 locus, clones of human induced pluripotent stem cells (iPSCs) ICGi022-A (Malakhova et al., 2020) were obtained, which carry transgenes of two variants of the nuclease AsCas12a (also known as AsCpf1), recognizing different PAM consensuses, and the reverse doxycycline transgene-dependent transactivator M2rtTA.. For each AsCas12a variant, the lines ICGi022-A-6 (AsCas12a, PAM 5'-TTTV-3') and ICGi022-A-7 (AsCas12a, PAM 5'-TYCV-3') were obtained. Article Title: The SMC Hinge is a Selective Gate for Obstacle Bypass. Article Snippet: .. A phagemid based on Infection:Article Title: The anti-leprosy drug clofazimine reduces polyQ toxicity through activation of PPARγ. Article Snippet: PINTOpolyQ94-GFP was cloned using pBlueScript SK-polyQ94 and pINTO-N-GFP using the KpnI and NotI sites. pcDNA3.1-EGFP-poly94 was cloned using pcDNA3.1mCherry (Addgene, #128744)33 and pINTO-polyQ94GFP with AflII and NotI sites. .. To clone the plasmid for SH-SY5Y cell infection, polyQ94-CFP was cloned into Article Title: The anti-leprosy drug clofazimine reduces polyQ toxicity through activation of PPARγ Article Snippet: PINTO-polyQ94-GFP was cloned using pBlueScript SK-polyQ94 and pINTO-N-GFP using the KpnI and NotI sites. pcDNA3.1-EGFP-poly94 was cloned using pcDNA3.1-mCherry (Addgene, #128744) and pINTO-polyQ94-GFP with AflII and NotI sites. .. To clone the plasmid for SH-SY5Y cell infection, polyQ94-CFP was cloned into Clone Assay:Article Title: The anti-leprosy drug clofazimine reduces polyQ toxicity through activation of PPARγ. Article Snippet: PINTOpolyQ94-GFP was cloned using pBlueScript SK-polyQ94 and pINTO-N-GFP using the KpnI and NotI sites. pcDNA3.1-EGFP-poly94 was cloned using pcDNA3.1mCherry (Addgene, #128744)33 and pINTO-polyQ94GFP with AflII and NotI sites. .. To clone the plasmid for SH-SY5Y cell infection, polyQ94-CFP was cloned into Article Title: Harmonizing TREC Thresholds in Newborn Screening for SCID: Insights From Russian Validation Cohort. Article Snippet: .. Human albumin, δREC–ψJα T cell receptor, and kappa- deleting joint of KREC were PCR amplified from DNA obtained from the peripheral blood of a healthy donor and separately cloned into the Article Title: The anti-leprosy drug clofazimine reduces polyQ toxicity through activation of PPARγ Article Snippet: PINTO-polyQ94-GFP was cloned using pBlueScript SK-polyQ94 and pINTO-N-GFP using the KpnI and NotI sites. pcDNA3.1-EGFP-poly94 was cloned using pcDNA3.1-mCherry (Addgene, #128744) and pINTO-polyQ94-GFP with AflII and NotI sites. .. To clone the plasmid for SH-SY5Y cell infection, polyQ94-CFP was cloned into Article Title: Transgenic Lines of Human Induced Pluripotent Stem Cells ICGi022-A-6 and ICGi022-A-7 with Doxycycline-Inducible Variants of Programmable Nuclease AsCas12a Article Snippet: Genome editing in human pluripotent stem cells using programmable nucleases makes it possible to create models of hereditary pathologies using directed transgenesis, gene knockout, and replacement of individual nucleotides in DNA sequences.. Using CRISPR/SpCas9-mediated homologous recombination at the AAVS1 locus, clones of human induced pluripotent stem cells (iPSCs) ICGi022-A (Malakhova et al., 2020) were obtained, which carry transgenes of two variants of the nuclease AsCas12a (also known as AsCpf1), recognizing different PAM consensuses, and the reverse doxycycline transgene-dependent transactivator M2rtTA.. For each AsCas12a variant, the lines ICGi022-A-6 (AsCas12a, PAM 5'-TTTV-3') and ICGi022-A-7 (AsCas12a, PAM 5'-TYCV-3') were obtained. Polymerase Chain Reaction:Article Title: Harmonizing TREC Thresholds in Newborn Screening for SCID: Insights From Russian Validation Cohort. Article Snippet: .. Human albumin, δREC–ψJα T cell receptor, and kappa- deleting joint of KREC were PCR amplified from DNA obtained from the peripheral blood of a healthy donor and separately cloned into the Amplification:Article Title: Harmonizing TREC Thresholds in Newborn Screening for SCID: Insights From Russian Validation Cohort. Article Snippet: .. Human albumin, δREC–ψJα T cell receptor, and kappa- deleting joint of KREC were PCR amplified from DNA obtained from the peripheral blood of a healthy donor and separately cloned into the Article Title: Exploring the importance of predicted camel NRAP exon 4 for environmental adaptation using a mouse model Article Snippet: .. Both the amplified ssODNs and the backbone vector, Transformation Assay:Article Title: The SMC Hinge is a Selective Gate for Obstacle Bypass Article Snippet: .. A phagemid based on Article Title: The SMC Hinge is a Selective Gate for Obstacle Bypass Article Snippet: .. A phagemid based on Article Title: The SMC Hinge is a Selective Gate for Obstacle Bypass. Article Snippet: .. A phagemid based on Construct:Article Title: Exploring the importance of predicted camel NRAP exon 4 for environmental adaptation using a mouse model Article Snippet: .. Both the amplified ssODNs and the backbone vector, |